• Journal of agriculture & life science
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• v.46 no.4
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• pp.9-20
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• 2012

Eugenol is a volatile compound synthesized by eugenol synthase in various plants and belongs to phenylpropene compounds. However, characteristics of eugenol synthase in tomato has not been known. Therefore, we cloned a full length cDNA of a putative eugenol synthase from tomato 'Micro-Tom' using rapid amplification of cDNA ends (RACE) technique and named a clone SlEGS. Open reading frame of SlEGS was 921bp long and its deduced amino acid sequence was 307bp. The BLAST analysis indicated that SlEGS shared high similarity with PhEGS1 (67.1%) and CbEGS2 (69.4%). Amino acid composition of SlEGS was determined by CLC genomics workbench tool and 3D structure of SlEGS was constructed by homology modeling using Swiss-PDB viewer and validated using PROCHECK and ProSA-web tool. In addition, the physiochemical properties of SlEGS was evaluated using ExPASy's ProtParam tool. Molecular weight was 33.93kDa and isoelectric point was 5.85 showing acidic nature. Other properties such as extinction coefficient, instability index, aliphatic index, and grand average hydropathy was also analyzed.

• Korean Journal of Remote Sensing
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• v.22 no.6
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• pp.469-483
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• 2006

An algorithm was developed to estimate stability indices (SI) over the Korean peninsula using Terra Moderate Resolution Imaging Spectroradiometer (MODIS) infrared brightness temperatures (TBs). The SI is defined as the stability of the atmosphere in the hydrostatic equilibrium with respect to the vertical displacements and is used as an index for the potential severe storm development. Using atmosphere temperature and moisture profiles from Regional Data Assimilation and Prediction System (RDAPS) as initial guess data for a nonlinear physical relaxation method, K index (KI), KO Index (KO), lifted index (LI), and maximum buoyancy (MB) were estimated. A fast radiative transfer model, RTTOV-7, is utilized for reducing the computational burden related to the physical relaxation method. The estimated TBs from the radiative transfer simulation are in good agreement with observed MODIS TBs. To test usefulness for the short-term forecast of severe storms, the algorithm is applied to the rapidly developed convective storms. Compared with the SIs from the RDAPS forecasts and NASA products, the MODIS SI obtained in this research predicts the instability better over the pre-convection areas. Thus, it is expected that the nowcasting and short-term forecast can be improved by utilizing the algorithms developed in this study.

• Journal of the Korean Geotechnical Society
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• v.22 no.11
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• pp.55-64
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• 2006

It is very Important to predict the damage zone of a rock mass induced by blasting for the excavation of an underground cavity such as a tunnel, as the damage zones incur mechanical and hydraulic instability of the rock mass potentially. Complicated blasting processes that can hinder the proper characterization of the damage zone can be effectively represented by two loading mechanisms. The first mechanism is the dynamic impulsive load-generating stress waves that radiate outwards immediately after detonation. This load creates a crushed annulus along with cracks around the blasthole. The second is the gas pressure that remains for an extended time after detonation. As the gas pressure reopens some arrested cracks and extends these, it contributes to the final structure of the damage zone induced by the blasting. This paper presents a simple method to evaluate the damage zone induced by gas pressure during rock blasting. The damage zone is characterized by analyzing crack propagations from the blasthole. To do this, a model of a blasthole with a number of radial cracks that are equal in length in a homogeneous infinite elastic plane is considered. In this model, crack propagation is simulated through the use of only two conditions: a crack propagation criterion and the mass conservation of the gas. The results show that the stress intensity factor of a crack decreases as the crack propagates from the blasthole, which determines the crack length. In addition, it was found that the blasthole pressure continues to decrease during crack propagation.

• Journal of Life Science
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• v.27 no.10
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• pp.1215-1224
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• 2017

Until now, various oncogenic pathways were idenfied. The accumulation of DNA mutation induces genomic instability in the cell, and it makes cancer. The development of bioinformatics and genomics, to find the precise and reliable biomarker is available. This biomarker could be applied the early-dignosis, prediction and convalescence of cancer. Recently, Transposable elements (TEs) have been attracted as the regulator of genes, because they occupy a half of human genome, and the cause of various diseases. TEs induce DNA mutation, as well as the regulation of gene expression, that makes to cancer development. So, we confirmed the relationship between TEs and colon cancer, and provided the clue for colon cancer biomarker. First, we confirmed long interspersed nuclear element-1 (LINE-1), Alu, and long terminal repeats (LTRs) and their relationship to colon cancer. Because these elements have large composition and enormous effect to the human genome. Interestingly, colon cancer specific patterns were detected, such as the hypomethylation of LINE-1, LINE-1 insertion in the APC gene, hypo- or hypermethylation of Alu, and isoform derived from LTR insertion. Moreover, hypomethylation of LINE-1 in proto-oncogene is used as the biomarker of colon cancer metastasis, and MLH1 mutation induced by Alu is detected in familial or hereditary colon cancer. The genes, effected by TEs, were analyzed their expression patterns by in silico analysis. Then, we provided tissue- and gender-specific expression patterns. This information can provide reliable cancer biomarker, and apply to prediction and diagnosis of colon cancer.