• Korean journal of applied entomology
• /
• v.56 no.4
• /
• pp.395-402
• /
• 2017

Overuse of synthetic pesticides caused negative side-effects such as environmental contamination, development of insect pests' resistance, and effects on non-target organisms. Plant origin substances without/or with low mammalian toxicity have been considered as promising alternatives to the synthetic pesticides. Fumigant toxicity of the essential oil of Iranian Yarrow, Achillea millefolium L., was investigated against a cosmopolitan stored-product insect pest: saw-toothed grain beetle (Oryzaephilus surinamensis L.). Chemical profile of this essential oil was studied by Gas Chromatography-Mass Spectrometry. Tested concentrations were significantly effective to the mortality of insect pest. A positive correlation between essential oil concentrations and pest mortality were realized. LC50 value (lethal concentration needed to 50% mortality) was achieved as $17.977(16.195{\pm}20.433){\mu}l/l$ air. The main components were 1,8-Cineole (13.17%), nerolidol (12.87%), ${\alpha}$-cubebene (12.35%), artemisia ketone (6.69%), ${\alpha}$-terpineol (5.27%), alloaromadendrene oxide (4.71%) and borneol (3.99%). Terpenic compounds including monoterpene hydrocarbons (8.19%), monoterpenoids (44.23%), sesquiterpene hydrocarbons (21.69%) and sesquiterpenoids (22.24%) were 96.35% of the total identified compounds. Results indicated that the terpene-rich A. millefolium essential oil may be considered as a safe bio-agent in the O. surinamensis management.

• Weed & Turfgrass Science
• /
• v.3 no.2
• /
• pp.114-120
• /
• 2014

Turfgrass insect pests were investigated in different turf sod production areas of Korea. Twelve insect pest species of 7 families in 6 orders and one Eriophyidae mite, Aceria zoysiae were collected from turf sod production areas. The zoysiagrass mite was most frequently occurred zoysiagrass sod production areas. Damaged rate by zoysiagrass mite was increased from May to September. Noctuidae (Spodoptera depravata, Agrotis ipsilon and A. segetum) and Pyralidae (Crambus sp.) insect pests in Lepidoptera were attracted in A. ipsilon sex pheromone trap. Eggs and larva of A. ipsilon was the highest occurred August. Zoysiagrass mite and A. ipsilon were main insect pests in turf sod production areas in Korea.

• Biomedical Science Letters
• /
• v.15 no.1
• /
• pp.37-45
• /
• 2009

Insect cell cultures have become important tools in the production of biological substances for use in a variety of research, human and veterinary medicine, and pest control applications. These applications often require the introduction of foreign DNA into the cells and have generally used methods originally developed for use with human and other mammalian cell cultures. While these methods can be successfully employed, they are often less efficient with insect cells and frequently involve complex procedures or require specialized equipment. Even when they do work, they may require substantial modification because of differences in the culture medium or growth patterns of insect cells. In this study, We have optimized transfection conditions of Sf9 cell line using insect expression vector pIZT/V5-His which expresses green fluorescent protein effectively. Human stem cell factor (hSCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. It also plays a key role in mast cell development, gametogenesis, and melanogenesis. It can exist in membrane-bound form and in proteolytically released soluble form. As determined by an enzyme-linked immunosorbent assay performed, hSCF level in supernatant averaged 995ng/ml. The human hSCF was partially purified by immunoaffinity chromatography and analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The results show that the hSCF has N-linked carbohydrate and corresponds to the soluble form, at or about 223 amino acids in length. The findings suggest functional importance for soluble hSCF in cells.

• Microbiology and Biotechnology Letters
• /
• v.27 no.5
• /
• pp.415-418
• /
• 1999

Thecodiplosis japonensis is an important insect pest in pine trees and hard to be controlled by chemical pesticides due to its characteristic life cycle. Experiments were conducted to determine the pathogenicity and growth temperature of several entomopathogenic fungi as potential microbial insectcides for T. japonensis. Beauveria bassiana SFB-SC and Paecilomyces fumosoroseus SFP-198 were highly pathogenic against T. japonensis. B. bassiana SFB-SC had also pathogenicity against a beneficial insect Bombyx mori. Growth rate and pathogenicity of P. funmosoroseus SFP-198 under 15$^{\circ}C$ was higher than those of B. bassians SFB-SC. These results indicated that P. fumosoroseus SFP-198 is a potential microbial control candidate for control of T. japonensis.

• Mycobiology
• /
• v.41 no.4
• /
• pp.245-247
• /
• 2013

The ambrosia beetle, Platypus koryoensis, is an economically important pest affecting oak trees in Korea. Candida kashinagacola was isolated from galleries of the beetle in oak wood and identified by analyses of morphology, physiological properties, and nucleotide sequence of the large subunit ribosomal DNA. This is the first report on Candida species associated with oak wilt disease vectored by the ambrosia beetle, Platypus koryoensis, in Korea.

• 한국약용작물학회:학술대회논문집
• /
• 2008.11a
• /
• pp.199-200
• /
• 2008

• 한국약용작물학회:학술대회논문집
• /
• 2008.11a
• /
• pp.197-198
• /
• 2008

• Proceedings of the Korean Society of Applied Entomolohy Conference
• /
• 2002.11a
• /
• pp.79-79
• /
• 2002

• Korean journal of applied entomology
• /
• v.42 no.1
• /
• pp.57-63
• /
• 2003

Accurate forecasting of occurrence time and abundance of insect pests is essential for developing technology of integrated pest management system. Digital image processing algorithms were utilized to automatically recognize rice planthoppers which are major insect pests in the rice cultivation field and were subsequently used to estimate densities in the field for efficient forecasting of insect pests. To the images taken in the rice field, image decomposition, top-hat transformation, threshold, and minimum and maximum filter were implemented for patterning individually the brown planthopper specimens attached at the bottom area of rice stems. In average 95.8cio of images were correctly recognized for estimating densities by the developed system, and the recognition rate was higher than that obtained from direct observations by experienced observers. Furthermore, the size of the recognized specimens was measured and was used for estimating the age structure in the observed brown planthopper populations.

• Journal of Species Research
• /
• v.12 no.2
• /
• pp.189-196
• /
• 2023

We monitored the hitchhiker insect pests from the international vessels entering into Korea in 2021. As a result, total of 581 individuals were detected by the survey based on visual inspection with naked eye. Among them, 500 individuals were identified as 244 species of 65 families under 11 orders through the integrative taxonomic method with DNA barcoding and morphological reexamination, but the remaining 81 individuals were classified as only to the family level. Of the 244 species identified, 26 species were determined to be not-distributed species in Korea (two Orthoptera, two Hemiptera, one Megaloptera, five Coleoptera, three Hymenoptera, and 13 Lepidoptera). Among them, two species, Sagra femorata (Chrysomelidae, Coleoptera) and Dendrolimus punctatus (Lasiocampidae, Lepidoptera), were discovered as 'Regulated species' listed by Animal and Plant Quarantine Agency, South Korea. Therefore, we reported on the 26 not-distributed species in Korea and provided inanimate pathway information such as navigation routes on the vessels hitchhiking the species, state of the samples at the time of detection, identification results and original distribution for the detailed monitoring and the risk analysis on the species.