• Journal of Microbiology and Biotechnology
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• 제21권6호
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• pp.574-581
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• 2011

A Gram-positive bacterium was isolated from the saline soils of Jangpura (U.P.), India, and showed high-level of radiation-resistant property and survived upto 12.5 kGy dose of gamma radiation. The 16S rDNA sequence of this strain was examined, identified as Bacillus sp. strain HKG 112, and was submitted to the NCBI GenBank (Accession No. GQ925432). The mechanism of radiation resistance and gene level expression were examined by proteomic analysis of whole-cell extract. Two proteins, 38 kDa and 86.5 kDa excised from SDS-PAGE, which showed more significant changes after radiation exposure, were identified by MALDI-TOF as being flagellin and S-layer protein, respectively. Twenty selected 2-DE protein spots from the crude extracts of Bacillus sp. HKG 112, excised from 2- DE, were identified by liquid chromatography mass spectrometry (LC-MS) out of which 16 spots showed significant changes after radiation exposure and might be responsible for the radiation resistance property. Our results suggest that the different responses of some genes under radiation for the expression of radiation-dependent proteins could contribute to a physiological advantage and would be a significant initial step towards a fullsystem understanding of the radiation stress protection mechanisms of bacteria in different environments.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2001년도 한국생물과학협회 학술발표대회
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• pp.153.2-154
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• 2001

No Abstract, See Full Text

• 대한한의학원전학회지
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• 제21권2호
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• pp.29-37
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• 2008

Based on 11 Classics of Materia Medica designated by Ministry of health and welfare bulletin 1995-15 as legal basis in scope and preparation of herbal prescription, we reviewed indication, property and processing of Rhus Verniciflua Stokes which appear extensively in literature since earlier Joseon Dynasty. Following is the conclusion of this review after comparing with 'Korean Pharmacopoeia: commentary on herbal product standard'. 1. The properties of Rhus Verniciflua Stokes in 'Korean Pharmacopoeia :commentary on herbal product standard' should be changed as 'Tonifying the middle, restoring the muscle, fulfilling marrow, breaking old static blood, tonifying and activating after removing mass, unblocking the meridian, killing worms' and indication should be changed as 'Old static blood, deep rooted mass, wind-cold-dampness arthralgia, cough, 9 types of chest pain, abdominal or flank accumulation of stagnated qi[氣], amenorrhea, hernia mass, small bowel or bladder colic pain, abdominal pain due to worm accumulation'. 2. The processing of Rhus Verniciflua Stokes in 'Korean Pharmacopoeia: commentary on herbal product standard' should include 'natural drying or steaming drying followed by grinding and stir-baking until ripened or smoking appears'.

• 대한통합의학회지
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• 제7권2호
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• pp.11-17
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• 2019

Purpose : The purpose of this study was to determine the effects of specific frequency and application timing of microcurrent (MC) on the mechanical property of muscle caused by delayed onset muscle soreness (DOMS). Methods : The subjects were 32 healthy adults with 8 subjects randomly assigned to four groups (I; 40 Hz MC while inducing DOMS, II; 40 Hz MC immediately after inducing DOMS, III; 284 Hz MC while inducing DOMS, IV; 284 Hz MC immediately after inducing DOMS). DOMS is applied to the biceps brachii muscle while MC was applied at an intensity of $300{\mu}A$ for 10 minutes. The mechanical properties of muscle were measured before and immediately after DOMS. Results : In terms of muscle tone, there were significant differences in interaction effects between time and groups. Regarding muscle elasticity and stiffness, there were no significant differences in interaction effects between time and groups but there were only significant differences in main effects based on time. Conclusion : The results indicated that 40 Hz MC had an effect on reducing muscle tone regardless of application timing. However, both 40 Hz and 284 Hz MC did not trigger changes in muscle elasticity and stiffness regardless of application timing.

• Journal of Ginseng Research
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• 제47권3호
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• pp.385-389
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• 2023

Background: Ginseng has been used as a traditional medicine for treatment of many diseases and for general health maintenance. Previously, we showed that ginseng did not demonstrate estrogenic property in ovariectomized mouse model. However, it is still possible that disruption of steroidogenesis leading to indirect hormonal activity. Methods: The hormonal activities were examined in compliance with OECD guidelines for detecting endocrine disrupting chemicals: test guideline (TG) No. 456 (an in vitro assay method for detecting steroidogenesis property) and TG No. 440 (an in vivo short-term screening method for chemicals with uterotrophic property). Results: Korean Red Ginseng (KRG) and ginsenosides Rb1, Rg1, and Rg3 did not interfere with estrogen and testosterone hormone synthesis as examined in H295 cells according to TG 456. KRG treatment to ovariectomized mice did not show a significant change in uterine weight. In addition, serum estrogen and testosterone levels were not change by KRG intake. Conclusion: These results clearly demonstrate that there is no steroidogenic activity associated with KRG and no disruption of the hypothalamic-pituitary-gonadal axis by KRG. Additional tests will be performed in pursuit of cellular molecular targets of ginseng to manifest mode of action.

• 한국해양바이오학회지
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• 제11권2호
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• pp.36-41
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• 2019

This study was conducted to analyze the difference in mechanically improved properties by distinguishing α-chitin and β-chitin for Poly(L-lactic acid)(PLLA). First, dissolution of chitins was established by mixing polar solvents hexafluoroisopropanol (HFIP) and trifluoroacetic acid (TFA) in appropriate proportions. Under these conditions, the dissolved chitin was used for electrospinning with other polymers. The electrospun nanofibers of the PLLA and chitins were successfully produced. Compared to the pristine state, when chitin was added to PLLA, the tensile strength increased 1.41 times (α-chitin), by 1.61 times (β- chitin), respectively. Based on this, it was confirmed that α- and β- chitin could be strategically used for different polymers. The results also suggest that chitin can be applied to various fields as good reinforcing material as well as electrospinning.

• Animal cells and systems
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• 제12권1호
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• pp.41-46
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• 2008

A bacterium strain called Bacillus coagulans was isolated from an industrial wastewater drainage and selected for its antimicrobial activities against bacteria and fungi. Characterization studies strongly suggested that this strain is Bacillus coagulans. Antimicrobial activity was found against gram-positive, gram-negative bacteria and yeast strain. Maximal activity was observed after 24 h when incubated at $30^{\circ}C$ and pH 8. The activity was found to be stable at $75^{\circ}C$ for 30 min and at pH range of 2-12. Analysis of the antimicrobial compound by SDS-PAGE suggested a molecular mass of approximately 7.5 KDa. The substance was characterized as a bacteriocin, because of its proteinaceous nature and low molecular weight. Our bacteriocin could potentially be used as a food preservative, because of its thermostable property and broad antimicrobial spectrum.

• 가정과삶의질연구
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• 제4권2호
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• pp.67-88
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• 1986

This study attempts to explain the income and consumption problems of urban low-income consumers and to explore factors of their poverty. For the purpose of this study, interviews were conducted with 124 low-income home managers. All the subjects live in Seoul and Inchon. The major findings are: First, low-income families seriously suffer from low income and small property. And the degree of economic hardship is the most serious with wife-working families whose husbands have no job or are dead. Second, low-income consumers have low budgeting and purchasing skill. Third, low-income consumers have 'doing' orientation but have fatalistic value orientation. This study suggests that it is necessary to develop an integrative measurement of economic hardship. It also suggests that low level of low-income consumer's skill can be well explained in terms of man-nature value orientation.

• Genomics & Informatics
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• 제16권3호
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• pp.65-70
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• 2018

The non-coding DNA in eukaryotic genomes encodes a language which programs chromatin accessibility, transcription factor binding, and various other activities. The objective of this short report was to determine the impact of primary DNA sequence on the epigenomic landscape across 200-base pair genomic units by integrating nine publicly available ChromHMM Browser Extensible Data files of the Encyclopedia of DNA Elements (ENCODE) project. The nucleotide frequency profiles of nine chromatin annotations with the units of 200 bp were analyzed and integrative Markov chains were built to detect the Markov properties of the DNA sequences in some of the active chromatin states of different ChromHMM regions. Our aim was to identify the possible relationship between DNA sequences and the newly built chromatin states based on the integrated ChromHMM datasets of different cells and tissue types.

• 한국동물학회지
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• 제38권2호
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• pp.212-219
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• 1995

Through the histolosical observation of ovary in the nine species of the genus Cobitis, we found that specific adhesive membranes were attached to the outer zone radials during the vitellosenesis. These adhesive membranes could be classified into four forms as follows: 1) granular form of Colitis lutheri, C. striate, and C. sinensis, 2) villous form of C. longicorpus, C. konensis koreensis, C. k. pumilus, and C. granoei, 3) saw-like form of C. rotundicaudata, and 4) filamentous form of C. chou. The various forms of adhesive membrane of egg in the cobitid fishes are showed a species specificitv with reference to their habitats and spawning property.