• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.155-170
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• 2018

In this paper, mechanisms of gene editing technologies including ZFN, TALENS and CRISPR were briefly discussed with mutual advantages and disadvantages. Classification criteria of gene edited, site-directed mutagenesis (SDN) crops for regulatory purpose were also discussed. The number of studies using CRISPR technology was high and studies conducted on Arabidopsis thaliana and rice were highest, followed by tobacco, tomato, wheat, and corn. It has been applied to a variety of plants such as other grain crops, flower crops, vegetable crops, and fruit trees. The number of studies focused on practical application or commercialization in the future were also increasing yearly, and the scope of studies also expanded to include research on metabolic engineering for mass production of useful proteins or substances, development of disease resistant crops against viruses, bacteria, and fungi, abiotic environmental stressresistant crops, and increased yields. In addition to this, it was revealed that application range is becoming more diversified, including the development of parthenocarpic tomatoes, hybrid rice lines using male sterility and increased shattering resistance Brassica napus. It was also revealed that the number of CRISPR gene edited crops permitted by the USDA(APHIS) increases yearly, to be released in the international seed market soon.

• International Area Studies Review
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• v.15 no.3
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• pp.583-603
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• 2011

This study was designed to comprehensively understand and separate the characteristics of recreation forests of Korea through the multi-variate analysis for the provision of fundamental materials for recreation forests development policies and planning, and attempted to evaluate on the validity of classification of recreation forests, which has never been performed before. According to analysis results, recreation forests could be divided into 4 types; type I(recreation forests whose ecological nature status is not good, but the risk of calamity is low and accessibility is good), type II(recreation forests whose the risk of calamity is low, but ecological nature status is not good, slope is steep, and accessibility is poor), type III(recreation forests that have charming vegetation and landscape, various rest opportunity, and a gentle slope, but poor accessibility), and type IV(recreation forests that ecological nature status is good, but have poor landscape, less rest opportunity, and a steep slope). Also, each type was adjusted as follows; type I($25{\rightarrow}24$ reviews), type II($25{\rightarrow}26$ reviews), type III($17{\rightarrow}16$ reviews), and type IV($25{\rightarrow}25$ reviews). This study can be said to show the usability of validation of classified types, which was not done before. It is clear that the classification of recreation forests increases the efficiency in policy making and performance. Furthermore, by validating the separated types, we could expect high efficiency in policy making and performance on planned recreation forests in the future.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.4
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• pp.336-341
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• 1996

This study was conducted to investigate the effects of compost application on the soil loss and physico-chemical properties at the slope land from 1990 to 1991. Lysimeters with 15% slope. 5m slope length. 2m width and 1.2m depth were constructed in 1984 and filled with sandy loam, loam, clay loam. and clay soils. Treatments were bare soil, compost(1.5ton/10a) and non-compost with soybean-barley cropping system from 1984. Losses of soil were increased with the order of sandy loam, clay, loam and clay loam. Run-off was increased with the order of sandy loam, loam, clay loam and clay, but leachate was decreased with the same order. Compost treatments decreased 33.6-44.6% of soil loss and 17.0-24.0% of run-off but increased 17.1-33.7.% of leachate as compared with the non-compost treatments. The amount of soil loss was positively correlated with the amount of run-off by Y = 12.125+0.063X (r=$0.970^{**}$)and negatively correlated with the leachate by Y=43.425-0.096X(r=$-0.917^{**}$). The application of compost increased soil pH, OM, CEC and extractable cations. Application of compost decreased bulk density but increased porosity, water stable aggregate and available water. These results provide that the compost application plays an important role in conserving soil and water, and improving soil physico-chemical properties.

• Journal of Wetlands Research
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• v.16 no.2
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• pp.251-259
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• 2014

Recently, wetland and paddy wetland are being highlighted as the international environmental issues. However, research system which can assess paddy wetland is lacking. Thus, the purpose of this study is to assess the value of paddy wetland by applying RAM to paddy wetland. In addition, we would like to use this study as the data to establish assessment method for paddy wetland. 16 sites were selected as research targets based on altitude and soil. As the results of wetland assessment, 16 research target sites had total score of 212~227 and the average of 2.17~2.50. It was similar to those of Lacustrine Wetlands, Palustrine Wetland and Riverine Wetland which were sites in previous studies. The value could be recognized as the wetland. However, there was no difference in assessment results based on altitude and soil. It was found that all conservation values were the same. The factor the most closely affecting conservation value was the area. However, there was limitation to apply existing wetland assessment system to paddy wetland. In order to assess paddy wetland, factors such as rice farming methods, topography, vegetation, growth environment and biodiversity should be added. It was thought to supplement wetland assessment system through various further studies.

• Journal of agriculture & life science
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• v.52 no.6
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• pp.155-162
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• 2018

This study attempts to analyze the consumers' preference on Korean Red Ginseng Tonic, which is an essential product to revitalize Korean ginseng industry, using choice experiment method. The attributes used in the choice experiment were the ginseng age, good agricultural products(GAP), sugar, and price. A total of 1,796 experiments were collected and the value of each preferred attributes was estimated using a multinomial logistic model. The result shows that the products made from six-year-old and GAP(Good Agriculture Practice) approved Korean ginseng with less sugar were preferred. These estimated monetary values of marginal willingness to pay were about 94,000 KRW, 89,000 KRW, 5,000 KRW, respectively. Thus, the efforts to introduce and advertize GAP approved ginseng while developing new products with preferred attributes by general publics are necessary in the short run. In addition, we may need to consider developing the way to promote products using 4- and 5-year-old ginsengs, which are relatively underestimated in their health effectiveness but highly productive for farmers in the long run.

• Korean Journal of Environmental Agriculture
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• v.42 no.1
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• pp.71-81
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• 2023

An accurate and easy-to-use analytical method for determining isocycloseram and its metabolites (SYN549431 and SYN548569) residue is necessary in various food matrixes. Additionally, this method should satisfy domestic and international guidelines (Ministry of Food and Drug Safety and Codex Alimentarius Commission CAC/GL 40). Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) was used to determine the isocycloseram and its metabolites residue in foods. To determine the residue and its metabolites, a sample was extracted with 20 mL of 0.1% formic acid in acetonitrile, 4 g magnesium sulfate anhydrous and 1 g sodium chloride and centrifuged (4,700 G, 10 min, 4℃). To remove the interferences and moisture, d-SPE cartridge was performed before LC-MS/MS analysis with C₁₈ column. To verify the method, a total of five agricultural commodities (hulled rice, potato, soybean, mandarin, and red pepper) were used as a representative group. The matrix-matched calibration curves were confirmed with coefficients of determination (R²) ≥ 0.99 at a calibration range of 0.001-0.05 mg/kg. The limits of detection and quantification were 0.003 and 0.01 mg/kg, respectively. Mean average recoveries were 71.5-109.8% and precision was less than 10% for all five samples. In addition, inter-laboratory validation testing revealed that average recovery was 75.4-107.0% and the coefficient of variation (CV) was below 19.4%. The method is suitable for MFDS, CODEX, and EU guideline for residue analysis. Thus, this method can be useful for determining the residue in various food matrixes in routine analysis.

• Animal Bioscience
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• v.37 no.7
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• pp.1156-1167
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• 2024

Objective: MicroRNAs (miRNAs) are endogenous non-coding RNAs that can play a role in the post-transcriptional regulation of mammalian preadipocyte differentiation. However, the precise functional mechanism of its regulation of fat metabolism is not fully understood. Methods: We identified bta-miR-365-3p, which specifically targets the 3' untranslated region (3'UTR) of the FK506-binding protein 5 (FKBP5), and verified its mechanisms for regulating expression and involvement in adipogenesis. Results: In this study, we found that the overexpression of bta-miR-365-3p significantly decreased the lipid accumulation and triglyceride content in the adipocytes. Compared to inhibiting bta-miR-36 5-3p group, overexpression of bta-miR-365-3p can inhibit the expression of adipocyte differentiation-related genes C/EBPα and PPARγ. The dual-luciferase reporter system further validated the targeting relationship between bta-miR-365-3p and FKBP5. FKBP5 mRNA and protein expression were detected by quantitative real-time polymerase chain reaction and Western blot. Overexpression of bta-miR-365-3p significantly down-regulated FKBP5 expression, while inhibition of bta-miR-365-3p showed the opposite, indicating that bta-miR-365-3p negatively regulates FKBP5. Adenosine 5'-monophosphate (AMP)-activated protein kinase/mammalian target of rapamycin (AMPK/mTOR) signaling pathway is closely related to the regulation of cell growth and is involved in the development of bovine adipocytes. In this study, overexpression of bta-miR-365-3p significantly inhibited mRNA and protein expression of AMPK, mTOR, and SREBP1 genes, while the inhibition of bta-miR-365-3p expression was contrary to these results. Overexpression of FKBP5 significantly upregulated AMPK, mTOR, and SREBP1 gene expression, while inhibition of FKBP5 expression was contrary to the above experimental results. Conclusion: In conclusion, these results indicate that bta-miR-365-3p may be involved in the AMPK/mTOR signaling pathway in regulating Yanbian yellow cattle preadipocytes differentiation by targeting the FKBP5 gene.

• Journal of Mushroom
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• v.14 no.4
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• pp.136-141
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• 2016

The production scale of mushrooms in Korea is approximately 600 billion won, which is 1.6% of Korea's gross agricultural output. In Korea, ca. 190,000 tons of mushrooms are harvested annually. Although the numbers of mushroom farms and cultivators are constantly decreasing, total mushroom yields are increasing owing to large-scale cultivation facilities and automation. The recent expansion of the well-being trend has caused an increase in mushroom consumption in Korea: the annual per capita mushroom was 3.9 kg ('13), whichis a little higher than that in Europe. Thus, mushroom export, mainly Flammulina velutipes and Pleurotus ostreatus, has increased since the mid-2000s. Recently, however, it is slightly reduced. Nevertheless, Vietnam, Hong Kong, the United States, and the Netherlands continue to export mushrooms, and Korea has increased its export to Australia, Canada, Southeast Asia, etc. Canned Agaricus bisporus, the first export of the Korean mushroom industry, reached it speak sales in 1977-1978. When Korea initiated trade with China in 1980, the international prices of mushrooms fell sharply, leading to shrinkage of the domestic markets. Spurred by the high demand to develop substitute goods for A. bisporus, the oyster mushroom (P. ostreatus) gained attention since it seemed to suit the taste of Korean consumers. Although the log cultivation technique for oyster mushroom was developed in the early 1970s, it required a great deal of labor. Thus, we developed the shelf cultivation technique, which is easier to manage and allows for mass production. In this technique, the growing shelf is made mafrom fermented rice straw, whichis the only P. ostreatus medium in the world and isused only in South Korea. After then, the use of cotton wastes as an additional material of medium, the productivity. Currently, we are developing a standard cultivation technique and environmental control system that can stably produce mushrooms throughout the year. The increase of oyster mushroom production may boostthe domestic market and contribute to industrial development. In addition, oyster mushroom production technology played a role in forming the basis for the development of bottle cultivation, which made mass production. In particular, bottle cultivation using liquid spawn could allow for the export of F. velutipes and Pleurotus eryngii. In addition, the white varieties of F. velutipes were second developed in the world after Japan. We also developed the new A. bisporus cultivar 'Saeah', which is easy to grow in Korea. In hopes to advance the mushroom industry, we will continue to develop cultivars with international competitive power and to improve cultivation techniques.

• Journal of Korea Water Resources Association
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• v.53 no.spc1
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• pp.765-772
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• 2020

The amount of water resources identified by water balance analysis are usually used to formulate water resources plans. However, this does not consider the trade in goods between countries. It is possible to use virtual water to come up with a supply and demand plan by looking at the export and import of products. This is because it looks comprehensively at the direct water use (water resources within the region) and indirect water use (water resources of other regions from imported products). Yet South Korea does not actively use the concept of virtual water in the national water resources plan. There is difficulty with calculating and identifying the appropriate virtual water amount as many of the research papers present only few of the cases or omit the calculation process. This paper introduces detailed water footprint values for calculating the virtual water trade for South Korea. The international movement, water footprint, and virtual water trade of agricultural and livestock products are presented and compared to existing research. The water footprint and virtual water research in this paper can be utilized as baseline data for future researchers.

• Journal of Climate Change Research
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• v.1 no.2
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• pp.121-131
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• 2010

This study was carried out to evaluate spatial variations in rice production areas by simulating rice growth and yield with CERES-Rice growth model under GCM $2{\times}CO_2$ climate change scenarios. A modified window version(v4.0) of CERES-Rice was used to simulate the growth and development of three varieties, representing early, medium, and late maturity classes. Simulated growth and yield data of the three cultivars under the climate for 1971 to 2000 was set as a reference. Compared with the current normal(1971 to 2000), heading period from transplanting to heading date decreased by 7~8 days for the climate in $2^{\circ}C$ increase over normal, and 16~18 days for the climate in UKMO with all maturity classes, while change of ripening period from heading to harvesting date was different with maturity classes. That is, physical maturity was shortened by 1~3 days for early maturity class and 14~18 days for late maturity class under different climate change scenarios. Rice yield was in general reduced by 4.5%, 8.2%, 9.9%, and 14.9% under the climate in $2^{\circ}C$, $3^{\circ}C$, $4^{\circ}C$, and about $5^{\circ}C$ increase, respectively. The yield reduction was due to increased high temperature-induced spikelet sterility and decreased growth period. The results show that predicted climate changes are expected to bring negative effects in rice production in Korea. So, it is required for introduction of new agricultural technologies to adapt to climate change, which are, for example, developing new cultivars, alternations of planting dates and management practices, and introducing irrigation systems, etc.