• Title/Summary/Keyword: Isolation rate

검색결과 629건 처리시간 0.029초

Distribution of Coagulase-Negative Staphylococci and Antibiotic Resistance

  • Park, Heechul;Park, Sung-Bae;Kim, Junseong;Kim, Sunghyun
    • 대한의생명과학회지
    • /
    • 제27권2호
    • /
    • pp.45-50
    • /
    • 2021
  • Coagulase-negative staphylococci (CoNS) are a typical group of microorganisms, and the recent advances in laboratory technology and medicine has dramatically modified their significance in medical practice. CoNS, which were previously classified as normal bacterial flora, have recently been reported to be associated with serious infectious diseases, such as surgical wound infection or periprosthetic joint infection. Representative CoNS include Staphylococcus epidermidis, S. haemolyticus, and S. saprophyticus, which are known to cause serious problems in biomaterial-based and prosthetic device infections, as well as to cause simple urinary tract infections in sexually active women. Over the last decade, the clinical isolation rate of CoNS has been increasing, and antibiotic resistance has also been occurring. This review aimed to investigate the incidence of CoNS infection and to use the results as basic data for the management of CoNS, with a focus on the isolation rate and antibiotic resistance in clinical surgery.

시술자와 방습법에 따른 치면열구전색제의 유지에 관한 연구 (EVALUATION OF PIT AND FISSURE SEALANTS RETENTION BETWEEN DIFFERENT OPERATORS USING DIFFERENT ISOLATION TECHNIQUES)

  • 조재용;김정욱;장기택;김종철;한세현;이상훈
    • 대한소아치과학회지
    • /
    • 제30권3호
    • /
    • pp.415-422
    • /
    • 2003
  • 치면열구전색제의 예방효과는 전색제의 유지와 매우 밀접한 상관관계가 있으며 시술시의 환경과 시술 방법, 개개 환자의 구강 위생이 유지에 영향을 미치는 것으로 알려져 있다. 현재 우리나라에서는 대규모로 '치아홈메우기 사업'이 진행 중에 있지만 시술 여건이나 시술자에 따라 여러 가지 방법의 방습법과 치면 세마가 행해지고 있는 실정이다. 본 연구의 목적은 치면열구전색제의 시술 시 소아치과의사, 일반치과의사, 치과위생사 등 시술자에 따른 유지율 차이와 러버댐과 코튼롤(cotton roll) 방습법 등 격리 방법에 따른 유지율의 차이를 알아보고 실패가 일어나는 치아의 특정 부위를 알아보기 위한 것이다. 6세-11세인 119명의 아동(남자 64명, 여자 55명)에서 상하악 제1대구치에 시술된 279개의 치면열구전색제를 대상으로 1년 후의 유지율을 조사하였다. 이 아동들은 평균 연령이 $8.59{\pm}0.772$세였으며 각각 러버댐을 사용한 소아치과의사가 시술한 1군, 러버댐을 사용한 일반치과의사가 시술한 2군, 코튼롤(cotton roll)을 사용한 치과위생사가 시술한 3군으로 나누었다. 1년 후의 치면열구전색제의 유지율을 조사하여 다음과 같은 결론을 얻었다. 1. 1년 후의 치면열구전색제 유지율은 러버댐을 사용한 소아치과의사가 시술한 1군이 90.8%, 러버댐을 사용한 일반치과 의사가 시술한 2군은 85.0%, 코튼롤을 사용한 치과위생사가 시술한 3군은 64.7%였으며 1군과 2군간에는 통계적인 유의차가 없었으나(P>0.05) 1,2군과 3군 간에는 통계적인 유의차가 있었다(P<0.05). 2. 상악과 하악의 유지율 비교에서 1군과 2군은 차이가 없었으나(P>0.05) 3군은 차이가 있었다(P<0.05). 3. 실패부위 분석에서 교합면의 실패는 58.3%, 협/설면구는 41.7%를 차지하였다.

  • PDF

닭의 폐(肺)에서 병원성(病原性) Aspergilli 의 분리(分離)와 탄소원(炭素源)및 질소원(窒素源)에 따른 배양조건(培養條件)에 관(關)하여 (Studies on the Incubation Condition according to Carbon Source, Nitrogen Source and Isolation of Pathogenic Aspergilli from Lung of Domestic Fowls)

  • 이배함;이경배
    • 한국균학회지
    • /
    • 제1권2호
    • /
    • pp.25-30
    • /
    • 1973
  • 1973년(年) 7월(月) 서울특별시내(特別市內) Smoke통닭 공장(工場)에서 얻은 이병닭 폐(肺)에서 Aspergilli를 분리(分離)할 수 있었고 또 C-source 및 N-source의 배양조건(培養條件)을 실험(實驗)한 결과(結果) 다음과 같은 결론(結論)을 얻었다. 1. Aspergillus fumigatus group에 속(屬)한 1 strain을 분리(分離) 하였다. 2. 분리용(分離用) 배지(培地)로서는 Czapek agar에 누에, 메주를 4g씩 첨가(添加)한 것이 과거 Austuwick(1962)가 보고한 2% malt extract agar medium보다 양호하였다. 3. 배양(培養)에 있어서 C-source는 종류(種類)와 양(量)에 따라 생장율과 sporulation의 차(差)가 많았고 20%에서 생장율이 떨어겼다. N-source에서는 종류(種類)에 따라 생장율의 차가 생기나 양(量)에 따라서는 생장율의 차(差)가 거의없고 다만 mycelium과 sporulation에서 차(差)가 생겼다.

  • PDF

Ethylene Glycol 분해균주의 순수분리.동전 및 배양특성 (Pure Isolation, Identification and Culture Characteristics of Ethylene Glycol Degrading Bacteria)

  • 류원률;최장승;주무환
    • KSBB Journal
    • /
    • 제13권5호
    • /
    • pp.518-523
    • /
    • 1998
  • A large quantity of ethylene glycol(EG) is remained in the effluent after pretreating polyester weight-loss wastewater physicochemically in the fist stage and must be treated biologically in the second stage. Therefore, an excellent EG-utilizing bacteria strain was isolated from the natural system and the optimal culture conditions of the strain were investigated. The optimal culture conditions of temperature, pH, and nitrogen source were found to be 35$^{\circ}C$, 7.5 and ammonium chloride, respectively, when CODCr removal efficiency was more than 90%. The growth of stains and EG removal efficiency was slightly improved by adding elements such as niacin and biotin. With increasing inoculation size in a batch culture, the removal efficiency of EG was conspicuously increased. Growth rate was inhibited when the initial concentration of EG was more then 30g/L. The strain was identified as Pseudomonas sp. based on morphological and biological characteristics and named as Pseudomonas sp. EG1.

  • PDF

노변식품에서 분리한 Campylobacter jejuni의 특성에 관한 연구 (Study on Characterizations of Campylobacter jejuni Isolated from Street Vended Foods)

  • 빈재훈;차인호;김용환;하상태;김경숙;권혁동;이채남
    • 한국식품위생안전성학회지
    • /
    • 제9권3호
    • /
    • pp.117-121
    • /
    • 1994
  • On the purpose of epidemiological survey related to foods poisoning, a total of 316 specimens collected from street vended foods in Pusan area were examined for the presence of Campylobacter Jejuni(C. jejuni) and also the isolation rates, biochemical properties and antibiotic susceptibility were investigated. Of the 316 specimens, 13 strain(4.1%) of Campylobacter jejuni were isolated. Isolation rate for each food was 37.5% in gizzard, 4.1% in Korean sausage and 3.9% in kimpop. Isolated C. jejuni were grouped as biotype I(84.6%), II(7.7%) and IV(7.7%). C. jejuni isolated from street vended foods were resistant to cephalothin(100%), penicillin(84.6%) and erythromycin(76.9%), but sensitive to gentamicin(92.3%), kanamycin(84.7%) and chloramphenicol(77.0%).

  • PDF

서울에서의 인플루엔자 B바이러스 유행감시, 1985-1986 (Laboratory Surveillance of Influenza B Epidemic in Seoul During the Winter of 1985-1986)

  • 박기덕;조양벽;김영선;백승복;금동혁;신미자
    • 대한미생물학회지
    • /
    • 제22권1호
    • /
    • pp.9-13
    • /
    • 1987
  • During 31 days (5 week) from December 9th 1985 to January 8th 1986, Influenza B epidemic were observed in Seoul city. Epidemic peak was shown at 52nd week in 1985. The Epidemic associated viruses were determined as B/yamanish/510/84 and B/Ann Arbor/1/86-like strain. From total of 124 specimens of influenza-like illness children virus isolation rate were average 4.0%, much lower than that of influenza A virus isolation experience. In the epidemic influenza B infection were predominantly involved in school age children.

  • PDF

도체표면의 분변오염과 Verotoxin 생성 Escherichia coli O157:H7 분리에 관한 연구 (Isolation of Verocytotoxin Producing Escherichia coli O157:H7 Due to Fcal Contamination on Carcass Surfaces)

  • 홍종해;고주언
    • 한국식품위생안전성학회지
    • /
    • 제12권1호
    • /
    • pp.78-82
    • /
    • 1997
  • Surface swab samples from beef (188), pork (240) and chicken (95) carcasses were collected from slaughterhouse in Kangwon and Kyunggi areas from March through July 1996. The samples were examined on the level of E. coli biotype I relevant to fecal contamination due to unsanitary processing control and the existence of verocytotoxin-producing E. coli (VTEC). E. coli biotype I were confirmed from 38.8% of beef, 40.0% of pork, and 69.5% of chicken carcasses. Little variation was noted among three sampling points; rump, flank and neck of beef, ham, belly and jowls of pork. coli O157:H7 was only confirmed from 2 of 188 beef carcasses. E. coli biotype I. All the isolated E. coli O157 showed positive for vero cell cytotoxicity test. Isolation rate of E. coli O157 in summer was higher than in spring. In case of pork and chicken carcasses, E. coli O157 was isolated in summer only.

  • PDF

한우 초유로부터 Lactoferrin의 분리.정제 (Isolation and Purification of Lactoferrin from Korean Native Cow's Colostrum)

  • 양희진;하월규;양동훈;박기문;이수원
    • 한국축산식품학회지
    • /
    • 제20권2호
    • /
    • pp.125-132
    • /
    • 2000
  • Lactoferrin was isolated from the colostrum of Korean native cow by using several purification steps such as batch extraction, ion exchange chromatography, gel filtration chromatography and affinity chromatography. Other whey protein components that having similar molecular weight and affinity to lactoferrin were gradually removed from crude Korean native cow's lactoferrin during the purification steps. The amount of lactoferrin collected from a liter of Korean native cow's colostrum was 65mg and the recovery rate was 29.4%. The molecular weight of the purified Korean native cow's lactoferrin was estimated approximately 81,000dalton.

  • PDF

Isolation of Fucosterol from Pelvetia siliquosa by High-speed Countercurrent Chromatography

  • Hwang, Seung Hwan;Jang, Jai Man;Lim, Soon Sung
    • Fisheries and Aquatic Sciences
    • /
    • 제15권3호
    • /
    • pp.191-195
    • /
    • 2012
  • We report here the use of high-speed countercurrent chromatography (HSCCC) in the preparative isolation and purification of the bioactive component, fucosterol, from Pelvetia siliquosa. A crude extract was obtained by ultrasonic extraction of powdered P. siliquosa using methylene chloride and was then subjected to separation and purification by HSCCC, coupled with evaporative light-scattering detection. Preparative HSCCC was performed successfully using a two-phase solvent system, n-heptane:methanol (3:2, v/v), to obtain 10.96 mg fucosterol with 96.8% purity from 50 mg of crude extract; the recovery rate was approximately 90.5%.

High-Performance Reversible Data Hiding with Overflow/Underflow Avoidance

  • Yang, Ching-Yu;Hu, Wu-Chih
    • ETRI Journal
    • /
    • 제33권4호
    • /
    • pp.580-588
    • /
    • 2011
  • This paper proposes reversible data hiding using minimum/maximum preserved overflow/underflow avoidance (MMPOUA). The proposed MMPOUA algorithm consists of three main steps. These steps include the minimum (or maximum) pixel fixing, pixel squeezing, and pixel isolation. The aims of pixel fixing are to keep the minimum (or maximum) pixel of a host block unchanged and prevent the occurrence of overflow/underflow. Both the pixel squeezing and pixel isolation supply hiding storage while keeping the amount of distortion low. The proposed method can avoid (or significantly reduce) the overhead bits used to overcome overflow/underflow issues. At an embedding rate of 0.15 bpp, the proposed algorithm can achieve a PSNR value of 48.52 dB, which outperforms several existing reversible data hiding schemes. Furthermore, the algorithm performed well in a variety of images, including those in which other algorithms had difficulty obtaining good hiding storage with high perceived quality.