• Molecules and Cells
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• 제25권1호
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• pp.64-69
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• 2008

Although the arthritis symptoms observed in the K/BxN model have been shown to be dependent on the functions of T and B cells specific to the self antigen glucose-6-phosphate isomerase, less is known about the in vivo roles of $CD4^{+}CD25^{+}$ regulatory T($T_{reg}$) cells in the pathology of K/BxN mice. We determined the quantitative and functional characteristics of the $T_{reg}$ cells in K/BxN mice. These mice contained a higher percentage of $Foxp3^+\;T_{reg}$ cells among the $CD4^+$ T cells than their BxN littermates. These $T_{reg}$ cells were anergic and efficiently suppressed the proliferation of $na\ddot{i}ve$ $CD4^+$ T cells and cytokine production by effector $CD4^+$ T cells in vitro. Antibody-mediated depletion of $CD25^+$ cells caused K/BxN mice to develop multi-organ inflammation and autoantibody production, while the symptoms of arthritis were not affected. These results demonstrate that despite the inability of the $T_{reg}$ cells to suppress arthritis development, they play a critical role protecting the arthritic mice from systemic expansion of autoimmunity.

• IMMUNE NETWORK
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• 제11권5호
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• pp.299-306
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• 2011

Background: $CD4^+Fop3^+$ regulatory T cells (Tregs) are needed to maintain peripheral tolerance, but their role in the development of autoimmune arthritis is still debated. The present study was undertaken to investigate the mechanism by which Tregs influence autoimmune arthritis, using a mouse model entitled K/BxN. Methods: We generated Treg-deficient K/BxNsf mice by congenically crossing K/BxN mice with Foxp3 mutant scurfy mice. The arthritic symptoms of the mice were clinically and histopathologically examined. The proportions and activation of $CD4^+$ T cells and/or dendritic cells were assessed in the spleens, draining lymph nodes and synovial tissue of these mice. Results: K/BxNsf mice exhibited earlier onset and more aggressive progression of arthritis than their K/BxN littermates. In particular, bone destruction associated with the influx of numerous RANKL+ cells into synovia was very prominent. They also contained more memory phenotype $CD4^+$ T cells, more Th1 and Th2 cells, and fewer Th17 cells than their control counterparts. Plasmacytoid dendritic cells expressing high levels of CD86 and CD40 were elevated in the K/BxNsf synovia. Conclusion: We conclude that Tregs oppose the progression of arthritis by inhibiting the development of $RANKL^+$ cells, homeostatically proliferating $CD4^+$ T cells, Th1, Th2 and mature plasmacytoid dendritic cells, and by inhibiting their influx into joints.

• 한국식품저장유통학회지
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• 제13권6호
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• pp.669-674
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• 2006

청과물의 품질 영양표시 모델을 개발하기 위하여 경북의성군 옥자두를 대상으로 품질 및 영양적 성분을 분석하고 품질 영양표시 모델을 확립하였다. 일반성분은 수분 92%, 조단백 0.74%, 조지방 0.64%, 조섬유 1.65%, 조회분 0.32%, 무질소물 4.29%로 확인되었으며, 열량은 32kcal/100g 으로 계산되었다. 당도는 $10.33{\pm}0.85Brix^{\circ}$로, 산도는 $1.08{\pm}0.12%$로 나타났으며, 자두의 비타민 C 함량은 $7.037{\pm}0.317mg%$로 나타났다. 무기질은 황, 칼륨, 칼슘, 나트륨, 마그네슘 등의 순으로 함량이 높았고, 주요 유리당으로는 과당, 포도당, 자당로 나타났다. 유기산으로는 호박산, 사과산, 구연산이 확인되었다. 총 페놀화합물의 함량은 과육과 과피 간에 현저하게 차이를 보였으며 전과로는 $75.55{\pm}0.73mg%$의 함량을 보였다. 품질 표시에는 당과 산 함량, 영양 정보 표시에는 열량, 미네랄, 비타민 C, 총 페놀 성분이 표기된 자두의 품질 영양 표시 모델을 개발하여 제시하였다.