• 대한토목학회논문집
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• 제4권4호
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• pp.79-93
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• 1984

수중조류(水中藻類) 제거(除去)하기 위한 공법(工法)의 하나로 가압부상법(加壓浮上法)은 아주 효과적(効果的)이다. 이러한 가압부상법(加壓浮上法)의 효율(効率)에 영향을 미치는 요소(要素)로는 시료수(試料水)에 대(對)한 가압수(加壓水)의 체적비율(體積比率), 가압수(加壓水)의 압력(壓力), 접촉시간(接觸時間), 응집제(凝集劑)의 종류(種類) 및 투여량, 수온(水溫), 반응조내(反應槽內)의 물의 흐름상태, 기포(氣泡)의 크기 및 상승속도(上昇速度), 그리고 기포(氣泡)와 입자(粒子)간의 접착(接着) 등이다. 본(本) 연구(硏究)에 있어서는 모형조내(模型槽內)에서 실제의 기포(氣泡) 상승속도(上昇速度)와 이론적(理論的)인 상승속도(上昇速度)와의 비교, 기포(氣泡)와 입자(粒子)간의 접착현상(接着現象) 규명 기포(氣泡)의 크기 및 상승속도(上昇速度)가 수중조류(水中藻類) 제거공정(除去工程)에 미치는 영향 등을 규명함으로써 가압부상법(加壓浮上法)의 합리적(合理的)인 적용방법(適用方法)을 검토(檢討)하였다. 수중조류(水中藻類) 제거(除去)를 위하여 기포(氣泡)의 발생(發生)과 기포(氣泡)의 크기 변화(變化)과정 및 기포(氣泡)의 부상속도(浮上速度), 기포(氣泡)와 입자(粒子)간의 접착현상(接着現象), 연속식(連續式) 가압부상(加壓浮上) 실험(實驗)의 이론적(理論的) 고찰과 실험적(實驗的) 증명에 의(依)하여 얻은 결과(結果)는 다음과 같다. 기포(氣泡) 상승속도식(上昇速度式)은 스톡스 방정식(方程式)보다 아이브스 식(式)이 더 적합(適合)하다. 기포(氣泡)와 조류(藻類)와의 결합(結合)은 컨벡티브 타입이었으며 부착현상(附着現象)과 충돌현상(衝突現象)보다 흡수현상(吸收現象)에 의(依)한 접착(接着)이 많았다. 기포(氣泡)와 크기는 $100{\mu}m$ 보다 작으며 반응조내(反應槽內)의 유동(流動)이 적을 때가 처리효율(處理効率)이 좋았다. 또한, 본(本) 실험(實驗)에 사용(使用)된 연속식(連續式) 가압부상(加壓浮上) 장치의 최적조건(最適條件)으로는 시료수(試料水)에 대(對)한 가압수(加壓水)의 체적비(體積比)가 15%, 반응조내(反應槽內) 체류시간은 15분(分), 가압수(加壓水) 압력(壓力) $4kg/cm^2$, 가압수(加壓水) 분사기(噴射機)의 시료수(試料水) 유입구(流入口)와의 거리는 30cm 이었으며 온수(水溫)의 변화(變化)에 따른 처리효율(處理効率)의 변동은 거의 없었으며 처리효율(處理効率)은 85~91% 였다.

• International Journal of Ocean System Engineering
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• 제2권3호
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• pp.185-194
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• 2012

A Macroscopic combination of two or more distinct materials is commonly referred to as a "Composite Material", having been designed mechanically and chemically superior in function and characteristic than its individual constituent materials. Composite materials are used not only for aerospace and military, but also heavily used in boat/ship building and general composite industries which we are seeing increasingly more. Regardless of the various applications for composite materials, the industry is still limited and requires better fabrication technology and methodology in order to expand and grow. An example of this is that the majority of fabrication facilities nearby still use an antiquated wet lay-up process where fabrication still requires manual hand labor in a 3D environment impeding productivity of composite product design advancement. As an expert in the advanced composites field, I have developed fabrication skills with the use of machinery based on my past composite experience. In autumn 2011, the Korea government confirmed to fund my project. It is the development of a composite sanding machine. I began development of this semi-robotic prototype beginning in 2009. It has possibilities of replacing or augmenting the exhaustive and difficult jobs performed by human hands, such as sanding, grinding, blasting, and polishing in most often, very awkward conditions, and is also will boost productivity, improve surface quality, cut abrasive costs, eliminate vibration injuries, and protect workers from exposure to dust and airborne contamination. Ease of control and operation of the equipment in or outside of the sanding room is a key benefit to end-users. It will prove to be much more economical than normal robotics and minimize errors that commonly occur in factories. The key components and their technologies are a 360 degree rotational shoulder and a wrist that is controlled under PLC controller and joystick manual mode. Development on both of the key modules is complete and are now operational. The Korean government fund boosted my development and I expect to complete full scale development no later than 3rd quarter 2012. Even with the advantages of composite materials, there is still the need to repair or to maintain composite products with a higher level of technology. I have learned many composite repair skills on composite airframe since many composite fabrication skills including repair, requires training for non aerospace applications. The wind energy market is now requiring much larger blades in order to generate more electrical energy for wind farms. One single blade is commonly 50 meters or longer now. When a wind blade becomes damaged from external forces, on-site repair is required on the columns even under strong wind and freezing temperature conditions. In order to correctly obtain polymerization, the repair must be performed on the damaged area within a very limited time. The use of pre-impregnated glass fabric and heating silicone pad and a hot bonder acting precise heating control are surely required.

• Applied Microscopy
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• 제24권2호
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• pp.78-92
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• 1994

Lysozyme has been reported to be present in the secretory granules of the Paneth cell, and lysozyme immunoreactivity has been detected by immunogold method in Paneth cells of the intestine of human, mouse and rat. The present study was aimed at clarifying the intracellular distribution and changes of the lysozyme immunoreactivity in rabbit Paneth cell after common bile duct ligation of rabbit, using the electron microscope immunogold technique. Healthy adult rabbits weighing about 2kg body weight were divided into normal and bile duct ligated groups. Common bile duct ligation was performed aseptically under ether anesthesia. Experimental animals were sacrificed on the 1st, the 3rd, the 5th, the 7th and the 14th day after the operation. Mucosal specimens from the intestinal gland of ileum were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde, followed by 1% osmium tetroxide, embedded in araldite mixture, cut with LKB-V ultratome. Ultrathin sections were placed on parlodion coated nickel grids (200mesh). The section-bearing grids were floated upside down on the added substance in a moist chamber at room temperature except for the primary antibody step, which was at $4^{\circ}C$. Sections were etched with a saturated solution of sodium m-periodate for 60min. After etching, sections were pretreated with 0.02M tris buffered saline (TBS), pH 8.4, with 1% bovine serum albumin (BSA, Sigma) for 60min, then treated polyclonal rabbit anti-human lysozyme (Dakipatts) diluted 1 : 50 in TBS with 0.1% BSA for 20hr. Subsequently, grids were incubated 60min in biotinylated goat anti rabbit IgG (Amersham) diluted 1 : 100 in TBS with 0.1% BSA. After this, sections were incubated 60min on streptavidin gold G10 (Amersham) diluted 1 : 50 in TBS with 0.1% BSA. After each step, the grids were briefly rinsed with TBS with 0.1% BSA. After the strepavidin gold step, the sections were jet washed with distilled water. Counterstain of the sections performed by uranyl acetate and lead citrate, and observed with JEM 100 CX II electron microscope. Observed results were as follow; 1. Secretory granules of mouse Paneth cells have a lysozyme immunoreactivity and also eosinophil leucocyte of rabbit applied for the positive-control stain, are well labeld with gold particles. 2. Normal rabbit Paneth cells have a lysozyme immunoreactivity restricted on the secretory granules. 3. Amount lysosomes containing myelin figures in the Paneth cells were significantly increased from 5th day after the common bile duct ligation. 4. Immunoreactivity of Paneth cell secretory granules were more activated on the 3rd day after the common bile duct ligation as compared with those of the normal animal. But the lysozyme immunoreactivity were decreased from the 5th day after the common bile duct ligation. 5. Considering the above finding, lysozyme contained Paneth cell are affected following of common bile duct ligation, whereas lysosomes containing myelin-figure do not exhibit any immunoreactive relationship with those of secretory granules.

• 한국수정란이식학회지
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• 제30권3호
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• pp.195-200
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• 2015

본 연구에서 배아의 생식세포 동결에 가장 흔히 쓰이고 있는 침투성 동결보호제(DMSO, EG, Glycerol, 1,2-PROH)의 독성을 비교하고자 생쥐 수정란 모델을 이용한 실험을 하였다. 생후 6주령의 암컷 생쥐 F1 hybrid mice에 10 IU의 PMSG를 복강 주사하여 과배란을 유도하고, 2-세포기 배아를 획득하고 침투성 동결보호제(DMSO, EG, Glycerol와 1,2-PROH) 각각 실온에서 60분간 노출시킨 후, 배양을 하였다. 배반포의 전체 세포수는 2-세포기 단계에서 DMSO($68.1{\pm}24.1$), EG($68.1{\pm}24.1$), Glycerol($81.2{\pm}27.0$), 1,2-PROH($68.1{\pm}24.1$) 침투성 동결보호제 처리군은 대조군에 비해 유의적으로($99.0{\pm}18.3$)(p<0.001) 낮았다. DMSO와 Glycerol 처리구가 EG와 1,2-PROH 처리구에 비해 세포수가 적었다. DMSO($15.4{\pm}1.5$), EG($10.2{\pm}1.4$), Glycerol ($10.2{\pm}1.4$), 1,2-PROH($10.2{\pm}1.4$) 네 처리구는 대조구($6.1{\pm}0.9$, p<0.0001)와 비교해서 배반포에서 세포사 비율이 더 높음을 확인했다. 또한, DMSO와 Glycerol 처리구는 EG와 1,2-PROH 처리구(p<0.001)보다 더 많은 세포사멸된 세포가 각각 확인되었다. DMSO, EG, Glycerol과 1,2-PROH 처리군과 대조군 사이에는 배아 부화율에 있어서 차이가 있었으며 이는 배아에 대한 동결보호제의 잠재적인 독성을 확인한 결과였다. 이번 연구에서 장기간 처리했을 때 EG와 1,2-PROH 처리군보다 DMSO와 Glycerol 처리군에서 배아발달과 세포수가 저하된 것은 DMSO와 Glycerol의 독성이 더 높을 것으로 사료된다.