• Title/Summary/Keyword: Intra-molecular

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Genetic Variation of Rice Populations Estimated Using nrDNA ITS Region Sequence

  • Wang, Dong;Hong, Soon-Kwan
    • Korean Journal of Plant Resources
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    • v.27 no.3
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    • pp.249-255
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    • 2014
  • The rice belonging to Oryza sativa is not only has significant economic importance, for it is the major source of nutrition for about 3 billion all around the world. But also plays a vital role as a model organism, because it has a number of advantages to be a model plant, such as efficient transformation system and small genome size. Many methods and techniques have been conducted to attempt to distinguish different Oryza sativa species, such as amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR) and so on. However, studies using sequence analysis of internal transcribed spacer (ITS), a region of ribosomal RNA has not been reported until now. This study was undertaken with an aim to understand the phylogenetic relationships among sixteen isolates of Oryza sativa collected from abroad and fifteen isolates collected from Korea, using ribosomal RNA (rRNA) internal transcribed spacer (ITS) sequences to compare the phylogeny relationships among different Oryza sativa species. The size variation obtained among sequenced nuclear ribosomal DNA (nrDNA) ITS region ranged from 515bp to 1000bp. The highest interspecific genetic distance (GD) was found between Sfejare 45 (FR12) and Anapuruna (FR15). Taebong isolate showed the least dissimilarity of the ITS region sequence with other thirty isolates. This consequence will help us further understanding molecular diversification in intra-species population and their phylogenetic analysis.

Intraspecific Molecular Phylogeny, Genetic Variation and Phylogeography of Reticulitermes speratus (Isoptera:Rhinotermitidae)

  • Park, Yung Chul;Kitade, Osamu;Schwarz, Michael;Kim, Joo Pil;Kim, Won
    • Molecules and Cells
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    • v.21 no.1
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    • pp.89-103
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    • 2006
  • Population structure was investigated in Reticulitermes speratus populations in the Korean Peninsula and the Japanese Archipelago. All trees derived from analyses of the combined sequence dataset of two mitochondrial genes, COII and COIII, showed that R. speratus populations cluster into two major clades comprising the Korean/southern Japanese populations and the northern Japanese populations. Analysis of population genetic structure showed strong genetic partitioning between populations of the two clades. To understand historical migration routes and current distributions, the phylogeographic history of R. speratus was inferred from intra-/interspecific phylogeny and divergence times estimated between the clades of the phylogenetic tree. The estimated migration route and divergence time of ancestral R. speratus are congruent with recent paleogeographic hypotheses involving land-bridge connections between the Asian continent and the Japanese Archipelago. We suggest that ancestral R. speratus separated into northern and southern Japanese populations after its migration into the Japanese main islands from East China during the early Pleistocene via the East China Sea basin, which may have been exposed during that period. The Korean populations seem to have diverged recently from southern Japanese populations; this may explain the current distribution of R. speratus in the Japanese Arachipelago, and account for why it is restricted to northern areas of the Tokara Strait.

Inter- and Intra-granular Critical Current in $Bi_{1.4}Pb_{0.6}Sr_2Ca_2Cu_{3.6}O_x$ Superconducting Oxide

  • Choy, Jin-Ho;Kim, Seung-Joo;Park, J.C.;Frohlich, K.;Dordor, P.;Grenier, J.C.
    • Bulletin of the Korean Chemical Society
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    • v.11 no.6
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    • pp.560-563
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    • 1990
  • A.c. susceptibility for $Bi_{1.4}Pb_{0.6}Sr_2Ca_2Cu_{3.6}O_x$ superconductor is measured as a function of temperature at different value of a.c. magnetic field amplitude. Two transition steps are attributed to the intergranular and intragranular properties. Based on Bean's critical state model, intergranular critical current density, $J_c^{gb}$ (11 $A/cm^2$ at 77 K) and intragranular critical current density, $J_c^g (7{\times}10^3\;A/cm^2$ at 100 K) are estimated. The low values of $J_c^{gb}$and $J_c^g$ reflect a poor nature of coupling between grains and the low pinning force density of intragrain in $Bi_{1.4}Pb_{0.6}Sr_2Ca_2Cu_{3.6}O_x$ superconductor.

Trichinella Infection Ameliorated Vincristine-Induced Neuroinflammation in Mice

  • Jo, Young Rae;Park, Hwan Tae;Yu, Hak Sun;Kong, Hyun-Hee
    • Parasites, Hosts and Diseases
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    • v.60 no.4
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    • pp.247-254
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    • 2022
  • Vincristine (VCR) is a chemotherapeutic agent widely used in treatment of malignancies. However, VCR has a limitation in use since it commonly causes a painful neuropathy (VCR-induced peripheral neuropathy, VIPN). Inflammatory cytokines secreted by immune cells such as macrophages can exacerbate allodynia and hyperalgesia, because inhibiting the inflammatory response is a treatment target for VIPN. In this study, we investigated whether Trichinella spiralis, a widely studied helminth for its immunomodulatory abilities, can alleviate VCR-induced allodynia. Von Frey test showed that T. spiralis infection improved mechanical allodynia at 10 days after VCR injection. We further observed whether the difference was due to mitigated axon degeneration, but no significant difference between the groups in axonal degeneration in sciatic nerves and intra-epidermal nerve fibers was found. Conversely, we observed that number of infiltrated macrophages was decreased in the sciatic nerves of the T. spiralis infected mice. Moreover, treatment of T. spiralis excretory-secretory products caused peritoneal macrophages to secrete decreased level of IL-1β. This study suggests that T. spiralis can relieve VCR-induced mechanical allodynia by suppressing neuroinflammation and that application of controllable degree of helminth may prove beneficial for VIPN treatment.

Controll over the Au@Ag Core-shell Nanoparticle 2D Patterns via Diblock Copolymer Inverse Micelle Templates and Investigation of the Surface Plasmon Based Optical Property (이중블록공중합체 역마이셀 주형을 이용한 Au@Ag 코어-쉘 나노입자 2차원 패턴 제어 및 표면 플라즈몬 기반 광학적 특성 연구)

  • Yoon, Min Ji;Kim, Jihyeon;Jang, Yoon Hee;Lee, Ji-Eun;Chung, Kyungwha;Quan, Li Na;Kim, Dong Ha
    • Journal of the Korean Chemical Society
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    • v.57 no.5
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    • pp.618-624
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    • 2013
  • We demonstrated unique inter- and intra-plasmonic coupling effects in bimetallic Au@Ag core-shell NP arrays which are regularly or randomly arranged on self-assembled block copolymer (BCP) inverse micelle monolayers. Polyvinylpyrrolidone (PVP)-stabilized Au@Ag core-shell NP arrays in regular or disordered configuration were incorporated and assembled on reconstructed PS-b-P4VP inverse micelle templates through two types of processes. The intensively enhanced LSPR coupling properties of individual and assembled Au@Ag NPs were evaluated by UV-visible spectroscopy in terms of the type of ligand stabilizer, coupling between Au and Ag, thickness of Ag shell, and type of array configuration. Finally, Au@Ag core-shell NP arrays were employed as active substrates for surface enhanced Raman spectroscopy (SERS) and a significantly enhanced signal enhancement was observed in accordance with the coupling intensity of Au@Ag NPs patterns.

Neuroglial Cells and Schizophrenia (신경아교세포와 조현병)

  • Won, Seunghee
    • Korean Journal of Biological Psychiatry
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    • v.22 no.2
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    • pp.47-54
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    • 2015
  • In the past decade, structural, molecular, and functional changes in glial cells have become a major focus in the search for the neurobiological foundations of schizophrenia. Glial cells, consisting of oligodendrocytes, astrocytes, microglia, and nerve/glial antigen 2-positive cells, constitute a major cell population in the central nervous system. There is accumulating evidence of reduced numbers of oligodendrocytes and altered expression of myelin/oligodendrocyte-related genes that might explain the white matter abnormalities and altered inter- and intra-hemispheric connectivities that are characteristic signs of schizophrenia. Astrocytes play a key role in the synaptic metabolism of neurotransmitters ; thus, astrocyte dysfunction may contribute to certain aspects of altered neurotransmission in schizophrenia. Increased densities of microglial cells and aberrant expression of microglia-related surface markers in schizophrenia suggest that immunological/inflammatory factors are of considerable relevance to the pathophysiology of psychosis. This review describes current evidence for the multifaceted role of glial cells in schizophrenia and discusses efforts to develop glia-directed therapies for the treatment of the disease.

Dictyota bartayresiana, a Brown Algal Species Newly Recorded in Korea

  • Kang, Pil Joon;Nam, Ki Wan
    • Korean Journal of Environmental Biology
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    • v.35 no.3
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    • pp.335-340
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    • 2017
  • A marine brown alga was collected from Geoje, located on the southern coast of Korea. This species shares the generic features of Dictyota and is morphologically characterized by the combined features of erect or prostrate thallus with smooth margin, dichotomous to subdichotomous branches not twisted at the terminal part, absence of surface proliferations, 1-3 cell layered cortex and unilayered medulla, and lack of involucrum in sporangia subtended by a single stalk cell. In a phylogenetic tree based on rbcL sequences, this species nests in the same clade with Dictyota bartayresiana. The genetic distance between both sequences within the clade was calculated as 1.2%, considered to be within the intra-species range for the genus. Based on these morphological and molecular analyses, this Korean alga is identified as D. bartayresiana described originally from the West Indies. This is the first record of D. bartayresiana in Korea.

SECONDARY ELECTRONS IN CLUSTERS OF GALAXIES AND GALAXIES

  • HWANG CHORNG- YUAN
    • Journal of The Korean Astronomical Society
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    • v.37 no.5
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    • pp.461-463
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    • 2004
  • We investigate the role of secondary electrons in galaxy clusters and in ultra-luminous infrared galaxies (ULIGs). The radio emission in galaxy clusters and ULIGs is believed to be produced by the synchrotron radiation of relativistic electrons. Nonetheless, the sources of these relativistic electrons are still unclear. Relativistic secondary electrons can be produced from the hadronic interactions of cosmic-ray nuclei with the intra-cluster media (ICM) of galaxy clusters and the dense molecular clouds of ULIGs. We estimate the contribution of the secondary electrons in galaxy clusters and ULIGs by comparing observational results with theoretical calculations for the radio emission in these sources. We find that the radio halos of galaxy clusters can not be produced from the secondary electrons; on the other hand, at least for some ULIGs, the radio emission can be dominated by the synchrotron emission of the secondary electrons.

Time Dependence of the Rheological Properties of the Solutions of Polyacrylontrile and Acrylonitrile-Itaconic Acid Copolymer in Dimethyl sulfoxide (아크릴 중합체 및 아크릴-이타코닉산 공중합체/ 디메틸술폭시드 용액의 유변학적 특성의 시간의존성)

  • 이남순
    • Textile Coloration and Finishing
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    • v.13 no.6
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    • pp.435-440
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    • 2001
  • This study investigates the rheological properties of in-situ polymerized solutions of polyacrylonitrile(PAN) and acrylonitrile(AN) -itaconic acid(IA) in dimethyl sulfoxide(DMSO) in terms of temperature, concentration, and time. The complex viscosity and storage modulus of the solutions were generally increased with elapsing time, which is ascribable to the three-dimensional pseudostructures formed by strong inter- or Intra-molecular attractions through Polar -CN and -COOH groups. The three-dimensional pseudonetworks would lead to relation of the acrylic solutions in long term. This was more noticeable at higher temperature within the temperature range examined. In the case of 20% solutions one can not observe lower Newtonian flow region in the viscosity curve. Disappearance of lower Newtonian flow region is indicative of heterogeneity of the solution system. Casson Plot of the viscosity data revealed that 20% solutions of PAN and AN-IA copolymer in DMSO clearly demonstrated positive yield stress, ascertaining formation of pseudostructures in the solution systems.

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Time Dependence of the Rheological Properties of the Solutions of Polyacrylontrile and Acrylonitrile-Itaconic Acid Copolymer in Dimethyl sulfoxide (아크릴 중합체 및 아크릴-이타코닉산 공중합체/디메틸술폭시드 용액의 유변학적 특성의 시간의존성)

  • Lee, Nam Sun
    • Textile Coloration and Finishing
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    • v.13 no.6
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    • pp.77-77
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    • 2001
  • This study investigates the rheological properties of in-situ polymerized solutions of polyacrylonitrile(PAN) and acrylonitrile(AN)-itaconic acid(IA) in dimethyl sulfoxide(DMSO) in terms of temperature, concentration, and time. The complex viscosity and storage modulus of the solutions were generally increased with elapsing time, which is ascribable to the three-dimensional pseudostructures formed by strong inter- or intra-molecular attractions through Polar -CN and -COOH groups. The three-dimensional pseudonetworks would lead to gelation of the acrylic solutions in long term. This was more noticeable at higher temperature within the temperature range examined. In the case of 20% solutions one can not observe lower Newtonian flow region in the viscosity curve. Disappearance of lower Newtonian flow region is indicative of heterogeneity of the solution system. Casson Plot of the viscosity data revealed that 20% solutions of PAN and AN-IA copolymer in DMSO clearly demonstrated positive yield stress, ascertaining formation of pseudostructures in the solution systems.