• Journal of Pharmaceutical Investigation
• /
• 제37권3호
• /
• pp.187-192
• /
• 2007

A reversed phase HPLC analysis of atorvastatin (AS) standard solution was performed using diclofenac (DF) as internal standard. Column oven temperature, flow rate and the composition of the mobile phase were varied in order to determine a practical system setup using a C18 column and UV detector. Two C18 columns of different length were compared regarding their influence on the AS peak shape. Based on these preliminary experiments a validation study was performed utilizing a C18 column at $62^{\circ}C$ with a mobile phase consisting of sodium phosphate buffer (0.05 M, pH 4.0), methanol and acetonitrile (40:50:10, v/v/v). The detection limit for AS was $0.1{\mu}g/ml$ and inter- and intra-day calibration curves were linear over a concentration range of $0.2-50{\mu}g/ml$. Accuracy and precision were satisfactory in the AS concentration range of $0.5-50{\mu}g/ml$.

• 천문학회보
• /
• 제39권2호
• /
• pp.119.1-119.1
• /
• 2014

We present the scanned digital images and their preliminary analysis results of objective-prism plates taken with the Michigan Curtis Schmidt telescope at Cerro Tololo Inter-American Observatory. In order to separate an individual spectrum of a star, it should be required to identify each field star in the plate. Using the PPM(Position and Proper Motion) catalog, we are verifying which star corresponds to the spectrum in a plate. In addition to this, we plan to devise the method for wavelength calibration in each spectrum. Finally we will make them for public use, not only for professional astronomers but also for high school and college students for encouraging them to be interested in the astronomy, especially astronomical spectroscopy and stellar astronomy.

• 대한의생명과학회지
• /
• 제21권4호
• /
• pp.241-247
• /
• 2015

Lactate and ketone bodies are considered biological markers for ketosis and several inherited metabolic disorders. In the current study, the specific ratios of lactate and ketone bodies as analytical tools for differential diagnosis of various lactic acidosis were devised. The study included a protein precipitation step following tert-butyldimethylsilyl derivatisation. Total run time was approximately 30 min including sample preparation and GS/MS analysis. The limits of detection were below 0.1 pg/mL over the targeted 4 analytes. The calibration curve was linear over the concentration range of $0.001{\sim}250{\mu}g/mL$ for pyruvate, beta-hydroxybutyrate, and acetoacetate ($R^2$ > 0.99). Inter-day accuracy and precision were 87.7~94.8% with RSD of 2.5~5.7% at 2 levels. Absolute recoveries (%) of target analytes were 87.0~98.4%. The method was validated for the quantification of lactate and ketone bodies for differentiation of lactic acidosis.

• 대한한의학방제학회지
• /
• 제18권2호
• /
• pp.251-257
• /
• 2010

Objectives : To develop and validate High-performance liquid chromatography-photodiode array methods for simultaneous determination of two constituents in Oryeong-san(ORS). Methods : Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array(PDA) detection at 280 nm, were used for quantification of the two marker components of ORS. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was $H_2O$ and solvent B was acetonitrile. Results : Calibration curves were acquired with correlation coefficient ($r^2$)>0.9999, and the relative standard deviation(RSD) values(%) for intra- and inter-day precision were not exceed 1.0%. The recovery rate of each compound was in the range of 93.01-104.16%, with an RSD less than 2.0%. The contents of two compounds in ORS were 1.10-3.72 mg/g. Conclusions : The established HPLC method will be helpful to improve quality control of ORS.

• 약학회지
• /
• 제40권1호
• /
• pp.72-77
• /
• 1996

Penicillin sensor was manufactured by immobillizing penicillinase with glutaraldehyde on the $H^+$-selective membrane based on PVC-COOH-TDDA. This membrane was not inter fered by $K^+$ ion in Pc-G potassium salt. When enzyme was immobilized with glutaraldehyde, the PVC-COOH matrix was more effective than PVC matrix. Calibration curve calculated from Nernst equation was not linear. But potential was relative to concentration of Pc-G. And maximal potentiometric velocity was also relative to concentration of Pc-G. Therefore, it may be applied to Michaelis-Menten equation. The penicillin sensor was useful for determination of Pc-G at concentration of 0.1~10mM level.

• 약학회지
• /
• 제60권1호
• /
• pp.15-20
• /
• 2016

Globotriaosylsphingosine (lyso Gb3) is considered as one of the biomarkers for Fabry disease. A rapid and simple UPLC-MS/MS method was developed for the determination of reliable biomarker, lyso Gb3. Total analytical procedure takes only 15 min including sample preparation and MS/MS analysis. Limit of detection was 0.85 ng/ml (S/N=3). The calibration curve was linear over the range of 2.0~400.0 ng/ml ($R^2=0.9999$). Inter-day and intra-day assay accuracy were 93.4~100.6% (RSD, 0.6~6.0%) and 97.5~100.7% (RSD, 3.6~5.2%). Absolute recoveries of 97.6~98.6 showed excellence of a new analytical method. The method was applied to human and mice urines, proved the suitability for the quantification of lyso-Gb3 for screening, diagnosis and therapeutic monitoring of Fabry disease patients.

• 분석과학
• /
• 제35권2호
• /
• pp.82-91
• /
• 2022

Grayanotoxin-contaminated honey exhibits toxicity. In this study, a reliable and sensitive liquid-chromatography-tandem-mass-spectrometric method (LC-MS/MS) was developed and validated for the quantitation of grayanotoxin I and grayanotoxin III in honey. The grayanotoxins were extracted from honey via solid phase extraction and separated on a biphenyl column with a mobile phase consisting of 0.5 % acetic acid in water and methanol. Mass spectrometric detection was performed in the multiple-reaction monitoring mode with positive electrospray ionization. The calibration curve covered the range 0.25 to 100 ㎍/g. The intra- and inter-day deviations were less than 10.6 %, and the accuracy was between 94.3 and 114.0 %. The validated method was successfully applied to the determination of grayanotoxins in mad honey from Nepal. The concentrations of grayanotoxin I and grayanotoxin III in 33 out of 60 mad honey samples were 0.75 - 64.86 ㎍/g and 0.25 - 63.99 ㎍/g, respectively. The method established herein would help in preventing and confirming grayanotoxin poisoning.

• 한국근적외분광분석학회:학술대회논문집
• /
• 한국근적외분광분석학회 2001년도 NIR-2001
• /
• pp.1510-1510
• /
• 2001

NIRS uses reflectance signals resulting from bending and stretching vibrations in chemical bonds between carbon, nitrogen, hydrogen, sulfur and oxygen. These reflectance signals are used to measure the concentration of major chemical composition and other descriptors of homogenized and freeze-dried whole broiler carcasses. Six strains of chicken were analyzed and the NIRS model predictions compared to reference data. The results of this comparison indicate that NIRS is a rapid tool for predicting dry matter (DM), fat, crude protein (CP) and ash content in the broiler carcass. Males and females of six commercial strain crosses of broiler chicken (Gallus domesticus) were used in this study (6$\times$2 factorial design). Each strain was grown to 16 weeks of age, and duplicate serial samples were taken for body composition analysis. Each whole carcass was pressure-cooked, homogenized, and a representative sample was freeze-dried. Body composition determined as follows: DM by oven dried method at 105$^{\circ}C$ for 3 hours, fat by Mojonnier diethyl ether extraction, CP by measuring nitrogen content using an auto-analyzer with Kjeldhal digest and ash by combustion in a muffle furnace for 24 hour at 55$0^{\circ}C$. These homogenized and freeze-dried carcass samples were then scanned with a Foss NIR Systems 6500 visible-NIR spectrophotometer (400-2500nm) (Foss NIR Systems, Silver Spring, MD., US) using Infra-Soft-International, ISI, WinISl software (ISI, Port Matilda, US). The NIRS spectra were analyzed using principal component (PC) analysis. This data was corrected for scatter using standard normal “Variate” and “Detrend” technique. The accuracy of the NIRS calibration equations developed using Partial Least Squares (PLS) for predicting major chemical composition and carcass descriptors- such as body mass (BM), bird dry matter and moisture content was tested using cross validation. Discrimination analysis was also used for sex and strain identification. According to Dr John Shenk, the creator of the ISI software, the calibration equations with the correlation coefficient, $R^2$, between reference data and NIRS predicted results of above 0.90 is excellent and between 0.70 to 0.89 is a good quantifying guideline. The excellent calibration equations for DM ($R^2$= 0.99), fat (0.98) and CP (0.92) and a good quantifying guideline equation for ash (0.80) were developed in this study. The results of cross validation statistics for carcass descriptors, body composition using reference methods, inter-correlation between carcass descriptors and NIRS calibration, and the results of discrimination analysis for sex and strain identification will also be presented in the poster. The NIRS predicted daily gain and calculated daily gain from this experiment, and true daily gain (using data from another experiment with closely related broiler chicken from each of the six strains) will also be discussed in the paper.

• 대한전자공학회논문지SP
• /
• 제47권6호
• /
• pp.127-136
• /
• 2010

다시점 비디오는 카메라간의 다른 위치와 불완전한 카메라 보정(calibration)으로 인접한 시점의 영상 내에 존재하는 동일물체 간에 색상 차이가 발생할 수 있다. 이러한 색상 불일치(color mismatch)는 시점 간 움직임 예측(inter-view prediction) 수행 시, 오정합을 발생시켜 다시점 비디오 부호화(Multi-view Video Coding : MVC) 성능을 저하시키는 원인이 된다. 본 논문에서는 이웃하는 영상 간에 존재하는 휘도 및 색차 성분 불일치를 보상하여 다시점 비디오 부호화의 압축률을 향상시키는 전처리 기법을 제안한다. 제안된 기법에서는 모든 시점의 영상을 히스토그램 매칭 기법에 의해 정해진 참조 시점 영상의 색상을 기준으로 보정된다. 또한 히스토그램 매칭 수행 전에 YCbCr 색상공간 변경 시에 색차 성분의 대표 값 추출(chrominance subsampling)에 사용되는 Cosited filter를 영상의 각 색상성분에 적용하여 성능을 더욱 높일 수 있다. 히스토그램 매칭은 YCbCr 색상공간에서 RGB 색상공간으로 변환하여 각 색상성분에 적용한다. 이 과정에서 영상에 존재하는 에지의 방향성과 화소 값의 존재 범위를 고려한 효과적인 색상 변환 기법이 사용된다. 실험을 통해 제안하는 전처리 기법이 다른 기법들에 비해 향상된 부호화 효율을 가지는 것을 확인하였다.

• 방송공학회논문지
• /
• 제15권6호
• /
• pp.768-782
• /
• 2010

다시점 비디오의 조명 불일치 현상은 서로 다른 카메라의 위치와 카메라간의 불완전한 보정(calibration)으로 인하여 발생한다. 이러한 인접 시점간의 색상 불일치는 획득된 영상을 참조 영상으로 이용하여 부호화하는 다시점 비디오 부호화(multi-view video coding)의 성능을 저하시키는 요인이 된다. 이러한 조명 불일치를 보상하기 위한 방법 중에서 히스토그램 매칭(histogram matching)을 이용한 전처리 기법이 있다. 히스토그램 매칭을 통해 모든 시점 영상의 히스토그램은 정해진 참조 시점 영상의 히스토그램으로 매칭되어지고 다시점 비디오 부호화의 성능을 개선할 수 있다. 그러나 다시점 비디오 시퀀스는 카메라와 등장인물의 이동으로 인하여 시점 간 영상뿐만 아니라, 한 시점 내에 시간의 흐름에 따른 영상간의 히스토그램 분포가 서로 다를 수 있다. 참조 시점 시퀀스에 속한 모든 영상을 참조하는 기존의 히스토그램 매칭 기법은 시공간적으로 상관성이 높지 않은 영상의 조명을 효과적으로 보상하기에 적합하지 않다. 본 논문에서는 시점 영상 간의 색상 분포의 차이를 보이는 다시점 비디오를 보상하여 공간적 상관성을 높이기 위해 두 조건식이 반영된 영상분리 기법을 적용한 레이어별 히스토그램 매칭 기법과 시간의 흐름에 따라 색상 분포의 차이를 보이는 다시점 비디오를 비디오 부호화의 단위인 화면 그룹(group of pictures : GOP)별로 보상하여 시간적 상관성을 높이는 개별적인 히스토그램 매칭 기법을 제안한다. 실험을 통해 제안하는 조명 보상 기법이 기존의 조명 보상 기법보다 향상된 다시점 비디오 부호화 효율을 보이는 것을 확인하였다.