• 한국작물학회지
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• 제42권5호
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• pp.604-608
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• 1997

수원 작물시험장 등 전국 각도 9개 지역에서 1990 및 1991년에 걸쳐 수행된 콩 지방적응연락시험에서, 팔달콩 등 10개의 공시계통들의 평균 수량을 조사하였으며, 수량안정성을 여러가지 방법으로 분석하여 상호 비교하였다. 수량 안정성분석은 Francis와 Kannenberg의 coefficient of variability, Finlay와 Wilkinson의 regression coefficient, Eberhart와 Russell의 deviation parameter, Plaisted의 variance component, Wricke의 ecovalence등 5개 분석방법을 이용하였으며 그 결과를 요약하면 다음과 같다. 1. 분산성분 비교에 의하면, 수량에 대한 콩유전자와 지역간 상호작용 분산성분이 콩유전자 및 년차간 상호작용 분산성분보다 약 7배 높아서, 정확한 수량안정도 평가를 위해서는 여러 해 걸쳐서 콩계통을 평가하기보다는 더 많은 지역에서 평가되어야 할 것으로 보였다. 2. 2년차 9개지역에서 수원 14005('92년 태광콩으로 장려품종 지정)가 평균수량이 높았으며, 5개 안정성 분석결과 수량안정도도 높았다. 3. 안정도계수 종류들 간의 rank correlation 분석결과, Eberhart와 Russell, Plaisted, 그리고 Wricke의 방법들 간의 고도의 유의적인 상관관계가 있었으나, Francis와 Finlay 방법은 다른 분석방법들과는 상관관계가 인정되지 않았다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권9호
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• pp.1247-1253
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• 2003

Selection for increased milk production in dairy cows has often resulted in a higher incidence of disease and thus incurred a greater health costs. Considerable interests have been shown in breeding dairy cattle for disease resistance in recent years. This paper discusses the limitations of breeding dairy cattle for genetic resistance in six parts: 1) complexity of disease resistance, 2) difficulty in estimating genetic parameters for planning breeding programs against disease, 3) undesirable relationship between production traits and disease, 4) disease as affected by recessive genes, 5) new mutation of the pathogens, and 6) variable environmental factors. The hidden problems of estimating genetic and phenotypic parameters involving disease incidence were examined in terms of categorical nature, non-independence, heterogeneity of error variance, non-randomness, and automatic relationship between disease and production traits. In light of these limitations, the prospect for increasing genetic resistance by conventional breeding methods would not be so bright as we like. Since the phenomenon of disease is the result of a joint interaction among host genotype, pathogen genotype and environment, it becomes essential to adopt an integrated approach of increasing genetic resistance of the host animals, manipulating the pathogen genotypes, developing effective vaccines and drugs, and improving the environmental conditions. The advances in DNA-based technology show considerable promise in directly manipulating host and pathogen genomes for genetic resistance and producing vaccines and drugs for prevention and medication to promote the wellbeing of the animals.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6673-6680
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• 2013

Objective: Insulin resistance (IR) is an established risk factor for colorectal cancer (CRC). Given that CRC and IR physiologically overlap and the calpain-10 gene (CAPN10) is a candidate for IR, we explored the association between CAPN10 and CRC risk. Methods: Blood samples of 400 case-control pairs were genotyped, and the lifestyle and dietary habits of these pairs were recorded and collected. Unconditional logistic regression (LR) was used to assess the effects of CAPN10 SNP43 and SNP19, and environmental factors. Both generalized multifactor dimensionality reduction (GMDR) and the classification and regression tree (CART) were used to test gene-environment interactions for CRC risk. Results: The GA+AA genotype of SNP43 and the Del/Ins+Ins/Ins genotype of SNP19 were marginally related to CRC risk (GA+AA: OR = 1.35, 95% CI = 0.92-1.99; Del/Ins+Ins/Ins: OR = 1.31, 95% CI = 0.84-2.04). Notably, a high-order interaction was consistently identified by GMDR and CART analyses. In GMDR, the four-factor interaction model of SNP43, SNP19, red meat consumption, and smoked meat consumption was the best model, with a maximum cross-validation consistency of 10/10 and testing balance accuracy of 0.61 (P < 0.01). In LR, subjects with high red and smoked meat consumption and two risk genotypes had a 6.17-fold CRC risk (95% CI = 2.44-15.6) relative to that of subjects with low red and smoked meat consumption and null risk genotypes. In CART, individuals with high smoked and red meat consumption, SNP19 Del/Ins+Ins/Ins, and SNP43 GA+AA had higher CRC risk (OR = 4.56, 95%CI = 1.94-10.75) than those with low smoked and red meat consumption. Conclusions: Though the single loci of CAPN10 SNP43 and SNP19 are not enough to significantly increase the CRC susceptibility, the combination of SNP43, SNP19, red meat consumption, and smoked meat consumption is associated with elevated risk.

• 응용통계연구
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• 제24권5호
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• pp.861-869
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• 2011

최근 유전학에서 주요 목표중 하나는 복합질환에 영향을 미치는 유전적 요인을 찾아내는 것이다. 유전자좌간의 상호작용이 있을 때에는 단일 유전자좌 분석으로는 이러한 목표를 달성하기 어려우므로, 유전자-유전자간 상호작용이나 유전자-환경인자간 상호작용분석을 고려할 필요가 있다. 자주 사용되는 MDR(multifactor dimensionality reduction)방법은 데이터를 고위험군과 저위험군으로 각각 병합하여 사용하므로 특정 유전자형에서 차이가 나는 경우에는 이를 찾아내기 어렵다. 본 연구에서는 이러한 점을 보완하도록 유전자형 조합에서의 대조군과 질환군의 상대위험도를 이용하여 유전자-유전자간 상호작용을 탐색하는 방법을 제안하였다. MDR 공개데이터와 8가지 유전모형으로부터 생성한 모의자료의 분석을 통해 방법의 유용성을 확인하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.6953-6961
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• 2015

Background: Tobacco and alcohol contain or may generate carcinogenic compounds related to cancers. CYP1A1 enzymes act upon these carcinogens before elimination from the body. The aim of this study was to investigate whether CYP1A1 T3801C polymorphism modulates the relationship between tobacco and alcohol-associated head and neck cancer (HNC) susceptibility among the northeast Indian population. Materials and Methods: One hundred and seventy histologically confirmed HNC cases and 230 controls were included within the study. The CYP1A1 T3801C polymorphism was determined using PCR-RFLP, and the results were confirmed by DNA sequencing. Logistic regression (LR) and multifactor dimensionality reduction (MDR) approaches were applied for statistical analysis. Results: The CYP1A1 CC genotype was significantly associated with HNC risk (P=0.045). A significantly increased risk of HNC (OR=6.09; P<0.0001) was observed in individuals with combined habits of smoking, alcohol drinking and tobacco-betel quid chewing. Further, gene-environment interactions revealed enhanced risks of HNC among smokers, alcohol drinkers and tobacco-betel quid chewers carrying CYP1A1 TC or CC genotypes. The highest risk of HNC was observed among smokers (OR=7.55; P=0.009) and chewers (OR=10.8; P<0.0001) carrying the CYP1A1 CC genotype. In MDR analysis, the best model for HNC risk was the three-factor model combination of smoking, tobacco-betel quid chewing and the CYP1A1 variant genotype (CVC=99/100; TBA=0.605; P<0.0001); whereas interaction entropy graphs showed synergistic interaction between tobacco habits and CYP1A1. Conclusions: Our results confirm that the CYP1A1 T3801C polymorphism modifies the risk of HNC and further demonstrated importance of gene-environment interaction.

• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.5767-5772
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• 2015

Background: Polymorphisms in the MDM2 309 (T>G) and TP53 72 (G>C) genes are reported to increase the susceptibility to head and neck cancer (HNC) in various populations. The risk for HNC is also strongly associated with etiologic habits such as smoking, alcohol consumption and/or chewing of betel quid (BQ). In a case-control study, we investigated the significance of the above polymorphisms alone, and upon interaction with one another as well as with various etiologic habits in determining HNC risk in a Northeast Indian population. Materials and Methods: Genotyping at 309 MDM2 and 72 TP53 in 122 HNC patients and 86 cancer free healthy controls was performed by PCR using allele specific primers, and the results were confirmed by DNA sequencing. Results: Individuals with the GG mutant allele of MDM2 showed a higher risk for HNC in comparison to those with the TT wild type allele (OR=1.9, 95%CI: 1.1-3.3) (p=0.022). The risk was further increased in females by ~4-fold (OR=4.6, 95% CI: 1.1-19.4) (P=0.04). TP53 polymorphism did not contribute to HNC risk alone; however, interaction between the TP53 GC and MDM2 GG genotypes resulted in significant risk (OR=4.9, 95% CI: 0.2-105.1) (p=0.04). Smokers, BQ- chewers and alcohol consumers showed statistically significant and dose-dependent increase in HNC risk, irrespective of the MDM2 genotype. Conclusions: MDM2 genotype could serve as an important predictive biomarker for HNC risk in the population of Northeast India.

• 한국자원식물학회지
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• 제10권2호
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• pp.151-158
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• 1997

본 연구는 콩계통들의 지방함량이 서로 다른 가상요인에 어떻게 영향을 받는 지를 규명하기 위해 미국 노스캐롤라이나지방의 5개 지역에서 3년에 걸쳐 수행하였다. 각 계통의 지방함량 및 지방수량들이 기상요인에 대한 Linear response가 조사되었고, 또한 최저기상 반응모델을 결정하기 위해 Stepwise Selection Program이 사용되었다. 콩지방함량, 지방수량은 기상 요인인 온도와 강우량에 의해 크게 영향을 받았으며 성숙시기에 온도가 가장 높고, 최고최저 온도범위가 가장 작은 환경에서 지방함량이 가장 낮게 나타났고, 최저온도 변이가 가장 작고 강우량변이가 가장 큰 환경에서 지방수량이 가장 높게 나타났다. 계통들의 대부준으 지방함량이 MxDT, HTD, ADT, ADTRg에 반비례하는 경향을 나타내었으며 이는 성숙시기에 온도가 높아질수록 지방함량이 감소한다는 것을 의미한다. 그러나 NC107은 MxDT, ADT에 비례하는 경향을 보였다. 모든 계통들이 VMnDT, VADTRg, ADRa에 비례하는 경향을 나타내었고 이는 최저온도와 최고최저 온도범위의 변이가 클수록, 평균강우량이 많을수록 지방함량은 증가한다는 것을 의미한다. 11계통이 1개 내지 3개의 기상변수를 가지는 최적기상모델을 형성하였다. 그러나 NC109와 NC105는 모든 기상변수에 유의한 Linear 반응을 나타내지는 않았으나 전자는 2개의 기상변수를 가지는 최적기상모델을 형성하였고 후자는 가지지 않았다. 이는 NC109는 지방 함량이 아마도 기상변수 하나에 의한 영향보다도 2개 변수의 상호작용에 의한 영향이 큰 것으로 추측 할 수 있고, NC105는 지방함량이 기상환경에 상대적으로 더 안정하기 때문인 것으로 생각된다. 위와 같은 사실은 콩지방함량의 기상반응에 대한 품종적 차이가 상당히 크다는 것을 의미한다. 지방수량은 ADTRg와 VADRa에 반비례하는 경향을 나타내었으며 이는 최고최저 온도차이와 평균강수량 변이가 클수록 지방수량은 감소한다는 것을 의미한다.