• The Korea Journal of Herbology
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• v.29 no.5
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• pp.31-38
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• 2014

Objectives : Egg yolk is composed of various important chemical substances for human health. A calcium shortage causes the growth retardation on the body growth. In this study, we examined the therapeutic effects of calcium, vitamin D and egg yolk peptide (EYP) treatment on the retardation of the longitudinal bone growth induced by low-calcium diet in adolescent rats. Methods : Low calcium diets were administrated for 15 days. During the last five days, calcium and/or vitamin D and/or EYP were administrated. The body weights, longitudinal bone growth rates, the heights of growth plates, and bone morphogenetic protein (BMP)-2 and insulin-like growth factor (IGF)-1 expressions were measured using histochemical analysis. Results : Low calcium diets caused the significant reduction in body weight gains and the longitudinal bone growth. The heights of growth plates and the expressions of BMP-2 and IGF-1 showed the impairment of body growth as well. Calcium and/or vitamin D administration could not significantly increase the longitudinal bone growth. However, calcium, vitamin D, and EYP administration significantly increased the bone growth, the growth plate height, and BMP-2 and IGF-1 expressions. Conclusions : These results suggest that EYP enhances the longitudinal bone growth in the calcium and/or vitamin D deficiency and it could be a promising agent for the treatment of children suffering from malnutrition.

• The Journal of Korean Medicine
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• v.33 no.2
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• pp.1-10
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• 2012

Objectives: This study was aimed to investigate the effect of Bojungikgitang-gagambang (BJIG) on longitudinal bone growth in rats. Methods: The BJIG treated group (300 mg/kg) and the control group (vehicle) were administered orally twice daily for 4 days. To investigate the effects of BJIG we measured body weight gain. The bone growth effect was analyzed by measuring between fluorescent lines marked with tetracycline, which plays the role of fluorescent dye on the surface of the tibia. Tetracycline was intraperitoneally injected. The height of growth plates in the epiphyseal plate was measured. The expression of bone morphogenetic protein-2 (BMP-2) and insuline-like growth factor-1 (IGF-1) was investigated by immunohistochemistry. Results: BJIG caused a significant acceleration of longitudinal bone growth of $349.7{\pm}15.9{\mu}m/day$ compared to control ($319.8{\pm}21.4{\mu}m/day$). The height of overall growth plate was not significantly more compared to the control, but the size of cells in the proliferative zone and hypertrophic zone were. In the immunohistochemistry, BMP-2 and IGF-1 were expressed markedly in the proliferative or hypertrophic zone, respectively. Conclusions: BJIG stimulated the chondrocyte hypertrophy and chondrogenesis in the growth plate and directly increased the longitudinal tibia length of rats.

• Korean Journal of Animal Reproduction
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• v.22 no.4
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• pp.341-347
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• 1998

This study was conducted to determine changes in circulating progesterone and Insulin-like Growth Factor-1(IGF-1) concentrations according to daily gain of 0.5 and 0.7 kg ; from 33 weeks of age until the onset of regular oestrus cycles in the heifer of Hanwoo. In all animals, progesterone concentrations was undetectable until just before first oestrus. A small progesterone elevation of approximately 6 days duration(based upon the mean progesterone profile) preceded the first oestrus in 13 heifers. The mean age at first oestrus of the daily gain 0.7 kg(331.0$\pm$ 15.0 days) group was earlier than those of the daily gain 0.5 kg(358.9$\pm$7.9 days) group, and the mean weight at first oestrus of the daily gain 0.7 kg(236.0$\pm$4.7 kg) group was heavier than those of the daily gain 0.5 kg(224.8$\pm$9.7 kg) group. IGF-1 concentrations from day 3 to 15 of first oestrus were higher than those of the earlier luteal stage(Day 0~1) and the luteal regression stage (Day 18~20). IGF-1 concentrations according to the growth stage (from 33 weeks to 57 weeks) increased gradually. On the basis of these results, we suggest that the onset of first oestrus in Hanwoo heifer is about 345 day of age, and progesterone concentrations are closely related to the IGF-1 concentration.