• 한국약용작물학회지
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• 제27권5호
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• pp.307-314
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• 2019

Background: We measured the weight characteristics, intercellular space volume as a proportion of root volume, and quantities of the functional component in Angelica gigas Nakai, collected from four cultivation areas in Pyeongchang, including Tapdong-ri and SangjinBu-ri, and tested the results for correlations. Methods and Results: Dry weight was the highest in the Tapdong-ri group ($13.37{\pm}0.13g$) and the lowest in the SangjinBu-ri group ($11.90{\pm}0.57g$). The percentage of intercellular spaces within the roots, determined by micro-CT camera, was the highest in the Tapdong-ri group ($6.44{\pm}0.76%$) and the lowest in the SangjinBu-ri group ($9.01{\pm}2.15%$). The quantity of functional components were the highest in the Tapdong-ri group ($116.13{\pm}9.53mg/g$) and the lowest in the SangjinBu-ri group ($99.77{\pm}23.23mg/g$). Conclusions: The dry weight of A. gigas significantly correlated with the volume and percentage of intercellular space. An increase in the dry weight resulted in a negative correlation between the volume and ratio of the intercellular spaces. The volume of intercellular space correlated negatively with levels of the functional components, this was significant in the case of the nodakenin, decursin and decursinol angelate content (p < 0.016).

• Journal of Plant Biology
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• 제26권1호
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• pp.41-51
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• 1983

This study was carried out to investigate ontogeny of stomata and aerenchyma tissue in Trapa natans L. var. bispinosa Makino, an aquatic plant. Ontogeny of stomata in this plant was an aperigenous type surrounding with 5 to 8 epidermal cells without subsidiary cells. Stomata were distributed abundantly on the upper surface of leaf, however, no stoma was found on the lower surface of leaf, and on the epidermis of reproductive organ, petiole and stem. Ontogency of aerenchyma tissue was progressed with five steps; 1) formation of angular cells by division of cortex cells, 2) development of small and large globular cells in accompany with schizogenous intercellular space, 3) enlargement of globular cells and more expansion of intercellular space, 4) cell induction of long elliptic and triarmed shape, 5) completion of the largest intercellular space from endodermis toepidermis. During the growth period two types of leaf were appeared at each node of stems; one type was a submerged and early-fallen leaf, the other was a floating leaf on water surface.

• Applied Microscopy
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• 제24권4호
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• pp.52-60
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• 1994

The developing ovarian oocyte of Dendrolimus spectabilis has been studied by using electron microscopical techniques. After yolk formation the vitelline membrane was laid down in the intercellular space between the follicle cell and the oocyte. But before the vitelline membrane formation the granules with high electron density that the vitelline membrane precusor are observed in the follicle cell. At the late vitellogenesis stage these granules were transported into the intercellular space between the follicle cells and the oocyte. These granules fuse to each other and larger bodies which eventually produce the vitelline membrane. The vitelline membrane was distinguished into the light inner and dark outer membrane. Next the chorion was laid down. It was apparent that the chorion was laid down in the intercellular space immediately adjacent to the vitelline membrane, and that it was formed by the follicle cells only.

• 한국가구학회지
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• 제18권3호
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• pp.243-247
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• 2007

An experiment was conducted to observe the safranine penetration depth in radial directions of Populus tomentiglandulosa. Radial penetration was considered from bark to pith. In radial direction, ray parenchyma and intercellular space were considered for the measurement of safranine penetration depth. It was found that sapwood conducted safranine 24.23% higher in radial direction compared with heartwood. Intercellular space conducted safranine 39.27% higher depth compared with ray parenchyma and the penetration depth was 39.41% higher in sapwood compared to heartwood. During safranine penetration, it formed a curvature in the lumen of ray parenchyma. Initially safranine penetration was found high and decreased gradually.

• 한국전자현미경학회:학술대회논문집
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• 한국현미경학회 1997년도 제28차 춘계학술대회
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• pp.13-13
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• 1997

• 식물조직배양학회지
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• 제22권1호
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• pp.25-25
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• 1995

• Maxillofacial Plastic and Reconstructive Surgery
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• 제11권1호
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• pp.171-186
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• 1989

This study was undertaken to investigate the effects of indomethacin on 4-nitroquinoline 1-oxide (4NQO) induced palatal carcinoma of albino rats. Sixty albino rats about 100 gms of body weight, 6 weeks old-were used classifying as 1) six albino rats of normal group received no treatment, 2) six albino rats of control group treated with propane 1, 2-diol 3 times a week, 3) twenty four albino rats of experimental group I treated with 0.5% 4NQO in propane 1, 2-diol 3 times a week, 4) twenty four albino rats of experimental group II treated with 0.5% of 4NQO in propane 1, 2-diol 3 times a week and administrated 20${\mu}g/ml$ indomethacin in drinking water ad lib. The animals of normal and control groups were sacrificed 7th, 11th, 15th, 19th, 23rd and 27th week, while those of experimental group I and II were sacrificed 7th, 9th, 11th, 13th, 15th, 17th, 19th, 21st, 23rd, 25th, 27th and 29th week after the experiment. The palatal mucosa was excised and examined grossly, light-microscopically and electron-microscopically. Following results were obtained. 1. In control group, there was no specific difference from normal tissue histopathologically. 2. In group I, hyperkeratosis mild acantosis and dyskeratosis in in 7th week, dysplasia in 11th week and severe acantosis in 19th week were observed, but squamous cell carcinoma not observed until 29th week on light-microscope. 3. In group II, hyperkeratosis, mild acantosis in 9th week, dyskeratosis and dysplasia in 21st week, severe acantosis in 27th week and squamous cell carcinoma in 29th week were observed on light-microscope. 4. In group I, widening of intercellular space in 7th week, increasing of desmosome, giant desmosome and tonofilament in cytoplasm in 9th week, severe widening of intercellular space, increasing of mitochondria and vascular degeneration in 11th week, irregular pattern of cell feature and nucleus and prominent nucleoli in 19th week, and continuity of basal lamina in 29th week were observed on electron-microscope. 5. In group II, mild widening of intercellular space in 9th week, increasing of mitochondria, vascular degeneration and tonofilament in cytoplasm in 13th week, increasing of desmosome and giant desmosome in 15th week, irregular pattern of cell surface and nucleus and prominent nucleoli, and in 21st week continuity of basal lamina were observed on electron-microscope which phenomenon occurred little later than group I. After 21st week, however, severe widening of intercellular space, vascular degeneration and continuity of basal lamina were observed as in group I.

• Applied Microscopy
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• 제22권1호
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• pp.1-14
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• 1992

Parenchyma of the cat pineal body consisted of pinealocytes and glial cells. The pinealocyte, predominant cell type, was characterized by having large mitochondria with pale matrix, abundant polyribosomes, moderately-developed Golgi apparatus, centrioles and occasional cilia. The pinealocyte had one thick and long cytoplasmic process at the one pole of the cell, and slender and shorter processes at the other pole, and in addition occasional short processes from the cell body. These processes contained longitudinally arranged microtubules, and a few mitochondria. Thick processes teminated as bulgings either in the intercellular process-rich area, or in the perivascular border which was formed by glial cell processes. These endings of pinealocyte processes had many small vesicles, mitochondria, and occasional dense bodies. Glial cells with abundant filaments of intermediate type and clear cytoplasmic matrix were fibrous astrocyte. Perikarya of the astrocytes had small and dense mitochondria, moderately developed Golgi apparatus, dense bodies and variable amount of intermediate filaments. Glial cell processes run through the intercellular spaces among the pinealocyte processes. Glial cell of protoplasmic type had no or a few filaments, but it had well-organized rough endoplasmic reticulum, dense mitochondria, well developed Golgi apparatus and many dense granules. Intercellular canaliculi formed by adjacent pinealocytes and glial cell processes were often noted. Within the parenchyma, sympathetic and parasympathetic axons and their endings were noted. These endings were present mostly in the intercellular spaces without having membrane specialization, however, in rare instances, ending with small clear and dense cored vesicles, and large dense cored vesicles formed specialized synapse with a pinealocyte process. Within the perivascular spaces nerve fibers and endings, Schwann cells and pericyte were noted. In rare case pinealocyte process penetrated into the perivascular space through the interuptions of glial border. These results suggest that pinealocyte of the cat has less significance in secretory function and is rather neural type of cell.

• 대한수의학회지
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• 제35권3호
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• pp.553-562
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• 1995

To elucidate pathologic change of skin in porcine exudative epidermitis, immunohistochemical and electron microscopical observations were carried out in the skin of the suckling pigs inoculated with Staphylococcus hyicus subsp hyicus which were isolated from natural case. In immunohistochemistry, ATPase-positive dendritic cells were more populated in epidermo-dermal junctional areas and perivascular area in dermis than in epidermal area as the disease was proceeded. These dendritic cells were identified as Langerhans cell by immunoperoxidase staining and these cells were populated granulomatous bodies. Electron microscopical study showed various retrogressive degeneration and vacuolation of epidermal cell organelles with retention of amorphorous exudates in intercellular space, and cellular seperation. Langerhans cells present in intercellular space of epidermis were populated in epidermo-dermal junctional areas, in dermis, and around granulomatous bodies. Langerhans cells contained decreased Birbeck granules in number but increased lysosome and ribosome. These cells were in contact with lymphocytes. This study was discussed relation between the various immunocytes and the formation of granulomatous bodies, and this inflammation was considered as delayed type hypersensitivity.

• Applied Microscopy
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• 제25권4호
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• pp.104-114
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• 1995

The epidermis of scales in the abdominal parts from freshwater- and seawater-adapted guppies(Poecilia reticulatus) were studied respectively by scanning- and transmission- electronmicroscope. In the seawater adapted group, the surface area of pavement cells is increased nearly twice as much as that of freshwater-adapted group, and the pavement cells are relatively flattened. Adaptatoin to seawater causes the increment of the density of glycocalyx in the surface of the microridge of pavement cells, as well as the well developed intercellular junctional complex(desmosome) between neighbouring filament-containing cells. Also, intercellular space between adjacent filament-containing cells is more frequently observed in the epidermis of seawater adapted guppy.