• Journal of Microbiology and Biotechnology
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• v.10 no.6
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• pp.789-796
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• 2000

In order to analyze the effects of tktA, $aroF^{FBR}$, and aroL expression in a tryptophan-producing Escherichia coli, a series of plasmids carrying the genes were constructed. Introduction of tktA, $aroF^{FBR}$, and aroL into the E. coli strain resulted in approximately 10-20 fold increase in the activities of transketolase, the feedback inhibition-resistant 3-deoxy-D-arabinoheptulsonate-7-phosphate synthase, and shikimate kinase. Expression of $aroF^{FBR}$ in the aroB mutant strain of E. coli resulted in the accumulation of 10 mM of 3-deoxy-D-arabinoheptulsonate-7-phosphate (DAHP) in the medium. Simultaneous expression of tktA and $aroF^{FBR}$ in the strain further increased the amount of excreted DAHP to 20 mM. In contrast, the mutant strain which has no gene introduced accumulated 0.5 mM of DAHP. However, the expression of tktA and $aroF^{FBR}$ in a tryptophan-producing E. coli strain did not lead to the increased production of tryptophan, but instead, a significant amount of shikimate, which is an intermediate in the tryptophan biosynthetic pathway, was excreted to the growth medium. Despite the fact that additional expression of shikimate kinase in the strain could possibly remove 90% of excreted shikimate to 0.1 mM, the amount of tryptophan produced was still unchanged. Removing shikimate using a cloned aroL gene caused the excretion of glutamate, which suggests disturbed central carbon metabolism. However, when cultivated in a complex medium, the strain expressing tktA, $aroF^{FBR}$, and aroL produced more tryptophan than the parental strain. These data indicate that additional rate-limiting steps are present in the tryptophan biosynthetic pathway, and the carbon flow to the terminal pathway is strictly regulated. Expressing tktA in E. coli cells appeared to impose a great metabolic burden to the cells as evidenced by retarded cell growth in the defined medium. Recombinant E. coli strains harboring plasmids which carry the tktA gene showed a tendency to segregate their plasmids almost completely within 24h.

• Journal of KIISE
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• v.41 no.11
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• pp.892-899
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• 2014

Dynamic binary instrumentation is a code insertion technique for debugging a program without scattering its execution flow, while the program is running. Most dynamic instrumentations are implemented using dynamic binary translation techniques. Existing studies translated program codes dynamically by parsing the machine code stream to intermediate representation (IR) and then applying compilation techniques for IRs. However, they have high overhead during translation, which is a major cause of difficulty in applying the dynamic binary translation technique to the program which requires high responsiveness. In this paper, we introduce a light-weight dynamic binary instrumentation framework based on a novel dynamic binary translation technique which has low overhead while translating the program code. In order to reduce the translation overhead, our approach adopts a tabular-based address translation and exploits a translation bypassing scheme, which stores the translated address of a frequently called library function in advance. It then accesses the translated address and executes function codes without code translation when calling the function. Our experiment results demonstrated that the proposed approach outperforms the prior dynamic binary translation techniques from 2% up to 65%.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.5
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• pp.543-547
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• 2015

The cooling process referred to as Tempcore has been applied to produce a high-strength rebar. Excellent rebar with strength and weldability can be manufactured from mild steel without the addition of alloying elements by using the Tempcore process. However, there are limitations to evaluating the effect of various chemical compositions and cooling conditions within a site facility. In this study, we developed an apparatus to simulate the Tempcore process and obtained microstructures with a hardened surface layer, an intermediate region and a soft inner core. The experimental apparatus has been equipped with a cooler set that is the same as the site facility and consists of a pump line that supplies pressure of 12-13 bar and flow rate of up to $50m^3/h$. In accordance with the simulation result of steel grade SD400 that requires more than 400 MPa of yield strength, both the hardened area ratio and the hardness with respect to each cooling depth were found to agree well with the product.

• The Korean Journal of Cytopathology
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• v.7 no.1
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• pp.1-11
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• 1996

From the concepts of cellular pathology and of exfoliative cytology, as elucidated by Virchow and Papanicolaou respectively in the late 19th and early 20th century, have evolved the primary methods for the diagnosis of cancer today. From Papanicolaou's concept of exfoliative cytology developed fine needle aspiration biopsy in the early 1960's, this has become a major diagnostic procedure and has contributed to a significant reduction in open biopsies and, therefore, to medical cost-effectiveness immunobiochemical techniques provided us with a supplement to cancer diagnosis in the 1980's. The immunoperoxidase method, using monoclonal antibodies, is applied primarily as an ancillary measure to elucidate the nature of cancers The availability of specific monoclonal antibodies has greatly facilitated the identification of cell products or surface markers. For example, antibodies directed against intermediate filaments have proved to be of value in determining the histogenesis oi poorly differentiated neoplasms. Tumor markers may serve as biochemical indicators of the presence of a neoplasm. They can be detected In plasma and other body fluids. Their concentration can be applied as a diagnostic test, for monitoring the clinical course of known cancer, and as a screening measure to detect certain cancers in a population at risk. Flow cytometry is a useful tool for distinguishing several cell characteristics, such as the immunophenotype of leukemia-lymphoma cells, the DNA content of neoplastic cells, and cell proliferation rate. Molecular biologic techniques provided a giant step for the management of cancer patients encompassing diagnosis, prognostic evaluation, and therapy. Nucleic acid hybridization techniques are utilized as Southern, Northern, and dot blots and in situ hybridization. Molecular biology and its techniques may bring a blight new horizon for understanding cancer biology and in designing therapy on the basis of gene manipulation.

• Journal of Korean Tunnelling and Underground Space Association
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• v.14 no.4
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• pp.293-308
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• 2012

The shear behavior at the particle/surface interface such as rock joint can determine the mechanical behavior of whole structure. Therefore, a fundamental understanding of the mechanisms governing its behavior and accurately estimation of the interface strength is essential. In this paper, PFC, a numerical analysis program of discrete element method was used to investigate the effects of the surface roughness on interface strength. The surface roughness was characterized by smooth, intermediate, and rough surface, respectively. In order to investigate the effects of particle shape and crushing on particle/surface interface behavior, one ball, clump, and cluster models were created and their results were compared. The shape of particle was characterized by circle, triangle, square, and rectangle, respectively. The results showed that as the surface roughness increases, interface strength and friction angle increase and the void ratio increases. The one ball model with smooth surface shows lower interface strength and friction angle than the clump model with irregular surface. In addition, a cluster model has less interface strength and friction angle than the clump model. The failure envelope of the cluster model shows non-linear characteristic. From these findings, it is verified that the surface roughness and particle shape effect on the particle/surface interface shear behavior.

• Journal of Korean Tunnelling and Underground Space Association
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• v.14 no.4
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• pp.321-336
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• 2012

The shear behavior at the particle/surface interface such as rock joint can determine the mechanical behavior of whole structure. Therefore, a fundamental understanding of the mechanisms governing its behavior and accurately estimation of the interface strength is essential. In this paper, PFC, a numerical analysis program of discrete element method was used to investigate the effects of the surface roughness crushing on interface strength. The surface roughness was characterized by smooth, intermediate, and rough surface, respectively. Particle shape was classified into one ball model of circular shape and 3 ball model of triangular shape. The surface shape was modelled by wall model of non-crushing surface and ball model of crushing surface. The results showed that as the bonding strength of ball model decreases, lower interface strength is induced. After the surface roughness crushing was occurred, the interface strength tended to converge and higher bonding strength induced lower surface roughness crushing. Higher friction angle was induced in wall model and higher surface roughness induced the higher friction angle. From these findings, it is verified that the surface roughness and surface roughness crushing effect on the particle/surface interface shear behavior.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.6
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• pp.549-556
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• 1984

Forty-eight fish species belonging to 38 genera and 10 families were collected along the Dongjin River from July 1982 to May 1984. The dominant species of them were Rhinogobius brunneus and Zacco platypus in the main stream and Abbottina springeri, Hemiculter eigenmanni and Moroco oxycephalus in the tributaries respectively. It was remarkable point in the distributional study of freshwater-fishes of Korea that both species of Pelteobagrus nitidus and Lefua costata were observed in the present studied areas for the first time. It was found that 6 species of Hypomesus olidus, Hemiculter eigenmanni, Pseudobagrus sp., Liobagrus mediadiposalis, Cobitis taenia striata, and Lepomis macrochirus inhabiting the seomjin River were unstably incorporated into the fish communities at the upper reaches of the Dongjin River which the Seomjin River flow down for the water power generation. It is suggested that the integradation between two subspecies of C. taenia occurs at the intermediate water areas in the mainstream of the Dongjin River, whereas C. t. striate is remained only at the upper reaches and C. t. lutheri is lived in the lower parts of the river. Population of C. koreensis found in the mainstream was exhibited considerable differences in the color patterns on the body sides of them in comparison with other populations, which was discussed in relation to the habitat variations adapted to the confluent condition of joining the Seomjin stream with the Dongjin River.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.10
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• pp.1809-1816
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• 2000

The catalytic combustion of methanol as a model volatile organic compound(VOC) was been investigated over metal-phthalocyanine(PC) in a fixed bed flow reactor system. The catalytic activity of Co-PC pretreated with air and methanol mixture at $450^{\circ}C$ and 60 cc/min for 1 hr was very excellent. The order of catalytic activity on methanol combustion was summarized as follows: metal free-PC < Zn-PC < Fe-PC < Cu($\alpha$)-PC < Co-PC. By TG/DTA analysis, the tendency of thermal decomposition was increased as follows: metal free-PC < Zn-PC < Cu($\alpha$)-PC < Co-PC < Fe-PC. Under this pretreatment condition, the basic structures of Co-PC, Cu($\alpha$)-PC and Fe-PC were destroyed, and the new metal oxide such as $Co_3O_4$ from Co-PC was confirmed by EA and XRD analysis. But Zn-PC and metal free-PC were retained its basic structure under this pretreatment condition. On the combustion of methanol over Co-PC, HCHO and $HCOOCH_3$ were observed as an intermediate products in the high concentration of reactant or the short contact time(W/F).

• The Journal of Engineering Research
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• v.6 no.1
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• pp.97-109
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• 2004

The synthesis and crystal structure of amorphous calcium carbonate obtained from gas-liquid reaction of CaO-$C_2 H_5 OH$-$CO_2$ system according to change of added amount of calcium oxide by blowing $CO_2$ gas and reaction time using ethanol and ethylene glycol were investigated by electric conductivity, X-ray diffraction, and scanning electron microscope. The powdery or gelatinous phases were prepared by passing $CO_2$ gas at a flow rate of 1$\ell$/min into the suspensions containing 10~40g of CaO in mixing solutions 900ml of $C_2 H_5 OH$- and 100ml of ethylene glycol. By rapid filtration and drying the both phases at $60^{\circ}C$ under reduced pressure, the phases converted to the spherical vaterite and amorphous phase. The stable phase of amorphous calcium carbonate(ACC) was formed in the region pH 7-9 but the formation regions of amorphous phase were remarkably affected by pH in the mother liquor. It seems that a part of ACC changed into chain calcite as an intermediate products. The initial reactants prior to the formation of precipitated calcium carbonate is ACC. And ACC is unstable in the aqueous solution and crystallizes finally to calcite by the through-solution reaction. Especially ACC was produced or gelatinous phase which precipitated from the reaction of CaO-$C_2 H_5 OH$-$CO_2$ system.

• Journal of Chest Surgery
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• v.28 no.6
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• pp.571-578
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• 1995

Rastelli operation in which right ventricle[RV and pulmonary artery[PA is connected with an artificial graft is effective in increasing the pulmonary blood flow in certain types of congenital heart disease but, in many, it requires a reoperation because of the relative stenosis of graft that develops as the patients become old. The purpose of this study is to evaluate the various factors which many influence the long term outcome of such patients following a Rastelli operation. A total of 47 patients underwent a Rastelli operation during a 15 year period between November, 1978 and October 1993. The mean follow-up period is 76.1 51.3 months.1 Among the 47 patients, a valved conduit was used in 30[63.8% , and non-valved conduit in 17[36.2% patients. In the 8 patients[17.0% who died postoperatively, a valved conduit was used in 5 [16.6% and a non-valved conduit in 3[17.6% . There was no statistical difference in mortality between the 2 groups. There was a good linear correlation between the body surface area[X and the conduit size[Y [Y=3.86X + 14.6, R=0.55, P=0.01 .2 Ten patients underwent replacement of the conduit during the follow-up period. The type of conduit used and the frequency of subsequent replacement were as follows: Ionescu-Shiley, valved-33.3%, Carpentier-Edwards, valved-30.8%, Hancock, valved-80% and non-valved conduit-9.1%. The median period free of reoperation was 110 months for the valved and 79 months for the non-valved group, there being no statistical difference between the 2 groups. 3 The patients who did not require reoperation are all doing well [New York Heart Association Functional Classification: Class I . Pressure gradient between the RV and the PA was 20 mmHg in 10 randomly selected patients who did not require reoperation and 92 9 mmHg in 10 patients who did require reoperation.4 In the 10 patients who underwent a conduit replacement procedure.5 Among patients undergoing reoperation, 2 died from endocarditis.The remaining 8 patients are doing well without limitation in physical activity at a mean follow-up period of 32.7 33.9 months [range 2 to 89 months . 6 At 5, 7, and 10 years, the reoperation-free rates among all patients were 96%, 91% and 29% and the survival rates were 82%, 82% and 71%. In conclusion, Rastelli operation is an effective procedure in ameliorating symptoms in a select group of patients with congenital heart disease. Because of the inherent nature of relative graft stenosis and degeneration, a long-term follow-up is required under the proper selection of the graft material.