• 한국환경보건학회지
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• 제5권1호
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• pp.51-57
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• 1978

Antibiotics susceptibilities of Staphylococci were tested to get useful data for prevention food contamination and treatment of food poisoning. Tested were colony counts, isolation of Staphylococci, identification for the Staphylococcus aureus and resistant patterns to antimcrobial agents. The colonies and Staphylococci isolated from the atmosphere of 37 home kitchens in the morning (07:00-08:00) on Nov. 19, 1977 in Busan. The findings are as follows: 1. The average of colony counts was shown to be 9.6 ranging from 47 to 1 at mean temperature of 18$\circ$C (the highest 21$\circ$C, the lowest 15$\circ$C) 2. Out of total 37 kitchens, 18 Staphylococci were isolated from 15 kitchens with 40.5 per cent, and 4 Staphylococcus aureus were identified with 22.2 per cent from 18 Staphylococci. 3. Isolated Staphylococci were resistant to penicillin(100%), dihydrostreptomycin(93.3%), tetracycline (93.3%) and oleandomycin(66.6%), while the strains were sensitive to sulfonamide(88.6%) and colistin(83.3%). 4. Staphylococcus aureus identified were resistant to dihydrostreptomycin, penicillin and tetracycline, while the strains were sensitive to sulfonamide and colistin.

• 미생물학회지
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• 제50권1호
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• pp.8-14
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• 2014

본 연구는 home-made 요구르트에서 분리한 Lactobacillus casei SK-7과 시중에서 판매되고 있는 요구르트에서 분리한 Lactobacillus bulgaricus YK-11의 기능성을 비교 분석하기 위해 실시하였다. 먼저 분리세균인 SK-7과 YK-11의 생리화학적 특성조사를 진행하였다. 이들 균주는 16S rRNA 염기서열을 이용한 계통유전학적 분석방법으로 동정하여, 각각 L. casei SK-7와 L. bulgaricus YK-11로 명명하였다. 16S rRNA 염기서열을 바탕으로 SK-7과 YK-11의 유전적 계통수를 작성하였다. 72시간 동안 SK-7과 YK-11 배양에서 lactic acid와 acetic acid의 생산, 그리고 pH 변화를 조사하였다. 배양기간 동안 L. casei SK-7과 L. bulgaricus YK-11의 몇가지 기능성을 조사하였다. L. casei SK-7과 L. bulgaricus YK-11 배양상등액은 주어진 nitrite를 각각 93.9%와 88.2% 소거하였다. SK-7 and YK-11 배양상등액의 항산화능은 각각 62.6%와 54.9%였으며, ${\beta}$-galactosidase 활성은 14.9 units/mg과 13.1 units/mg로 측정되었다. 항미생물능력은 SK-7과 YK-11 배양의 20배 배양농축액으로 조사되었으며, SK-7은 이 조사에 사용된 모든 미생물에서 명백한 것으로 관찰되었으나, YK-11에서는 관찰되지 않았다. 부가적인 기능성 연구가 이루어져야 되겠지만, 본 연구에서는 home-made 요구르트에서 분리된 젖산균의 기능성은 시판요구르트에서의 기능성과 비교하여 우수한 것으로 조사되었다.

• 한국균학회지
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• 제9권3호
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• pp.147-151
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• 1981

During the period of June 15, 1978 to February 28, 1979 fungi were isolated from 101 public and home telephones in 6 different cities and classified. Total 203 isolates were obtained and classified into 8 genera and 6 species, however 44 of the isolates were unidentified. Penicillium spp. were frequently found. Forty isolates were derivated from coffee shops, 51 from street public telephones, 35 from stores, 26 from offices and 19 from homes. Also 27 were from Seoul, 30 from Daegu, 46 from Gunsan, 82 from Chunju and 18 from Jaeju. Twenty-seven were isolated in summer and 174 in winter. From the above observations, it is concluded that the transimitters are heavily colonized with various species of fungi and some of them are pathogens. The rates of colonization appeared to be various by locations and seasons.

• 한국미생물·생명공학회지
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• 제25권2호
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• pp.224-228
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• 1997

This studies were carried out to investgate the effects of omija (Schizandra chinensis) extracts(OE) on the growth of lactic acid bacteria (LAB, HYO-1 and HYO-2) isolated from home-made kimchi. The growth of isolated LAB was inhibited apparently in modified MRS broth containing 1% or 2% of OE. The growth of isolated LAB was inhibited by pH control with HCI to 4.3 or 3.7, the same pH of modified MRS broth containing 1% or 2% of OE. The strong growth inhibitory properties against isolated LAB were shown by fumaric and itaconic acid among various organic acids of Schizandra chinensis, such as citric acid, malic acid, lactic acid, fumaric acid, itaconic acid and acetic acid. The isolated LAB were indentified as Lactobacillus plantarum.

• Journal of Nutrition and Health
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• 제16권2호
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• pp.81-88
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• 1983

This study was performed to investigate the influence of the amino acid composition of diet and environment on RNA, protein content in brain and learning ability in rats. Forty-two Sprague-Dawley male rats were divided into six groups according to type of diet, casein, soybean meal, or corn gluten and rearing condition, isolated or enriched. They were fed foods ad libitum for 6 weeks. A water maze was used to test behavioral performance for 3 weeks from 4th week. The rats were sacrificed at 6th week and their whole brains were taken and frozen for assay of RNA and protein. The results were summarized as follows : 1) The body weight gain for the experimental periods of corn gluten group was significantly lower than the casein and the soybean meal group. 2) The brain weight of the corn gluten group was significantly lower than the casein and the soybean meal group and the environmental enrichment slightly increased it among rats fed the corn gluten diet. 3) The total RNA contents were the greatest in the environmentally enriched casein group. The brain protein contents of the isolated corngluten group was the smallest. However, the contents of the enriched corn gluten group were similar to those of the others. 4) In the water maze test, the isolated corngluten group spent significantly more time than the others. Environmental enrichment could decrease time to perform the task of the maze.

• 성인간호학회지
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• 제19권2호
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• pp.329-337
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• 2007

Purpose: Increasing numbers of Koreans have immigrated to the United States since the late 1960s. The first generation of Korean immigrants or their parents become old and institutionalized in American nursing home setting. Although the Korean elders would experience many cultural differences in the nursing home, no study to date has investigated their everyday lives on how they live through their later lives within a different cultural environment from their own. Methods: Using ethnographic methodology, the purpose of this paper was to illustrate Korean residents' experiences and daily lives in a nursing home located in an east coastal city in the U.S. Participant observation, filed notes, semi-structured interviews were utilized by means of data collection. Eighteen Korean residents were observed, and five of them and two nurses participated in informal qualitative interviews. Results: The overriding theme from the findings is "thrown in a different world." Three sub-themes include "constant struggles in making themselves understood", "dealing with culturally inappropriate nursing care," and "maintaining their own ways of life". Conclusions: The discovered themes reflect culturally isolated lives of the participants and open a venue for designing a culturally congruent nursing care for Korean elders living in the U.S. nursing homes.

• 약학회지
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• 제53권4호
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• pp.194-200
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• 2009

Community-acquired antimicrobial resistant bacteria are an emerging problem whole world-wide. Generally, Hands are main mediator of pathogen transmission as compared with other body parts. So, the purpose of this research was to investigate the microbiological quality and antimicrobial susceptibilities of samples which were collected from the door knob and surface in public- and home-facilities, and also hand. Of total 191 samples, 71 samples (37%) were shown to be of high-level total bacterial count (>$10^8\;CFU/cm^2$). And presence of Staphylococcus and Enteric bacilli was observed in 61 samples (32%) and 76 samples (40%), respectively. Antimicrobial susceptibilities of staphylococcal isolates from the samples were tested for six different antimicrobial agents, which are in wide spread clinical use in Korea, as well as four new antimicrobials, daptomycin, linezolid, quinupristin/dalfopristin and tigecycline. Among staphylococcal isolates, antimicrobial resistance were observed in oxacillin (n=6), mupirocin (n=7), vancomycin (n=1), quinupristin/dalfopristin (n=2) and gentamicin (n=5). Fortunately, all the isolates were susceptible to new antimicrobial such as daptomycin, linezolid and tigecycline. Furthermore 4 Enterococcus faecalis were isolated from four hand samples, and all these isolates exhibited multidrug resistance to four different antimicrobial (oxacillin, quinupristin/dalfopristin, mupirocin, gentamicin). Also, 5 Escherichia coli were isolated from surface in home (n=3), door knob in public facilities (n=1) and hand (n=1). Escherichia coli isolated from hand was high-level resistant to tigecycline ($128{\mu}g$/ml) and gentamicin ($64{\mu}g$/ml).

• Archives of Pharmacal Research
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• 제8권3호
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• pp.133-137
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• 1985

The aglycones of saponins isolated from Melandrium firmum were identified as gypsogenin, gypsogenic acid and quillaic acid.

• Restorative Dentistry and Endodontics
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• 제6권1호
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• pp.93-103
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• 1980

Streptococcus mutans were isolated from dental plaques of carious lesions of 4 patients on mitis-salivarius agar medium. Three patients known to harbor S. mutans in their dental plaques. Identification of the isolated S. mutans was established by colonial morphology on mitis-salivarius agar medium, the fermentation of mannitol and sorbitol, and confirmed by agglutinating reaction with home made anti-S. mutans NCTC 10449 (serotype c) antiserum. Of the isolated S. mutans, one strain (P2-1) showed strong agglutinating reaction with antiserum, another strain (P1-2) showed weak agglutinating reaction. P2-1 strongly adhered to the wall of the test tube containing 5% sucrose broth, while p1-2 weakly colonized on the wall of the test tube. Biotyping of the isolated S. mutans based on the fermentation of mannitol, sorbitol, raffinose and melibiose, and the production of ammonia from L-arginine, and the inhibition of acid production by bacitracin. Biochemical characteristics of P2-1 strain correlated with the recognized biotype c, pl-2 strain resembled biotype d of S. mutans.

• 대한가정학회지
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• 제24권1호
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• pp.43-51
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• 1986

In order to study antioxidative acton of garlic, alliin, scordinin, garlic oil, ethanol fraction and non-kaolin fraction which have been discovered from garlic until now were extracted and isolated, and each fraction was utilized as the experimental materials. Antioxidative action of each fraction was compared through the in vitro and in vivo experiments. Electron donatingability on $\alpha$, $\alpha$-diphenyl-$\beta$picrylhydra-$\chi$yl, the inhibitory effect of lipoperoxide formation by TBA and peroxide value were measured and analyzed. RESULTS : 1. When observed antioxidative ability by EDA value, ethanol fraction of garlic components showed the strongest reaction as 15.25. 2. In vitro experiment with TBA value, garlic oil, alliin and ethanol fracton showed distinctive effect on inhibitory effect of lipoperoxide formation. 3. Comparing with the inhibitory effect of lipoperoxide formation with TBA value in vivo, the ethanol fraction was the most effective in the blood or liver by intraperitoneal administration, whereas the ethanol fraction in the blood and non-kaolin fraction in the liver was most effective each other by orally administration. 4. In vitro experiment with peroxide value, garlic oil was distinctive effect on the inhivitory effect of lipoperoxide formation, which was a similar to the trend of TBA value in vitro. 5. Examining the induction time for the first period of lipoperoxide formation in vitro, garlix oil, ethanol fraction and alliin were effective, which was a similar to the trend of TBA value and peroxide value in vitro.