• BMB Reports
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• v.28 no.5
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• pp.437-442
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• 1995

The potency of yeast-derived methionyl-free human growth hormone (rhGH), which was obtained by removal of the N-terminal Met from methionyl-hGH, was estimated by in vitro and in vivo assays. In radio-receptor assay where the binding affinity of growth hormone to the receptor was estimated, the recombinant hGH showed 2.9 international units (IU) per mg of specific activity. In contrast, pitUitary-derived human growth hormone had a slightly lower receptor binding activity (2.5 IU/mg) compared with recombinant growth hormone. For the in vivo assay, efficacy of rhGH was tested by use of hypophysectomized rats, in which pituitary organs were surgically removed, resulting in the termination of growth hormone secretion. The weight-increase in rats by the injection of rhGH was almost identical to the result obtained by the injection of the same amount of pituitary-derived (international standard) hGH. A comparision of the secondary structures of rhGH and rMet-hGH by circular dichroism spectrophotometer demonstrated that the removal of the methionyl residue from rMet-hGH did not exert any effect on the structure of the growth hormone. In conclusion, methionyl-free human growth hormone produced from yeast was highly potent in biological activity and maintained a legitimate three dimensional structure.

• Analytical Science and Technology
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• v.9 no.2
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• pp.145-160
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• 1996

Inorganic analytical laboratory of Korea Research Institute of Standards and Science participated in an interlaboratory comparison program operated by Quebec Toxicology Centre of Canada in 1994 and again in 1995. The objective of this program is to enable participating laboratories to assess reproducibility and accuracy of their analytical results for trace toxic elements in human biological fluids. This laboratory determined Cd and Pb concentrations in 3 levels of human blood samples by isotope dilution inductively coupled plasma mass spectrometry. 0.5mL of blood sample is added to the digestion bomb together with 2mL of nitric acid and enriched spike isotopes and then decomposed in the microwave digestion system. The decomposed sample is diluted to 10mL and nebulized into ICP-MS. The Cd and Pb values reported by all participating laboratories are presented and compared. The values reported by this laboratory are within the acceptable range of target values.

• Korean Journal of Medicinal Crop Science
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• v.2 no.2
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• pp.174-179
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• 1994

This experiment was carried out to get the basic information on utilizing artemisia selengensis, the chemical component of artemisia selengensis and those Artemisia sp. were analyzed and compared. The results are as followed; The contents of water, crude ash and crude fiber in Artemisia selengensis were higer than in Artemisia sp. the contents of thiamin, riboflavin and ascorbic acid in Artemisia selengensis were a little lower than those in Artemisia sp. while niacin in Artemisia selengensis was a little higher than those in Artemisia sp. The content of dietary fiber were 40.91% for Artemisia selengensis and 4166.7mg% for Artemisia sp. and the contents of Na, Fe and Mn in Artemisia selengensis were over twice as a high as those in Artemisia sp. While the content of Cu was Artemisia selengensis was lower than that in Artemisia sp. The content of phenolic compound in Artemisia selengensis was a little higher than those in Artemisia sp.

• Journal of Advanced Marine Engineering and Technology
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• v.38 no.9
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• pp.1057-1063
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• 2014

Concerning $CO_2$ reduction from International Societies, lots of laboratories and relevant societies suggest many reports on how to reduce fuel consumption from their specific ways. Undoubtedly, cutting costs is the final desired destination for owner outcome, but many questions there are on the way yet: how is this measure working? how efficient is it? On what size of ship would it work best and be the most effective? etc. Fuel cost is one of the major cost elements for ship owners and/or operators. And by reducing fuel consumption owners and/or operators will reduce both their costs and the environmental impact from their ship. This paper is aim to address how the measures work for saving fuel consumption through improve propulsion efficiency, installation cost and benefit can be calculated easily in the return on investment for estimated one year operation, and finally their compatibility with other fuel saving measure devices.

• The Journal of the Acoustical Society of Korea
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• v.18 no.6
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• pp.77-83
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• 1999

H.263 is an international standard of ITU-T that can makes the service such as video phone, video conference in the transmission line less than 64Kbps. This recommendation draft has used motion estimation/compensation, transform coding and quantizing methods. TMN5 used for the performance estimation of H.263 has fundamentally used DCT in transform coding method and presented quantizer for quantizing the DCT transform coefficient. This paper is presenting adaptive quantizer effectively able to quantize DCT coefficient considering the human visual sensitivity while the structure of TMN5 is maintaining. As quantizer that proposed DCT-based H.263 could make transmit more frame than TMN5 in a same transfer speed, it could lower the frame drop effect. And the luminance signal appeared the difference of -0.3 ～ +0.7dB in the average PSNR for the estimation of objective image quality and the chrominance signal appeared the improvement in about 1.5dB in comparision with TMN5. As a result it can attain the better image quality compared to TMN5 in the estimation of subjective image quality.

• Journal of The Korean Association For Science Education
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• v.18 no.1
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• pp.19-34
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• 1998

This study compared junior high school science textbooks(for grade $7{\sim}9$) of the USA, Australia, Japan, and Korea. Specifically, the amount and pattern of knowledge, process skills, activities, contexts, nature of science, and integration topics were compared in terms of country, grade, and content. The results of analysis are as follow: 1) Physics, chemistry, biology, and earth science were equally distributed in textbooks of Korea and Japan. In Korean textbooks, "law" was more emphasized compared with other countries' textbooks. 2) The most popular process skills were proportion and controlling variables in Korea's textbooks. Correlation, cause/effect, and proportion in Japan's textbooks. Controlling variables and data transformation in textbooks of USA and Australia. 3) Experiment and observation were the most popular activities in textbooks of Korea, Japan, and Australia. In textbooks of the USA, observation, experiment, survey, practice, and measurement activities were used evenly. 4) In textbooks of Korea and Japan, pure science and natural environment contexts were the most popular. 5) There was no chapter dealing with nature of science in textbooks of Korea and Japan. 6) Integration topics between science and technology were the most popular. Based on these findings, several implications on Korean science textbooks were discussed.