• Journal of Genetic Medicine
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• 제9권2호
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• pp.73-77
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• 2012

Purpose: Fragile X carrier detection before or at early pregnancy through a wide screening program may not only confer a risk of having offspring with Fragile X syndrome (FXS), but may also confer a risk for Fragile X-associated primary ovarian insufficiency and Fragile X-associated tremor/ataxia syndrome. However, prior to the implementation of such a program, the carrier prevalence in a population and the availability of effective screening test should be evaluated. The aim of our study was to determine the prevalence of premutation carriers and to evaluate the feasibility of screening test. Materials and Methods: The blood samples were obtained from 8,641 pregnant women with no family history of mental retardation. We performed a three-primer CGG repeat primed (RP) PCR using the AmplideX$^{TM}$ FMR1 PCR kit (Asuragen, Inc. Austin, TX, USA). Samples showing full mutation alleles were reflexed to Southern blot analysis for methylation status and sizing. Results: Among the 8,641 women, we found 8 premutation carriers (1:1,090, 0.09%) and 46 women with an intermediate allele (1:190, 0.53%). No woman was found to carry the fully mutated allele. All the detected alleles were within the CGG repeat range of 8-117. Among the 8,641 samples, 29 and 30 CGG repeats represent 66.6% of all cases. The CGG RP PCR method provides robust detection of expanded alleles and resolves allele zygosity, thus minimizing the number of samples that require Southern blot analysis. Conclusion: This is the first study that has focused on the prevalence of FXS premutation carriers and FMR1 allele distribution in normal pregnant women. These data have important implications for population-based fragile X carrier screening in Korea.

• Asian-Australasian Journal of Animal Sciences
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• 제20권11호
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• pp.1738-1745
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• 2007

A growth trial and a digestibility trial were conducted to examine the effect of feed particle size on the performance, nutrient digestibility, gastric ulceration and intestinal morphology in pigs fed barley-based diets. Barley was processed through a hammer mill to achieve four diets varying in particle size (average particle $size{\pm}standard $deviation): coarse ($1,100{\pm}2.19\;{\mu}m$), medium ($785{\pm}2.23\;{\mu}m$), fine ($434{\pm}1.70\;{\mu}m$) and mixed (1/3 of coarse, medium and fine) ($789{\pm}2.45\;{\mu}m$). Sixty-four entire male pigs were used in the growth trial and the diets were fed ad libitum between 31 kg and 87 kg live weight. Following slaughter, stomach and ileal tissues were scored for integrity (ulceration or damage) and histological measurements taken. Twenty-four entire male pigs were used in the digestibility trial, which involved total faecal collection. Over the entire growth phase, there were no differences (p>0.05) in average daily gain and feed conversion ratio between pigs fed diets of different particle size. Pigs fed the coarse and medium diets had lower (p<0.05) stomach ulceration scores (0.20 and 0.25, respectively, on a scale from 0 to 3) than those fed the mixed (0.69) or the fine diets (1.87). The stomachs of all animals fed the fine diet had lesions and stomach ulcerations were present only in this group. Pigs fed the fine diet had thicker (p<0.001) ileal epithelial cell layer with no differences (p>0.05) being observed for villous height or crypt depth. Faecal digestibility coefficients of neutral and acid detergent fibre were the highest (p<0.05) for the mixed diet, intermediate for the fine and coarse diets and the lowest for the medium diet. A similar numerical trend (p = 0.103) was observed for the apparent faecal energy digestibility coefficient. It is concluded that, with barley based diets, a variation in average particle size between $400{\mu}m$ and $1,100{\mu}m$ had no effect on pig performance but the fine dietary particle size affected the integrity of the stomach, as well as the structure of the small intestine, thus compromising overall gut health. Our data also demonstrate that changes in particle size distribution during the digestion process, rather than average particle size or particle size variation, are related to apparent faecal digestibility.

• Imaging Science in Dentistry
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• 제50권2호
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• pp.161-168
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• 2020

Purpose: The aim of this study was to evaluate the influence of voxel size and different post-processing algorithms on the analysis of dental materials using micro-computed tomography (micro-CT). Materials and Methods: Root-end cavities were prepared in extracted maxillary premolars, filled with mineral trioxide aggregate (MTA), Biodentine, and Intermediate Restorative Material (IRM), and scanned using micro-CT. The volume and porosity of materials were evaluated and compared using voxel sizes of 5, 10, and 20 ㎛, as well as different software tools(post-processing algorithms). The CTAn or MeVisLab/Materialise 3-matic software package was used to perform volume and morphological analyses, and the CTAn or MeVisLab/Amira software was used to evaluate porosity. Data were analyzed using 1-way ANOVA and the Tukey test(P<0.05). Results: Using MeVisLab/Materialise 3-matic, a consistent tendency was observed for volume to increase at larger voxel sizes. CTAn showed higher volumes for MTA and IRM at 20 ㎛. Using CTAn, porosity values decreased as voxel size increased, with statistically significant differences for all materials. MeVisLab/Amira showed a difference for MTA and IRM at 5 ㎛, and for Biodentine at 20 ㎛. Significant differences in volume and porosity were observed in all software packages for Biodentine across all voxel sizes. Conclusion: Some differences in volume and porosity were found according to voxel size, image-processing software, and the radiopacity of the material. Consistent protocols are needed for research evaluating dental materials.

• 디지털융복합연구
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• 제11권5호
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• pp.325-334
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• 2013

본 연구는 우리나라 도시 지역 성인을 대상으로 걷기운동 정도와 건강관련 삶의 질과의 관련성을 살펴보는데 목적이 있다. 제4기 3차년도(2009) 국민건강영양조사 자료에서 피조사자들 중, 신체활동 설문과 EQ-5D의 5개차원에 대해 적어도 하나 이상 답을 하지 않은 723명을 제외한, 도시에 거주하는 만 19세 이상 남자 3,892명, 여자 3,959명을 포함한 총 성인인구 7,851명을 대상으로 하였다. 연구 결과 건강관련 삶의 질은 성별, 교육수준, 배우자 유무, 월평균소득, 스트레스, 만성질환 수, 건강인식 그리고 걷기운동실천 여부가 통계적으로 유의한 관련을 보였다. 특히 걷기운동 실천을 하는 사람이 그렇지 않은 사람보다 삶의 질이 더 높게 나타났는데 이는 신체 활동을 통한 삶의 질이 비활동군에 비해서 고강도 or 중등도 보다는 걷기운동이 유의한 차이를 보였다. 이는 걷기운동을 할 경우에 건강에 따른 삶의 질이 높아지고 있다는 것을 알 수 있었다. 따라서 지역사회 주민들의 건강관련 삶의 질 수준을 높이기 위해서는 본 연구에서 확인된 내용을 토대로 이들에 필요한 정책적인 지원과 규칙적인 걷기 운동을 통한 효과적인 운동프로그램 개발이 필요할 것으로 생각된다.

• Investigative Magnetic Resonance Imaging
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• 제21권3호
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• pp.162-170
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• 2017

Purpose: To evaluate the imaging findings of desmoid tumors using various imaging modalities and to evaluate whether diffusion-weighted imaging (DWI) can help differentiate between desmoid and malignant tumors. Materials and Methods: The study included 27 patients with pathologically confirmed desmoid tumors. Two radiologists reviewed 23 computed tomography (CT), 12 magnetic resonance imaging (MRI) and 8 positron emission tomography-computed tomography (PET-CT) scans of desmoid tumors and recorded data regarding the shape, multiplicity, size, location, degree of enhancement, and presence or absence of calcification or hemorrhage. The signal intensity of masses on T1- and T2-weighted imaging and the presence or absence of whirling or band-like low signal intensity on T2-weighted imaging were recorded. The apparent diffusion coefficient (ADC) values of the desmoid tumors in nine patients with DWIs were compared with the ADC values of 32 malignant tumors. The maximum standardized uptake value ($SUV_{max}$) on PET-CT images was measured in 8 patients who underwent a PET-CT. Results: The mean size of the 27 tumors was 6.77 cm (range, 2.5-26 cm) and four tumors exhibited multiplicity. The desmoid tumors were classified by shape as either mass forming (n = 18), infiltrative (n = 4), or combined (n = 5). The location of the tumors was either intra-abdominal (n = 15), within the abdominal wall (n = 8) or extra-abdominal (n = 4). Among the 27 tumors, 21 showed moderate to marked enhancement and 22 showed homogeneous enhancement. Two tumors showed calcifications and one displayed hemorrhage. Eleven of the 12 MR T2-weighted images showed whirling or band-like low signal intensity areas in the mass. The mean ADC value of the desmoid tumors ($1493{\times}10^{-6}mm^2/s$) was significantly higher than the mean of the malignant soft tissue tumors ($873{\times}10^{-6}mm^2/s$, P < 0.001). On the PET-CT images, all tumors exhibited an intermediate $SUV_{max}$ (mean, 3.7; range, 2.3-4.5). Conclusion: Desmoids tumors showed homogenous, moderate to marked enhancement on CT and MRI scans and a characteristic whirling or band-like pattern on T2-weighted images. DWI can be useful for the differentiation of desmoid tumors from malignant soft tissue tumors.

• 한국환경생태학회지
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• 제22권5호
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• pp.505-513
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• 2008

본 연구의 목적은 인간의 간섭 및 영향에 따른 도시지역에서의 식물종의 분포패턴 및 특성을 분석하는데 있다. 전주시에서 자생적으로 이입된 모든 유관속 식물종에 대한 포괄적 자료습득을 위하여 각 1헥타 크기의 106개 표본 조사구가 분석되었다. 이러한 표본조사구는 전주시의 전형적인 토지이용유형을 대표하고 도심지역으로부터 다양한 이격거리를 갖도록 선정되었다. 종풍부성, 생활형의 패턴 그리고 외래종의 비율이 표본조사구의 헤메로비 정도와의 관계에 대해서 분석되었다. 식물종의 수는 적당히 영향을 받는 부지에서 가장 높게 나타남을 알 수 있었다. 이는 적당한 교란은 종풍부성 증가를 유도한다는 중간 교란 가설에 상응하는 결과이다. 인간의 영향을 가장 많이 받는 부지의 식물상은 일년생 식물과 외래종의 비율이 가장 높은 특징을 보였다. 그리고 일부 식물종들은 토지이용유형과 도시구역에 상응하는 유사한 분포를 갖는 세 가지 그룹인 도시지역 기피종(땅비싸리, 졸참나무, 개옻나무 등), 도시지역 및 외곽지역에 널리 분포하는 종(닭의장풀, 개망초, 박주가리 등)과 도시지역 선호종(애기땅빈대, 개쑥갓, 서양민들레 등)으로 구분되었다.

• 한국정보과학회논문지:시스템및이론
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• 제27권7호
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• pp.619-628
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• 2000

원근 투영에 의해 만들어 진 영상은 물체에 대한 거리감을 얻게 해 줌으로써 복잡한 구조물을 해석하는 데 도움을 준다. 현재 원근 투영 볼륨 렌더링의 가장 큰 단점은 실행시간이 길다는 점에 있다. 본 논문에서는 원근 광선들 간의 규칙성을 이용하여 원근 투영 볼륨 렌더링을 효율적으로 처리하는 방법을 소개한다. 볼륨의 한 면과 평행인 중간 화상의 각 수평 스캔라인과 수직 스캔라인으로부터 출발한 광선들에 대해 원형(template)들을 만든다. 광선 위의 샘플들은 원형에 미리 계산되어 있는 가중치를 사용하여 샘플 주변의 복셀들을 가중치 평균하여 얻는다. 이러한 원형의 사용은 볼륨 렌더링에서 많은 부분을 차지하고 있는 재샘플링 시간을 줄일 수 있게 해 준다. 뿐만 아니라, 본 알고리즘은 화상순서가 아니라 객체순서로 렌더링을 하므로 각 복셀을 한번 씩 만 접근하여 처리하고 런-길이 부호화된 볼륨을 사용하여 데이타 응집성의 특성을 이용함으로써 알고리즘을 더욱 가속화한다. 또한, 볼륨을 시점으로부터의 거리에 따라 여러 구역으로 나누어 각 구역에서 광선의 간격을 새로 조정함으로써 원근 광선의 발산으로 인해 샘플링 밀도가 감소하는 문제를 해결한다. 성능 평가 및 다른 방법과의 비교를 통해 원형의 사용과 객체순서 처리로 인한 속도 향상을 분석한다. 또한, 샘플링 밀도의 유지로 인해 화질이 향상된 결과를 보인다.

• 수산해양기술연구
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• 제26권2호
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• pp.151-166
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• 1990

1989년 11월 4~12일의 동지나해에서 관측한 자료를 사용하여 해황을 조사하였다. 그 결과 표층의 해황은 쓰시마 난류수와 중국 대륙 연안수로 대별되고 그 사이에는 강한 전선이 형성되고 있다. 쓰시마 난류수는 수온 22~24$^{\circ}C$, 염분 33.5~34.5$\textperthousand$의 고온.고염수로 용존산소는 4.5ml/l 이하로 낮았다. 그리고 중국 대륙 연안수는 수온 18~2$0^{\circ}C$, 염분 32.0$\textperthousand$ 이하인 저온.저염수로 용존산소는 4.5~5.0ml/l로 높게 나타났다. 저층에도 역시 표층과 같이 쓰시마 난류수와 중국 대륙 연안수가 존재하였다. 이 외에도 북쪽에는 수온 1$0^{\circ}C$ 이하, 염분 33.0$\textperthousand$ 내외, 용존 산소는 4.5ml/l 이상의 고산소수인 황해 저층냉수가 분포하고, 동남쪽에는 수온 15~19$^{\circ}C$ 염분 34.5$\textperthousand$, 용존산소는 2.0~3.5ml/l의 저산소수인 동지나해 남부 저층수도 분포하였다. 그리고 중국 대륙 연안수와 쓰시마 난류수의 중간 특성을 나타내는 수온 20.5~22.$0^{\circ}C$, 염분 32.3~33.3$\textperthousand$의 물은 중국 대륙 연안수와 쓰시마 난류가 혼합된 결과라고 판단된다.

• Applied Biological Chemistry
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• 제40권1호
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• pp.53-57
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• 1997

중성과 알카리성의 $45^{\circ}C$, 15%(v/v) dioxane 수용액중에서 살충성 imidacloprid (lUPAC:1-(6-chloro-3-pyridylmethyl)-3-N-nitro-iminoimidazolidine-2-ylideneamine)의 가수분해 반응속도 상수를 측정하고 pH-효과, 용매효과(m=0.04, n=0.30 및 m${\ll}$l), 열역학적 활성화 파라미터(${\Delta}S^{\neq}$=-0.03e.u. 및 ${\Delta}S^{\neq}=16.14\;kcal{\cdot}mol^-$), 반응 생성물 분석등의 결과로부터 반응 속도식($K_{obs}=4.56{\times}10^{-3}[OH^-]$)을 유도하여 특정 염기촉매 반응($K_{OH^-}$)으로 사면체($sp^3$) 중간체인 1-(6-chloro-3-pyridylmethyl)-2-hydroxy-2-imidazolidinylisonitraminate(I)을 거쳐 imidazolidine 고리 열림반응으로 $\beta$-3-(6-chloro-3-pyridylmethyl)aminoethyl-1-nitrourea(III)를 경유한 다음에 1-(6-chloro-3-pyridylmethyl)aminoethyl-1-nitrourea(III)으로 분해되는 일련의 친핵성 첨가-제거($Ad_N-E$) 반응메카니즘을 제안하였다. 그리고 $45^{\circ}C$의 중성(pH 8.0)에서 반감기(t1/2)는 약 4.5개월로 잔류성이 큰 화합물임을 알았다.

• Journal of Acupuncture Research
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• 제18권3호
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• pp.154-170
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• 2001

Objective : To broaden understanding about acupuncture and moxibustion therapy on AIDS and to promote base studies and clinical trials Materials and Methods : Analysis was given to more than 30 literatures including acupuncture and moxibustion therapy on AIDS-related sites explored by internet search engine named NAVER from Nov., 2000 to Feb. 20th, 2001 Results : 1. Acupuncture and moxibustion played great role as a complementary therapy in enabling AIDS patients to keep their antiretroviral therapy by enhancing immune system, ameliorating AIDS-related symptoms and side effect of antiretroviral drug 2. Acupuncture and moxibustion therapy had a broad spectrum indication from systemic or local signs of AIDS patients to signs of antiretroviral drug-related side effect 3. Contraindication of acupuncture and moxibustion therapy against AIDS patients include abstraction and moxibustion on the skin lesion, because of their easy exposure to inflammation 4. AIDS patients were regarded as the state of KI-HE(氣虛), EUM-HE(陰虛), YEOL-DOK(熱毒) in general 5. BO-KI(補氣), BO-HYUL(補血), BO-EUM(補陰), CHEONG-YEOL-HAE-DOK(淸熱解毒) were shown as a principle of acupuncture and moxibustion therapy for AIDS patients 6. Principle of selecting acupoints for AIDS patients had characteristics of enhancing immune system, detoxicating detrimental agents and relieving each AIDS related symptom appropriately 7. Acupuncture on 合谷(HAPKOK, LI4), 內關(NAE-GWAN, P6), 足三里(CHOK-SAMNI, S36) were applied to the early stage of AIDS in order to enhance immune system. Acupuncture on 血海(HYOLHAE, SP10), 三陰交(SAMUMGYO, SP6), (KOHWANG, B43) were applied to the intermediate stage of AIDS so as to enhance immune system and eliminate YEOL-DOK(熱毒) in blood. Moxibustion on 湧泉(YONGCHON, K1), 足三里(CHOK-SAMNI, S36) were applied to the late stage owing to enhance immune system more. Conclusion : The efficacy of acupuncture and moxibustion therapy on AIDS has been acknowledged to the world, moreover, it is proved to be significant as a complementary therapy on AIDS patients. Thus, more control group studies of the efficacy of acupuncture and moxibustion therapy on AIDS and clinical trials are considered to be necessary.