• Molecules and Cells
• /
• v.28 no.3
• /
• pp.155-165
• /
• 2009

The newly sequenced complete mitochondrial genome of the brown marmorated stink bug, Halyomorpha halys($St{\aa}l$) (Hemiptera: Pentatomidae), is a circular molecule of 16,518 bp with a total A+T content of 76.4% and two extensive repeat regions in A+T rich region. Nucleotide composition and codon usage of H. halys are about average when compared with values observed in 19 other published hemipteran mitochondrial genomes. Phylogenetic analyses using these 20 hemipteran mitochondrial genomes support the currently accepted hypothesis that suborders Heteroptera and Auchenorrhyncha form a monophyletic group. The mitochondrial gene arrangements of the 20 genomes are also consistent with our results.

• Korean journal of applied entomology
• /
• v.61 no.1
• /
• pp.63-75
• /
• 2022

Semiochemicals including pheromone are chemicals used in chemical communication of insect. Semiochemicals have been widely used for population monitoring, mass trapping, and mating disruption of insect pest. In this review article, the current status of pheromone research of major forest insect pest in Korea such as Monochamus alternauts, M. saltuarius, Matsucoccus thunbergianae, Platypus koryoensis, Glyphodes perspectalis, Dioryctria abietella, Lymantria dispar, Synanthedon bicingulata, and Naxa seriaria was introduced, and the results were compared with those reported in other countries. Based on the analysis of current pheromone research of forest insect pests, future studies and development direction was suggested.

• The Korean Journal of Food And Nutrition
• /
• v.22 no.4
• /
• pp.492-496
• /
• 2009

The composition of the bacterial populations in Gorosoe(Acer mono Max.) sap was characterized during storage with different heat treatments($63^{\circ}C$ for 30 min and $73^{\circ}C$ for 15 sec). The saps were aseptically collected at 0, 15 and 30 days of storage and analyzed by dilution plating and 16S rDNA PCR-DGGE analysis. There were significant differences in the total number of colony forming units(CFUs) of bacteria between heated and nonheated saps. Bacteria of nonheated sap were present at a level of $3.4{\times}10^7CFU/m{\ell}^{-1}$, whereas living bacteria were not detected in the heated sap. According to the 16S rDNA sequence and DGGE analysis, Pseudomonas sp. was the most abundant bacterial strain in the samlpes, and the bacterial community structures become more simplified with time and were composed of the Chryseobacterium sp. with time. These results allowed us to characterize the dominant bacteria involved in Gorosoe sap and to better understand their dynamics throughout storage.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.12
• /
• pp.1644-1653
• /
• 2014

Wuyiencin is produced by Streptomyces ahygroscopicus var. wuyiensis CK-15 and is widely used as an antifungal agent in agriculture. Analysis of wuyiencin biosynthetic gene clusters reveals wysR, a member of the LAL-family of transcriptional regulatory genes. WysR consists of an N-terminal PAS domain and a LuxR family C-terminal helix-turn-helix motif. However, the roles of wysR in wuyiencin biosynthesis are largely unknown. In this study, we showed that inactivation of wysR resulted in the complete loss of wuyiencin production, which could be restored by complementation with a single copy of wysR. Furthermore, we successfully increased wuyiencin production to a significantly higher level by overexpression of wysR in S. wuyiensis CK-15. Quantitative real-time RT-PCR analysis showed that WysR regulates wuyiencin biosynthesis by modulating other putative regulatory genes. Thus, WysR was identified as an activator of wuyiencin biosynthesis, and overexpression of wysR gene proved to be an effective strategy for improving wuyiencin production.

• Korean journal of applied entomology
• /
• v.56 no.1
• /
• pp.41-49
• /
• 2017

The community of xylophagous beetles belonging to Cerambycidae, Curculionidae and Scolytinae in Korean white pine, Pinus koraiensis Siebold & Zuccarini, forests was surveyed using Malaise traps in 2007. A total of 1,615 xylophagous beetles were collected, including 184 cerambycids from 15 species, 185 curculionids from 17 species, and 1,246 scolytid beetles from 6 species, of which the dominant species was the ambrosia beetle Xyleborus mutilatus Blandford. Ranked by order of population size, the wood-boring and bark beetle community in Korean white pine showed high dominance by one species of Scolytinae, suggesting the community had low biological diversity. Thinning in Korean white pine forests influenced on the abundance of bark and ambrosia beetles, whose populations in particular stands increased 1 year after thinning, and then decreased the following year.

• The Korean Journal of Mycology
• /
• v.26 no.4 s.87
• /
• pp.413-415
• /
• 1998

An entomophthoraceous fungus, Zoophthora phalloides, was found in populations of the aphid Dactynotus species, at National Institute of Agricultural Science and Technology, and Jeonnam Provincial RDA during June, 1998. Occurrence of the fungus and aphid has never been recorded in Korea. Microscopic observations of this fungus are described, and illustrated. The fungus has sausage-shaped primary conidia with $28.98\;{\mu}m$ in length, and two types of secondary conidiophores. Our specimens exhibit closer relationship with the North American isolates than with the European isolates on the basis of conidial length described by other study.

• Korean journal of applied entomology
• /
• v.48 no.4
• /
• pp.447-451
• /
• 2009

Locust gall midge (LGM), Obolodiplosis robiniae (Haldeman) (Diptera: Cecidomyiidae), is a cecidomyiid insect forming roll-up galls on leaves of Robinia pseudoacacia Linnaeus (Fabaceae). LGM, known as native to North America, was reported from Korea and Japan in 2002. LGM was observed weekly or biweekly to clarify their voltinism and distribution within the crown of the host tree in two sites of Osan and Siheung in Korea from May to August, 2007. Density of LGM was investigated based on the number of larvae per leaf. Two generations of LGM were observed in Siheung site whereas three generations in Osan site during the present study. The result indicated that LGM had at maximum three generations per year. The density of LGM in Osan was higher in the upper crown of the host trees than middle or lower part. In Siheung, LGMs were distributed more on the exterior of the lower crown than the interior. The average number of larvae per gall was $3.3{\pm}0.1$ and $2.8{\pm}0.1$ individuals per leaf in Osan and Siheung, respectively.

• Proceedings of the Korean Society of Organic Agriculture Conference
• /
• 2009.12a
• /
• pp.45-56
• /
• 2009

Current status on the occurrence and the management of the major disease, insect and mite pests were investigated in the organic or non-chemical pest control orchards from 2005 to 2009. Numbers of certified organic or non-chemical apple orchards were increased from 14 in 2005 to 78 in 2008. Severe damages on leaves and fruits occurred by the several diseases such as marssonina blotch, bitter rot, white rot, sooty blotch and flyspeck, and the several insect pests such as apple leaf-curling aphid, woolly apple aphid, oriental fruit moth and peach fruit moth on the almost certified organic or non-chemical pest control orchards. About 10 and 18 environmental-friendly materials were used to control diseases and insect or mite pests respectively. But, lime sulfur and bordeaux mixture to diseases and machine oil, plant oil mixed with egg yolk, and pheromone mating disruptions to insect pests were effective to control under the adequate conditions. At present, it is extremely difficult to produce organic apples in Korea. Growers must consider about and solve so many conditions on the cultivar, weather, local site, marketing and so on, before when they decide to change from conventional or IPM(Integrated Pest Management) to organic or non-chemical pest control orchards.

• Korean Journal of Organic Agriculture
• /
• v.18 no.2
• /
• pp.221-232
• /
• 2010

During 2005~2009, current status on the occurrence and the management of the major disease, insect and mite pests were investigated in the non-chemical or organic cultured apple orchards in Korea. Numbers of certified organic or non-chemical apple orchards increased from 14 in 2005 to 78 in 2008. Severe damages on leaves and fruits were caused by the several diseases such as marssonina blotch, bitter rot, white rot, sooty blotch and flyspeck, and the several insect pests such as apple leaf-curling aphid, woolly apple aphid, oriental fruit moth and peach fruit moth on the almost certified organic or non-chemical pest control orchards. About 10 and 18 environmental-friendly materials were used to control diseases and insect or mite pests, respectively. But, lime sulfur and bordeaux mixture to diseases and machine oil, plant oil mixed with egg yolk, and pheromone mating disruptions to insect pests were effective under the adequate conditions.

• Weed & Turfgrass Science
• /
• v.6 no.2
• /
• pp.77-85
• /
• 2017

Insect pests have been one of the main constraints affecting turf sod cultivation areas, playgrounds, parks, golf courses, airports and other related recreational grounds. However, turfgrass research has been very limited and confined within a limited period of time compared to other branches of crop science in Korea. This review was surveyed from all papers in KCI (Korea Citation Index), SCI (Scientific Citation Index) and SCIE (SCI Expended) journals related to turfgrass research in Korea. Fifty two papers concerning turfgrass insect pest in Korea have hitherto been published in 8 different scientific journals since 1990. Thirty three papers (63.5% of total) were published in Weed and Turfgrass Science. The main topics of focus were divided into two parts; ecological study and control of insect pests. Before the 1990 decade, there were no scientific papers published in relation to turfgrass insect pest science. However, during the 1990, 2000 and 2010 decades, 9, 10 and 14 papers were published respectively in Weed and Turfgrass Science. From 1997, about 2 papers were published per year in the scientific journals. Thirty three insect pest species belonging to 13 families in 6 orders, one Eriophyidae mite, Aceria zoysia and one Armadillidae sowbug, Armadillidium vulgare, have hitherto been listed as turfgrass arthropod pests in Korea. Fifty percent of turfgrass insect pest papers published were focused on whitegrub, Adoretus tenuimaculatus, Popillia quadrigutta and earthworm research in Korea.