• 방송공학회논문지
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• 제26권3호
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• pp.269-282
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• 2021

증강현실과 가상현실 같은 3차원 콘텐츠 보급이 증가함에 따라 실시간 컴퓨터 애니메이션 기술의 중요성이 높아지고 있다. 하지만 컴퓨터 애니메이션 제작 과정은 대부분 수작업 혹은 마커를 부착하는 모션캡쳐 방식으로 이루어져 있다. 때문에 사실적인 영상을 얻기 위해서는 숙련된 전문가에게도 매우 오랜 시간이 필요하다. 이러한 문제점을 해결하기 위해 최근에는 딥러닝 모델과 센서를 기반으로 하는 애니메이션 제작 시스템과 알고리즘이 나오고 있다. 이에 본 논문에서는 딥러닝과 Kinect 카메라 기반 FBX 형식의 애니메이션 제작 시스템에서 자연스러운 인체 움직임을 구현하는 4가지 방법에 대해 연구했다. 각 방법은 환경적 특성과 정확도를 고려하여 선택된다. 첫 번째 방법은 Kinect 카메라를 사용한다. 두 번째 방법은 Kinect 카메라와 보정 알고리즘을 사용한다. 세 번째 방법은 딥러닝 모델을 사용한다. 네 번째 방법은 딥러닝 모델과 Kinect를 사용한다. 제안 방법을 오차와 처리 속도를 실험한 결과, 네 번째 딥러닝 모델과 Kinect를 동시에 사용하는 방법이 다른 방법에 비해 가장 좋은 결과를 보였다.

• Journal of Microbiology and Biotechnology
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• 제33권6호
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• pp.831-839
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• 2023

Tylosin is a potent veterinary macrolide antibiotic produced by the fermentation of Streptomyces fradiae; however, it is necessary to modify S. fradiae strains to improve tylosin production. In this study, we established a high-throughput, 24-well plate screening method for identifying S. fradiae strains that produce increased yields of tylosin. Additionally, we constructed mutant libraries of S. fradiae via ultraviolet (UV) irradiation and/or sodium nitrite mutagenesis. A primary screening of the libraries in 24-well plates and UV spectrophotometry identified S. fradiae mutants producing increased yields of tylosin. Mutants with tylosin yield 10% higher than the wild-type strain were inoculated into shake flasks, and the tylosin concentrations produced were determined by high-performance liquid chromatography (HPLC). Joint (UV irradiation and sodium nitrite) mutagenesis resulted in higher yields of mutants with enhanced tylosin production. Finally, 10 mutants showing higher tylosin yield were re-screened in shake flasks. The yield of tylosin A by strains UN-C183 (6767.64 ± 82.43 ㎍/ml) and UN-C137 (6889.72 ± 70.25 ㎍/ml) was significantly higher than that of the wild-type strain (6617.99 ± 22.67 ㎍/ml). These mutant strains will form the basis for further strain breeding in tylosin production.

• Journal of Acupuncture Research
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• 제21권4호
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• pp.177-196
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• 2004

Objective : Rheumatoid arthritis is an autoimmune disease that pathogenesis is not fully understood and one of the most intractable musculoskeletal diseases. The concern in the immunopathogenesis of rheumatoid arthritis has been increased since 1980's and many immunotherapeutic agents including disease-modifying antirheumatic drugs (DMARDs) were developed and became the mainstay of treatment of rheumatoid arthritis. However, the cure of the disease has hardly been achieved. In oriental medicine, rheumatoid arthritis is related to Bi-Zheng(痺證), that presents pain, swelling, andlor loss of joint function as major clinical manifestations, and also known to be deeply involved in suppression of immune function related to weakness of Jung-Ki(正氣). The herbal medicine, empirically used, could be a potential resource of development of new immunotherapeutic agents for rheumatoid arthritis. Methods : We developed a search strategy using terms to include "rheumatoid arthritis and herbal medicine" combined with "Chinese medicine" and/or "Oriental medicine". The search was focused on experimental studies of herbal medicine (January 1999 to May 2004), which is known to have effects on immune function of patients with rheumatoid arthritis. Computerized search used Internet databases including KISS and RISS4U (Korea), CNKI (China), MOMJ (Main Oriental Medicine Journal, Japan), and PubMed. The articles were selected from journals of universities or major research institutes. Results : The literature search for experimental studies on effects of herbal medicine on immunity of rheumatoid arthritis retrieved a total of 21 articles (Korea; 8, China ; 12, Japan ; 1). Of 21 articles, 10 were related to single-drug formula, 2 to drug interaction, and 9 to multi-drug formula. Single-drug formula was mainly used for aqua-acupuncture and researches on active components. Studies of drug interaction emphasized harmony of Ki-Hyul(氣血) and balance of Han-Yeul(寒熱). Multi-drug regimen was mainly found among formulas for Bo-Ki-Hyul(補氣血) and Bo-Sin(補腎). Conclusion : Studies on rheumatoid arthritis were performed both in vitro and in vivo in vitro study, LPS-stimulated splenocytes and synoviocytes were treated with herbal medicine, resulting in proliferation and activation of immune cells and suppression of cytokine activities in vivo study CIA animal model demonstrated that herbal medicine decreased antibody production and improved function of immune cells. In cellular and molecular study herbal medicine showed profound effects on the level of mRNA expression of certain cytokines related to immune function. This study revealed that herbal medicine has significant immune modulatory action and could be used for recovery of immune dysfunction of rheumatoid arthritis patients.

• Journal of Microbiology
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• 제44권5호
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• pp.548-555
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• 2006

FaeG is the key factor in the infection process of K88ad enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin. In an attempt to determine the possibility of expressing recombinant FaeG with immunogenicity for a new safe and high-production vaccine in E. coli, we constructed the recombinant strain, BL21 (DE3+K88), which harbors an expression vector with a DNA fragment of faeG, without a signal peptide. Results of 15% SDS-polyacrylamide slab gel analysis showed that FaeG can be stably over-expressed in BL21 (DE3+K88) as inclusion bodies without FaeE. Immunoglobulin G (IgG) and M (IgM) responses in pregnant pigs, with boost injections of the purified recombinant FaeG, were detected 4 weeks later in the sera and colostrum. An in vitro villius-adhesion assay verified that the elicited antibodies in the sera of vaccinated pigs were capable of preventing the adhesion of K88ad ETEC to porcine intestinal receptors. The protective effect on the mortality rates of suckling piglets born to vaccinated mothers was also observed one week after oral challenge with the virulent ETEC strain, $C_{83907}$ (K88ad, $CT^+,\;ST^+$). The results of this study proved that the adhesin of proteinaceous bacterial fimbriae or pili could be overexpressed in engineered E. coli strains, with protective immune responses to the pathogen.

• 대한한의학회지
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• 제34권3호
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• pp.69-85
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• 2013

Objectives: This study investigated the anti-osteoarthritic effects of Kyejiinsam-tang (hereinafter referred to KIT) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods: Anti-oxidative effects of KIT were measured by scavenging activities of DPPH, reactive oxygen species (ROS) and nitric oxide (NO). Scavenging activities of anti-oxidation in lipopolysaccharide (LPS)-treated RAW 264.7 cells were also measured for inhibitory effects against the production of inflammatory mediators (tumor necrosis factor-${\alpha}$, interleukin-$1{\beta}$, interleukin-6). Osteoarthritis was induced in rats by injecting MIA in the knee joint. Rats were divided into a total of 4 groups (n=6). The normal group were not treated at all without inducing osteoarthritis whereas the control group were induced for osteoarthritis by MIA and oral medicated physiological saline per day. The positive comparison group was injected with MIA and after 7 days, 2 mg/kg of Indomethacin. The experimental group was injected with MIA and after 7 days was medicated with 34 mg/kg of KIT. Indomethacin and KIT were orally-medicated for each substance a total of 4 weeks, once per day. Weight-bearing on hind legs was measured every week after MIA injection. At the end of the experiment (5 weeks after MIA injection), micro CT (computed tomography)-arthrography and histopathological examinations on the articular structures of knee joint were performed. The effect on inflammatory cytokines and immunological cells in synovial fluid was measured. Volume of cartilage was measured by micro CT-arthrography. Injury to synovial tissue was measured by H & E (hematoxylin and eosin), Safranin-O immunofluorescence. Results: 1. Cytotoxicity against hFCs was insignificant. 2. KIT showed the potent full term for DPPH. 1. NO was significantly reduced by KIT (at 100, $200{\mu}g/m{\ell}$) and ROS was also reduced, but not significantly, by KIT (at $200{\mu}g/m{\ell}$). 2. IL-6 and IL-$1{\beta}$ were significantly reduced by KIT (at 100, $200{\mu}g/m{\ell}$) and TNF-${\alpha}$ was also reduced, but not significantly, by KIT (at $200{\mu}g/m{\ell}$). 1. In hind legs weight-bearing measurement, level of weight increased. 2. Functions of liver and kidney were not affected. 3. IL-$1{\beta}$ was significantly reduced and TNF-${\alpha}$, IL-6 were also reduced but not significantly. 4. PGE2 (prostaglandin E2), LTB4 (leukotriene B4) were significantly reduced in the KIT group. 5. MMP-9 (matrix metalloproteinase-9), TIMP-1 (tissue inhibitor of metalloproteinases-1) and Osteocalcin were significantly reduced in the KIT group. 6. Destruction of cartilage on micro CT arthrography was reduced but had no significant differences. 7. Histopathologically, injury to synovial membrane of the KIT group was decreased and proteoglycan content of KIT group was increased. Conclusions: According to this study, Kyejiinsam-tang has inhibiting effect on the progression of arthritis in MIA-induced osteoarthritis rat. Kyejiinsam-tang has anti-oxidants and anti-inflammation effects, and is related to inhibiting the activity of inflammatory cytokine and injury of volume in cartilage.

• 한국도서관정보학회지
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• 제45권4호
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• pp.457-482
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• 2014

이 연구의 목적은 창간 40년을 맞은 "한국도서관 정보학회지"에 수록된 논문의 전수조사를 통해 주요 연구경향을 분석하는 것이다. 분석대상은 1974년에서 2013년까지 "한국도서관 정보학회지"에 수록된 1,365편의 논문이며, 분석기준은 연구주제, 공동연구 현황, 연구비 지원 유무, 연구자 소속지역 등이다. 분석결과는 다음과 같다. 첫째, 연구주제는 문헌정보학일반이 452편으로 가장 높았으며, 다음은 자료조직 213편, 정보서비스 188편, 정보학 155편, 독서지도 101편, 도서관경영 91편, 서지학 84편, 장서개발 58편, 기록관리학 20편으로 나타났다. 이를 통해 정보학은 증가추세인 반면 서지학은 감소추세며, 기록관리학은 생산량이 부진한 것으로 분석되었다. 둘째, 연구비 지원 여부는 428편이 연구비를 지원 받았으며, 이중 315편은 교내지원인 반면 나머지 108편은 정부지원으로 나타났다. 셋째, 공동연구의 경향은 점점 높아지는 추세에 있으며, 전무했던 초창기와 달리 최근에는 303편으로 증가하였다. 넷째, 연구자 소속지역은 대구, 경북에 집중되었던 초창기 현상과 달리 최근 들어서는 전국적으로 고른 분포를 나타냈다. 이를 통해 지역기반학회지에서 전국규모 학회지로 성장하였음을 알 수 있다.

• 기술혁신학회지
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• 제18권1호
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• pp.28-48
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• 2015

공공 R&D 연구기관은 기업에게 좋은 기술공급처다. 그러나 낮은 사업화 성공률은 기업에게 고민꺼리다. 어떤 기술을 이전 받을 때 사업화 성공률을 높일 수 있을까? 본 연구에서는 대표적인 한국의 IT기술분야 공공R&D 기관을 대상으로 이전기술의 사업화 성공요인을 로지스틱으로 회귀분석했다. 분석 결과, 이전기술이 기업의 기존보유 제품과 연계성이 높고, 기술적 호환성이 높을수록 사업화 성공률이 높았다. 반면, 예상과는 달리 이전받을 기술의 개발과정에 기업이 공동연구로 참여한 경우보다는 독립적으로 기술이전 받을 때 오히려 상용화 성공률이 높게 나타났으며, 이전기술의 완성도는 유의한 영향을 미치지 않았다.

• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2010년도 춘계학술발표대회 초록집
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• pp.51-51
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• 2010

Recently just as in the automobile industry, shipbuilders also try to reduce material consumption and weight in order to keep operating costs as low as possible and improve the speed of production. Naturally industry is ever searching for welding techniques offering higher power, higher productivity and a better quality. Therefore it is important to have a details research based on the various welding process applied to steel and other materials, and to have the ability both to counsel interested companies and to evaluate the feasibility of implementation of this process. Submerged-arc welding (SAW) process is usually used about 20% of shipbuilding. Similar to gas metal arc welding(GMAW), SAW involves formation of an arc between a continuously-fed bare wire electrode and the work-piece. The process uses a flux to generate protective gases and slag, and to add alloying elements to the weld pool and a shielding gas is not required. Prior to welding, a thin layer of flux powder is placed on the work-piece surface. The arc moves along the joint line and as it does so, excess flux is recycled via a hopper. Remaining fused slag layers can be easily removed after welding. As the arc is completely covered by the flux layer, heat loss is extremely low. This produces a thermal efficiency as high as 60% (compared with 25% for manual metal arc). SAW process offers many advantages compared to conventional CO2 welding process. The main advantages of SAW are higher welding speed, facility of workers, less deformation and better than bead shape & strength of welded joint because there is no visible arc light, welding is spatter-free, fully-mechanized or automatic process, high travel speed, and depth of penetration and chemical composition of the deposited weld metal. However it is difficult to application of thin plate according to high heat input. So this paper has been focused on application of the field according to SAW process for thin plate in ship-structures. For this purpose, It has been decided to optimized welding condition by experiments, relationship between welding parameters and bead shapes, mechanical test such as tensile and bending. Also finite element(FE) based numerical comparison of thermal history and welding residual stress in A-grade 3.2 thickness steel of SAW been made in this study. From the result of this study, It makes substantial saving of time and manufacturing cost and raises the quality of product.

• 한국전산구조공학회논문집
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• 제30권6호
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• pp.531-539
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• 2017

전통적인 FRP 선체 제작에는 mould가 요구되며, mould의 높은 제작비용은 FRP 선박 제작자에게 비용부담을 주고 있다. 이를 극복하기 위한 방안으로 강선 건조에서 사용되는 블록 혹은 모듈 방식의 제작기법에 착안한 modular construction 방안이 제시되었다. 이 제작방안을 FRP 선체에 효율적으로 적용하기 위해서는 설계 및 제작 측면에서 간략하면서도 구조적 안정성을 갖는 접합 이음부 개발이 이루어져야 한다. 따라서, 본 논문에서는 모듈방식의 FRP 선체 제작을 위해 moulding-in 개념을 기반으로 한 경제적인 접합 이음부 개발에 관한 연구를 수행하였다. FRP 샌드위치 판을 대상으로 step, scarf-step 그리고 scarf 이음부 형상을 갖는 moulded-in을 수지주입식진공성형 공정에 도입하여 성공적으로 접합된 FRP 샌드위치 판을 제작하였다. 이에 대해 구조적 안정성 평가를 목적으로 인장 및 4점 굽힘 시험 그리고 유한요소해석을 수행하였다. 시험과 해석결과에 대한 비교 연구를 통해 본 연구에서 개발된 접합 이음 내용이 모듈방식의 FRP 선체 제작에 적용 가능함을 확인할 수 있었다.

• 한국시뮬레이션학회논문지
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• 제26권3호
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• pp.115-123
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• 2017

우리 군이 그동안 전차, 자주포, 미사일 등 주요 무기체계를 개발하여 전력화 하면서 외형적으로 괄목할만한 성과를 이루어 왔음에도 불구하고, 개발된 무기가 실제 전장에서 어떤 효과를 발휘할 것인가에 대한 척도를 제시해 주는 무기효과자료를 산출하는 것에 대해서는 관심이 부족하였다. 이러한 이유로 아직까지 무기효과자료 산출을 위한 기반이 미흡하여, 무기효과자료는 대부분 미국으로부터 도입된 JMEM 자료를 활용하고 있고 미 확보된 자료에 대해서는 사용부서에서 각자 연구하여 데이터를 생산, 활용하고 있다. 따라서 본 연구에서는 기존에 수행되어 온 무기효과 산출방법론을 연구하여 간편하면서도 적은 예산으로 신뢰성있는 무기효과자료를 산출할 수 있는 방법을 도출하는 데 중점을 두었다. 미국의 JMEM 방법론으로부터 식별한 절차 및 알고리즘을 활용하고, 현재 한국군이 보유하고 있는 유사 무기체계의 무기효과자료를 기초로 통계 및 시뮬레이션기법을 복합적으로 적용하여 직사화기 중 대표화기인 전차를 중심으로 살상확률(Pk)을 계산하는 방법론을 제시하였다.