• 한국산학기술학회논문지
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• 제12권9호
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• pp.4068-4074
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• 2011

본 연구는 유방암 환자를 대상으로 MR Breast perfusion 영상과 시간-신호강도 곡선의 진단적 유용성을 평가하고자 하였다. 2009년 3월부터 2010년 12월까지 조직학적으로 관상피 내암으로 진단 받은 환자 20명을 대상으로 하였다. 우선 역동적 조영 증강 영상을 획득한 후 Breast perfusion 영상을 재구성 하였다. 재구성 된 영상은 시간 강도 그래프를 획득 한 후 병변 부위, 정상 부위, 백그라운드 부위 등 세부위에서 기울기, 최대 상대 조영증강, 최대 조영 증강 시간을 측정 하였다. 실험에 대한 정량적 분석방법으로 병변 부위와 정상 부위, 백그라운드 부위의 기울기를 측정하여 평균값을 비교 분석 하였다. 정성적 분석에서는 육안적으로 각 픽셀의 신호 강도를 분석 하였고 높음(3점), 중간(2점), 낮음(1점)의 구분을 3점 척도로 실시하여 평균값을 측정하였다. 정량적 분석 결과 기울기와 최대 상대 조영증강은 병변 부위에서 가장 높았으며 최대 조영 증강 시간은 백그라운드 부위에서 가장 높았다. 정성적 분석에서는 Breast perfusion 영상은 진단적 가치가 높았다.

• Journal of Breast Cancer
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• 제21권4호
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• pp.406-414
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• 2018

Purpose: T-cell immunoglobulin and mucin domain-containing molecule 3 (TIM-3) is an emerging immune response molecule related to T-cell anergy. There has been tremendous interest in breast cancer targeting immune checkpoint molecules, especially in the triple-negative breast cancer (TNBC). This study was designed to investigate TIM-3 expression on tumor infiltrating lymphocytes (TILs), its relationships with clinicopathological parameters and expression of programmed death receptor 1 (PD-1)/programmed death receptor ligand 1 (PD-L1), and its prognostic role. Methods: Immunohistochemistry on tissue microarray blocks produced from 109 samples of invasive ductal carcinoma type TNBC was performed with antibodies toward TIM-3, PD-1, PD-L1 and breast cancer-related molecular markers. Associations between their expression and clinicopathological parameters as well as survival analyses were performed. Results: TIM-3 was expressed in TILs from all 109 TNBCs, consisting of 17 cases (<5%), 31 cases (6%-25%), 48 cases (26%-50%), and 13 cases (>51%). High TIM-3 was significantly correlated with younger patients (p=0.0101), high TILs (p=0.0029), high tumor stage (p=0.0018), high PD-1 (p=0.0001) and high PD-L1 (p=0.0019), and tended to be associated with higher histologic grade, absence of extensive in situ components and microcalcification. High TIM-3 expression was significantly associated with a combinational immunophenotype group of high PD-L1 and high PD-1 (p<0.0001). High TIM-3 demonstrated a significantly better disease-free survival (DFS) (p<0.0001) and longer overall survival (OS) (p=0.0001), together with high TILs and high PD-1. In univariate survival analysis, high TIM-3 showed reduced relapse risk (p<0.0001) and longer OS (p=0.0003), together with high PD-1 expression. In multivariate analysis, high TIM-3 was statistically significant in predicting prognosis, showing better DFS (hazard ratio [HR], 0.0994; 95% confidence interval [CI], 0.0296-0.3337; p=0.0002) and longer OS (HR, 0.1109; 95% CI, 0.0314-0.3912; p=0.0006). Conclusion: In this study, we demonstrate that TIM-3 expression is an independent positive prognostic factor in TNBC, despite its association with poor clinical and pathologic features.