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Development and validation of analytical methods for pyrifluquinazon residues determination on agricultural commodities by HPLC-UVD (HPLC-UVD를 이용한 농산물 중 pyrifluquinazon 잔류시험법 개발 및 검증)

  • Do, Jung-Ah;Kwon, Ji-Eun;Kim, Mi-Ra;Lee, Eun-Mi;Kuk, Ju-Hee;Cho, Yoon-Jae;Chang, Moon-Ik;Kwon, Kisung;Oh, Jae-Ho
    • Analytical Science and Technology
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    • v.26 no.3
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    • pp.174-181
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    • 2013
  • Pyrifluquinazon is classified with a quinazoline insecticide that regulates food intake by controling the feeding behavior acting on the endocrine or nervous system of pests such as aphids and white fly. To keep safety on pyrifluquinazon residues in agricultural commodities a simple, accurate and rapid analytical method was developed and validated using high performance liquid chromatograph (HPLC-UVD). The pyrifluquinazon residues acidified with 1% formic acid in samples were extracted with acetonitrile and partitioned with hexane subsequently to dichloromethane then purified with silica solid phase extraction (SPE) cartridge. The purified samples were detected using HPLC-UVD. The method was validated using apple and pear spiked with pyrifluquinazon at 0.02, 0.05 and 0.1 mg/kg and hulled rice, pepper, soybean at 0.05 and 0.1 mg/kg. Average recoveries were 70.5~107.9% with relative standard deviation less than 10%. The result of recoveries and overall coefficient of variation of a laboratory results in Gwangju regional FDA and Daejeon regional FDA was followed with Codex guideline (CODEX CAC/GL 40). This method is appropriated at pyrifluquinazon residues determination and will be used as official method of analysis.

Cashew reject meal in diets of laying chickens: nutritional and economic suitability

  • Akande, Taiwo O;Akinwumi, Akinyinka O;Abegunde, Taye O
    • Journal of Animal Science and Technology
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    • v.57 no.5
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    • pp.17.1-17.6
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    • 2015
  • The present study investigated the nutritional and economic suitability of cashew reject meal (full fat and defatted) as replacement for groundnut cake (GNC) in the diets of laying chickens. A total of eighty four brown shavers at 25 weeks of age were randomly allotted into seven dietary treatments each containing 6 replicates of 2 birds each. The seven diets prepared included diet 1, a control with GNC at $220gkg^{-1}$ as main protein source in the diet. Diets 2, 3 and 4 consist of gradual replacement of GNC with defatted cashew reject meal (DCRM) at 50%, 75% and 100% on weight for weight basis respectively while diets 5, 6 and 7 consist of gradual inclusion of full fat cashew reject meal (FCRM) to replace 25%, 35% and 50% of GNC protein respectively. Each group was allotted a diet in a completely randomized design in a study that lasted eight weeks during which records of the chemical constituent of the test ingredients, performance characteristics, egg quality traits and economic indicators were measured. Results showed that the crude protein were 22.10 and 35.4% for FCRM and DCRM respectively. Gross energy of DCRM was 5035 kcal/kg compared to GNC, 4752 kcal/kg. Result of aflatoxin $B_1$ revealed moderate level between 10 and $17{\mu}g/Kg$ in DCRM and GNC samples respectively. Birds on control gained 10 g, while those on DCRM and FCRM gained about 35 g and 120 g respectively. Feed intake declined (P < 0.05) with increased level of FCRM. Hen day production was highest in birds fed DCRM, followed by control and lowest value (P < 0.05) was recorded for FCRM. No significant change (P > 0.05) was observed for egg weight and shell thickness. Fat deposition and cholesterol content increased (P > 0.05) with increasing level of FCRM. The cost of feed per kilogram decreased gradually with increased inclusion level of CRM. The prediction equation showed the relative worth of DCRM compared to GNC was 92.3% whereas the actual market price of GNC triples that of DCRM. It was recommended that GNC could be completely replaced by DCRM in layer's diets in regions where this by product is abundant. However, FCRM should be cautiously used in diets of laying chickens.

Effects of varying nursery phase-feeding programs on growth performance of pigs during the nursery and subsequent grow-finish phases

  • Lee, Chai Hyun;Jung, Dae-Yun;Park, Man Jong;Lee, C. Young
    • Journal of Animal Science and Technology
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    • v.56 no.7
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    • pp.24.1-24.6
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    • 2014
  • The present study investigated the effects of varying durations of nursery diets differing in percentages of milk products on growth performance of pigs during the nursery phase (NP) and subsequent grow-finish phase (GFP) to find the feasibility of reducing the use of nursery diets containing costly milk products. A total of 204 21-d-old weanling female and castrated male pigs were subjected to one of three nursery phase feeding programs differing in durations on the NP 1 and 2 and GFP diets containing 20%, 7%, and 0% lacrosse and 35%, 8%, and 0% dried whey, respectively, in 6 pens (experimental units) for 33 d: HIGH (NP 1, 2 and 3 diets for 7, 14, and 12 d), MEDIUM (NP 2 and 3 for 14 and 19 d), and LOW (NP 2 and 3 and GFP 1 for 7, 14, and 12 d). Subsequently, 84 randomly selected pigs [14 pigs (replicates)/pen] were fed the GFP 1, 2 and 3 diets during d 54-96, 96-135, and 135-182 of age, respectively. The final body weight (BW) and average daily gain (ADG) of nursery pigs did not differ among the HIGH, MEDIUM, and LOW groups (14.8, 13.3, and 13.7 kg in BW and 273, 225, and 237 g in ADG, respectively). The average daily feed intake during the nursery phase was greater (p < 0.01) in the HIGH group than in the MEDIUM and LOW groups, whereas the gain:feed ratio did not differ across the treatments. The BW on d 182 and ADG during d 54-182 were greater in the HIGH and MEDIUM groups vs. the LOW group (110.0, 107.6, and 99.6 kg in BW, respectively; p < 0.01). The backfat thickness and carcass grade at slaughter on d 183 did not differ across the treatments. In conclusion, the MEDIUM program may be inferior to the commonly used HIGH program in supporting nursery pig growth. Nevertheless, the former appears to be more efficient than the latter in production cost per market pig whereas the LOW program is thought to be inefficient because of its negative effect on post-nursery pig growth.

Effects of Dietary Energy Level on Growth and Meat Quality of Korean Black Goats (사료의 에너지 수준이 육성기 흑염소의 발육과 육질에 미치는 영향)

  • Choi, S.H.;HwangBo, S.;S. W. Kim;Kim, Y.K.;Sang, B.D.;Myung, J.H.;Hur, S.N.;Jo, I.H.
    • Journal of Animal Science and Technology
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    • v.49 no.4
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    • pp.509-514
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    • 2007
  • This study was conducted to investigate the optimal energy level at growing Korean black goat from April 20 to November 6, 2006. Forty male Korean black goat were divided into four treatments and were fed with concentrate feed containing ME 2.4, 2.6, 2.8 and 3.0 Mcal/kg with CP 15% and rice straw. Average daily gains of were 59.8, 65.4, 73.2 and 77.2 g/day in groups fed with ME 2.4, 2.6, 2.8 and 3.0 Mcal/kg diet, respectively. There was a tendency to increase ADG as the ME level of concentrate feed grew. Dressing percentage were 49.73, 48.64, 50.19 and 51.36% in each group, and were increased as the ME level grew. Meat percentage ranged between 53.1 and 55.3%, and were almost constant. Ranges of crude fat contents and water holding capacity were and 57.9~59.0%, which were increased as the ME level grew. On the other hand, shear force were between 2.8 and 3.1 kg/cm2, which were decreased as the ME level grew. These results suggest that the optimal energy level at growing goat's concentrate is ME 3.0 Mcal/kg.

Removal effect of Cadmium by Polyphenol Compound Extracted from Persimmon Leaves(Diospyros kaki folium) (감잎(Diospyros kaki folium)으로부터 분리한 Polyphenol 화합물의 카드뮴 제거효과)

  • Jo, Guk-Young;Choi, Hee-Jin;Son, Jun-Ho;Bae, Du-Kyung;Park, Mu-Hee;Woo, He-Sob;An, Bong-Jeon;Bae, Man-Jong;Choi, Cheong
    • Applied Biological Chemistry
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    • v.43 no.3
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    • pp.213-217
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    • 2000
  • In order to investigate the removal effect of cadmium by polyphenol compound extracted from persimmon leaves(Diospyros kaki folium), animal test was done. Two fractions such as F-1 and F-2 from persimmon leaves were compared with their safety and function. The food intake of group F-1 and F-2 considerably decreased within 1% level. Cadmium addition influenced to rat growth(a tested animal), but food efficiency ratio(FER) wasn't shown any considerable difference in F-1 and F-2, as compared to the control. Cadmium content of liver, kidney and femur considerably decreased within 1% level in F-1 and F-2, compared to the control, cadmium content of liver decreased 25% in F-1, 28% in F-2 3150 decreased 22% md 25% in kidney. In femur, also decreased 53% in F-1 and 59% in F-2 respectively. The test of cadmium content in feces indicate that the content considerably increased within 1% level in both group F-1 and F-2, as compared to the control(42% in F-1, 54% in F-2). As shown the above results, have seen the removal effect of cadmium by polyphenol compound.

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Estimating urinary energy value of rat from the urinary nitrogen content (쥐의 뇨질소함량(尿窒素含量)으로부터 뇨(尿) Energy 가(價)의 산출법(算出法))

  • Han, In-K.
    • Applied Biological Chemistry
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    • v.7
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    • pp.29-33
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    • 1966
  • This experiment was conducted to study the relationship between the energy value and the nitrogen content in the rat urine. Thirteen rats for ad libitum feeding group and 16 rats for two-meal group were employed in this experiment. The experimental period consisted of 22 days with a preliminary period of 10 days. During the last eight days of the experiment the urine was collected quantitatively. The rats fed two-meal per day excreted significantly (p<0.005) more nitrogen and energy in the urine than those fed ad libitum. A linear relationship between the energy concentration and nitrogen content was found. The urinary energy value was increased as the urinary nitrogen content was increased. A prediction equation was derived to compute the energy value from the content of nitrogen as follows: Y=8.924X+0.182 $S_{y{\cdot}x}=0.788$ where Y=urinary energy(kcal/100 ml) X=urinary nitrogen(gm/100 ml) Since the standard deviation of estimate is in small magnitude (0.788 kcal) when it is compared with the amount of intake of gross energy, digestible energy or metabolizable energy, this equation can be used safely to estimate the energy value from nitrogen content. Consequently, considerable amound of time and labor for the actual determination of energy can be saved. The ratio of energy to nitrogen was found to be 9.4 for ad adlibitum group and 8.6 for two-meal group. No significant difference between two group in this respect was observed.

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Monitoring and Risk Assessment of Ethyl Carbamate in Korean Major Foods (다소비 식품 중, ethyl carbamate의 분포측정 및 위해성 평가)

  • Hong, Kwon-Pyo;Roh, I-Woo;Kang, Yoon-Seok;Jung, Dong-Chae;Park, Sae-Rom;Yoon, Ji-Ho;Bae, Dong-Ho
    • Applied Biological Chemistry
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    • v.50 no.1
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    • pp.29-35
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    • 2007
  • Levels of ethyl carbamate, a potential carcinogen produced naturally during fermentation, in major Korean fermented foods and some selected alcoholic beverages were determined by GC/MS/SIM, and their average daily intake and excess cancer risk of Korean people were estimated. In GC/MS/SIM analysis average 1.41, 19.44, 3.00 and 170.88 ${\mu}$g/kg of ethyl carbamates were detected in Baechu kimchi, Japanese soy sauces, Soju and fruit brandy, respectively. The average and maximum daily exposures to ethyl carbamate through major Korean fermented foods and alcoholic beverage consumption were 9.42 and 35.75 ng/kg bw/day for Korean female aged 20-64 years, and 12.73 and 48.87 ng/kg bw/day for Korean male respectively, which were mainly contributed by Baechu kimchi, Japanese soy sauces, Soju and fruit brandy. The average and maximum excess cancer risks were $4.7{\times}10^{-7}$ and $1.8{\times}10^{-6}$ for Korean female, and $6.4{\times}10^{-7}$ and $2.4{\times}10^{-6}$ for Korean male. Therefore, the industries related to fermented foods and alcoholic beverages need to make an effort to reduce the amount of ethyl carbamate in their products.

According to the BMI of Children and Adolescents, Eating-Lifestyle Habits, and Recognized Study (아동·청소년의 BMI에 따른 식생활습관 및 인식 연구)

  • Lee, Yeo Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.1
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    • pp.265-274
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    • 2013
  • The purpose of this study was to investigate the dietary habits of the relevant factors according to the obesity status of children and adolescents. Services research studies conducted in the Ministry of Health, Welfare and Family Affairs of the '2009 Children and Youth Online Games and Family Activity Survey findings utilizing data. Study, the subjects utilized 9,462 elementary school students 2,778 people, 3,385 middle school students, high school students a total of 3,299 people were enrolled in the study. Were grouped according to the BMI of the study subjects. After dinner snack intake 18.5>BMI groups disagree 38.8%, 18.5${\leq}$BMI<23.0 group it was 41.1%, 23.5${\leq}$BMI<25.0 group it was 40.1%, $25{\leq}BMI$ groups disagree 37.4% response was highest(p=0.001). Investigate whether regular exercise every day, results showed that a high BMI index groups do not exercise more. Aggressiveness in time, such as physical education, the survey showed that a low BMI index group more likely to exercise vigorously. BMI index group of people around you and the speed of eating more meals were faster. If you let more to allow time to practice proper education of children and youth, health and enrich lives of the appropriation will be able to Services to live a healthy and happy life than to correct perceptions and lifestyles of children and adolescents. To do this, there is a need to educate parents, children and youth, as well as.

Effects of Feeding Lactic Acid Bacteria-Based Direct-Fed Microbial Complex on Growth Performance and Blood Characteristics in Growing Pigs (Lactic acid bacteria 계열의 복합 생균제가 육성돈의 성장 능력과 혈액 특성에 미치는 영향)

  • Yoo, Jong-Sang;Kim, In-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.7 no.6
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    • pp.1416-1420
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    • 2006
  • This study was conducted to investigate the effects of direct-fed microbial(DFM) complex on the growth performance and blood characteristics in growing pigs. A total of 72 growing pigs with an average initial BW of 24.64$\pm$2.46 kg were used in 28 days experiment trial. There were six pens per treatment with three pigs per pen. Dietary treatments included 1)NC(basal diet; antibiotics free diet), 2) PC(NC diet with 0.1% antibiotics; chlortetracycline 0.05% +neomycin 0.05%), 3)DFM0.1 UC diet + 0.1% DFM)and 4)DFM0.3 (NC diet + 0.3% DFM). During the entire experimental period, hnG(Average daily gain) was increased NC treatment compared with NDFM0.1 and NDFM0.3 treatments. However, there was not significantly effect by treatments(P>0.05). Also, there was not significantly effect in ADFI(average daily feed intake) among the treatments. Blood characteristics(RBC, WBC and IgG) tended to improved, however, no significant differences were observed(P>0.05). In our experiment, DFM had not effects on growth performance and blood characteristics in growing pigs.

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A Numerical Study on Performance of a Heavy-Duty Diesel engine for Power Generation under Natural Gas-Diesel Dual Fuel Operation (발전용 대형 디젤 엔진의 천연가스-디젤혼소 운전 특성에 대한 수치해석 연구)

  • Cho, Jungkeun;Park, Sangjun;Song, Soonho;Hur, Kwang-Beom
    • Journal of the Korean Institute of Gas
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    • v.19 no.2
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    • pp.29-36
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    • 2015
  • This study is an 1-D numerical study prior to modification of diesel engine for power plants to natural gas/diesel dual fuel engine using GT-Power with 1.5MW diesel engine for power generation. Natural gas injector was installed to intake manifold for dual fuel engine model. Effects on engine performance and characteristics were investigated when dual fuel is used in unmodified diesel engine. The analysis was done under 5 conditions from 0% to 40% of mixing rate on 720RPM engine speed. As a result of research, the engine performance was decreased as increasing ratio of natural gas. Engine brake power was decreased by 18.4% under 40% mixing rate condition. To clarify the reason, effects of injection timing and period were evaluated with DOE method. Considering this result, optimization was done for these parameters. Also, comparison between performances of dual fueled engine and diesel engine was made after optimizing the timing of injection by DOE method. As a result, engine brake power was decreased by 8.55% under mixing rate 40% condition showing 12.5% improvement.