• Title/Summary/Keyword: Isoleucine

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Analysis of Amino Acid, Fatty Acid, and Vitamin in Korean Pine (Pinus koraiensis) Seeds (잣 종자(種子)의 아미노산(酸), 지방산(脂肪酸), 비타민 분석(分析))

  • Han, Sang Sup;Hwang, Byung Ho
    • Journal of Korean Society of Forest Science
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    • v.79 no.4
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    • pp.345-351
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    • 1990
  • The seeds of Korean pine, Pinus koraiensis, had been used as one of edible fruits for long time, but its chemical analysis of the nutrient components was extremely limited. The purpose of this study is to analyze the content of chemical components of Korean pine seeds. The results obtained are as follows : 1. In general analysis of Korean pine seeds, moisture is 4.4%, crude protein 18.3%, crude fat 67.3%, crude fiber 4.7%, ash 2.2%, and nitrogen-free extract 3.4%, respectively, 2. The Korean pine seed contained 18 different kinds of amino acid : lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, cysteic acid, and tryptophan. The glutamic acid is highest content among 18 kinds of amino acid. 3. The Korean pine seed contains all the essential amino acids such as arginine, histidine, lysine, threonine, valine, methionine, isoleucine, leucine, phenylalanine, and tryptophan. 4. The Korean pine seed contains 13 different kinds of fatty acid such as myristic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, 9-icosenoic acid, 9, 11-icosenoic acid, 8, 11, 14-icosatrienoic acid, and tn-o unknown substances. Also it contains all the essential fatty acids as linoleic acid and linolenic acid. The linoleic acid is highest content among 13 kinds of fatty acid. 5. The Korean pine seed contained 5 different kind., of vitamin such as vitamin A, vitamin $B_1$, vitamin $B_2$, vitamin E and niacin. The content of vitamin E is the largest among 5 kinds of vitamin.

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Quality Characteristics of Sulgidduk Prepared with Different Levels of Defatted Mealworm Powder (탈지 밀웜 분말 첨가량을 달리하여 제조한 설기떡의 품질특성)

  • Shin, Seung-Mee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.8
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    • pp.523-530
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    • 2019
  • This study was performed to develop Sulgidduk prepared with different levels (0, 3, 6, 9 and 12%) of defatted mealworm powder and to evaluate the characteristics of this Sulgidduk. We performed an assessment of color values, mechanical properties, general compositions and branched amino acids of Sulgidduk prepared with different levels of defatted mealworm powder. For the color values, the lightness(L) was decreased with increased amount of defatted mealworm powder, while the redness(a) and yellowness(b) were relatively increased. For the mechanical properties, the hardness and gumminess were significantly increased according to the increased amounts of defatted mealworm powder, but the chewiness, springiness and cohesiveness showed significant differences. For the sensory evaluation, the overall quality of Sulgidduk prepared with 6% defatted mealworm powder (MS-6) was higher as compared with that of the other Sulgidduk samples. For the general composition, the moisture and crude ash contents of MS-6 did not shown differences, while the crude protein was higher than those of the control, and the carbohydrate content of the control was higher than that of the MS-6. The contents of branched amino acids (valine, leucine and isoleucine) of MS-6 were higher than those of the control. Therefore, it was concluded that MS-6 could be a protein rich food as a good source of branched amino acids such valine, leucine and isoleucine.

Response of Achlya racemosa, A. proliferoides and Saprolegnia furcata to Sub-lethal Treatments of Amino Acids

  • Ali, Esam H.
    • Mycobiology
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    • v.31 no.2
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    • pp.86-94
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    • 2003
  • The effect of four sub-lethal concentrations(400, 800, 1,200 and 1,600 ${\mu}g/ml$) of three amino acids such as isoluecine, aspartic acid and phenylalanine on vegetative growth and sexual and asexual reproduction of Achlya racemosa, A. proliferoides and Saprolegnia furcata was investigated. The density of vegetative growth and diameters of vegetative colonies of species of the Oomycetes fungi decreased with rising the concentration of the applied amino acid. Vegetative hyphae of treated fungi almost appeared branched in case of S. furcata, thick in case of A. racemosa and distorted in case of A. proliferoides as compared with control. The different treatments with amino acids depressed both sporangial formation and discharge, which were dependent on the tested species of zoosporic fungi, the amino acid and its dosage. Phenylalanine was the most effective amino acid in inhibiting sporulation and S. furcata was the most sensitive fungal species. Aspartic acid and isoleucine stimulated germination of discharged spores through the formation of germlings. Gemmae formation by the three fungi was reduced at the low concentrations of amino acids and nearly missed at high concentrations. Sex organs(oogonia and antheridia) were affected partly; rudiment oogonia were observed at low concentrations(400 and 800 ${\mu}g/ml$) and disappeared at higher concentrations, whereas antheridial branch formation was stimulated as the fungi were treated with isoleucine and to some extent phenylalanine.

LC-MS-based metabolomic analysis of serum and livers from red ginseng-fed rats

  • Kim, Hyun-Jin;Cho, Chang-Won;Hwang, Jin-Taek;Son, Nari;Choi, Ji Hea;Shim, Gun-Sub;Han, Chan-Kyu
    • Journal of Ginseng Research
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    • v.37 no.3
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    • pp.371-378
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    • 2013
  • Serum and liver metabolites in rats fed red ginseng (RG) were analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The mass data were analyzed by partial least squares-discriminant analysis (PLS-DA) to discriminate between control and RG groups and identify metabolites contributing to this discrimination. The RG group was clearly separated from the control group on PLS-DA scores plot for serum samples, but not liver samples. The major metabolites contributing to the discrimination included lipid metabolites (lysophosphatidylcholine, acyl-carnitine, and sphingosine), isoleucine, nicotinamide, and corticosterone in the serum; the blood levels of all but isoleucine were reduced by RG administration. Not all metabolites were positively correlated with the health benefits of RG. However, the blood levels of lysophosphatidylcholine, which stimulate various diseases, and long-chain acylcarnitines and corticosterone, which activate the stress response, were reduced by RG, suggesting long-term RG might relieve stress and prevent physiological and biological problems.

A Comparison of the Essential Amino Acid Content and the Retention Rate by Chicken Part according to Different Cooking Methods

  • Kim, Honggyun;Do, Hyun Wook;Chung, Heajung
    • Food Science of Animal Resources
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    • v.37 no.5
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    • pp.626-634
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    • 2017
  • This study set out to identify the changes in the nutrient contents during the chicken cooking process as basic data for the establishment of a national health nutrition policy. Samples were produced using 3 chicken parts (wing, breast, and leg) and 7 cooking methods (boiling, pan-cooking, pan-frying, deep-frying, steaming, roasting, and microwaving), and the essential amino acid contents, principal components, and retention rates were analyzed. Weight loss was observed in all chicken parts with all cooking methods. The protein and essential amino acid contents of the chicken samples differed significantly according to the part and the cooking method (p<0.01). The protein and essential amino acid contents (g/100 g) of raw and cooked chicken parts showed ranges of 16.81-32.36 and 0.44-2.45, respectively. The principal component analysis (PCA) clearly demonstrated that the cooking methods and chicken parts produced similar trends for the essential amino acid contents. The retention rates of the chicken parts varied with the cooking methods, yielding a minimum value of 83% for isoleucine in a roasted wing, 91% for protein in a steamed breast, and 77% for isoleucine and lysine in a roasted leg. Therefore, the protein and amino acid contents of the roasted breast were higher than those of the other cooked chicken parts.

Analysis of the amino acid contents in the various parts of the forage corn 'Gwangpyungok'

  • Jung, Jeong Sung;Kim, Won Ho;Cho, Jin-Woong;Choi, Ki-Choon
    • Korean Journal of Agricultural Science
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    • v.45 no.3
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    • pp.379-384
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    • 2018
  • In this study, we analyzed the amino acid contents of corn to provide basic data for promoting the use of proteins in livestock. The present study was done to examine the amino acid contents of the corn 'Gwangpyungok' grown in a natural field at the National Institute of Animal Science, Cheonan province, Korea, in 2016. Gwangpyungok, which is Korean corn cultivar, was used as the sample to provide basic data for promoting the use of proteins in livestock by analyzing the amino acid contents of each part of corn as a breed that is adaptable to the environment of Korea. The asparagine acid content was the highest in the leaf blade among the parts of corn, and the glutamic acid content was the highest in the corn ear, stem, leaf sheath, corn bract and inflorescence. The essential amino acids in the corn ear, leaf blade and inflorescence revealed that their contents were in the following order: leucine > phenylalanine > valine > threonine > lysine > isoleucine, and in the inflorescences and stem, leaf sheath and corn bract, their contents were in following order: leucine > valine > phenylalanine > threonine > lysine > isoleucine. Therefore, further research on the nutritional aspects of forage must be performed because livestock growth is influenced by the nutritive value of the various parts of forage.

A Study on the Racemization of Amino acids and its Separation with GC, GC/MS and HPLC (아미노산의 광학이성화 및 GC, GC/MS, HPLC에 의한 광학이성질체의 분리에 관한 연구)

  • Rhee, Jae-Seong;Hong, Jong-Ki;Eo, Yun-Woo;Kim, Taek-Jae
    • Analytical Science and Technology
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    • v.7 no.1
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    • pp.41-52
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    • 1994
  • The importance of separation comes from demands on study for exact effect of synthetic drugs and the reactivity of enantiomer in biological system. Racemization rate was measured under the influence of heat, acid, UV-light, enzyme(trypsin) and 6N-HCl at $105^{\circ}C$ on alanine, threonine, isoleucine, lecuine, aspartic acid, methionine, glutamic acid, tyrosine. The method for the identification of overlapped amino acids with GC was developed from the close study of fragmentation pattern with mass spectrometry. With cyclodextrin bonded phase by HPLC, the separation of dansyl amino acid was tested for compartison.

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Quantitative Analysis of Free Amino Acids and Free Sugars in Steamed and Roasted Green Tea by HPLC (HPLC에 의한 증제와 볶음 녹차 중의 유리 아미노산과 유리당의 정량)

  • Ko, Young-Su;Lee, In-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.3
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    • pp.301-304
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    • 1985
  • Changes of free amino acids and free sugars in steamed and roasted green tea were determined after heat treatment at $110^{\circ}C$. Sixteen kinds of free amino acids and four kinds of free sugar were analyzed by HPLC. Free amino acids isolated were aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, histidine, lysine and arginine. Free sugars were sucrose, glucose, fructose, and raffinose. After the heat treatment, free amino acids and free sugars were decreased considerably.

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Assessment of Ethanol Fermentation with Rice Bran by Yeasts (米糠배지에서 酵母에 의한 에탄올 발효액의 평가)

  • 손경현;윤종수;성용분;이강표;김재철;이재흥
    • Microbiology and Biotechnology Letters
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    • v.20 no.1
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    • pp.85-90
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    • 1992
  • Rice bran was employed as a main medium component for ethanol fermentation by Saccharomyces species. Among the several strains of .Saccharomyces yeasts. S. cerevisiae IF0 2346 was selected as the hest strain in view of the interest in the production of ethanol and amino acids. It was found that S. cerevisiae IF0 2346 showed $3\times 10^8$cells/d and 4.7% (v/v) ethanol production after 72 hr cultivation. Although total amount of free amino acids was decreased from 1.099 mg/l to 829 mg/l during the fermentation, glutamic acid. histidine, and isoleucine were increased considerably. With the supplement of 5% glucose to the ferrnentation medium, both ethanol and amino acid production were increased up to 134% and 264%, respectively. compared to the control case. Glutamic. acid, leucine, alanine. phenylalanint:, and valine were the major amino acids in the fermentation broth.

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Analysis of amino acids in the liver of Hamster treated with Dimethylnitrosamine (Dimethylnitrosamine이 투여된 Hamster 간 속의 아미노산 분석)

  • Kim, Soo Gyung;Jung, Ha Seung;Park, Taek Kyu
    • Analytical Science and Technology
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    • v.7 no.2
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    • pp.165-171
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    • 1994
  • The effect of the chemical carcinogen dimetylnitrosamine(DMN) on the composition of amino acids of the liver in hamsters orally administered with DMN was studied by using the reversed phase high performance liquid chromatography technique. In the liver, the concentration of aspartic acid, glycine, glutamine, histidine, proline, tyrosine and leucine were increased ca. 2-fold of those observed in liver of control group, valine and tryptophan were increased ca. 3-fold, phenylalanine was markedly increased ca. 10-fold, whereas the concentration of threonine was decreased, serine, alanine, arginine, methionine, isoleucine and lysine were unchanged, respectively.

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