• 대한본초학회지
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• 제29권5호
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• pp.31-38
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• 2014

Objectives : Egg yolk is composed of various important chemical substances for human health. A calcium shortage causes the growth retardation on the body growth. In this study, we examined the therapeutic effects of calcium, vitamin D and egg yolk peptide (EYP) treatment on the retardation of the longitudinal bone growth induced by low-calcium diet in adolescent rats. Methods : Low calcium diets were administrated for 15 days. During the last five days, calcium and/or vitamin D and/or EYP were administrated. The body weights, longitudinal bone growth rates, the heights of growth plates, and bone morphogenetic protein (BMP)-2 and insulin-like growth factor (IGF)-1 expressions were measured using histochemical analysis. Results : Low calcium diets caused the significant reduction in body weight gains and the longitudinal bone growth. The heights of growth plates and the expressions of BMP-2 and IGF-1 showed the impairment of body growth as well. Calcium and/or vitamin D administration could not significantly increase the longitudinal bone growth. However, calcium, vitamin D, and EYP administration significantly increased the bone growth, the growth plate height, and BMP-2 and IGF-1 expressions. Conclusions : These results suggest that EYP enhances the longitudinal bone growth in the calcium and/or vitamin D deficiency and it could be a promising agent for the treatment of children suffering from malnutrition.

• 대한한의학회지
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• 제33권2호
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• pp.1-10
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• 2012

Objectives: This study was aimed to investigate the effect of Bojungikgitang-gagambang (BJIG) on longitudinal bone growth in rats. Methods: The BJIG treated group (300 mg/kg) and the control group (vehicle) were administered orally twice daily for 4 days. To investigate the effects of BJIG we measured body weight gain. The bone growth effect was analyzed by measuring between fluorescent lines marked with tetracycline, which plays the role of fluorescent dye on the surface of the tibia. Tetracycline was intraperitoneally injected. The height of growth plates in the epiphyseal plate was measured. The expression of bone morphogenetic protein-2 (BMP-2) and insuline-like growth factor-1 (IGF-1) was investigated by immunohistochemistry. Results: BJIG caused a significant acceleration of longitudinal bone growth of $349.7{\pm}15.9{\mu}m/day$ compared to control ($319.8{\pm}21.4{\mu}m/day$). The height of overall growth plate was not significantly more compared to the control, but the size of cells in the proliferative zone and hypertrophic zone were. In the immunohistochemistry, BMP-2 and IGF-1 were expressed markedly in the proliferative or hypertrophic zone, respectively. Conclusions: BJIG stimulated the chondrocyte hypertrophy and chondrogenesis in the growth plate and directly increased the longitudinal tibia length of rats.

• 한국가축번식학회지
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• 제22권4호
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• pp.341-347
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• 1998

본 연구는 생후 33 주령부터 초발정이 시작될 때까지 한우육성빈우의 일당증체량 (0.5kg, 0.7kg) 에 따른 혈중 progesterone 과 IGF-1 농도를 분석하여 한우의 초발정시기 및 춘기발동기전후 혈중 progesterone 과 IGF-1 농도를 비교 검토하기 위하여 수행하였다. 모든 시험축에서, 초발정 직전까지는 혈중 progesterone 농도는 검출할 수 없었으며, 13 두의 육성빈우에서 첫발정 6일전에 미미한 혈중 progesterone 농도가 상승하기 시작하여 짧은 황체기를 보였다. 초발정시기의 일당증체량 0.7kg 구의 평균 나이는 331.0$\pm$15.0 일로 0.5kg 구의 358.9$\pm$7.9 일보다 빨랐고 이때 체중도 0.7kg 구가 236.0$\pm$4.7kg으로 0.5kg 구의 224.8$\pm$9.7 보다 무거웠다. 초발정이후 3~15 일까지 IGF-1 농도는 황체초기 (0~1) 와 황체퇴행기 (18일~20일) 보다 높은 경향을 보였고, 성장단계에 따라서 생후 37주령부터 57주령까지 조사한 IGF-1 농도 변화는 37주령에 93.2$\pm$63.4ng/$m\ell$ 에서 57 주령에 170.9$\pm$59.35ng/$m\ell$로 점차적으로 상승하였다. 이상의 결과 한우의 초발정 평균나이는 345일령 전후로 추정되며 이시기에 혈중 progesterone 농도는 IGF-l 농도와 밀접한 관련이 있는 것 같다.