• 제목/요약/키워드: Insect pest

검색결과 421건 처리시간 0.041초

머리대장가는납작벌레의 합리적 방제 물질로 이란 서양가새풀 정유의 살충효과 평가 (Essential Oil Isolated from Iranian Yarrow as a Bio-rational Agent to the Management of Saw-toothed Grain Beetle, Oryzaephilus surinamensis (L.))

  • 아스가르 에바돌라히
    • 한국응용곤충학회지
    • /
    • 제56권4호
    • /
    • pp.395-402
    • /
    • 2017
  • 유기합성 농약의 과다사용은 환경오염, 살충제 저항성 발달, 비표적 생물에 대한 영향 등 부작용의 원인이 되고 있다. 유기합성 농약의 대체약제로 포유동물에 저독성인 식물기원 물질이 각광을 받게 되었다. 이란 서양가새풀(Achillea millefolium L.) 정유성분의 훈증독성은 국제적 저장작물 해충인 머리대장가는납작벌레(Oryzaephilus surinamensis L.)의 방제제로 연구된 바 있다. 이 식물 정유의 화학적 성분을 가스크로마트 그래피(MS)를 이용하여 분석하였다. 살충실험 결과 처리농도에 따라 유의한 살충률을 나타냈다. 처리농도와 살충률 간 양의 상관관계가 있었다. 반치사 농도(LC50)는 $17.977{\mu}l/L$ 이었다. 주요 성분은 1,8-Cineole (13.17%), nerolidol (12.87%), ${\alpha}$-cubebene (12.35%), artemisia ketone (6.69%), ${\alpha}$-terpineol (5.27%), alloaromadendrene oxide (4.71%) 및 borneol (3.99%) 이었다. 전체 동정된 화합물의 96.35%는 Terpenic 화합물로 monoterpene hydrocarbons (8.19%), monoterpenoids (44.23%), sesquiterpene hydrocarbons (21.69%) 및 sesquiterpenoids (22.24%)를 포함하고 있었다. 본 결과는 terpene이 풍부한 서양가새풀 정유가 머리대장가는납작벌레의 안전한 생물농약으로 고려될 수 있음을 보여주었다.

잔디 재배지 발생 해충 종류 (Insect Pests in Turf Sod Production Areas in Korea)

  • 이채민;권오경;이광수;이상명;최성환;이동운
    • Weed & Turfgrass Science
    • /
    • 제3권2호
    • /
    • pp.114-120
    • /
    • 2014
  • 우리나라 잔디재배지역에 발생하는 잔디 해충을 알아보기 위하여 조사를 수행하였다. 6목 7과 12종의 해충과 한 종의 Eriophyidae 응애류인 잔디혹응애(Aceria zoysiae)가 잔디 재배지에서 채집되었다. 잔디혹응애는 한국잔디류(Zoysia spp.) 재배지에서 가장 빈번하게 발생하였고, 피해율은 5월에서 9월로 갈수록 증가하였다. 나비목 밤나방과 해충인 잔디밤나방(Spodoptera depravata)과 검거세미나방(Agrotis ipsilon), 거세미나방(A. segetum), 포충나방과 해충인 포충나방류(Crambus sp.)가 검거세미나방 성페로몬트랩에 유인되었다. 검거세미나방 알과 유충은 8월에 가장 많이 발생하였다. 잔디혹응애와 잔디밤나방이 우리나라 잔디 재배지에서 가장 중요한 해충이었다.

Production of Recombinant Protein, Human Stem Cell Factor, Using Insect Cell Line

  • ;;;;;;;권오유
    • 대한의생명과학회지
    • /
    • 제15권1호
    • /
    • pp.37-45
    • /
    • 2009
  • Insect cell cultures have become important tools in the production of biological substances for use in a variety of research, human and veterinary medicine, and pest control applications. These applications often require the introduction of foreign DNA into the cells and have generally used methods originally developed for use with human and other mammalian cell cultures. While these methods can be successfully employed, they are often less efficient with insect cells and frequently involve complex procedures or require specialized equipment. Even when they do work, they may require substantial modification because of differences in the culture medium or growth patterns of insect cells. In this study, We have optimized transfection conditions of Sf9 cell line using insect expression vector pIZT/V5-His which expresses green fluorescent protein effectively. Human stem cell factor (hSCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. It also plays a key role in mast cell development, gametogenesis, and melanogenesis. It can exist in membrane-bound form and in proteolytically released soluble form. As determined by an enzyme-linked immunosorbent assay performed, hSCF level in supernatant averaged 995ng/ml. The human hSCF was partially purified by immunoaffinity chromatography and analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The results show that the hSCF has N-linked carbohydrate and corresponds to the soluble form, at or about 223 amino acids in length. The findings suggest functional importance for soluble hSCF in cells.

  • PDF

솔잎혹파리의 병원성 진균 Beauveria bassiana 및 Paecilomyces fumosoroseus의 온도별 병원성과 생장 변화 (Variations in Growth and Pathogenicity of Beauveria bassiana and Paecilomyces fumosoroseus Pathogenic to the Pine Gall Midge, Thecodiplosis japonensis)

  • 이인기;심희진;우수동;제연호;양자문;강석권
    • 한국미생물·생명공학회지
    • /
    • 제27권5호
    • /
    • pp.415-418
    • /
    • 1999
  • Thecodiplosis japonensis is an important insect pest in pine trees and hard to be controlled by chemical pesticides due to its characteristic life cycle. Experiments were conducted to determine the pathogenicity and growth temperature of several entomopathogenic fungi as potential microbial insectcides for T. japonensis. Beauveria bassiana SFB-SC and Paecilomyces fumosoroseus SFP-198 were highly pathogenic against T. japonensis. B. bassiana SFB-SC had also pathogenicity against a beneficial insect Bombyx mori. Growth rate and pathogenicity of P. funmosoroseus SFP-198 under 15$^{\circ}C$ was higher than those of B. bassians SFB-SC. These results indicated that P. fumosoroseus SFP-198 is a potential microbial control candidate for control of T. japonensis.

  • PDF

A New Record of Candida kashinagacola (Synonym Ambrosiozyma kashinagacola) from Galleries of Platypus koryoensis, the Oak Wilt Disease Vector, in Korea

  • Suh, Dong Yeon;Kim, Seong Hwan;Son, Seung Yeol;Seo, Sang Tae;Kim, Kyung Hee
    • Mycobiology
    • /
    • 제41권4호
    • /
    • pp.245-247
    • /
    • 2013
  • The ambrosia beetle, Platypus koryoensis, is an economically important pest affecting oak trees in Korea. Candida kashinagacola was isolated from galleries of the beetle in oak wood and identified by analyses of morphology, physiological properties, and nucleotide sequence of the large subunit ribosomal DNA. This is the first report on Candida species associated with oak wilt disease vectored by the ambrosia beetle, Platypus koryoensis, in Korea.

디지털 영상처리 알고리즘을 이용한 벼멸구류의 밀도측정 (Density Estimation of Rice Planthoppers Using Digital Image Processing Algorithm)

  • 박영석;김황용;엄기백;박창규;이장명;전태수
    • 한국응용곤충학회지
    • /
    • 제42권1호
    • /
    • pp.57-63
    • /
    • 2003
  • 해충의 발생시기와 발생량에 대한 정확한 예찰정보는 해충의 효율적인 종합적 방제를 위하여 필수적으로 요구된다. 해충의 효율적인 발생 예찰조사를 위해 디지털 영상처리 알고리즘을 이용하여 벼농경지에서 주요 해충인 멸구류를 자동적으로 인식하고 밀도를 측정하도록 하였다. 야외경작지에서 촬영한 입력영상에 대해 구성인자분해과정, 탑헷(top-hat)변환, 역치적용, 최소/최대 필터링 등의 방법을 적용하여 벼 잎에 붙어 있는 멸구 개체를 인식하고 개체수를 헤아렸다. 평균인식율은 95.8%를 보였다. 또한 인지된 각 멸구류 개체 크기를 측정하여 멸구류의 연령분포 추정을 가능하게 하였다

Alien hitchhiker insect species detected from the international vessels entering into Korea in 2021

  • Tae Hwa Kang;Nam Hee Kim;Sang Woong Kim;Deuk-Soo Choi
    • Journal of Species Research
    • /
    • 제12권2호
    • /
    • pp.189-196
    • /
    • 2023
  • We monitored the hitchhiker insect pests from the international vessels entering into Korea in 2021. As a result, total of 581 individuals were detected by the survey based on visual inspection with naked eye. Among them, 500 individuals were identified as 244 species of 65 families under 11 orders through the integrative taxonomic method with DNA barcoding and morphological reexamination, but the remaining 81 individuals were classified as only to the family level. Of the 244 species identified, 26 species were determined to be not-distributed species in Korea (two Orthoptera, two Hemiptera, one Megaloptera, five Coleoptera, three Hymenoptera, and 13 Lepidoptera). Among them, two species, Sagra femorata (Chrysomelidae, Coleoptera) and Dendrolimus punctatus (Lasiocampidae, Lepidoptera), were discovered as 'Regulated species' listed by Animal and Plant Quarantine Agency, South Korea. Therefore, we reported on the 26 not-distributed species in Korea and provided inanimate pathway information such as navigation routes on the vessels hitchhiking the species, state of the samples at the time of detection, identification results and original distribution for the detailed monitoring and the risk analysis on the species.