• Title/Summary/Keyword: Internal transcribed spacer region

Search Result 344, Processing Time 0.026 seconds

Comparison of ITS(Internal Transcribed Spacer) and 5.8S rDNA Sequences among varieties and Cultivars in Panax ginseng

  • Yang, Deok-Chun;Yang, Key-Jin;Yoon, Eui-Soo
    • Journal of Photoscience
    • /
    • v.8 no.2
    • /
    • pp.55-60
    • /
    • 2001
  • Ginseng (Panax genus) is one of the most medicinally important genera and consists of highly regarded medicines. Among the species of Panax, the ginseng species is widely known to have most medicinal quality. P. ginseng has 3 varieties, Jakyung, Chunggyung and Hwangsook, discovered in nature with different colors of stem and fruit, Jakyung has two cultivars, Yunpoong and Chunpoong. Rigorous phylogenetic analysis of these varieties and cultivars has been conducted with sequencing of rDNA region. The sequences of ITS1, ITS2 of every varieties and cultivars within P. ginseng were identical. The sequence of 5.8S rDNAs of Hwangsook variety were different from the sequences of 5.8S rDNAs of others by only one base pair at nucleotide position 14. In phylogenetic analysis and predicted RNA secondary structure study, it is assumed that evolution has proceeded from Hwangsook to other varieties. recently.

  • PDF

Four Unrecorded Wood Decay Fungi from Seoul in Korea

  • Jang, Yeong-Seon;Lee, Sung-Wook;Jang, Seok-Yoon;Lim, Young-Woon;Lee, Jin-Sung;Kim, Jae-Jin
    • Mycobiology
    • /
    • v.40 no.3
    • /
    • pp.195-201
    • /
    • 2012
  • During the investigation of Korean indigenous fungi from Seoul, three genera-Fuscoporia, Porostereum, and Trametopsis, and four species-Fuscoporia senex, Phlebia acerina, Porostereum spadiceum, and Trametopsis cervina were found. Their morphological characteristics were examined and their identification was confirmed by molecular analysis based on internal transcribed spacer (ITS) and nuclear large subunit ribosomal DNA region sequences. These fungi are new to Korea and registered here with descriptions.

Detection of Plasmodiophora brassicae by Using Polymerase Chain Reaction (PCR을 이용한 Plasmodiophora brassicae의 검출)

  • 지희윤;김완규;조원대;지형진;최용철
    • Korean Journal Plant Pathology
    • /
    • v.14 no.6
    • /
    • pp.589-593
    • /
    • 1998
  • DNA amplification by polymerase chain reaction (PCR) was used to specifically detect Plasmodiophora brassicae, causing clubroot of crucifers. On the basis of DNA sequence informations, an oligonucleotide primer set specific for the pathogen was designed form small subunit gene (18S-like) and internal transcribed spacer (ITS) region of ribosomal DNA. Primer ITS 5/PB-C produced an amplification product of approximately 520 bp in length with DNA from P. brassicae. However, no amplification product was produced with DNAs from several soil-borne fungi, Didymella bryoniae and Rhizopus stolonifer. Using these primers, the clubroot pathogen was readily detected from infected roots of crucifers, but not from healthy roots. Southern hybridization analysis further confirmed that the amplification product was originated from P. brassicae.

  • PDF

Identification of Aspergillus Strain with Antifungal Activity Against Phytophthora Species

  • KANG SUNG WOO;HONG SUK IN;KIM SEUNG WOOK
    • Journal of Microbiology and Biotechnology
    • /
    • v.15 no.2
    • /
    • pp.227-233
    • /
    • 2005
  • Fungal strain CGF was isolated from the soil of ChungNam Province, South Korea. Based on the 28S rDNA sequence analysis and the sequence of the internal transcribed spacer (ITS) region of ribosomal DNA, together with morphological and cultural characteristics, this strain was identified as Aspergillus sclerotiorum and renamed Aspergillus sclerotiorum CGF. This is the first strain of Aspergillus sclerotiorum identified in Korea. When the antifungal activity of A. sclerotiorum CGF was evaluated, among the phytopathogenic fungi, mycelial growth of only Phytophthora species was inhibited. Oermination of P. capsid zoospore was also inhibited. The bioactive compound of A. sclerotiorum CGF was highly thermo- and pH-stable.

A Note on a Dark Septate Endophyte Phialocephala piceae Isolated from Needle Leaves of Thuja koraiensis in Korea

  • Kim, Dong-Yeo;Eo, Ju-Kyeong;Park, Hyeok;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.44 no.4
    • /
    • pp.338-341
    • /
    • 2016
  • A dark septate endophytic fungal strain 13E043 was isolated from the needle leaves of Thuja koraiensis from a forest in Korea. Morphological characteristics of conidia and phialids. Along with a molecular phylogenetic analysis based on the internal transcribed spacer region of rDNA, the isolate was identified as Phialocephala piceae (also known as Phaeomollisia piceae). This is the first report of a dark septate endophyte isolated from the foliage of conifer trees in Korea.

Four Species of Montagnulaceae Unrecorded in Korea and Isolated from Plant Litter in Freshwater

  • Goh, Jaeduk;Mun, Hye Yeon;Oh, Yoosun;Chung, Namil
    • The Korean Journal of Mycology
    • /
    • v.44 no.4
    • /
    • pp.263-270
    • /
    • 2016
  • Freshwater is a diverse and complex environment for fungi and provides several types of habitat including plant litter, sediment, and carcasses. We collected plant litter from the main stream and branch streams of Nakdong River, Sohancheon in Samcheok, and Geumoreum in Jeju. From several samples of plant litter, we isolated 8 fungal strains belonging to 4 Montagnulaceae species unrecorded in Korea: Paraconiothyrium archidendri, Paraphaeosphaeria sporulosa, Paraphaeosphaeria michotii, and Paraphaeosphaeria viridescens. These fungi were identified by phylogenetic analysis using the internal transcribed spacer (ITS) region and examination of morphological characteristics. Montagnulaceae is known as one of the families in Pleosporales and includes coniothyrium-like fungi. In this study, we described phylogenetic analysis and mycological characteristics of these species, and this is the first report of these taxa in Korea.

Characterization of a Septobasidium sp. Associated with Felt Disease of Schisandra chinensis

  • Choi, In-Young;Lee, Wang-Hyu;Lee, Jong-Jin;Park, Mi-Jeong;Ko, Jeong-Ae;Choi, Jeong-Ran;Shin, Hyeon-Dong
    • Mycobiology
    • /
    • v.44 no.1
    • /
    • pp.58-62
    • /
    • 2016
  • Extensive disease surveys performed during the summers of 2013 and 2014 in Schisandra chinensis orchards resulted in the finding of a Septobasidium sp. associated with felt disease. The fungus was characterized to be symbiotic with a scale insect (Pseudaulacaspis cockerelli). Morphological and molecular characteristics of the Septobasidium isolates were investigated. The isolates were morphologically and phylogenetically close to S. bogoriense. We tentatively describe this isolate as a Septobasidium sp., mainly because of the limited amount of information available on the internal transcribed spacer region of the ribosomal DNA of Septobasidium spp.

Fungal Endophytes Isolated from Needle Leaves of Three Coniferous Species on Mt. Seodae of Korea

  • Eo, Ju-Kyeong;Kim, Chang-Kyun;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.43 no.2
    • /
    • pp.133-136
    • /
    • 2015
  • We investigated endophytic fungi diversity of two Pinaceae species (Pinus densiflora, Pinus rigida) and one Cupressaceae species (Juniperus rigida) on Mt. Seodae, Korea. In total, 34 isolates were obtained from 19 host plants and identified according to internal transcribed spacer (ITS) region. As a result, they were placed in 13 taxa. Among them, 52.9% belonged to Leotiomycetes, 32.3% belonged to Sordariomycetes, 5.8% belonged to Agaricomycetes, and 3.0% for isolates belonged to either Eurotiomycetes, Dothideomycetes or Ustilaginomycetes. In particular, Lophodermium growing in pine would be an especially important instance of biodiversity for endophytes, suggesting that further study examining its ecological function in the environment is critical.

A New Report on Oidiodendron flavum Isolated from Field Soil in Korea

  • Adhikari, Mahesh;Kim, Sangwoo;Yadav, Dil Raj;Babu, Anam Giridhar;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • The Korean Journal of Mycology
    • /
    • v.42 no.3
    • /
    • pp.235-238
    • /
    • 2014
  • Oidiodendron flavum KNU13-6 was isolated for the first time from field soil in Korea and identified based on the internal transcribed spacer region (ITS) of rDNA and morphological characteristics. Based on phylogenetic analysis of ITS and morphological characteristics, the species has not been previously reported in Korea.

Monotropoid Mycorrhizal Characteristics of Monotropa uniflora (Ericaceae) Collected from a Forest in Korea

  • Lee, Eun-Hwa;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.42 no.3
    • /
    • pp.243-246
    • /
    • 2014
  • The roots of Monotropa uniflora were collected from a forest in Korea. Morphological characteristics of monotropid mycorrhizas of the plants were determined. Thick mantles covered the roots and fungal pegs inside the epidermal cells of the roots were observed. Fungal symbionts were identified by sequence analysis of internal transcribed spacer region. Phylogenetic analysis based on the sequences demonstrated that the fungus was the most closely related to Russula heterophylla. The result support the strong specificity between M. uniflora and Russula species.