• Title/Summary/Keyword: Intermediate products

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Synthesis of New Uracil-5-Sulfonamide Derivatives and Immuno-Stimulatory Effect of a Chemically Modified Hemolymph of Biomphalaria alexandrina on Schistosoma mansoni Infected Mice

  • Fathalla, O.A.;Haiba, M.E.;Maghraby, A.S.
    • Archives of Pharmacal Research
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    • v.26 no.5
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    • pp.358-366
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    • 2003
  • Some N-p-substituted phenyl uracil-5-sulphonamide derivatives have been synthesized to be evaluated as molluscicides against Biomphalaria alexandrina snails, the intermediate host of Schistosoma mansoni. Schistosoma mansoni infected mice were treated with hemolymph obtained from pre-treated Biomphalaria alexandrina snails with the products 4a, 10a, 10b and 4b or obtained from non-treated snails. The selection of the concentration based on the predetermined dose which caused mortality of less than 50% of snails/24 h. $LC_{50}$ of compounds 4a, 10a, 10b and 4b was 50, 100, 200 and 50 ppm respectively. The result showed that immuno-stimulatory effect of treated hemolymph with compounds 4a, 10a and 4b was related to significant protective effects (44.4, 34.6 and 50.4% reduction in worm burden respectively). In addition, mean total worm burdens were significantly reduced in non treated hemolymph by 33.8%. The effect of hemolymph obtained from treated or non treated snails on S. mansoni adult worms antigens was studied by indirect immunofluorescence technique using chronic mouse sera (CMS). The results indicated that there was a strong reaction with epitopes in gut epithelium, tubercles, teigument and subtegumental musculature of untreated and treated S. mansoni adult worms antigens. Therefore, treatment of hemolymph obtained from pre-treated snails with compounds 4a, 10a, and 4b can stimulate specific immune response and induce protective effects against S. mansoni infection.

Bi-stability in a vertically excited rectangular tank with finite liquid depth

  • Spandonidis, Christos C.;Spyrou, Kostas J.
    • Ocean Systems Engineering
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    • v.2 no.3
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    • pp.229-238
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    • 2012
  • We discuss the bi - stability that is possibly exhibited by a liquid free surface in a parametrically - driven two-dimensional (2D) rectangular tank with finite liquid depth. Following the method of adaptive mode ordering, assuming two dominant modes and retaining polynomial nonlinearities up to third-order, a nonlinear finite-dimensional nonlinear modal system approximation is obtained. A "continuation method" of nonlinear dynamics is then used in order to elicit efficiently the instability boundary in parameters' space and to predict how steady surface elevation changes as the frequency and/or the amplitude of excitation are varied. Results are compared against those of the linear version of the system (that is a Mathieu-type model) and furthermore, against an intermediate model also derived with formal mode ordering, that is based on a second - order ordinary differential equation having nonlinearities due to products of elevation with elevation velocity or acceleration. The investigation verifies that, in parameters space, there must be a region, inside the quiescent region, where liquid surface instability is exhibited. There, behaviour depends on initial conditions and a wave form would be realised only if the free surface was substantially disturbed initially.

Extracellular Production of Alpha-Interferon by Recombinant Escherichia coli : Part I. Construction of Expression Vectors (유전자 재조합 대장균을 사용한 Alpha-interferon의 생산과 분비: 제 1 부. 발현벡터의 제작)

  • 노갑수;최차용
    • KSBB Journal
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    • v.5 no.1
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    • pp.49-58
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    • 1990
  • We constructed hybrid plasmids to allow controlled and extracellular production of human alpha-interferon in Escherichia coli. The hybrid plassmids were constructed by transferring alpha-lFN gene from plasmid Hif-2h which has the alpha-lFN gene at PstI restriction site of pBR322, to plasmids pIN -IIIB3 and pIN-IIIC3 at restriction sites between HindIII and BamHI. Plasmids pIN-IIIB3 and pIN-IIIC3 carry E. coli lipoprotein promoter, lac promoter and operator in tandem. The plasmids also have lacl genes which encode for lac repressors, which allows controlled expression of genes cloned to the plasmids by using of inducer IPTG. Lipoprotein signal sequence is located just ahead of cloning sites of the plasmids, which helps cells to excrete or secrete cloned gene products. Plasmid pUC9 was used as a intermediate vector for transferring of alpha-lFN gene from Hif-2h to pIN vectors in order to solve the problem of different restriction sites between Hif-2h and pIN vectors.

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A Study on the Physico-Chemical Property Evaluation of Oxidative Permanent Hair Color Products Containing p-Phenylene Diamine (파라-페닐렌디아민이 함유(含有)된 산화형(酸化形) 영구염모제(永久染毛制)의 물리화학적(物理化學的) 특성평가(特性評價)에 관(關)한 연구(硏究))

  • Ha, Byung-Jo;Jeon, Dong-Won;Kim, Kyung-Sun
    • Journal of Fashion Business
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    • v.9 no.5
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    • pp.136-144
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    • 2005
  • Oxidative hair color is currently widely used because of its beautiful light color and good dyeability. In this study, hair color, oxidative agents, and dye intermediate were reacted. Modifier, which changes color with various hue, and alkaline agent, which opens the cuticle of hair fiber and enhances the dyeing reaction, were added. For gel formation, isocetyl alcohol was used as solvent in preparing three kinds of permanent oxidative hair color. Physiochemical study of prepared permanent oxidative hair color was done for basic information acquisition. Acid perspiration test, pH measurement both at room temperature and at high temperature, drop movement test, dyeability efficiency upon the amount of hydrogen peroxide added, and humidity fastness were studied. Shampoo fastness and light fastness of prepared permanent oxidative hair color dyed hair sample were also investigated. All three kinds of hair color prepared showed good properties overall, however, dyed hair sample became reddish upon shampoo fastness and lightfastness tests.

Study on Decomposition of Several Pesticides by Gamma-Irradiation (감마선 조사에 의한 농약 성분의 분해 연구)

  • Lee, Joo-Sang;Jung, Su-Yeon;Oh, Seung-Yeol;Jang, Beom-Su;Park, Sang Hyun
    • Journal of Radiation Industry
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    • v.6 no.3
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    • pp.205-209
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    • 2012
  • The decomposition of 50 pesticides present in an aqueous solution using ${\gamma}-irradiation$ from a $^{60}Co$ gamma-ray source was investigated using laboratory-scale experiment. The rates of decomposition were determined using a gas chromatography-electron capture detector (GC-ECD), high-performance liquid chromatography-photo diode array detector (HPLC-PDA), and HPLC-fluorescence detector (FLD). When the initial concentration of pesticides was 10 ppm, and the radiation dose was 2, 5, 10, 20, and 30 kGy, respectively, 14 pesticide samples showed high removal rates (>50%) at absorbed doses of more than 10 kGy. With the exception of procymidone, they were all completely removed at a 30 kGy irradiation dose. These results provide fundamental data on the reactivity between gamma-irradiation and pesticides in an aqueous solution. Further, an evaluation of the toxicity of radiolytic intermediate products is required.

Integrated Thermochemical Approach to Collision-Induced Dissociation Process of Peptides

  • Shin, Seung Koo;Yoon, Hye-Joo
    • Mass Spectrometry Letters
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    • v.12 no.4
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    • pp.131-136
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    • 2021
  • Collision-induced dissociation of peptides involves a series of proton-transfer reactions in the activated peptide. To describe the kinetics of energy-variable dissociation, we considered the heat capacity of the peptide and the Marcus-theory-type proton-transfer rate. The peptide ion was activated to the high internal energy states by collision with a target gas in the collision cell. The mobile proton in the activated peptide then migrated from the most stable site to the amide oxygen and subsequently to the amide nitrogen (N-protonated) of the peptide bond to be broken. The N-protonated intermediate proceeded to the product-like complex that dissociated to products. Previous studies have suggested that the proton-transfer equilibria in the activated peptide affect the dissociation kinetics. To take the extent of collisional activation into account, we assumed a soft-sphere collision model, where the relative collision energy was fully available to the internal excitation of a collision complex. In addition, we employed a Marcus-theory-type rate equation to account for the proton-transfer equilibria. Herein, we present results from the integrated thermochemical approach using a tryptic peptide of ubiquitin.

Factors Affecting Consumer Purchasing Behavior: A Green Marketing Perspective in Vietnam

  • LE, Quang Hung
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.5
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    • pp.433-444
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    • 2021
  • The study seeks to identify the factors affecting the green marketing element of students' food purchasing decision at Co-opMart supermarket chain in Ho Chi Minh City through the application of a mix of qualitative and quantitative research methods that include probability sampling and convenient sampling of 400 students from Ho Chi Minh City University of Technology (HUTECH). The data are analyzed with SPSS software using Cronbach's Alpha, Exploratory Factor Analysis, Multiple Linear Regression and PATH model to test the model through the intermediate variable 'student's perception' and the hypotheses, identifying the green marketing effects on HUTECH students' food purchasing decisions at Ho Chi Minh City Co-opMart supermarket chain. The results of the study identify four factors of the green marketing mix (4Cs), namely, green commodity, green cost, green convenience, and green communication. All these factors have an influence on the student's food purchasing decision at Co-opMart supermarket. Cost is the strongest factor eliciting student's interest in purchasing green products, followed by convenience, then communication. Commodity has the least impact on green purchasing decision. This study proposes some feasible solutions for Co-opMart managers to attract more students using green food in the complex situation of contaminated food, which is extremely harmful to consumers' health.

Oxygen Control in CdS Thin Film by UV Illumination in Chemical Bath Deposition (용액성장법에서 자외선 조사를 이용한 CdS의 산소함량 제어)

  • Baek, Hyeon-ji;Oh, Ji-A;Seo, Young-Eun;Shin, Hye-Jin;Cho, Sung-Wook;Jeon, Chan-Wook
    • Current Photovoltaic Research
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    • v.7 no.2
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    • pp.33-37
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    • 2019
  • In this paper, we compared the performance of $Cu(In,Ga)(S,Se)_2$ (CIGSSe) thin film solar cell with CdS buffer layer deposited by irradiating 365 nm UV light with 8 W power in Chemcial Bath Deposition (CBD) process. The effects of UV light irradiation on the thin film deposition mechanism during CBD-CdS thin film deposition were investigated through chemical and electro-optical studies. If the UV light is irradiated during the solution process, the hydrolysis of Thiourea is promoted even during the same time, thereby inhibiting the formation of the intermediate products developed in the reaction pathway and decreasing the pH of the solution. As a result, it is suggested that the efficiency of the CdS/CIGSSe solar cell is increased because the ratio of the S element in the CdS thin film increases and the proportion of the O element decreases. This is a very simple and effective approach to control the S/O ratio of the CdS thin film by the CBD process without artificially controlling the process temperature, solution pH or concentration.

Degradation characteristics and reaction pathways of tetracycline by ferrate(VI) in various aqueous conditions (Ferrate(VI)를 이용한 다양한 수중 환경에서의 tetracycline의 분해 특성 및 반응 경로 연구)

  • Park, Kyeong-Deok;Kim, Il-Kyu
    • Journal of Korean Society of Water and Wastewater
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    • v.35 no.1
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    • pp.27-37
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    • 2021
  • Tetracycline is one of the most commonly used as antibiotics for the livestock industry and it is still widely used nowadays. Tetracycline and its metabolites are excreted with excrement, which is difficult to completely removed with conventional sewage treatment, therefore it is apprehended that the tetracycline-resistant bacteria occurs. In this study, the oxidant named ferrate(VI) was used to degrade the tetracycline and investigate the reaction between ferrate(VI) and tetracycline under various aqueous conditions. The highest degradation efficiency of tetracycline occurred in basic condition (pH 10.1 ± 0.1) because of the pKa values of tetracycline and ferrate(VI). The results also showed the effect of water temperature on the degradation of tetracycline was not significant. In addition, the dosage of ferrate(VI) was higher, the degradation of tetracycline and the self-degradation of ferrate(VI) also higher, finally the efficiency of ferrate(VI) was lower. The results said that the various mechanisms effects the reaction of ferrate(VI) oxidation, it required the consideration of the characteristics of the target compound for optimal degradation efficiency. Additionally, intermediate products were detected with LC/MS/MS and three degradation pathways were proposed.

Simulative Calculations of Food Waste Reduction Using Kineto-transport Models (동력학-전달 모델을 활용한 식품 폐기물 감량 해석)

  • Cho, Sun-joo;Kim, Tae-wook;Kwon, Sung-hyun;Cho, Daechul
    • Journal of Environmental Science International
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    • v.30 no.6
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    • pp.429-439
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    • 2021
  • Food waste is both an industrial and residential source of pollution, and there has been a great need for food waste reduction. As a preliminary step in this study, waste reduction is quantitatively modeled. This study presents two models based on kinetics: a simple kinetic model and a mass transport-shrinking model. In the simple kinetic model, the smaller is the reaction rate constant ratio k1, the lower the rate of conversion from the raw material to intermediate products. Accordingly, the total elapsed reaction time becomes shorter. In the mass transport-shrinking model, the smaller is the microbial decomposition resistance versus the liquid mass transfer resistance, the greater is the reduction rate of the radius of spherical waste particles. Results showed that the computed reduction of waste mass in the second model agreed reasonably with that obtained from a few experimantal trials of biodegradation, in which the microbial effect appeared to dominate. All calculations were performed using MATLAB 2020 on PC.