• Communications of the Korean Mathematical Society
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• v.26 no.3
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• pp.385-403
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• 2011

Generalizations of the notion of fuzzy hyper K-subalgebras are considered. The concept of fuzzy hyper K-subalgebras of type (${\alpha},{\beta}$) where ${\alpha}$, ${\beta}$ ${\in}$ {${\in}$, q, ${\in}{\vee}q$, ${\in}{\wedge}q$} and ${\alpha}{\neq}{\in}{\wedge}q$. Relations between each types are investigated, and many related properties are discussed. In particular, the notion of (${\in}$, ${\in}{\vee}q$)-fuzzy hyper K-subalgebras is dealt with, and characterizations of (${\in}$, ${\in}{\vee}q$)-fuzzy hyper K-subalgebras are established. Conditions for an (${\in}$, ${\in}{\vee}q$)-fuzzy hyper K-subalgebra to be an (${\in}$, ${\in}$)-fuzzy hyper K-subalgebra are provided. An (${\in}$, ${\in}{\vee}q$)-fuzzy hyper K-subalgebra by using a collection of hyper K-subalgebras is established. Finally the implication-based fuzzy hyper K-subalgebras are discussed.

• IMMUNE NETWORK
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• v.10 no.6
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• pp.206-211
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• 2010

Background: Dendritic cell (DC)-based tumor vaccine is an attractive modality for the treatment of colon cancer because it has been recurred and produced few side effects in patients. Secretory glycoprotein 90K has been found at elevated level in various cancer tissues and sera. We investigated to establish a more effective DC vaccine for the treatment of colon cancer in which the levels of 90K are elevated. Methods: We obtained the concentrated 90K from 293T cells stably expressing 90K. DCs were cultured from peripheral blood monocytes, and a DC vaccine pulsed with tumor lysate was compared with a DC vaccine pulsed with 90K. We measured the functional activity for CTLs by using IFN-${\gamma}$-enzyme linked immunoabsorbent spot (ELISPOT) assay. Results: DCs pulsed with tumor lysate+90K exhibited the enhanced T cell stimulation, polarization of $\ddot{i}$ T cell toward Th1. The CTLs generated by DCs pulsed with 90K efficiently lysed HCT116 cells. The results indicate that 90K-speicifc-CTLs can recognize 90K proteins naturally presented by colon cancer cells. Conclusion: Our study suggests that 90K-specific CTLs generated by 90K-pulsed DCs could be useful effector cells for immunotherapy in colon cancer.

• Analytical Science and Technology
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• v.9 no.4
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• pp.411-415
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• 1996

The new potentially heptadentate $N_7$ ligand 1, 15-bis(2-pyridyl)-2, 5, 8, 11, 14-pentaazapentadecane(pytetren) has been synthesized and characterized by EA, IR, NMR, and mass spectrometry. Its proton association constants (log $K{_H}^n$) and stability constants(log $K_{ML}$) for $Co^{2+}$, $Ni^{2+}$, $Cu^{2+}$, and $Zn^{2+}$ ions were determined at 298.1K and ionic strength=0.100M($KNO_3$) by potentiometry: log $K{_H}^1=9.36$, log $K{_H}^2=9.12$, log $K{_H}^3=8.09$, log $K{_H}^4=6.62$, log $K{_H}^5=4.02$, log $K{_H}^6=2.54$: log $K_{ML}(CO^{2+})=22.67$, log $K_{ML}(Ni^{2+})=26.25$. log $K_{ML}(Cu^{2+})=28.46$, log $K_{ML}(Zn^{2+})=19.90$.

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2007.10a
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• pp.614-614
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• 2007

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2007.10a
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• pp.585-585
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• 2007

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2007.10a
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• pp.557-557
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• 2007

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2007.10a
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• pp.559-559
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• 2007

• Molecules and Cells
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• v.23 no.2
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• pp.239-245
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• 2007

DnaK is a major antigen in Streptococcus pneumoniae, and is induced by a minor shift in temperature (30 to $37^{\circ}C$) but not by ethanol shock. Although HrcA in the presence of $Ca^{{+}{+}}$ represses the expression of both groEL and hrcA, the control of transcription of the dnaK operon is not completely understood. In this study, the dnaK operon of S. pneumoniae (5' hrcA-grpE-dnaK-dnaJ) was cloned and analyzed. It contains large intergenic regions in grpE/dnaK and dnaK/dnaJ. Pulse labeling with [$^{35}S$]-methionine and immunoblot analyses revealed the presence of higher levels of DnaK than of HrcA even in the presence of $Ca^{{+}{+}}$ after heat shock suggesting that $Ca^{{+}{+}}$ differentially regulates the heat shock responses of hrcA and dnaK. By blocking de novo mRNA synthesis with rifampin it was shown that neither the hrcA nor the groEL transcripts were stabilized by heat shock even though dnaK transcripts were stabilized. We conclude that S. pneumoniae uses fine regulation of the transcription of the individual genes of the tetracistronic dnaK operon to cope with the various stresses experienced during infections.

• Journal of Microbiology and Biotechnology
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• v.23 no.2
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• pp.149-155
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• 2013

Heterologous expression of a putative $K^+/H^+$ antiporter of Streptomyces coelicolor A3(2) (designated as sha4) in E. coli and Streptomyces hygroscopicus JCM4427 showed enhanced tolerance to $K^+$ stress, acidic-pH shock, and/or geldanamycin production under $K^+$ stress. In a series of $K^+$ extrusion experiments with sha4-carrying E. coli deficient in the $K^+/H^+$ antiporter, a restoration of impaired $K^+$ extrusion activity was observed. Based on this, it was concluded that sha4 was a true $K^+/H^+$ antiporter. In different sets of experiments, the sha4-carrying E. coli showed significantly improved tolerances to $K^+$ stresses and acidic-pH shock, whereas sha4-carrying S. hygroscopicus showed an improvement in $K^+$ stress tolerance only. The sha4-carrying S. hygroscopicus showed much higher geldanamycin productivity than the control under $K^+$ stress condition. In another set of experiments with a production medium, the secretion of geldanamycin was also significantly enhanced by the expression of sha4.

• The Bulletin of The Korean Astronomical Society
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• v.34 no.1
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• pp.193-193
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• 2009