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Study on Rumen Cellulolytic Bacterial Attachment and Fermentation Dependent on Initial pH by cPCR (cPCR 기법을 이용한 초기배양 pH에 의한 반추위 섬유소 분해 박테리아의 부착 및 발효에 관한 연구)

  • Kim, M.S.;Sung, H.G.;Kim, H.J.;Lee, Sang-S.;Chang, J.S.;Ha, J.K.
    • Journal of Animal Science and Technology
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    • v.47 no.4
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    • pp.615-624
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    • 2005

  • The cPCR technique was used to monitor rumen fermentation and attachment of Fibrobacter succinogenes to cellulose at different pH in the in vitro culture medium. The target fragments of 16S rDNA(445 bp) were amplified from genomic DNA of F. succinogenes with specific primers and internal controls(205 bp) were constructed. Cell counts were estimated from the amounts of genomic DNA, which was calculated from cPCR results. F. succinogenes in pH 6.8 and 6.2 showed apparently higher attachment than in pH 5.8 during all incubation time. There were some difference between pH 6.8 and 6.2 in the degree of attachment, but the different was not significant (P>0.05). Cellulose degradation increased in process of incubation time and the increasing rate was higher when initial pH was higher. The pH in culture medium decreased regardless of initial pH in course of incubation time. After 24 h of incubation, medium pH was dropped by 0.24, 0.58 and 0.16 units from original medium pH 6.8, 6.2 and 5.8, respectively. More gas was produced at higher initial pH in the same manner as in cellulose degradation. In summery, Initial pH of rumen culture in vitro significantly influenced cellulose digestion, gas production, pH change and bacterial attachment. Especially, low pH(5.8) resulted in much lower bacterial attachment and fiber digestion compared to higher medium pH.

  • https://doi.org/10.5187/JAST.2005.47.4.615 인용 PDF KSCI

ON THE UNIQUENESS OF CERTAIN TYPE OF SHIFT POLYNOMIALS SHARING A SMALL FUNCTION

  • Saha, Biswajit
    • Korean Journal of Mathematics
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    • v.28 no.4
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    • pp.889-906
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    • 2020

  • In this article, we consider the uniqueness problem of the shift polynomials $f^n(z)(f^m(z)-1){\prod\limits_{j=1}^{s}}f(z+c_j)^{{\mu}_j}$ and $f^n(z)(f(z)-1)^m{\prod\limits_{j=1}^{s}}f(z+c_j)^{{\mu}_j}$, where f(z) is a transcendental entire function of finite order, cj (j = 1, 2, …, s) are distinct finite complex numbers and n(≥ 1), m(≥ 1), s and µj (j = 1, 2, …, s) are integers. With the concept of weakly weighted sharing and relaxed weighted sharing we obtain some results which extend and generalize some results due to P. Sahoo [Commun. Math. Stat. 3 (2015), 227-238].

  • https://doi.org/10.11568/kjm.2020.28.4.889 인용 PDF KSCI

Solid State Telescope on KAISTSAT-4

  • Y.H. Shin;Lee, J.J.;J.A. Hwang;M.P. McCarthy;Park, G.K. s
    • Bulletin of the Korean Space Science Society
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    • 2000.04a
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    • pp.39-39
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    • 2000

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