• Applied Biological Chemistry
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• v.47 no.1
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• pp.33-40
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• 2004

Thermodynamic properties of ubiquitin transient folding intermediate were studied by measuring folding kinetics in varying temperatures and denaturant concentrations. Through quantitative kinetic modeling, the equilibrium constant, hence folding free energy, between unfolded state and intermediate state in several different temperatures were calculated. Using these values, the thermodynamic parameters were estimated. The heat capacity change $({\Delta}C_p)$ upon formation of folding intermediate from unfolded state were estimated to be around 80% of the overall folding reaction, indicating that ubiquitin folding intermediate is highly compact. At room temperature, the changes of enthalpy and entropy upon formation of the intermediate state were observed to be positive. The positive enthalpy change suggests that the breaking up of the highly ordered solvent structure surrounding hydrophobic side-chain upon formation of intermediate state. This positive enthalpy was compensated for by the positive entropy change of whole system so that formation of transient intermediate has negative free energy.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 1994.10a
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• pp.79-84
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• 1994

The purpose of this study is to find out the relation between the outdoor activity and independent behavior of infant and the physical charater of the intermediate-territory. Another purpose is to find out the estimate of infant smother to the intermediate-territory as the space of the infant's outdoor life. As the result of this study, the following points are confirmed :1) The outdoor activity. cognition. independent behavior of infant is affected by the physical character of the intermediate-territory.2) The time and frequency of outdoor activity is affected by the physical character of the intermediate-territory. 3) The cognition and the boundary of outdoor activity are affected by the physical character of the intermediate-territory.4) The time of independent behavior is affected by the physical character of the intermediate-territory.5) The estimate of infant's mother to the intermediate-territory as the space of infant’s activity is very bad. Therefore the physical environment of intermediate-territory is very poor.

• Journal of the Korean housing association
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• v.6 no.2
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• pp.43-49
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• 1995

The purpose of this study is to find out the relation between the outdoor activity and independent behavior of infant and the physical charater of the intermediate-territory. Another purpose is to find out the estimate of infant's mother to the intermediate-territory as the space of the infant's outdoor life. As the result of this study, the following points are confirmed: 1) The outdoor activity, cognition, independent behavior of infant is affected by the physical character of the intermediate-territory. 2) The time and frequency of outdoor activity is affected bu the physical character of the intermediate-territory. 3) The cognition and the boundary of outdoor activity are affected by the physical character of the intermediate-territory. 4) The time of independent behavior is affected by the physical character of the intermediate-territory. 5) The estimate of infant's mother to the intermediate-territory as the space of infant's activity is very bad. The physical environment of intermediate-territory is very poor.

• Cross-Cultural Studies
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• v.20
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• pp.67-111
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• 2010

This paper examined the role of intermediate texts in the writing process in the context of an EFL composition class. From the tradition of teacher research, this study examined how the Korean college students in different proficiency groups created intermediate texts and used them while composing their own writing. The students produced various types of intermediate texts during the compositing process, which could serve as a basis of their writing. However, the patterns of using these intermediate texts differed widely across the proficiency groups. A writing cycle for the low proficiency group, or "surface reading-few intermediate texts-writing," indicates that less proficient students tended to engage in reading in separation of writing practices and thus produced few intermediate texts through their literacy practices. On the other hand, the students in the higher proficiency groups revealed the more integrated pattern (i.e., purpose reading/intermediate texts/writing), indicating that they often engaged in reading with specific writing purposes, practiced reading in connection to other writing practices, and elaborated written intermediate texts produced. This study argues that, to shift our student writers to a higher level category, we as teachers need to help them engage in reading and writing practices in the way they produce and use intermediate texts appropriate to their specific writing purposes.

• BMB Reports
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• v.36 no.4
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• pp.359-366
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• 2003

The effects of zinc on arginine kinase and its collapsed-state intermediate were studied. Both arginine kinase and the collapsed-state intermediate were inactivated in the presence of zinc, following a biphasic kinetic course. The corresponding apparent rate constants of inactivation at different zinc concentrations and conformational changes in the presence of 0.5 mM zinc were obtained. The conformational changes of arginine kinase and the collapsed-state intermediate were followed by fluorescence spectra and circular dichroism spectra. Comparison of the results for arginine kinase and the collapsed-state intermediate showed that the collapsed-state intermediate was more susceptible to zinc, which indicated that the collapsed-state intermediate was more flexible and unstable than arginine kinase. The special structure of arginine kinase might explain these diverse phenomena.

• Transactions of Materials Processing
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• v.19 no.4
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• pp.242-247
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• 2010

In the multi-pass shape drawing process, it is important to design the intermediate dies for producing sound products. Up to now, the design of the intermediate dies is mainly carried out by the industrial experts based on their experience. In this study, a design program was developed to design the intermediate dies for multi-pass shape drawing process. The program was programmed by using VisualLISP. In this program the intermediate dies can be designed by using the initial material shape and the final product shape. In order to verify the effectiveness, the program was applied to design the intermediate dies of multi-pass shape drawing for producing four teeth spline and gun slide. Finally, FE analysis and shape drawing experiment were performed to verify the effectiveness of the designed intermediate dies. As a result, it was possible to produce the drawn products with the required dimensional accuracy.

• Journal of the Korea Society of Computer and Information
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• v.23 no.12
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• pp.123-129
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• 2018

The purpose of this paper is to draw a conclusion on the impact of intermediate software on enterprise results. In this paper, product innovation and product reliability are especially used as analytical factors. An exploratory analytical study is conducted on the competitiveness of intermediate software, in the hope of gaining a new understanding of the competitiveness of intermediate software. Data are analyzed using such quantitative analytical tools as SPSS and AMOS. Using reliability analysis, validity analysis and structural equation model analysis, the final results are achieved. According to the analysis results, we can draw the following conclusions: the competitiveness of intermediate software has a positive impact on the innovation of software products. The competitiveness of intermediate software doesn't have a positive impact on the reliability of software products. Product innovation has a positive impact on enterprise results. Product reliability also has a positive impact on enterprise results. By analyzing the conclusions, we can make certain suggestions and draw implications on the competitiveness of China's software industry.

• Korea Trade Review
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• v.41 no.1
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• pp.159-186
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• 2016

This study analyzes the degree of dependency and comparative advantage of each country for intermediate goods trade in East Asia, which predicts the comparative advantage of the intermediate goods trade and fragmentation in East Asia when the FTA arrangement in East Asia is implemented. The results are as follows. First, the share of intra-regional trade in the intermediate goods in East Asia has increased over time, implying the deepening of interdependency in intermediate goods trade within the East Asia. Second, Korea is a net exporter in intermediate goods trade for China and ASEAN, whereas it is a net importer for Japan. Japan is a high net exporter for all East Asia, while China is a net importer for Korea, Japan and ASEAN. If FTA arrangement in East Asia is implemented, Japan and Korea will be key suppliers of the intermediate goods for East Asia, while China and ASEAN will play a role of the manufacturing factory through the import of intermediate goods. Third, Korea has a comparative advantage in intermediate trade of electric and electronics and transport vehicle industry in East Asia. Japan has a comparative advantage in all of electric and electronics, transport vehicle, precision instrument, general machinery industry, whereas China has a comparative advantage only in electric and electronics intermediate trade in East Asia. The intra-industry trade of the intermediate goods in precision instrument, general machinery industry is expected to grow among Korea, Japan and China.

• Corrosion Science and Technology
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• v.19 no.2
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• pp.51-56
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• 2020

The objective of this study was to improve properties of plasma sprayed HAp layer to titanium substrate by introducing an intermediate layer with two different methods. Before applying Zn doped HAp coating on titanium substrate, an intermediate layer was introduced by titanium plasma spray or titanium anodization. Heat treatments were conducted for some samples after titanium intermediate layer was formed. Zn doped HAp top layer was applied by plasma spraying. Three-point bending test and pull-off adhesion test were performed to determine the adhesion of Zn doped HAp coatings to substrates. Long-term credibility of Zn doped HAp plasma sprayed coatings on titanium was assessed by electrochemical impedance measurements in Hanks' solution. It was found that both titanium plasma sprayed and titanium anodized intermediate layer had excellent credibility. Strong adhesion to the titanium substrate was confirmed after 12 weeks of immersion for coating samples with titanium plasma sprayed intermediate layer. Samples with titanium anodized intermediate layer showed good bending strength. However, they showed relatively poor resistance against pulling off. The thickness of titanium anodized intermediate layer can be controlled much more precisely than that of plasma sprayed one, which is important for practical application.

• Advances in nano research
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• v.12 no.4
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• pp.365-374
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• 2022

Intermediate filaments are the mechanical ropes for both cytoskeleton and nucleoskeleton of the cell which provide tensile force to these skeletons. In providing the mechanical support to the cell, they are likely to buckle. We used conventional Euler buckling model to find the critical buckling force under different boundary conditions which they assume during different functions. However, there are many experimental and theoretical studies about other cytoskeleton components which demonstrate that due to mechanical coupling with the surrounding surface, the critical buckling force increases considerably. Motivated with these results, we also investigated the influence of surface effects on the critical buckling force of intermediate filaments. The surface effects become profound because of increasing ratio of surface area of intermediate filaments to bulk at nano-scale. The model has been solved analytically to obtain relations for the critical forces for the buckling of intermediate filaments without and with surface effects. We found that critical buckling force with surface effects increases to a large extent due to mechanical coupling of intermediate filaments with the surrounding surface. Our study may be useful to develop a unified experimental protocol to characterize the physical properties of Intermediate filaments and may be helpful in understanding many biological phenomenon involving intermediate filaments.