• 한국토양비료학회지
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• 제45권5호
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• pp.817-821
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• 2012

Soil samples collected from abounded mines of Boryeong area in South Korea were used in isolating bacterial strains and their capacity to solubilize inorganic phosphates and heavy metal tolerance were assessed in vitro. Three different inorganic phosphate sources (Ca phosphate, Fe phosphate, and Al phosphate) and four different heavy metals (Co, Cd, Pb and Zn) each with three concentrations ($100{\mu}g\;mL^{-1}$, $200{\mu}g\;mL^{-1}$, and $400{\mu}g\;mL^{-1}$) were used. The bacterial isolates PSB-1, PSB-2, PSB-3, and PSB-4 solubilized significantly higher amount of Ca phosphate during the first five days of incubation though subsequent drop in soluble phosphorus level in the medium was observed at the later stage (after 5 days) of the incubation. Solubilization of Ca phosphate and Fe phosphate was concomitant with the acidification of the culture medium compared to the control where it remained constant. Isolated strains could solubilize Fe phosphate to certain extent ($25-45{\mu}g\;mL^{-1}$) though solubilization of Al phosphate was found negligible. All the isolates were tolerant to heavy metals (Cd, Pb, and Zn) up to the concentration of $400{\mu}g\;mL^{-1}$ except PSB-1 and PSB-8, which were shown to be vulnerable to Co even at $100{\mu}g\;mL^{-1}$. Heavy metal tolerant strains should be further evaluated for plant growth promoting activities also under field conditions in order to assess their agricultural and environmental significance.

• 한국건축시공학회지
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• 제19권4호
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• pp.289-297
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• 2019

현재 건축물에 사용되는 접착제는 주로 유기질 재료인 에폭시 수지 접착제가 사용되고 있다. 유기질 재료인 에폭시 수지 접착제는 유기성 물질로 접착제 생산 시 유해물질이 발생하며, 시공 후에도 유해물질이 실내거주공간에서 높게 나타난다. 또한 건축물 화재 시 유기 재료에서 발생되는 다량의 일산화탄소가 인명피해로 이어진다. 본 연구는 순수무기계 재료인 산화마그네시아, 인산염, 붕사를 사용한 무기 접착재에 대해 기존 유기 접착제를 대체 할 무기 접착재로의 활용가능성을 평가한 연구이다. 적정 인산염 선정에 관한 실험과 지연제로서 활용한 붕사의 첨가율에 대한 특성을 실험한 결과 적정 인산염으로 제1인산칼륨이 도출되었으며, 붕사 첨가율에 따른 특성을 측정한 결과 KS F 4923의 품질기준과 비교하여 경화수축률과 가열변화율은 품질기준을 만족하였으며, 인장강도는 붕사 첨가율 4% 이상에서 만족하는 결과가 나왔지만 접착강도의 경우 품질 기준을 충족하지 못하였다. 향후 접착강도 증진을 위한 추가적인 연구가 필요할 것으로 판단된다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.403-406
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• 2002

This experimental shows the possibility of using as biofertilizer, which convert insoluble inorganic phosphate salts to plant-usable phosphate type by immobilized microorganism with calcium alginate. In the case of culture of P. agglomerans on constant medium pH, phosphate was produced 357 mg/L after 18hrs. And in the case of culture of immobilzed P. agglomerans bead, phosphate was produced maximum 295.6 mg/L after 120 hrs. Also as using rock phosphate as insoluble phosphate salts, phosphate was respectably produced 190.3 and 195.2 mg/L after 36 hrs at free cells and immobilized cells. In our experiments, the using soils contained 23.16 g-P/kg-soil total phosphate and 3.76 g-P/kg-soil soluble phosphate. The result of 1g immobilized bead seeding, soluble phosphate was produced maximum 6.14 g-P/kg-soil phosphate and this value was increased continuously.

• 한국염색가공학회지
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• 제23권4호
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• pp.257-262
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• 2011

The dish cloth requires antimicrobial activity for hygienic purpose. Until now, organic antimicrobial agents of low cost have been frequently used for the anitimicrobial finishing. However, a cleaner hving anitimicroial activity should be more durable considering consumers' ever-increasing expectation. In this study, the cleaner containing the bamboo fiber were finished with a formulation of a new zirconium-containing inorganic agent of zirconium phosphate and polyurethane binders. The optimum treatment condition was investigated. And its durability against repeated laundering cycles was assessed. The existence of Zr after the repeated laundering was verified and the antimicrobial effect did not decrease under 30 washing cycles.

• Applied Biological Chemistry
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• 제44권4호
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• pp.251-256
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• 2001

난용성 인산염을 가용화 시킬 수 있는 경제적이고 효율적인 생물비료를 개발하기 위하여 난용성 인산염 가용화능이 우수한 미생물을 염류 집적 및 인산과다처리 재배지로부터 분리하였다. 이 분리균주의 생리 생화학적인 특성을 조사한 결과 Aeromonas hydrophila DA57로 동정되었다. Aeromonas hydrophila DA57은 hydroxyapatite, tri-calcium phosphate 와 같은 난용성 인산염을 모두 가용화한다. 난용성 인산염의 분해능이 최대가 되는 배양 온도와 배양초기 pH는 각각 $30^{\circ}C$와 5.0이었으며, 탄소원으로 glucose를 3% 첨가시 가용화능이 높았다. 배양 pH가 감소함에 따라 가용화된 유리인산의 함량이 증가하였고 gluconic acid의 생성이 확인되었다. 따라서 난용성 인산염의 분해가 우수한 새로운 Aeromonas hydrophila DA57를 분리하였고, 이는 하나의 생물비료원으로 활용이 가능하리라고 사료된다.

• 펄프종이기술
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• 제44권1호
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• pp.52-57
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• 2012

In this study, we investigated the possible use of a new inorganic material as alternative filler in the paper industry. The inorganic material is a mineral composed of calcium sulfate, that is generated when manufacturing phosphate fertilizer. The inorganic material was dehydrated by the thermal treatment to $200^{\circ}C$, $500^{\circ}C$, $700^{\circ}C$, and $900^{\circ}C$ to prepare white filler powders. Their basic properties, including color, particle shape, elements, and average particle size were identified. To determine the effect of new inorganic filler on paper's physical properties and strength, handsheets were prepared from HwBKP, SwBKP, and thermal treated inorganic fillers. Handsheets' ISO brightness, opacity, bulk, breaking length, and stiffness were measured. Results confirmed that thermal treated inorganic filler could be beneficial to the bulk and opacity of paper while maintaining higher level of breaking length and stiffness that is achieved using talc.

• 한국연초학회지
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• 제1권1호
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• pp.1-8
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• 1979

담배 (Va 115) 細胞의 液 培養에서 담배細胞의 多量生産을 할 수 있는 Tank 培養에 關한 基礎調査로 培地造成의 果를 調査하였으며 細胞增殖率에 큰 影響을 미쳤던 2,4-D와 無機燐酸의 果에 關하여 調査하였다. 1. 培地造成에서 細胞增殖率에 많은 影響을 미쳤던 要素는 무糖, 無機燐酸의 濃度,窒素源의 形態 및 植物홀몬,特히 2, 4-D의 有無였다. 2. 무糖의 最適濃度는 3%였으며 3%以上의 濃度에서는 多少 細胞增殖率이 좋은 듯 하였으나 그 差異는 크지 않아 3% 程度로 足하였다. 無機燐酸濃度는 LS 培地內의 無機燐酸의 約 2.5 培인 0.30mg/ml 일 때 細胞增殖率이 가장 좋았다. 3. 液 培地의 窒素源은 암모니아態窒素와 硝酸態窒素가 1 : 2 일 때가 가장 좋았고 窒素源이 암모니아태 만으로 使用하였을 때 細胞增殖率이 가장 낮았으며 硝酸態만 使用되었을 때는 암모니아태만 쓰였을 때 보다는 좋았으나 암모니아태와 硝酸態의 比가 1 : 2 일 때 보다는 떨어졌다. 4. 液 培地에 2,4-D 添加와 無機燐酸濃度를 높이면 細胞增殖率이 增加되는 機作을 調査하기 爲하여 呼吸率과 14C - glucose 吸收利用을 調査하였다. 細胞의 吸收率은 2, 4-D를 添加하면 增加되며 14C-glucose의 吸收는 培地內에 2, 4-D가 包含되거나 (0.2 ppm) 燐酸濃度가 높아지면(對照의 2.5培) 더욱 많았고, 吸收된 14C-glucose는 糖 상태보다 다른 形態,特히 amino 酸이나 有機酸으로 많이 变하였다.

• 한국가축번식학회지
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• 제20권3호
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• pp.251-258
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• 1996

본 연구는 착성전 Rat 8-세포기 난자의 체외배양시 glucose95.56 mM와 0mM)와 phosphate(1.19mM와 0 mM)의 억제효과를 규명하기 위하여 실시하였다. 48시간 배양후 발육율은 glucose＋phosphate군에서는 37%(31/84), glucose군은 70%(64/91), phosphate군은 69% (59/85) 그리고 glucose와 phosphate가 없는 무처리군에서는 77%(67/85)를 나타냈다. Glucose＋phosphate군의 발육율은 다른 처리군에 비해 유의적으로 낮았고, 그 외 처리군 사이에는 유의성이 없었다. 발육난자의 핵염색에 의한 세포수의 측정에서는 glucose군에서 가장 많은 세포수를 나타냈고 (29.3$\pm$0.97, P<.001), 그외 처리에서는 유의성이 없었다 (glucose＋phosphate, 17.5$\pm$1.04; phosphate, 18.6$\pm$1.01; no glucosephosphate, 19.8$\pm$1.01). 본 실험의 결과로 glucose와 phosphate는 Rat 8-세포기 난자의 발육에 억제효과를 나타냈고, glucose는 난자의 세포분열을 증진시키는 효과가 있는 것으로 나타났다.

• 한국초지조사료학회지
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• 제16권2호
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• pp.133-138
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• 1996

To investigate the effects of inorganic phosphate ($P_2O_5$ 200kg/ha), fermented cow manures($P_2O_5$ 200kg/ha, 400kg/ha) and poultry manures($P_2O_5$ 200kg/ha, 400kg/na) levels mixed with sawdust on the soil changes and productivity of alfalfa monoculture, a field experiment arranged by randonmized complete block design was conducted at National Livestock Research Institute in Suwon, September, 1993 to December 1995. The results are as follows. S1. Soil pH was changed from 5.40(before experiment) to 6.17(fermented cow manure, $P_2O_5$ 400kg/ha), 6.76 (fermented poultry manure, $P_2O_5$400kg/ha) and 6.3l(inorganic phosphate, $P_2O_5$ 200kg/ha) at 0~5cm depth of subsoil, respectively. Organic contents after experiment were lower as a range of 2.41%~3.15% than those before experiment. 2. Total nitrogen contents of each treatment showed as 0.13%~0.16% at 0~5cm depth of subsoil comparing with before experiment(0.15%). Available phosphate content of inorganic phosphate($P_2O_5$200kgha) was highest as 405ppm. lowest as 125ppm(fermented poultry manure, $P_2O_5$ 200kg/ha) than before trial(205ppm). 3. K and Ca contents of after experiment were appeared to 0.22%~0.78% and 6.45%~9.45% comparing with before experiment(0.10% and 3.56%), respectively. 4. During two years, average DM yield of fermented cow rnanure($P_2O_5$ 400kg/ha) was highest as 11, 731kg/ha and lowest as 9, 017kg/ha at fermented poultry manure plot($P_2O_5$ 200kg/ha) with as sawdust, but no significant difference was found among treatment(P>0.05). 5. CP production, NEL and TDN yield of fermented cow manure($P_2O_5$ 400kg/ha) were highest as 2, 282kg/ha 69, 900kg/ha and 6, 392kg/ha, respectively, but no significant difference among treatment(P>0.05). 6. In inorganic contents of alfalfas, there was not show same tendency in accordance with treatments. As a results mentioned above, it was possible to use 200kg $P_2O_5/ha$ of fermented cow or poultry manure with sawdust instead of inorganic phosphae fertilizer.

• Journal of Microbiology and Biotechnology
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• 제7권2호
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• pp.127-131
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• 1997

Alkaline phosphatase (APase) was found to be inducible in Anabaena sp. strain CA Growth was less than control in presence of most amino acids except glycine and serine, but most amino acids enhanced APase activity. Highest APase activity was recorded in tyrosine supplemented culture followed by hydroxyproline, cystein, valine and glutamic acid. Threonine supplemented material showed lowest APase level (1.8 nmol/mg protein/min). Lactose, glucose, sodium pyruvate and succinate stimulated growth but not APase activity. APase activity was high in the presence of sucrose, mellibiose, mannitol, arabinose, maltose and sorbose, even though the growth in these supplements was less than in control. Organic phosphate sources supported good growth of the organism. Best growth occurred in presence of inorganic phosphate, adenosine diphosphate, fructose 1,6-diphosphate or ribulose 1,5-diphosphate, followed by other phosphorus sources tested. APase activity in presence of any of the organic phosphate sources was 3 to 5 fold low as compared to phosphate limited culture. Also, there was no APase activity in cultures grown on inorganic phosphate. These data indicate that most amino acids and a few carbohydrates (sucrose, mellibiose, arabinose and sorbose) are suitable for APase production. Lactose, glucose, pyruvate or succinate may be used as a carbon source during photoheterotrophic growth of the cyanobacterium. Glycine and serine are preferred nitrogen sources for its growth. Phosphate repressible APase activity has been found in Anabaena sp. strain CA.