• 한국정보통신학회논문지
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• 제18권1호
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• pp.32-41
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• 2014

현재 이동통신3사가 보유한 총 주파수는 390MHz에 이르지만 LTE 데이터 트래픽의 경우 1년 기준으로 약 6배가량 증가하고 있는 상황에서 폭증하는 트래픽을 감당하기 위해서는 좀 더 많은 주파수 확보가 필요하다. 2.1GHz 대역은 주파수 특성, 대역의 연속성 측면에서 이동통신용 주파수로 이용할 가치가 있다. 본 논문에서는 2.1GHz 대역을 LTE-Advanced로 활용하기 위해 인접 지역과의 양립을 위한 국제법규를 분석하였다. 또한, 간섭평가를 실시하고 그 결과 값 도출을 통해 인접 대역과 양립 가능함을 증명하였다.

• 한국전자파학회논문지
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• 제28권3호
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• pp.177-185
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• 2017

본 논문에서는 비면허 대역 무선기기에 사용되는 주파수 공동사용 기술의 성능을 비교 분석할 수 있는 간섭부하 개념을 제안하였다. 간섭부하 개념은 다양한 주파수 공동사용 기술, 즉 주파수 호핑(FH: Frequency Hopping), 듀티 사이클(DC: Duty Cycle), 송신전 감지 기술을 사용하는 무선기기가 타 무선기기에 간섭을 주는 정도를 정량적으로 비교할 수 있다. 따라서 주파수 공동사용 기술기준이 일관되고 합리적으로 제정되었는지 평가하는데 도움을 줄 수 있다. 제안한 개념을 검증하기 위하여 다양한 2.4 GHz 대역의 무선기기의 주파수 공동사용 성능을 비교분석하였다. 또한, 실시간 테스트베드를 구축하고, FH과 DC의 경우, 기술기준에 따른 비트 오류 확률을 비교실험하였다. 실험 결과, 10개 채널을 FH하는 것과 10 % DC이 동일한 오류 확률을 가짐을 확인함으로써 본 논문에서 제안한 개념의 타당성을 확인하였다.

• Clinical and Experimental Reproductive Medicine
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• 제39권2호
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• pp.58-67
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• 2012

Objective: Previously, we identified that transketolase (Tkt), an important enzyme in the pentose phosphate pathway, is highly expressed at 2 hours of spontaneous maturation in oocytes. Therefore, this study was performed to determine the function of Tkt in meiotic cell cycle regulation, especially at the point of germinal vesicle breakdown (GVBD). Methods: We evaluated the loss-of-function of Tkt by microinjecting Tkt double-stranded RNAs (dsRNAs) into germinal vesicle-stage oocytes, and the oocytes were cultured in vitro to evaluate phenotypic changes during oocyte maturation. In addition to maturation rates, meiotic spindle and chromosome rearrangements, and changes in expression of other enzymes in the pentose phosphate pathway were determined after Tkt RNA interference (RNAi). Results: Despite the complete and specific knockdown of Tkt expression, GVBD occurred and meiosis was arrested at the metaphase I (MI) stage. The arrested oocytes exhibited spindle loss, chromosomal aggregation, and declined maturation promoting factor and mitogen-activated protein kinase activities. The modified expression of two enzymes in the pentose phosphate pathway, Prps1 and Rbks, after Tkt RNAi and decreased maturation rates were amended when ribose-5-phosphate was supplemented in the culture medium, suggesting that the Tkt and pentose phosphate pathway are important for the maturation process. Conclusion: We concluded that Tkt and its associated pentose phosphate pathway play an important role in the MI-MII transition of the oocytes' meiotic cell cycle, but not in the process of GVBD.

• Asian Pacific Journal of Cancer Prevention
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• 제13권3호
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• pp.753-759
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• 2012

Radixin, encoded by a gene on chromosome 11, plays important roles in cell motility, invasion and tumor progression. However, its function in pancreatic cancer remains elusive. In this study, radixin gene expression was suppressed with a lentivirus-mediated short-hairpin RNA (shRNA) method. We found that radixin shRNA caused down-regulation of radixin in PANC-1 cells, associated with inhibition of pancreatic cancer cell proliferation, survival, adhesion and invasive potential in vitro. When radixin-silenced cells were implanted in nude mice, tumor growth and microvessel density were significantly inhibited as compared to blank control cells or nonsense shRNA control cells. Thrombospondin-1 (TSP-1) and E-cadherin were up-regulated in radixin-silenced PANC-1 cells. Our results suggest that radixin might play a critical role in pancreatic cancer progression, possibly through invvolvement of down-regulation of TSP-1 and E-cadherin expression.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.629-635
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• 2014

Background: Acute myeloid leukemia (AML) is a fatal hematological malignancy which is resistant to a variety of chemotherapy drugs. Myeloid cell leukemia-1 (Mcl-1), a death-inhibiting protein that regulates apoptosis, has been shown to be overexpressed in numerous malignancies. In addition, it has been demonstrated that the expression level of the Mcl-1 gene increases at the time of leukemic relapse following chemotherapy. The aim of this study was to target Mcl-1 by small interference RNA (siRNA) and analyze its effects on survival and chemosensitivity of acute myeloid leukemia cell line HL-60. Materials and Methods: siRNA transfection was performed with a liposome approach. The expression levels of mRNA and protein were measured by real-time quantitative PCR and Western blot analysis, respectively. Trypan blue assays were performed to evaluate tumor cell growth after siRNA transfection. The cytotoxic effects of Mcl-1 siRNA (siMcl-1) and etoposide were determined using MTT assay on their own and in combination. Apoptosis was quantified using a DNA-histone ELISA assay. Results: Transfection with siMcl-1 significantly suppressed the expression of Mcl-1 mRNA and protein in a time-dependent manner, resulting in strong growth inhibition and spontaneous apoptosis. Surprisingly, pretreatment with siMcl-1 synergistically enhanced the cytotoxic effect of etoposide. Furthermore, Mcl-1 down-regulation significantly increased apoptosis sensitivity to etoposide. No significant biological effects were observed with negative control siRNA treatment. Conclusions: Our results suggest that specific suppression of Mcl-1 by siRNA can effectively induce apoptosis and overcome chemoresistance of leukemic cells. Therefore, siMcl-1 may be a potent adjuvant in leukemia chemotherapy.

• 한국환경복원기술학회지
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• 제11권2호
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• pp.104-113
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• 2008

Due to the increasing needs of land supply, green space has been continuously encroached and resulted considerable naturality deterioration in Korea. In order to overcome the problem, it is necessary to introduce a integrated system for managing the quantitative and qualitative aspects of green space. With this regard, this study is to derive the feasibility for the adoption of the 'no net loss of green' policy to Korea from German and Japanese experiences. In Germany, natural resources are protected and inevitable damages were compensated through the adoption of 'interference regulation'. In Japanese case, future green space and artificial green foundation should be specified on the green basic plan which pursuit the improvement of naturality. In order to introduce the 'no net loss of green' policy to Korea, not only awareness raising on integrated green management system but also amendment of related regulations are needed. In detail, restoration and recovery on the damaged nature should be clearly elucidated and indicators to evaluate the current naturality and calculation methods for the restoration should be developed. Should this integrated green management system introduced, deterioration of naturality by development activities could be minimized thorough the adoption of land development permission criteria and green space management methodologies.

• Journal of Microbiology and Biotechnology
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• 제19권8호
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• pp.765-773
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• 2009

Edwardsiella tarda is a pathogen with a broad host range that includes human and animals. The E. tarda hemolysin (Eth) system, which comprises EthA and EthB, is a noted virulence element that is widely distributed in pathogenic isolates of E. tarda. Previous study has shown that the expression of ethB is regulated by iron, which suggests the possibility that the ferric uptake regulator (Fur) is involved in the regulation of ethB. The work presented in this report supports the previous findings and demonstrates that ethB expression was decreased under conditions when the E. tarda Fur ($Fur_{Et}$) was overproduced, and enhanced when $Fur_{Et}$ was inactivated. We also identified a second ethB regulator, EthR, which is a transcription regulator of the GntR family. EthR represses ethB expression by direct interaction with the ethB promoter region. In addition to ethB, EthR also modulates, but positively, luxS expression and AI-2 production by binding to the luxS promoter region. The expression of ethR itself is subject to negative autoregulation; interference with this regulation by overexpressing ethR during the process of infection caused (i) drastic changes in ethB and luxS expressions, (ii) vitiation in the tissue dissemination and survival ability of the bacterium, and (iii) significant attenuation of the overall bacterial virulence. These results not only provide new insights into the regulation mechanisms of the Eth hemolysin and LuxS/AI-2 quorum sensing systems but also highlight the importance of these systems in bacterial virulence.

• Molecular & Cellular Toxicology
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• 제5권2호
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• pp.133-140
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• 2009

Caveolin may be a molecular target for modulation of aging process in cochlear hair cells and have association with oxotoxicity. First we investigated the basal expression of caveolin-1, caveolin-2, caveolin-3, nitric oxide synthase, and superoxide dismutase in UB/OC-1 cochlear hair cell line. By using a RNA interference technique, we investigated whether down-regulation of caveolin influenced telomerase activity and reactive oxygen species (ROS) production in cochlear hair cells. In addition, cisplatin and gentamycin, known ototoxic drugs, were administered to the cochlear cells to determine their impact on caveolin expression. Further attempts at elucidating cellular aging mechanism with caveolin and ototoxic drugs were carried out. The main discoveries were the presence of caveolin-1 in UB/OC-1 cells and that down-regulation of caveolin-1 reduced protein kinase A activity. Telomerase was activated by caveolin down-regulation and caveolin down-regulation inhibited oxidative stress at the mitochondrial level. When cisplatin and gentamycin were administered to the cochlear hair cells during a caveolin expression state, a decrease in telomerase activity and increase ROS activity was observed. Caveolin-1 may modulate the senescent mechanisms in cochlear cells. An increase in caveolin-1 levels can lead to ROS production in the mitochondria which may cause ototoxicity.

• 한국전자파학회논문지
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• 제23권7호
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• pp.805-814
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• 2012

무선기기의 간헐적인 신호 또는 높고 낮은 진폭의 주기적인 신호 전송은 오디오 주파수에 간섭 신호를 발생시켜 근접 거리에 설치된 음향 기기에 잡음을 발생시킨다. 최근 이와 관련하여 Global system for Mobile Communication(GSM) 폰과 유사하게 간헐적인 신호 전송을 하는 Long Term Evolution(LTE) 폰에 의한 오디오 잡음현상이 보고되었다. 현재 국내 KN20 규격에서는 잡음에 대한 내성 능력을 확보하기 위하여 음향 및 TV 방송수신기의 내성 시험 기준 신호로 변조 주파수 1 kHz Amplitude Modulation(AM) 80 %와 Code Division Multiple Access(CDMA) 신호를 이용하고 있다. 본 논문에서는 LTE 폰의 출시에 따라 기존의 내성 기준 신호 AM과 CDMA를 GSM과 LTE 폰이 발생시키는 오디오 잡음의 수준과 비교한 후, 국내 음향 기기에 대한 내성 기준 신호의 개선을 제안한다. CDMA 신호는 음향 기기에 간섭을 거의 일으키지 않는 것으로 여겨지므로 시험 항목에서 삭제해도 무방할 것으로 판단된다. 반면, LTE는 많은 사용자가 이용하고 있으며, 음향 기기 등에 무시할 수 없는 간섭을 발생시킬 것으로 분석되므로 CDMA 신호를 대신하여 음향 기기 및 TV 방송 수신기의 내성 시험을 위한 시험 기준 신호로 규정하는 것이 바람직할 것으로 사료된다.

• 전자공학회논문지
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• 제49권12호
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• pp.277-284
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• 2012

본 논문은 EMI(Electro Magnetic Interference) 잡음의 주된 원인인 각 회로의 임피던스 부정합, 고속 데이터 인터페이스 그리고 PCB(Printed Circuit Board) 상에서 전원/접지 구조에 의한 불안정 잡음을 주파수 대역별로 분석하여 각 원인별 잡음 개선 방안을 제시하였다. 또한 이를 통해 무선 비디오 데이터를 전송하는데 사용되는 차량용 무선 비디오 스트림 시스템 설계방안을 제안하였다. 잡음 개선 방안으로 회로 신호의 임피던스 정합과 PCB 라인의 내층 설계, 전원/접지 구조 개선을 통해 시스템의 잡음 개선을 수행하였다. 구현된 시스템은 EMI 규제에 따라 각 대역에서 40[dBuV/m]와 47[dBuV/m]내로 잡음 개선을 하였다.