• Korean Journal of Environmental Biology
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• v.20 no.4
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• pp.355-359
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• 2002

This study was carried out to investigate the combined effect of cold storage with irradiation on rooting of chrysanthemum cuttings. Each cutting was six cm in length with three real leaves. A set of Groups of the cuttings was irradiated with 0 to 70Gy of gamma-ray after four weeks of cold storage, while another groups were irradiated with the same doses and then stored at $4^\circ{C}$ for four weeks. Root and shoot growth were observed at 15 and 20 days after planting of the cuttings in a Plug tray. Accelerated root formation was observed in 30 Gy irradiated group, and root growth was also enhanced in 20 and 30 Gy irradiated groups 20 days after planting. In case of irradiation combined with pre-treatment of cold storage, survival rate of cuttings was the highest (80%) in 30 Gy irradiated group. The groups treated with irradiation first before cold storage were apparently better in root formation, among which 50 Gy irradiated group showed the highest rate of root growth.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.21 no.2
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• pp.165-179
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• 1991

This study was performed to understand the irradiation effects on the mandibular condyle and mandibular growth in developing white rats. Forty eight white male rats of the Sprague-Dawley strain aged 4 weeks, were devided into two groups; control group and experimental group. A single target dose of l0Gy of radiation was given to the mandibular condylar area and the observations of the photo analysis, radiologic, histopathologic and immunohistochemical study revealed as follows; 1. Animals killed one week after irradiation showed lesser increase in body weights, no difference in photo analysis and decreased thickness of cartilagenous layers of the condyle than the control group. 2. Two weeks after irradiation the weight increases were almost same in both irradiated and control groups and in photo analysis, the distance from Mental Foramen to Incisal tip (Mf-It) was longer than the controls. Repair processes were taken place in irradiated group, but the cartilagenous layers were thinner than the controls. 3. By the third week after exposure further repair was seen in the trabeculae and the distance from Condylion to Mandibular plane (Cd-Cd') was longer than the controls and the weight increases were almost same as the controls. 4. At 4 weeks after irradiation the cells of proliferating zone repaired to almost normal findings, but the cartilagenous cell layers were still thinner than the control animals. In photo analysis, the distances from Menton to Anterior Notch (Me-An), from First Molar to Mandibular plane (Fm-Fm') were shorter and the weight increases were lesser than the controls. 5. In the S-100 antibody, the positive cells were increased in number, but decreased reactivities were seen at the proliferating zone of the irradiated groups. In the Monoclonal Anti-Proteoglycan antibody and Type Ⅰ collagen antibody, the irradiated groups showed little decreased number of positive cells and in the Type Ⅱ collagen antibody, the differences between irradiated and control groups were undetectable in immunohistochemical study.

• Food Science and Preservation
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• v.16 no.3
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• pp.454-458
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• 2009

The effect of UV-C irradiation on microbial growth and quality of red pepper powder during storage was examined. Red pepper powder was irradiated with doses of 27, 54, or $108\;kJ/m^2$ and stored at $20^{\circ}C$ for 4 weeks. UV-C treatment of red pepper powder decreased the populations of total aerobic bacteria and Bacillus cereus in proportion to radiation dose. In particular, total aerobic bacteria and B. cereus populations decreased by 1.03 and 0.90 log CFU/g after irradiation at $108\;kJ/m^2$, respectively, compared with control values. UV-C irradiation caused negligible changes in the Hunter color L, a, or b values. Sensory quality results on red pepper powder were not significantly different between treatments. Therefore, UV-C irradiation can be used to inhibit microbial growth in red pepper powder, without impairing quality during storage.

• Korean Journal of Organic Agriculture
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• v.21 no.4
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• pp.659-668
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• 2013

The objective of this study was carried out to elucidate the effect of LEDs (light emitting diodes) irradiation in relation to early growth and inorganic elements in leaf lettuce (Lactuca sativa L. 'Chung Chi Ma'). In morphological changes of leaves, shoot elongation and hypocotyl length showed poor growth in red light irradiation, while the red+blue light irradiation induced shorter plant height and much greater leaf numbers resulting in increased fresh weight. In change of the Hunter's color and SPAD values, lettuce seedlings grown under in red+blue and fluorescent light irradiation had a higher $a^*$ value, otherwise SPAD values were not changed in these light irradiations. Interestingly, relative chlorophyll contents showed 1.8 times increased redness in the treatment of red+blue light irradiation. Inorganic element (N, Ca, Mg, Mn, and Fe) and ascorbic acid contents were increased in lettuce plants grown under LEDs light irradiation compared to those of lettuce grown under the fluorescent light which showed higher P and Mn contents. In conclusion, it is considered that red+blue light irradiation which stimulates growth and higher nutrient uptake in leaf lettuce could be employed in containers equipped with LEDs.

• Korean Journal of Environmental Biology
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• v.20 no.3
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• pp.197-204
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• 2002

Gourd seeds were irradiated with the doses of 0-20 Gy to investigate the effect of the low dose $\gamma$-radiation on the early growth and physiological activity. The stimulating effects of the low dose y- radiation on the early growth were not noticeably high, but were increased generally at 4-16 Gy irradiation group. The catalase and peroxidase activity of cotyledon from seeds irradiated with $\gamma$- radiation were increased at 8 Gy irradiation group. The photochemical activity of leaf was noticeably high at 4 Gy irradiation group. The photochemical yield of PSII, estimated as Fv/Fm, decreased with increasing illumination time by 50% after 4 hrs in the control and 8 Gy irradiation group, while Fo slightly increased. However, Fv/Em in the 4 Gy irradiation group decreased by 40% of inhibition, indicating that photoinhibition decreased by the low dose $\gamma$- radiation. Changes in the effective quantum yield of PSII, $\varphi_{PSII}$ and 1/Fo- l/Fm, a measure of the rate constant of excitation trapping by the PSII reaction center, showed similar pattern to Fv/Em. NPQ decreased by 70% after photoinhibitory treatment with showing similar pattern between the control and the irradiation group. These results showed the positive effect of low dose $\gamma$- radiation on the seedling growth and the reduction of photoinhibition in the 4 Gy irradiation group.

• Korean Journal of Veterinary Research
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• v.13 no.1
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• pp.85-102
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• 1973

In the event a population of animals is exposed to ionizing radiation, proper disposition of animals will minimize loss to the farmer and protect the public from unwholesome meat and dairy products. Clinical response is an important factor in considering salvage of animals for food following exposure to ionizing radiation whether the dose is lethal or not. It is the purpose of this report to present the discussions of blood picture and growth of y ung growing rabbits exposed to cobalt-60 gamma-irradiation. The experimental animals were Hyrnalayan rabbits of 65 days old. The body weight for all animals of the time of irradiation was approximately 450g. The 96 rabbits used in the experiment were allotted t groups of eight. Rabbits in one group served as controls and the others were exposed to single doses of 100, 200, 300, 400, 500, 600 and 700 Roentgens (R). The exposure does rate averaged 15 R per minute. Central blood was obtained by heart puncture. Erythrocyte and leukocyte enumerations and, determinations of hemoglobin in blood and hematocrit value were made in the usual manner. Erythrocyte counts, concentrations of hemoglobin in blood, and hematocrit readings dropped to the lowest value between 1 and 4 weeks postirradiation, returning towards the control values. But recovery was not complete at the time of the last determination on 6 weeks postirradiation. There was a progressive decrease with increasing radiation does in the above values. Mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration were not affected by the irradiation. After irradiation of each does the numbers of total leukocyte and the absolute numbers of various types of white cell began to decrease rapidly, and reached a minimum after 2 weeks, returning towards normal levels. But recovery was not complete at the time of the last determination on 6 weeks postirradiation. The reductions in total leukocyte and differential counts as the radiation does increases were apparent. Relative neutrophilia was observed in each irradiated group because of more expense of lymphocytes after irradiation. Growth rate of the rabbits was not affected by 100 R of whloe-body irradiation, while rabbits irradiated with 200, 300, 400 and 500 R showed marked depression of growth rate. As the radiation does increases, a depression of growth rate was apparent. The rabbits exposed to 600 and 700 R ceased from growing and recovery was not recognized during the experimental period.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.1
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• pp.52-56
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• 1985

For the fundamental studies of radiation breeding in edible marine algae, the biological effects on conchospores of Porphyra species by gamma-irradiation were examined. Two varieties, Keun-cham-gim (Porphyra tenera Kjell. form tamatsuensis Miura) and Saga No.5, were chosen for this study, and their conchospores after r-irradiation($5.0{\sim}20.0$ KR) were cultured for 50 days. The results obtained were summarized as follows. 1. Gamma-irradiation in less than the dose of 20KR did not affect germination of conchospores, and almost all spores grew into two cells germ in 24 hours after irradiation, but withering germs were gradually increased in number according to higher exposure within 5 days old culture. 2. High irradiation caused the induction of giant cells, abnormal useless growth of hold-fast, lumpish thalli and callus-like lumpy tissues. 3. The liberation of neutral spores from young germs and carpospores from mature thalli were observed on the frond exposed at $10{\sim}20$ KR irradiation. All spores were normal in division and its size. 4. The best irradiation effect on growth of Keun-cham-gim was observed at 10 KR dose, whose growth-rates were $140\%$ in wet weight and $108\%$ in mean frond area, but only $48\%$ was recorded in wet weight at 20 KR exposure. Saga No.5 were in contrast with Keun-cham-gim, and their most growth-rate was $400\%$ in wet weight ($258\%$ in frond area) at 10 KR irradiation and the worst was $20\%$ at the dose of 20 KR. 5. The withering phenomenon to death by treatment of gamma-ray presented substantial difference between two varieties. Survival rate compared with control in Keun-cham-gim was $70.7\%$ at 20 KR, but that in Saga No.5 recorded $47.0\%$ at same dose. 6. Synthesizing the results of high and low r-irradiation, it was suggested tat high r-irradiation in more than 5.0 KR inhibited conspicuously the growth of germs derived from conchospores, and about half of them withered at 15.0 KR dose or more.

• Journal of Radiation Protection and Research
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• v.24 no.3
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• pp.123-129
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• 1999

Tomato (Lycopericum esculentum $M_{ILL}$ cv. Seokwang and cv. Housemomotaro) seeds were irradiated with the doses of $1{\sim}20$ Gy from $^{60}Co$ $\gamma$-ray source to investigate the effect of the low dose $\gamma$-ray radiation on the early growth and resistance to subsequent high dose of radiation. Germination rate of seeds irradiated with low dose $\gamma$-ray was enhanced in Seokwang cultivar but not in Housemomotaro cultivar. Seedling height increased in 4 Gy and 8 Gy irradiation group of both cultivars. Plant height of Seokwang cultivar was depressed in low dose irradiation group but fresh weight was increased in 2 Gy and 4 Gy irradiation group. In Housemomotaro cultivar, plant height increased in 12 Gy and 20 Gy irradiation group and fresh weight increased in 4 Gy and 20 Gy irradiation group. Growth inhibition of tomato plants by high dose radiation was noticeably reduced by pre-irradiation of low dose radiation. Resistance to subsequent high dose of radiation was enhanced in 2 Gy and 8 Gy Irradiation group of Seokwang cultivar and in 2 Gy and 12 Gy irradiation group of Housemomotaro cultivar.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.2
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• pp.193-199
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• 2015

We observed that the growth and physiological change in Haliotis discus discus by low-dose irradiation with gamma ray.Irradiation with gamma ray was undertaken by using the low-level irradiation facility ($^{60}CO$) in cooperation with the Institute of Nuclear Science and Technology at Jeju National University. The parent abalones were attached one by one and then fixed by using the rubber band to the front side of the fabricated case for irradiation with gamma ray. The experimental plots of irradiation with gamma ray were set as 10, 15, 20 and 25 Gy and the 25 female abalones and 10 male abalones were utilized for each experimental plot. The sperms and eggs were fertilized by setting an interval for each dose to prevent mixing with other experimental plots when fertilizing the sperms and eggs for each dose of irradiation with gamma ray. As for the fertility, it was confirmed to be 85% the control and 10 Gy groups, whereas it was found to be 80%, 65% and 50% in the 15 Gy, 20 Gy and 25 Gy groups, respectively. As a conclusion, the hatching rate and attachment rate were higher at 10 and 15 Gy than the other experimental plots, and the growth rate was higher at 20 Gy than the other experimental plots. Also the changes in lysozyme activity in accordance with the stress of water temperature were found to have a significant increase in the other experimental plots as compared with the control plot at the end of 0 h. The changes in lysozyme activity have remained constant in all the experimental plots at the end of 12 h. These results allowed us to confirm that lysozyme was undertaking the biodefense action by reacting sensitively to the stress of water temperature in the control experimental plot. As for the other experimental plots, they are believed to avoid the biodefense mechanism due to the high degree of anti-parasite mechanism and anti-viral mechanism. Thus, it is believed that it would be imperative to conduct development and research on breeds that were potent for environmental tolerance by applying the method of irradiation with gamma ray to other marine animals and plants.

• Korean Journal of Environmental Agriculture
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• v.24 no.2
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• pp.153-158
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• 2005

To investigate alleviation effects of salt stress by low dose gamma-irradiation on seedling growth of rice (Oryza shrive L), in plants of two rice cultivars (Ilpumbyeo and Gancheokbyeo), seeds were irradiated with the different doses $(0{\sim}32\;Gy)$ of gamma-ray by irradiator ($^{60}Co$, ca.150 TBq of capacity, AECL). The salt stress caused a remarkable decrease of seedling growth, particularly for dry weight. In addition, relative growth rate was decreased over 50% by the salt stress. However, the salt stress-induced inhibition of growth was alleviated by gamma-irradiation. Especially, the relative growth rate of shoot showed the reduction effect over 30% at 4 Gy-irradiated groups as compared with that of control. Also, electrolyte leakage and relative water content were damaged, respectively 4 times and 30% by the salt stress but the damages were alleviated by the gamma-irradiation. These alleviation effects were observed similarly in both the cultivars tested. These results suggest that the low dose gamma-irradiation may have a better protection or the salt stress.