• Journal of Mushroom
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• v.22 no.1
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• pp.27-30
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• 2024

As a member of ectomycorrhizal fungi, Tricholoma matsutake has a symbiotic relationship with its host, Pinus densiflora. To cultivate T. matsutake artificially, the co-cultivation of T. matsutake mycelia and bacteria from shiro was introduced. In this study, bacteria were isolated from soil samples in Bonghwa-gun, and seven bacterial isolates (B22_7_B05, B22_7_B06, B22_7_B07, B22_7_B08, B22_7_B10, B22_7_B13, and B22_7_B14) promoted the growth of T. matsutake mycelia (147.48, 232.11, 266.72, 211.43, 175.17, 154.62, and 177.92%, respectively). Sequencing of the 16S rRNA region of the isolated bacteria was performed. B22_7_B05 and B22_7_B10 were identified as Bacillus toyonensis, B22_7_B06 and B22_7_B08 as Paenibacillus taichungensis, B22_7_B07 and B22_7_B14 as P. gorilla, and B22_7_B13 as P. odorifer. These bacterial isolates were associated with the shiro community and are expected to contribute to the cultivation of T. matsutake.

• Journal of Mushroom
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• v.11 no.1
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• pp.36-40
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• 2013

Armillaria gallica was ecologically surveyed to investigate its relationship with Gastrodia elata in cultivation areas of Korea in 2012. In the observation made around October 17 in the area of Namyangju (Gyeonggi), Sangju (Gyeongbuk) and Gimcheon (Gyeongbuk) in Korea, the fruit bodies of A. gallica were consistently found near the cultivation areas of Gastrodia elata across these cultivation areas. Since the temperature and rainfall have been considered as important factors of fruiting of A. gallica, we checked the temperature and rainfall around two weeks ago of mushroom fruiting. The average temperature of all cultivation areas was $9.9{\sim}17.5^{\circ}C$ (the lowest temp.: $3{\sim}23^{\circ}C$, the highest temp.: $15{\sim}26^{\circ}C$) and the annual rainfall was 0.5~1.0mm on October 10 which can be compared to 2.5~4.5mm on October 17. Fruiting bodies were generally developed at weedy field in which G. elata were cultivated five years ago. The field was inclined 15 degrees, east-facing and consists of loam. The fruit bodies were formed on both sides of a small ditch between the Gastrodia elata cultivation fields and neighboring fields, and the areas were common weeds and moisture at all times. The fruiting bodies were growing above the soil rather than oak wood. Gregarious, but also occurs to 1-5 fruiting bodies occur sporadically. Results observed in detail the growing areas, some fruit bodies were developed along the black and thick rhizomorph formation.