• Korean Journal of Microbiology
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• v.35 no.1
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• pp.7-12
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• 1999

By SDS-polyacrylamide gel elcctrophoresis (SDS-PAGE) and immunoblot analysis that a protein with 66,000 daltons in size was recognized by P. aeruginosa anti-exotoxin A from P. sp. PY002. The yields of exotoxin A in P. sp. PY002 culture were influenced by the concentration of iron in the culture media. Increasing of the exotoxin A production and siderophore production was made slight increasing in the MKB medium. On the other hand, to clone the gene encoding the exoloxin A genomic library of P. sp. PY002 was constructed in pBluescript SK(+). From this library a exotoxin A homologous gene was isolated by immunological hybridization method using P. aemginosa anti-exoloxin A as a probe. Two putative clones were isolated and designated pETA23 and pETA42. Thc restriction analysis ol pETA42 demonstrated that thc 1760 bp insert contained one NcoI, PvuII, SstI, Kpnl and EcoRI site and three SmaI and HaeD sites.

• Journal of Life Science
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• v.24 no.1
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• pp.1-7
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• 2014

Eukaryotic gene expression is an important process, which is initiated by several transcription factors and RNA polymerases that occupy the promoter region of genomic DNA. Although there are many experiments to identify the promoter region in a gene, it is time and labor consuming to finalize it. In this study, we utilized bioinformatic programs, including Ensembl, NCBI, and CpG plots, to identify the cloning promoter region in SETDB1 genomic DNA. We performed PCR amplification to obtain the SETDB1 promoter on an approximately 2 kb region upstream from the TSS named SETDB1-P1. The PCR product was ligated with TA cloning vectors, and we confirmed the insert size using restriction enzyme digestion. Sequentially, the insert was subcloned into a pGL3-luc vector to produce pGL3-SETDB1- P1-luc and then confirmed by DNA sequencing. We also obtained a fragmented PCR product called P2 and P3 and performed a luciferase assay using pGL3-SETDB1-P1-luc transfection. We found that several anticancer drugs, including taxol, 4-FU, and doxorubicin, decreased the promoter activity of SETDB1. We obtained consistent data on the regulation of SETDB1 gene expression after anticancer drug treatment using Western blot analysis and RT-PCR. Our results suggest that promoter cloning of the human SETDB1 gene utilizing bioinformatics is a very useful and timesaving approach to study gene expression.

• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.2
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• pp.304-310
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• 2006

Prevotella intermedia is one of the most frequently implicated pathogens in human periodontal disease and has a requirement for hemin for growth. This study has identified a hemin-binding P. intermedia protein by expression of a P. intermedia genomic library in Escherichia coli, a bacterium which does not require or transport exogenous hemin. The genomic library of P. intermedia was constructed into plasmid pUC18, transformed into Escherichia coli strain $DH5{\alpha}$, and screened for recombinant clones using heminbinding activity by plating onto hemin-containing agar. Approximately 5,000 recombinant E. coli colonies were screened onto LB-amp-hemin agar, single clone(pHem1) was exhibited a clearly pigmented phonotype. The 2.5kb insert DNA of pHem1 was determined by restriction enzyme mapping. Southern blot analysis of BamHI, BglII, EcoRI, HindIII and PstI-digested P. intermedia DNA indicated that single copy of the gene was present in the genome. Northern blot analysis revealed that the size of transcript was approximately 1.8 kb. The cloned gene contained a single ORF, consisting of approximately 850-residue amino acids. A BLAST search of the Institute for Genomic Research genes with similar nucleotide sequence revealed no significant similarity It needs further investigation to clarify the mechanisms of heme uptake in P. intermedia.

• The Journal of Korean Society for Radiation Therapy
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• v.10 no.1
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• pp.60-68
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• 1998

Treatment of a large diseased area with electron often requires the use of two or more adjoining fields. In such cases, not only electron beam divergence and lateral scattering but also fields overlapping and separation may lead to significant dose inhomogeneities(${\pm}20\%$) at the field junction area. In this study, we made Acrylic Electron Wedges to improve dose homogeneities(${\pm}5\%$) in these junction areas and considered application it to clinical practices. All measurements were made using 6, 9, 12, 16, 20MeV Electron beams from a linear accelerator for a $10{\times}10cm$ field at 100cm SSD. Adding a 1 mm sheet of acryl gradually from 1 mm to 15 mm, We acquired central axis depth dose beam profile and isodose curves in water phantom. As a result, for all energies, the practical range was reduced by approximately the same distance as the thickness of the acryl insert, e.g. a 1 mm thick acryl insert reduce the practical range by approximately 1 mm. For every mm thickness of acryl inserted, the beam energy was reduced by approximately 0.2MeV. These effects were almost independent of beam energy and field size. The use of Acrylic Electron Wedges produced a small increase $(less\;than\;3\%)\;in\;the\;surface\;dose\;and\;a\;small\;Increase(less\;than\;1\%)$ in X-ray contamination. For acryl inserts, thickness of 3 mm or greater, the penumbra width increased nearly linear for all energies and isodose curves near the beam edge were nearly parallel with the incident beam direction, and penumbra width was $35\;mm{\sim}40\;mm$. We decide heel thickness and angle of the wedge at this point. These data provide the information necessary to design Acrylic Electron Wedge which can be use to improve dose uniformity at electron field junctions and it will be effectively applicated in clinical practices.

• Journal of the Korean Society for information Management
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• v.35 no.4
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• pp.37-50
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• 2018

The purpose of this study is to analyze the first edition of Brown's Subject Classification and to understand the implications of today's library classification. For this purpose, the first edition of the Subject Classification published in 1906 was analyzed. The analysis results are divided into three main areas. First, SC is divided into eleven main classes and each class is subdivided into enumerated subdivisions from 000 to 999. Second, As a method of synthesizing the classification numbers, there were three methods of synthesis. There was a flexibility to insert a new classification number at the appropriate location when a new topic that does not appear in the main table appeared. Implications for classification studies can be divided into four main categories. First, SC proposed a method of classification number synthesis for composite topics, which is an innovative method that was not available in previous library classification. Second, the subject matter was explained in various aspects through the operation of auxiliary tables supporting the facets. Third, it is possible to easily insert a new topic into the classification system by using the SC that can have a flexible classification system for each library, or to use a short classification number according to the size of the library collection. Fourth, it provided a directory that can be considered as access points for digital materials.

• Journal of KIISE:Databases
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• v.33 no.6
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• pp.563-577
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• 2006

A stream processor uses resource sharing method for efficient of limited resource in multiple continuous queries. The previous methods process aggregate queries to consist the level structure. So insert operation needs to reconstruct cost of the level structure. Also a search operation needs to search cost of aggregation information in each size of sliding windows. Therefore this paper uses linear structure for optimization of sliding window aggregations. The method comprises of making decision, generation and deletion of panes in sequence. The decision phase determines optimum pane size for holding accurate aggregate information. The generation phase stores aggregate information of data per pane from stream buffer. At the deletion phase, panes are deleted that are no longer used. The proposed method uses resources less than the method where level structures were used as data structures as it uses linear data format. The input cost of aggregate information is saved by calculating only pane size of data though numerous stream data is arrived, and the search cost of aggregate information is also saved by linear searching though those sliding window size is different each other. In experiment, the proposed method has low usage of memory and the speed of query processing is increased.

• Journal of the Korean Ceramic Society
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• v.41 no.10 s.269
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• pp.728-734
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• 2004

The effect of microstructure of WC-Co substrates which have different WC grain sizes from submicron to 5 $\mu$m on the diamond-substrate adhesion strength was investigated. The substrates were pre-treated by two methods : chemical etching with Murakami's solution and subsequently with $H_2SO_4$, and thermal heat-treatment. The adhesion strength was estimated by degree of peeling after Rockwell indentation. Diamond films of 20 $\mu$m thickness deposited on the heat-treated substrates showed an excellent adhesion strength at the load of 100 kg, which ascribed to the large and elongated WC grains. However, the cutting edge of insert was deformed after heat treatment and the surface morphology of heat treated substrate strongly affected on the surface roughness of the deposited diamond films. On the contrary, the diamond film of 10 $\mu$m in thickness on the chemically etched substrates of average WC grain size over 2 $\mu$m showed good adhesion strength enough not to peel-off under a load of 60 kg. Especially, the substrate of average WC grain size over 5 $\mu$m exhibited much improved reliability of adhesion comparing with the substrate of average grain size under 2 $\mu$m. No substrate deformation was observed in this case after the chemical etching, which is more advantageous and more practical in terms of precious machining than the heat treatment case.

• Korean Journal of Optics and Photonics
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• v.19 no.2
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• pp.87-94
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• 2008

We present a new design for an endoscope objective lens composed of a lexible fiber bundle with 30,000 core, and a gradient-index (GRIN) objective lens with an optical adaptor. The characteristic of this objective lens is to be minimally-invasive to be able to insert easily in the internal organs of live animals. The GRIN lens has a small diameter and a very simple construction, which is selected with the diameter of 1.0 mm and numerical aperture of 0.5 to achieve a minimally-invasive outer diameter and a high resolution. The resultant designed lens shows the performance as follows; a lateral resolution of 1.63 um and diameters of 100% encircled energy of $0.3\;{\mu}m$ and $0.83\;{\mu}m$ for the on-axis and the off-axis image point, respectively. Also, we can present a cheap solution with a lateral resolution of 1.74 um and diameters of 100% encircled energy of $1.10\;{\mu}m$ and $2.84\;{\mu}m$ for the on-axis and the off-axis image point, respectively.

• Korean Journal of Veterinary Research
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• v.37 no.2
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• pp.269-280
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• 1997

The development and change of the cartilage canals ralated to chondrocyte growth and the major histological pattern were investigated in the calcaneus of the Korean native cattle fetuses from CRL 28mm to 175mm. The results as follows; 1. The cartilage canal first appeared in the caudal subperichondral region of the sustentaculum tali of the CRL 28mm fetus, and well developed after the period. 2. The three major cartilage canals, one entered from the head of calcaneous into where achilles tendon insert and the others from the caudal region of sustentaculum tali and the calcaneal sulcus, growth distinctively. The latters participated in the ossification center prior to the former. 3. The number of cells in this canals and the size increased synchronously with chondrocytic hypertrophy. In the intermediate and deep canals, the chondrogenic change showed distinctinvely. Our results demonstrated that the cartilage canals from the lower part were important the formation of the ossification center, and the growth and development of canals associated with the hypertropic changes of chondrocytes in the calcaneus of the Korean native cattle fetus.

• Fashion & Textile Research Journal
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• v.22 no.3
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• pp.368-377
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• 2020

This study provided basic data to develop functional and fitness cycling wear for cyclists. We analyzed the domestic and overseas cycling wear brand patterns for tops and tights as well as compared pads attached to tights. The results were as follows. The tops of the cycling wear were designed with cutting lines in the lateral or yoke in the back to reflect cycling posture. It is necessary to study if there is a difference in the air resistance or fitness of the athlete. The sleeve hem band affected by arm movement during cycling, proper sewing and band pressure are also important. The tights have a brand that does not insert a band in front of the waist in consideration of the cyclist's breathing. It should take into account methods of constructing a proper garment pressure that does not interfere with cycling. Analyzing the cycling pad indicated that the three-dimensional shape and size of the pad show many differences for each brand, indicating a difference in cycling movement suitability. In addition, many of the cutting lines should account for the productive aspects because of the high consumption of the fabric in order to design a pattern considering the cycling posture.