• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.41-41
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• 2015

BSA-seq technologies, combined Bulked Segregant Analysis (BSA) and Next-Generation Sequencing (NGS), are making it faster and more efficient to establish the association of agronomic traits with molecular markers or candidate genes, which is the requirement for marker-assisted selection in molecular breeding. Clubroot disease, caused by Plasmodiophora brassicae, is a serious threat to Brassica crops. Even we have breed new clubroot resistant varieties of Chinese cabbage (B. rapa ssp. pekinesis), the underlying genetic mechanism is unclear. In this study, an $F_2$ population of 340 plants were inoculated with P. brassicae from Xinye (Pathotype 2 on the differentials of Williams). Resistance phenotype segregation ratio for the populations fit a 3:1 (R:S) segregation model, consistent with a single dominant gene model. Super-BSA, using re-sequencing the parents, extremely R and S DNA pools with each 50 plants, revealed 3 potential candidate regions on the chromosome A03, with the most significant region falling between 24.30 Mb and 24.75 Mb. A linkage map with 31 markers in this region was constructed with several closely linked markers identified. A Major QTL for clubroot resistance, CRq, which was identified with the peak LOD score at 169.3, explaining 89.9% of the phenotypic variation. And we developed a new co-segregated InDel marker BrQ-2. Joint BSA-seq and traditional QTL analysis delimited CRq to an 250 kb genomic region, where four TIR-NBS-LRR genes (Bra019409, Bra019410, Bra019412 and Bra019413) clustered. The CR gene CRq and closely linked markers will be highly useful for breeding new resistant Chinese cabbage cultivars.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.16
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• pp.7205-7210
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• 2015

Background: In this study, influence caused by expression plasmids of connective tissue growth factor (CTGF) and tissue inhibitor of metalloproteinase-1 (TIMP-1) short hairpin RNA (shRNA) on mRNA expression of CTGF,TIMP-1,procol-${\alpha}1$ and PCIII in hepatic tissue with hepatic fibrosis, a precancerous condition, in rats is analyzed. Materials and Methods: To screen and construct shRNA expression plasimid which effectively interferes RNA targets of CTGF and TIMP-1 in rats. 50 cleaning Wistar male rats are allocated randomly at 5 different groups after precancerous fibrosis models and then injection of shRNA expression plasimids. Plasmid psiRNA-GFP-Com (CTGF and TIMP-1 included), psiRNA-GFP-CTGF, psiRNA-GFP-TIMP-1 and psiRNA-DUO-GFPzeo of blank plasmid are injected at group A, B, C and D, respectively, and as model control group that none plasimid is injected at group E. In 2 weeks after last injection, to hepatic tissue at different groups, protein expression of CTGF, TIMP-1, procol-${\alpha}1$ and PC III is tested by immunohistochemical method and,mRNA expression of CTGF,TIMP-1,procol-${\alpha}1$ and PCIII is measured by real-time PCR. One-way ANOVA is used to comparison between-groups. Results: Compared with model group, there is no obvious difference of mRNA expression among CTGF,TIMP-1,procol-${\alpha}1$, PC III and of protein expression among CTGF, TIMP-1, procol-${\alpha}1$, PC III in hepatic tissue at group injected with blank plasmid. Expression quantity of mRNA of CTGF, TIMP-1, procol-${\alpha}1$ and PCIII at group A, B and C decreases, protein expression of CTGF, TIMP-1, procol-${\alpha}1$, PC III in hepatic tissue is lower, where the inhibition of combination RNA interference group (group A) on procol-${\alpha}1$ mRNA transcription and procol-${\alpha}1$ protein expression is superior to that of single interference group (group B and C) (P<0.01 or P<0.05). Conclusions: RNA interference on CTGF and/or TIMP-1 is obviously a inhibiting factor for mRNA and protein expression of CTGF, TIMP-1, procol-${\alpha}1$ and PCIII. Combination RNA interference on genes of CTGF and TIMP-1 is superior to that of single RNA interference, and this could be a contribution for prevention of precancerous condition.

• International Commerce and Information Review
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• v.13 no.3
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• pp.31-53
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• 2011

The purpose of this study is to find out the factors affecting the usage and performance of e-Trade in Chinese Small and Medium-sized Enterprises(SMEs). To achieve the purpose of the study, the research model and hypotheses, depicting the relationship among external factors, internal factors, relationship characteristics, e-Trade usage level, and performance were developed based on the existing literature on e-Trade. To test the significance of the research model and hypotheses, an empirical survey were conducted to the Chinese SMEs in Yangtze River Delta area, which are actively using the e-Trade in their trade processes. According to the result of the statistical analyses on the 85 effective questionnaires from the sample firms, support of the government related institutions(one of the external factors) and CEO's support(one of the internal factors) had significant relationship with e-Trade usage level, but no significant relationship was found between the relationship characteristics and e-Trade usage level. Like the results of the most of the existing literature, e-Trade usage level were formed to significantly affected the e-Trade performance. Finally, based on results of the empirical survey, several implications on the Chinese SME's e-Trade strategies and policy alternatives were proposed as a concluding remark.

• JSTS:Journal of Semiconductor Technology and Science
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• v.16 no.1
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• pp.91-105
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• 2016

Carbon Nanotube Field-Effect Transistors (CNTFETs) have been studied as candidates for post Si CMOS owing to the better electrostatic control and high mobility. To enhance the immunity against short - channel effects (SCEs), the novel channel and gate engineered architectures have been proposed to improve CNTFETs performance. This work presents a comprehensive study of the influence of channel and gate engineering on the CNTFET switching, high frequency and circuit level performance of carbon nanotube field-effect transistors (CNTFETs). At device level, the effects of channel and gate engineering on the switching and high frequency characteristics for CNTFET have been theoretically investigated by using a quantum kinetic model. This model is based on two-dimensional non-equilibrium Green's functions (NEGF) solved self - consistently with Poisson's equations. It is revealed that hetero - material - gate and lightly doped drain and source CNTFET (HMG - LDDS - CNTFET) structure can significantly reduce leakage current, enhance control ability of the gate on channel, improve the switching speed, and is more suitable for use in low power, high frequency circuits. At circuit level, using the HSPICE with look - up table(LUT) based Verilog - A models, the impact of the channel and gate engineering on basic digital circuits (inverter, static random access memory cell) have been investigated systematically. The performance parameters of circuits have been calculated and the optimum metal gate workfunction combinations of ${\Phi}_{M1}/{\Phi}_{M2}$ have been concluded in terms of power consumption, average delay, stability, energy consumption and power - delay product (PDP). In addition, we discuss and compare the CNTFET-based circuit designs of various logic gates, including ternary and binary logic. Simulation results indicate that LDDS - HMG - CNTFET circuits with ternary logic gate design have significantly better performance in comparison with other structures.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.13
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• pp.5249-5252
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• 2014

Nogo protein, encoded by gene reticulon-4 (RTN4), includes three major isoforms by different splicing, named Nogo-A Nogo-B and Nogo-C. Nogo proteins play an important role in the apoptosis of cells, especially in tumor cells. RTN4 single nucleotide polymorphisms (SNPs) can influence the efficiency of transcription and translation thus being related with an individual's predisposition to cancer. The CAA insertion/deletion polymorphism (rs34917480) within RTN4 3'-UTR has been reported to be associated with many cancer types. In order to investigate the relationship between this polymorphism and susceptibility to non-small cell lung cancer (NSCLC) in the Chinese population, we conducted the present case-control study including 411 NSCLC patients and 471 unrelated healthy controls. The genotype distributions were significantly different between cases and controls (p=0.014). We found that the del allele could significantly increase NSCLC risk (ins/ins vs ins/del: p=0.007, OR 1.46, 95%CI=1.11-1.93; dominant model: p=0.004, OR 1.47, 95%CI=1.13-1.92 and allele model: p=0.008, OR 1.35, 95%CI=1.08-1.67). This association was stronger in participants over 60 years old, males and smokers. We therefore conclude that the CAA insertion/deletion polymorphism (rs34917480) contributes to non-small cell lung cancer risk in Chinese population. Age, sex and environmental exposure are also related to carcinogenic effects of rs34917480.

• Steel and Composite Structures
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• v.49 no.1
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• pp.81-89
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• 2023

A simplified calculation method of natural vibration characteristics of high-speed railway multi-span bridge-longitudinal ballastless track system is proposed. The rail, track slab, base slab, main beam, bearing, pier, cap and pile foundation are taken into account, and the multi-span longitudinal ballastless track-beam-bearing-pier-cap-pile foundation integrated model (MBTIM) is established. The energy equation of each component of the MBTIM based on Timoshenko beam theory is constructed. Using the improved Fourier series, and the Rayleigh-Ritz method and Hamilton principle are combined to obtain the extremum of the total energy function. The simplified calculation formula of the natural vibration frequency of the MBTIM under the influence of vertical and longitudinal vibration is derived and verified by numerical methods. The influence law of the natural vibration frequency of the MBTIM is analyzed considering and not considering the participation of each component of the MBTIM, the damage of the track interlayer component and the stiffness change of each layer component. The results show that the error between the calculation results of the formula and the numerical method in this paper is less than 3%, which verifies the correctness of the method in this paper. The high-order frequency of the MBTIM is significantly affected considering the track, bridge pier, pile soil and pile cap, while considering the influence of pile cap on the low-order and high-order frequency of the MBTIM is large. The influence of component damage such as void beneath slab, mortar debonding and fastener failure on each order frequency of the MBTIM is basically the same, and the influence of component damage less than 10m on the first fourteen order frequency of the MBTIM is small. The bending stiffness of track slab and rail has no obvious influence on the natural frequency of the MBTIM, and the bending stiffness of main beam has influence on the natural frequency of the MBTIM. The bending stiffness of pier and base slab only has obvious influence on the high-order frequency of the MBTIM. The natural vibration characteristics of the MBTIM play an important guiding role in the safety analysis of high-speed train running, the damage detection of track-bridge structure and the seismic design of railway bridge.

• Korean Journal of Radiology
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• v.20 no.5
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• pp.830-843
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• 2019

Objective: To examine the potential of intravoxel incoherent motion (IVIM) and blood oxygen level-dependent (BOLD) magnetic resonance imaging for detecting renal changes after iodinated contrast-induced acute kidney injury (CI-AKI) development in a diabetic rabbit model. Materials and Methods: Sixty-two rabbits were randomized into 2 groups: diabetic rabbits with the contrast agent (DCA) and healthy rabbits with the contrast agent (NCA). In each group, 6 rabbits underwent IVIM and BOLD imaging at 1 hour, 1 day, 2 days, 3 days, and 4 days after an iohexol injection while 5 rabbits were selected to undergo blood and histological examinations at these specific time points. Iohexol was administrated at a dose of 2.5 g I/kg of body weight. Further, the apparent transverse relaxation rate (R2^*), average pure molecular diffusion coefficient (D), pseudo-diffusion coefficient (D^*), perfusion fraction (f) were calculated. Results: The D and f values of the renal cortex (CO) and outer medulla (OM) were significantly decreased compared to baseline values in the 2 groups 1 day after the iohexol injection (p < 0.05). A marked reduction in the D^* values for both the CO and OM was also observed after 1 hour in each group (p < 0.05). In the OM, a persistent elevation of the R2^* was detected for 4 days in the DCA group (p < 0.05). Histopathological changes were prominent, and the pathological features of CI-AKI aggravated in the DCA group until day 4. The D, f, and R2^* values significantly correlated with the histological damage scores, hypoxia-inducible transcription factor-1α expression scores, and serum creatinine levels. Conclusion: A combination of IVIM and BOLD imaging may serve as a noninvasive method for detecting and monitoring CI-AKI in the early stages in the diabetic kidney.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.11
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• pp.1405-1411
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• 2010

Calpastatin (CAST), an endogenous inhibitor of the calpains, plays an important role in post-mortem tenderization of meat. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in the bovine CAST gene and association with carcass and meat quality traits. A total of 212 cattle from commercial herds were tested in this study including 2 pure introduced breeds, 4 cross populations, and 3 pure Chinese native breeds. Five SNPs were identified at position 2959 (A/G), 2870 (G/A), 3088 (C/T), 3029 (G/A) and 2857 (C/T) in the CAST gene (GenBank Accession No. AF159246). Allele frequencies of SNP2959 and SNP2870 were 0.701 (A) and 0.462 (A), respectively. A general linear model was used to evaluate the associations between the two markers and 7 traits. The results showed that both SNP2959 and SNP2870 were significantly (p<0.01) associated with the Warner-Bratzler shear force (WBSF), while they had no significant association with the other 6 traits in the whole population. However, in Chinese native pure breeds, only SNP2870 had significant association with WBSF (p<0.05). The simultaneous analysis of two-marker genotype effects indicated animals containing the A/G haplotype (A for SNP2959 and G for SNP2870) tended to have lower shear force than those containing the G/A haplotype, and, especially, animals homozygous for the A/G haplotype had approximately 2 kg lower shear force than those homozygous for the G/A haplotype (p<0.01). These results suggested that both markers may be effective for the marker-assisted selection of meat quality traits in Chinese commercial herds, especially SNP2870 which can be used for Chinese native cattle.

• Journal of the Korean earth science society
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• v.34 no.6
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• pp.524-535
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• 2013

Catastrophe risk models require the damage functions of each vulnerable item in inventory to estimate volcanic ash losses. The damage functions are used to represent the relation between damage factors and damage and also widely used in engineering and natural hazard studies to calculate the vulnerability. In most cases, damage functions are constructed as fragility or vulnerability curves, and researchers are confused by the similarities between them particularly when they perform interdisciplinary research. Thus, we aim to explain the similarities and differences between fragility and vulnerability curves and their relationship by providing case studies to construct them. In addition, we suggest a simple method to construct the damage functions between damage ratio and volcanic ash thickness using limited damage data. This study comes from the fact that damage functions are generally constructed using damage data. However, there is no available volcanic ash damage data in Korea, and not even enough volcanic disaster data to construct damage functions in the world, compared to other hazards. Using the method suggested in the study and the limited damage data from Japan and New Zealand, we construct Weibull-type functions or linear functions dependent of available data to calculate volcanic ash loss estimation, which we think need to be corrected to make it more suitable for inventory characteristics and environmental conditions in Korea.

• Journal of Electrical Engineering and Technology
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• v.13 no.6
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• pp.2402-2411
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• 2018

Fluoronitriles-$CO_2$ gas mixtures are promising alternatives to $SF_6$ in environmentally-friendly gas-insulated transmission lines (GILs). Insulating gas heat transfer characteristics are of major significance for the current-carrying capacity design and operational state monitoring of GILs. In this paper, a three-dimensional calculation model was established for a GIL using the thermal-fluid coupled finite element method. The calculated results showed close agreement with experimentally measured data. The temperature distribution of a GIL filled with the Fluoronitriles-$CO_2$ mixture was obtained and compared with those of GILs filled with $CO_2$ and $SF_6$. Furthermore, the effects of the mixture ratio of the component gases and the gas pressure on the temperature rise and current-carrying capacity of the GIL were analyzed. Results indicated that the heat transfer performance of the Fluoronitriles-$CO_2$ gas mixture was better than that of $CO_2$ but worse than that of $SF_6$. When compared with $SF_6$, use of the Fluoronitriles-$CO_2$ gas mixture caused a reduction in the GIL's current-carrying capacity. In addition, increasing the Fluoronitriles gas component ratio or increasing the pressure of the insulating gas mixture could improve the heat dissipation and current-carrying capacity of the GIL. These research results can be used to design environmentally-friendly GILs containing Fluoronitriles-$CO_2$ gas mixtures.