• Journal of Embryo Transfer
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• v.33 no.4
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• pp.327-336
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• 2018

To date, there are no protocols optimized to the effective separation of spermatogonial stem cells (SSCs) from testicular cells derived from mouse testes, thus hindering studies based on mouse SSCs. In this study, we aimed to determine the most efficient purification method for the isolation of SSCs from mouse testes among previously described techniques. Isolation of SSCs from testicular cells derived from mouse testes was conducted using four different techniques: differential plating (DP), magnetic-activated cell sorting (MACS) post-DP, MACS, and positive and negative selection double MACS. DP was performed for 1, 2, 4, 8, or 16 h, and MACS was performed using EpCAM ($MACS^{EpCAM}$), Thy1 ($MACS^{Thy1}$), or GFR ${\alpha}1$ ($MACS^{GFR{\alpha}1}$) antibodies. The purification efficiency of each method was analyzed by measuring the percentage of cells that stained positively for alkaline phosphatase. DP for 8 h, $MACS^{Thy1}$ post-DP for 8 h, $MACS^{GFR{\alpha}1}$, positive selection double $MACS^{GFR{\alpha}1/EpCAM}$, and negative selection double $MACS^{GFR{\alpha}1/{\alpha}-SMA}$ were identified as the optimal protocols for isolation of SSCs from mouse testicular cells. Comparison of the purification efficiencies of the optimized isolation protocols showed that, numerically, the highest purification efficiency was obtained using $MACS^{GFR{\alpha}1}$. Overall, our results indicate that $MACS^{GFR{\alpha}1}$ is an appropriate purification technique for the isolation of SSCs from mouse testicular cells.

• Journal of Industrial Convergence
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• v.20 no.3
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• pp.47-54
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• 2022

This study aimed to identify the mediating effect of social support on the relationship between social isolation and depression in nurses. This study was conducted with 135 registered nurses in three COVID-19 specialized hospitals. Data were analyzed with IBM SPSS Statistics 25.0 and using descriptive statistics, Pearson correlation coefficient, and mediation analysis. The mediating effect was analyzed using PROCESS macro for SPSS 4.0 program with bootstrapping method. Social isolation directly influenced social support (𝛽=-.62, p<.001) and depression (𝛽=.49, p<.001). Social support directly influenced depression (𝛽=-.19, p=.028). Social support has a mediating effect between social isolation and depression (indirect effect=.12, 95% CI=0.02~0.29). To managed depression, intervention are needed to reduce social isolation. Therefore, it is necessary to develop strategies that enhance social support to relieve depression in nurses perceived social isolation.

• Structural Engineering and Mechanics
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• v.83 no.5
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• pp.627-644
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• 2022

In past major earthquakes (1994 Northridge, 1995 Kobe, Chi-Chi 1999, Kocaeli 1999), significant damages occurred in the liquid storage tanks. The basic failure patterns were observed to be the buckling of the tank wall and uplift of the anchorage system. The damages in the industrial facilities and nuclear power plants have caused the spread of toxic substances to the environment and significant fires. Seismic isolation can be used in liquid storage tanks to decouple the structure and decrease the structural demand in the superstructure in case of ground shaking. Previous studies on the use of seismic isolation systems on liquid storage tanks show that an isolation system reduces the impulsive response but might slightly increase the convective one. There is still a lack of understanding of the seismic response of seismically isolated liquid storage tanks considering the fluid-structure interaction. In this study, one broad tank, one medium tank, and one slender tank are selected and designed. Two- and three-dimensional elastomeric bearings are used as seismic isolation systems. The seismic performance of the tanks is then investigated through nonlinear dynamic time-history analyses. The effectiveness of each seismic isolation system on tanks' performance was investigated. Isolator tension forces, modal analysis results, hydrodynamic stresses, strains, sloshing heights and base shear forces of the tanks are compared. The results show that the total base shear is lower in 3D-isolators compared to 2D-isolators. Even though the tank wall stresses, and strains are slightly higher in 3D-isolators, they are more efficient to prevent the tension problem.

• 한국노년학
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• v.30 no.3
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• pp.895-910
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• 2010

This study examined the mediating effect of social isolation on the life satisfaction among lowincome older adults. The purpose of this study was to find out isolated older adults' social isolation rate and mediating effect of sense of community by community dwelling older adults with loweconomic status. This study used the Gijang Welfare Network study(2009). The sample consisted 256 older adults 65 and older residing in Busan(Gijang). As statistical method, correlation analysis and structural equation model used in this paper. The results of this study suggested some practical implications in reducing social isolation for older adults with low economic status. We need to emphasize social relations and social participations as well as outreach program for high-risk elderly of social isolation. In addition, Findings suggested that community based interventions should be provided to protect older people, especially poor elderly from social isolation.

• Earthquakes and Structures
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• v.27 no.2
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• pp.143-154
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• 2024

Friction pendulums typically suffer from poor uplift-restraining. To improve the uplift-restraining and enhance the energy dissipation capacity, this article proposed a composite isolation device based on electromagnetic forces. The device was constructed based on a remote control system to achieve semi-active control of the composite isolation device. This article introduces the theory and design of an electromagnetic chuck-friction pendulum system (ECFPS) and derives the theoretical equation for the ECFPS based on Maxwell's electromagnetic attraction equation to construct the proposed model. By conducting 1:3 scale tests on the electromagnetic device, the gaps between the practical, theoretical, and simulation results were analyzed, and the accuracy and effectiveness of the theoretical equation for the ECFPS were investigated. The hysteresis and uplift-restraining performance of ECFPS were analyzed by adjusting the displacement amplitude, vertical load, and input current of the simulation model. The data obtained from the scale test were consistent with the theoretical and simulated data. Notably, the hysteresis area of the ECFPS was 35.11% larger than that of a conventional friction pendulum. Lastly, a six-story planar frame structure was established through SAP2000 for a time history analysis. The isolation performances of ECFPS and FPS were compared. The results revealed that, under horizontal seismic action, the horizontal seismic response of the bottom layer of the ECFPS isolation structure is greater than that of the FPS, the horizontal vibration response of the top layer of the ECFPS isolation structure is smaller than that of the FPS, and the axial force at the bottom of the columns of the ECFPS isolation structure is smaller than that of the FPS isolation structure. Therefore, the reliable uplift-restraining performance is facilitated by the electromagnetic force generated by the device.

• Proceedings of the Materials Research Society of Korea Conference
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• 1992.05a
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• pp.61-61
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• 1992

• Journal of The Korea Institute of Healthcare Architecture
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• v.24 no.1
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• pp.51-58
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• 2018

Purpose: In this study, through the comparison of the pressure fluctuation and air exchange volume in negative isolation room according to the type of the door and door opening/closing speeds, which is one of the main factors causing the cross contamination of the negative pressure isolation room, establishes standard operating procedures to prevent cross contamination in high risk infectious diseases and isolation room design. Methods: In this study, the air flow each of the room is analyzed using ANASYS CFX CODE for flow analysis. In addition, the grid configuration of the door is constructed by applying Immersed Solid Methods. Results: The pressure fluctuation due to the opening and closing of the hinged door was very large when the moment of the hinged door opened and closed. Especially, at the moment when the door is closed, a pressure reversal phenomenon occurs in which the pressure in the isolation room is larger than the pressure in the anteroom. On the other hand, the pressure fluctuation due to the opening and closing of the sliding door appeared only when the door was closed, but the pressure reversal phenomenon not occurred at the moment when the sliding door was closed, unlike the hinged door. As the opening and closing speed of the hinged door increases, the air exchange volume is increased. However, as the opening and closing speed of the sliding door is decreased, the air exchange volume is increased. Implications: According to the results of this study, it can be concluded that the pressure fluctuation due to the opening and closing of the hinged door is greater than the pressure fluctuation due to the opening and closing of the sliding door. In addition, it can be confirmed that the pressure reversal phenomenon, which may cause to reduce the containment effect in negative pressure isolation room, is caused by the closing of the hinged door. Therefore, it is recommended to install a sliding door to maintain a stable differential pressure in the negative isolation room. Also, as the opening and closing speed of the hinged door is slower and the opening and closing speed of the sliding door is faster, the possibility of cross contamination of the room can be reduced. It is therefore necessary to establish standard operating procedures for negative isolation room for door opening and closing speeds.

• The Journal of the Korean Society for Microbiology
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• v.9 no.1
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• pp.13-18
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• 1974

Blood is one of the most important clinical specimens for the isolation of bacteria. A rapid isolation and a high isolation rate of bacteria are very important in blood culture because bacteremic patients are mostly in grave condition. Various blood culture media which support growth of most fastidious bacteria are available commercially. However, growth of bacteria are frequently delayed because of antibacterial activity of blood. Sodium polyanethol sulfonate(Liquoid) has been reported to inactivate the antibacterial substance and disrupt phagocytic cells. The beneficial effect of SPS is well recognized in the isolation of gram-positive bacteria. However, the effect does not seem to be prominent for gram-negative bacilli isolation mainly due to the rapidity of their growth. It has been experienced with Sal. typhi that the growth is much slower than that of other gram-negative bacilli. For the rapid growth of the organism, use of bile broth has been recommended. Although Sal. typhi is the most frequently isolated organism at present, about one half of total isolates are other organisms and, in case bile broth is used, other media which support growth of these organisms should be used together. Fluid thioglycollate medium(FTM) which is always used in blood culture to isolate anaerobes is inferior to brain heart infusion(BHI) for the isolation of aerobes. This study was done to determine the effect of SPS on the isolation of Sal. typhi from blood. During the Sep. 1973 to Sep. 1974 study period, 2460 blood cultures were made from the Severance hospital patients: BHI and FTM sets 1431 specimens, BHI with SPS(0.05%) and FTM sets 396 specimens, BHI and FTM with SPS sets 359 specimens, BHI and BHI with SPS sets 274 specimens. Mean incubation time required for the macroscopic detection of growth of Sal. typhi were 3.5 days on BHI and 2.7 days on BHI with SPS. The 0.8 day difference was statistically significant. On FTM the mean incubation time was 3.8 days while it was 2.9 days on FTM with SPS. The 0.9 day difference was statistically significant. The result on BHI with and without SPS sets showed faster growth on BRI with SPS in 7 specimens and slower growth in one specimen and the remaining 12 showed growth at the same time. These specimens had mean incubation time of 3.2 days on BHI and 2.3 days on BHI with SPS. The 0.9 day difference was statistically significant. This study indicates beneficial effect of SPS for the rapid isolation of Sal. typhi from clinical blood specimens.

• Korean Journal of Veterinary Service
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• v.22 no.3
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• pp.221-237
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• 1999

To investigate the specificity of rapid slide agglutination test for pullorum-gallinarum diseases and to obtain a basic data for avian salmonellosis control, salmonella isolation was peformed for the layer chickens positively reacted in pullonlm-typhoid agglutination test. The biochemical, serological and antimicrobial properties of the isolates were examined. The results obtained through this study were summarized as follows; 1. Of 2,384 chickens tested by the agglutination test, 606 chickens (25.4%) were positive reactors. 154 of 606 reactors and 49 of the non-reacting chickens were investigated for salmonella isolation, resulting in isolation of 68 strains of salmonellae from 27 chickens. 2. By organs, the isolation frequency from liver, cecum, spleen, ovary and gall bladder showed 8.9% (18 strains), 8.9% (18 strains), 7.4% (15 strains), 4.4% (9 strains) and 3.9% (8 strains), respectively. 3. By culture medium the combination of selenite broth and MacConkey agar revealed the highest isolation rate and the enrichment culture by delayed secondary enrichment culture method was found the most effective for salmonella isolation. 4. The serotypes of 68 salmonella isolates were identified as 3 strains of S pullorum, 24 strains of S gallinarum, 15 strains of S typhimurium, 8 strains of S enteritidis, 7 strains of S paratyphi A, 5 strains of S typhimurium and 6 strains of the other salmonellae. 5. The serotypes of 8 salmonella strains isolated from 49 chickens non-reacting in pullorum-typhoid agglutination test were identified as 3 strains of S typhimurium and 5 strains of S infantis. 6. When 24 chickens of which 68 strains of salmonellae isolated were examined by microplate agglutination test, the average antibody titer for pullorum antigen was $2^{5.25}$. The chickens at antibody titer between $2^3$ and $2^5$ showed the higher frequency of isolation as compared with the chickens at the other titers. 7. When salmonella isolates were tested the antimicrobial drug sensitivity by disk diffusion method, S paratyphi A were highly sensitive by 100% to ATM and GM, S typhimurium, by 88% to AM, CIP, IMP and TN, S infantis, by 100% to AM, CRO, ENR and PIP, S enteritidis,by 100% to IMP and PIP, S pullorum, by 100% to ATM, CRO, ENR and PIP and S gallinarum, by 92% to CRO, CIP and PIP.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.9
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• pp.1642-1649
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• 2007

The lumped element isolators with temperature compensation magnetic circuits were designed in this paper. Isolators for mobile repeater at each band of K-PCS, GSM1900, W-CDMA, WiBro were fabricated by using optimum parameters of EM simulation, Form the results of this study, first of all, insertion loss at K-PCS band was below 0.2 dB and return loss was over 25dB. The bandwidth in isolation 23dB was found 30 MHz. Secondly, the figures of both losses at GSM1900 were 0.25dB and 23dB, respectively. The measured bandwidth in isolation 30dB was 60MHz. Thirdly, the losses at W-CDMA band were 0.15dB and 25dB. The bandwidth in isolation 24.8dB was found 60Mhz. Finally, the figures of both losses at Wibro band were 0.25dB and 23dB, respectively. The measured bandwidth in isolation 22.1dB was 100Mhz. In addition, the results of measured IMD were shown from 76.4dBc to 80.1dBc.