• Title/Summary/Keyword: Insect pest

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Antimicrobial Active Substances from Entomopathogenic Fungi (Various Applications of Entomopathogenic Fungi)

  • Shin, Tae Young;Woo, Soo Dong;Kim, Jeong Jun
    • 한국균학회소식:학술대회논문집
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    • 2016.05a
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    • pp.13-13
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    • 2016
  • Insects constitute the largest and most diverse group of animals in the world. They also serve as the hosts or nutrient sources for an immense assemblage of pathogens, parasites, and predators. More than 700 fungal species from 100 genera have adopted an entomopathogenic lifestyle. Although entomopathogenic fungi were studied as only biocontrol agents against a variety of pests in various countries, it has been recently focused their additional roles in nature. They are antagonists to/against plant pathogens, endophytes, and possibly even plant growth promoting agents. The potential antimicrobial effect against fungal plant pathogens by an isolate of entomopathogenic fungi including Beauveria bassiana, Lecanicillium spp., and Isaria fumosorosea have been reported since late 1990s, but wasn't reported pathogenicity of the isolate against pests. Later, a Canadian Lecanicillium sp. isolate and L. longisporium isolated from Vertalec$^{(R)}$ showed simultaneous control effect against both aphid and cucumber powder mildew. Therefore, the antimicrobial activities of 342 fungi isolates collected from various regions and conditions in Korea were evaluated against plant pathogenic fungus Botrytis cinerea using dual culture technique on agar plate. As a result, 186 isolates (54.4%) shown the antifungal activity against B. cinerea. The culture filtrates of selected fungi completely suppressed the growth of the microorganisms, indicating that suppression was due to the presence of antimicrobial substances in the culture filtrate. Mode of action of these fungi against insect involves the attachment of conidia to the insect cuticle, followed by germination, cuticle penetration, and internal dissemination throughout the insect. During infection process, secreted enzymes, proteinous toxins, and/or secondary metabolites secreted by entomopathogenic fungi can be used to overcome the host immune system, modify host behavior, and defend host resources. Recently, secondary metabolites isolated from entomopathogenic fungi have been reported as potential bioactive substances. Generally, most of bioactive substances produced by entomopathogenic fungi have reported low molecular weight (lower than 1,000 g/mol) as peptide and, in contrast the high molecular weight fungal bioactive substances are rare. Most substances based on entomopathogenic fungi were shown antimicrobial activity with narrow control ranges. In our study we analyzed the antimicrobial substances having antagonistic effects to B. cinerea. Antimicrobial substances in our fungal culture filtrates showed high thermostability, high stability to proteolytic enzymes, and hydrophilicity and their molecular weights were differed from substance. In conclusion, entomopathogenic fungi showed pathogenicity against insect pests and culture filtrate of the fungi also shown to antimicrobial activity. In the future, we can use the entomopathogenic fungi and its secondary metabolites to control both insect pest control and plant pathogenic fungi simultaneously.

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Report on the Hitchhiker Insect Pests detected from the Foreign Vessels Entering into Korea II (국외에서 출항한 국내 입항 선박에서 검출된 한국 미분포 편승자 해충에 대한 보고 II)

  • Kang, Tae Hwa;Kim, Sang Woong;Hong, Ki-Jeong;Kim, Nam Hee;Cho, Il Kyu;Choi, Deuk-Soo
    • Korean journal of applied entomology
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    • v.60 no.3
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    • pp.269-276
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    • 2021
  • During 15 months from 13. Jul. 2018 to 29. Oct. 2019, we monitored on the hitchhiker insect pests from a total of 306 foreign vessels entering into Korea. As the results, a total of 805 samples of the hitchhiker insects were collected using simply collecting method by hands and identified as 379 species of 78 families under 12 orders on the basis of integrative species identification. Among them, 42 species (67 individuals) of 21 families under seven orders were detected as not-distributed species in South Korea. Of the 42 not-distributed species, seven species were multiply detected in 2018 and 2019, and two species, Erthesina fullo (Pentatomidae, Hemiptera) and Tessaratoma papilosa (Tessaratomidae, Hemiptera) were discovered as regulated insect pest. Therefore, we reported on the 42 not-distributed species in South Korea with the data sheets for the establishment of a monitoring plan on the not-distributed species and the risk assessments on invasive or invasive likelihood species.

Studies on Insect Diversity Related to Genetically Engineered Vitamin A Rice under Large Scale Production (비타민 A 강화 벼의 대규모 GMO 포장에서 곤충다양성 분석)

  • Kim, Kyung-Min;Ryu, Tae-Hun;Suh, Sang Jae
    • Korean Journal of Breeding Science
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    • v.42 no.2
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    • pp.157-162
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    • 2010
  • This study was carried out to develop of macro-protocol and the biosafety guide for 'Nakdong', and 'Golden Rice' (genetically modified vitamin A rice) by large scale field trial of GM crops. Typically, when a new GM crop is created, breeders should conduct field test to make sure the GM crop is safe, and provide some information on GM crops for approval. A total of isolated 4,700 $m^2$ field for trial of GM crops were prepared, and 'Nakdong' and 'Golden rice' were cultivated by standard method of RDA (Rural Development Administration, Korea). Field studies indicated that the population densities of insect pests and natural enemies have no difference between two varieties. While insect pest density on 'Nakdong' was slightly higher than on Golden Rice, but natural enemy density on Golden Rice was a little higher. These results provided the insect diversity for risk assessment analysis of Golden Rice and suggested that the macro-protocol could be useful to detect GM plants.

Isolation and indentification of soil bacteria for biocontrol the Cockroach(Blattella germanica L.) (바퀴(Blattella germanica L.)의 생물학적 제어를 위한 토양세균의 분리 및 동정)

  • 이광배;몽룡곤
    • Journal of environmental and Sanitary engineering
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    • v.8 no.1
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    • pp.129-138
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    • 1993
  • The marked spread of the cockraches of recent years has raised a great social problem in urban areas. The cockroach have to remove1 because transmit a disease to human as pest insect, but particulars are not yet reported on biological control agent for the cockroach removal. This study was tried for the first time on biological control for the cockroach removal. The obtained results were as follows : 1. The isolated were spore-forming bacillus 1098 strain in soil. The No. 109(TH 109) strain of the among spore-forming bacillus was showed the poisonous against Cockroach. 2. The biological characteristics and flagella antigenicity of the strain is similar to Bacillus thur-ingiensis subsp. indiana. 3. TH 109 strain have the delta-endotoxin of cuboid shap. 4. This delta-endotoxin of product by TH 109 strain was toxic to the cockroach(Blattella gemzanica. L).

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Ecological Studies on the Cigarette Beetle, Lasioderma serricorne F. (Coleoptera: Anobiidae) Parasitic Hymenoptera, Lariophagus ditinguendus F., (Hymenoptera: pteromalidae) in Tobacco Storage Warehouse (원료담배 저장창고에서의 궐련벌레, Lasioderma serricorne F.(Coleoptera: Anobiidae),에 기생하는 바구미살이금좀벌, Lariophagus distinguendus F. (Hymenoptera : Pteromalidae),의 발생 생태에 관한 연구)

  • 오명희
    • Journal of the Korean Society of Tobacco Science
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    • v.21 no.2
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    • pp.188-194
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    • 1999
  • Cigarette beetle, Lasioderma serricorne F.(Coleoptera : Anobiidae), is one of the most serious insect pest of stored leaf tobacco in Korea. The first adults of their ecto-parasitic hymenoptera, Lariophagus ditinguendus F. (Hymenoptera: Pteromalidae), were emerged in late June in 1992, in early May in 1993 and 1995, in late May in 1994 and 1996. This parasitic hymenoptera had two peak times of adults emergence. The first one was from late June to early July and the 2nd peak time in the Suwon area occurred from late August to early September in 1993, 1994 and 1996, and late September in 1992 and 1995.

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Screening of Insecticides for Control of Spodoptera exigua in Double Cropping after Early Rice Alisma plantago

  • Kwon, Byung-Sun;Shin, Jong-Sup;Park, Hee-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.5
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    • pp.345-347
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    • 2001
  • This study was conducted to evaluate the effect of insecticides on control of Spodoptera exigua, growth characteristics, and dry root yield in the cultivation of Alisma plantago after early maturing rice cropping. All pesticides treated had no effect on growth and flowering rate of Alisma plantago. The major insecticides were teflubenzuron Wp, 5%, tebufenozide Wp, 8%, iufenuron Ec, 5%, and tebufenozide Wp, 5%. Dry root yield were increased largely with tebufenozide Wp, 5%(20 g/20 l), insecticide than the other insecticides and no control. All insecticides had no injury with standard dosage. On the other hand, all insecticides had slight injury in the double dosage level for the Alisma plantago.

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Survey of Farmer Informationization State and Needs for Knowledge based Agricultural Information System (지식기반 농업정보시스템 구축을 위한 농민 정보화 실태 및 지식수요 조사)

  • Kim, Hong-Yeon;Jung, Nam-Su;Jang, Woo-Suk;Oh, Tae-Suk;Lim, Chang-Su
    • Journal of Korean Society of Rural Planning
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    • v.16 no.4
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    • pp.139-145
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    • 2010
  • Agricultural information was discussed for suggesting tasks and solutions of knowledge based information system. States of agricultural information systems in rural development administration were described and problems and tasks were summarized in knowledge needs survey of farmers who have to decide many alternatives for farming. Hard to access information are soil state, disease and insect pest. Important information in agriculture are water, soil fertility, soil physical property, and accessibility from main road. In conclusion, knowledge based agricultural information system can be developed based on surveyed needs.

Screening of Insecticide to Control Rhophalosiphum nymphaeae for Alisma plantago as Second Crop in Paddy Field

  • Kwon, Byung-Sun;Lim, June-Taeg;Shin, Jeong-Sik
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.1
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    • pp.17-19
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    • 2003
  • The purpose of this study was to evaluate the effect of pesticides on insect pest control of Rhophalosiphum nymphaeae, growth characteristies and dry root yield from the cultivated after early maturing rice cropping. All pesticides treated had no effect on the growh period, flowering rate of Alisma plantago. The major pesticides were methomyl-Lf, 24.1 %, imidacloprid-Wp, 10%, carbosulfan-Wp, 20%, and methomyl-Wp, 45%. Dry yield of root were increased largely with imidacloprid-Wp, 10% (10g/20$\ell$), pesticide than the other pesticides and control. All pesticides were had no injury with recommended concentration. On the other hand all pesticides were slightly harmful in the double dosage level for the Alisma plantago.

Biodeterioration of Cultural Property and Fumigation (문화재의 생물열화 방제-훈증처리를 중심으로)

  • Lee, Ho-Bong
    • 보존과학연구
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    • s.13
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    • pp.69-80
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    • 1992
  • A great numbers of cultural properties destroyed though attack by insect pests and microorganisms. Biodeterioration damage is particularly serious in this country because many cultural propertiese are made of organic materials. Recently, there are various countermeasures of biodeterioration or alternative methods are reported, such as Gamma Radiation, Micro-wave Irridation, Freezing, Inert Atmosphere (Oxygen-less atmosphere), and Environmental Control. However its practical application are limited and some difficulties for treatment of large objects. Fumigation is one of the most useful and effective methods of control biodeterioration because it gives less damage of cultural properties but rapidly eradicate infesting organisms at one action. This paper evaluated selected fumigants and fumigation methods with emphasis on the following paragraph:1) Effectiveness of selected fumigants on insects and microbes involved inbiodeterioration.2) Physical and chemical characters of selected fumigants.3) Less toxic new alternative fumigant and its mixtures.4) Inert atmosphere (Oxygen-less atmosphere)5) Methods of fumigation : Sealed fumigation, Covered fumigation and Vacuum fumigation (Reduced-pressure fumigation)

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