• Title/Summary/Keyword: Intermedia

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Some Observations on the Fine Structure of Korean Bat Livers (한국산 박쥐 간장의 미세구조적 고찰)

  • Choi, Byung-Jin;Uhm, Chang-Sub;Chang, Byung-Jun;Park, Chang-Hyun
    • Applied Microscopy
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    • v.31 no.4
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    • pp.375-383
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    • 2001
  • To understand the fine structure of Korean bat livers , the authors investigated the liver of four Korean bats; Rhinolephus ferrumequinum korai, Myotis macrodactylus, Myotis dauhentonii ussrinesis, and Murina leucogaster intermedia by transmission electron microscopy. The hepatocytes of Rhinolophus ferrumequinum korai had large-sized mitochondria and many peroxisomes. In the Myotis macrodactylus, juctional completes, especially desmosomes, were well developed. The Myotis daubentonii ussrinesis had many glycogen particles in the cytoplasm. Also, the space of Disse and sinusoidal spare was filled with amorphous materials. In the Murina leucogaster intemedia, basement membrane was prominent in the sinusoid, and no Kupffer and Ito cells were observed These results suggest the characteristic differences in the liver ultrastructure among Korean bats.

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Chigger Mite (Acari: Trombiculidae) Survey of Rodents in Shandong Province, Northern China

  • Huang, Xiao-Dan;Cheng, Peng;Zhao, Yu-Qiang;Li, Wen-Juan;Zhao, Jiu-Xu;Liu, Hong-Mei;Kou, Jing-Xuan;Gong, Mao-Qing
    • Parasites, Hosts and Diseases
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    • v.55 no.5
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    • pp.555-559
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    • 2017
  • Chigger mites are parasites of rodents and other vertebrates, invertebrates, and other arthropods, and are the only vectors of scrub typhus, in addition to other zoonoses. Therefore, investigating their distribution, diversity, and seasonal abundance is important for public health. Rodent surveillance was conducted at 6 districts in Shandong Province, northern China ($114-112^{\circ}E$, $34-38^{\circ}N$), from January to December 2011. Overall, 225/286 (78.7%) rodents captured were infested with chigger mites. A total of 451 chigger mites were identified as belonging to 5 most commonly collected species and 3 genera in 1 family. Leptotrombidium scutellare and Leptotrombidium intermedia were the most commonly collected chigger mites. L. scutellare (66.2%, 36.7%, and 49.0%) was the most frequently collected chigger mite from Apodemus agrarius, Rattus norvegicus, and Microtus fortis, respectively, whereas L. intermedia (61.5% and 63.2%) was the most frequently collected chigger mite from Cricetulus triton and Mus musculus, respectively. This study demonstrated a relatively high prevalence of chigger mites that varied seasonally in Shandong Province, China.

DETECTION OF BLACK-PIGMENTED BACTERIA IN INFECTED ROOT CANALS (감염근관에서 Black-Pigmented Bacteria의 동정에 대한 연구)

  • Kwon, Eun-Kyoung;Kim, Eun-Sook;Kwak, Ju-Seog;Lee, Hwang;Lee, Su-Jong;Im, Mi-Kyung
    • Restorative Dentistry and Endodontics
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    • v.27 no.1
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    • pp.54-65
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    • 2002
  • Black-pigmented bacteria have been implicated in the endodontic infections. This group of microorganisms includes Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens. The organisms display a wide variety of virulence factors that may be pertinent to acute endodontic infections. The aim of this study was to identify P. endodontalis, P. gingivalis, P. intermedia, and P. nigrescens by using special potency disk test, filter paper spot test, 165 rRNA gene-directed PCR, and API 32A. Microbial samples were collected from root canals of 33 intact teeth with necrotic pulp and/or apical periodontitis. Conventional laboratory methods were used for identification of the strains of black pigmented bacteria. Eighteen of 33 samples were positive for the growth of black-pigmented bacteria Five colonies were cultured from each pure cultured colonies from Brucella agar plate. Seventy seven colonies were positive for the growth of black-pigmented bacteria. Thirty three of 77(42.6%) were identifed as P. nigrescens, 10 of 77(12.9%)were P. gingivalis, 6 of 77(7.8%) were P. endodontalis, 10 of 77(12.9%) were P. intermedia. On the contrary the reference strains of P. nigrescens, experimental strains of P nigrescens was sensitive to kanamycin in special potency disk test. 165 rRNA gene PCR and API test after rapid presumptative identification methods, such as special potency disk test and filter paper spot test, would be accurate detection methods for black-pigemented bacteria.

Identification of the Black-pigmented Prevotella Species and Porphyromonas Species from Infected Dental Root Canals (치아 근관 감염 검체에서 검은 색소 형성 Prevotella species와 Porphyromonas species의 동정)

  • Kim, Eun-Sook;Kim, Shin-Moo
    • Korean Journal of Clinical Laboratory Science
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    • v.38 no.1
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    • pp.45-53
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    • 2006
  • Anaerobic black-pigmented bacteria have been implicated in the endodontic infections. This group of microorganisms includes Porphyromonas endodontalis, P. gingivalis, Prevotella intermedia, and Prevotella nigrescens. The organisms display a wide variety of virulence factors that may be pertinent to acute endodontic infections. The aim of this study was to identify P. endodontalis, P. gingivalis, P. intermedia, and P. nigrescens by using the special potency disk test, filter paper spot test, 16S rRNA gene-directed PCR, and API 32A system. Microbial samples were collected from root canals of 33 intact teeth with necrotic pulp and apical periodontitis. Conventional laboratory methods were used to identify the strains of anaerobic black pigmented bacteria. Eighteen out of 33 samples were positive for the growth of black-pigmented bacrteria. Five colonies were cultured from each pure cultured colony from Brucella agar plates. Seventy seven colonies were positive for the growth of black-pigmented bacteria. Thirty three out of 77(42.8%) were identifed as P. nigrescens, 10 out of 77(13%)were P. gingivalis, 6 out of 77(7.8%) were P. endodontalis, 10 out of 77(13%) were P. intermedia. On the contrary the reference strains of P. nigrescens, experimental strains of P. nigrescens were susceptible to kanamycin in the special potency disk test. We concluded that after rapid presumptive identification methods, such as the special potency disk test and filter paper spot test were done, 16S rRNA gene PCR and API 32A test would be accurate detection methods for black-pigemented bacteria.

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Relationship between liver iron concentration determined by R2-MRI, serum ferritin, and liver enzymes in patients with thalassemia intermedia

  • Al-Momen, Hayder;Jasim, Shaymaa Kadhim;Hassan, Qays Ahmed;Ali, Hayder Hussein
    • BLOOD RESEARCH
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    • v.53 no.4
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    • pp.314-319
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    • 2018
  • Background Iron overload is a risk factor affecting all patients with thalassemia intermedia (TI). We aimed to determine whether there is a relationship of serum ferritin (SF) and alanine aminotransferase (ALT) with liver iron concentration (LIC) determined by R2 magnetic resonance imaging (R2-MRI), to estimate the most relevant degree of iron overload and best time to chelate in patients with TI. Methods In this cross-sectional study, 119 patients with TI (mean age years) were randomly selected and compared with 120 patients who had a diagnosis of thalassemia major (TM). Correlations of LIC, as determined by R2-MRI, with SF and ALT levels, were assessed in all participants. A P-value <0.05 was considered statistically significant. Results SF and LIC levels were lower in patients with TI than in those with TM; only ferritin values were significant. We found a statistically significant relationship between SF and LIC, with cut-off estimates of SF in patients with TI who had splenectomy and those who entered puberty spontaneously (916 and 940 ng/mL, respectively) with LIC >5 mg Fe/g dry weight (P<0.0001). A significant relationship was also found for patients with TI who had elevated ALT level (63.5 U/L), of 3.15 times the upper normal laboratory limit, using a cut-off for LIC ${\geq}5mg\;Fe/g\;dry\;weight$. Conclusion We determined the cut-off values for ALT and SF indicating the best time to start iron chelation therapy in patients with TI, and found significant correlations among iron overload, SF, and ALT.