• Journal of Society of Preventive Korean Medicine
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• v.7 no.1
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• pp.47-53
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• 2003

New method to obtain Intravascular Bonghan ducts of rats and mice were developed By extracting blood from caudal veins and arteries that were hold by forceps we took broken pieces of BHDs which were examined using microscopes. The advantage of this method is to gain the pure BHD without fibrin, and its disadvantage is the smallness of the sample.

• Journal of Veterinary Clinics
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• v.27 no.4
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• pp.415-420
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• 2010

During a last decade, there have been considerable reports of novel thread-like structures (NTSs) in animals. The NTSs are thought to be a part of the Bonghan Ducts (BHD) system, which is described as a newly-discovered circulatory system. Networks of BHD system are completely different from blood vessels or lymphatic system in animal. To demonstrate intravascular NTSs, it is necessary to develop the effective method to observe the NTSs morphology. We considered several methods to study morphologically with NTSs. There are not explaining such structure in the current knowledge of anatomy and physiology. But we found very effective and easy method to study NTSs and BHD by agar-mediated embedding method.