• 대한정형외과스포츠의학회지
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• 제1권1호
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• pp.55-60
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• 2002

목적 : 토끼후방십자인대의절단뒤후방십자인대의치유와기계적수용기의수적변화에대해관찰하고자하였다. 대상및방법 : 골성숙이완료된열마리의뉴질랜드산흰토끼를 이용하여각각에서양측슬관절중한쪽슬관절에서는후방십자인대를절단하고, 다른한쪽에는샴술기를시행한후20주후에생검하여후방십자인대의치유가이루어진9례에서조직학적검사를시행하여후방십자인대를절단한슬관절과샴술기를시행한슬관절을비교하였다. 결과 : 후방십자인대절단후치유가이루어진조직들에서샴술기를시행하였던조직보다기계적수용기가약간감소한것으로관찰되었으나통계적유의성은없었다. 결론 : 후방십자인대가손상된슬관절에서비록기계적수용기가감소한다할지라도고유감각이유지될수있을것으로사료되나기계수용기의손상된후방십자인대에대한작용과생역학적기능에대해서는좀더연구가이루어져야될것으로사료된다.

• Restorative Dentistry and Endodontics
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• 제24권2호
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• pp.372-380
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• 1999

The purpose of this study was to investigate the distribution of calcitonin gene-related peptide(CGRP) containing nerve fivers after pulp exposure in rats. The Spague-Dawley rats weighing about 250 - 300g were used. The animals were devided into normal control group and experimental groups. Experimental animals were sacrified on 2, 4, 7, 10 days after pulp exposure. The maxillary teeth and alveolar bone were removed and immersed in the 4% paraformaldehyde plus 0.1M phosphate buffer (pH 7.4). Serial frozen $50{\mu}m$ thick sections were cut with a cryostat. In the immunohistochemical staining procedure, the rabbit CGRP antibody was used as a primary antibody. The sections were incubated for 48 hours at $4^{\circ}C$, and placed into biotinylated anti-rabbit IgG as a secondary antibody and incubated in ABC (avidin-biotin complex), The sections were visualized by 0.05% 3.3 diaminobenzidine tetrahydrochloride. The results of this study were as follows: 1. In control group, CGRP containing nerve fibers ran parallel to the long axis of root and reached the coronal pulp. They were distributed on Raschkow plexus under the odontoblastic layer. 2. In 2 day group after pulp exposure, tissue necrosis and acute inflammation occurred and CGRP containing nerve fibers increased. In 4 day group, the necrotic tissue extended to the pulp and CGRP containing nerve fibers were distributed around the inflammation zone. 3. In 7 day group after pulp exposure, pulp necrosis occurred, and in 10 day group, the abscess under the necrotic pulp extended to the root apex area and CGRP containing nerve fibers were not observed in root canals. 4.The sprouting of CGRP nerve fibers was most remarkable at the pulp chamber under injury in 4 day group, and it was found at inflammation zone under the necrotic tissue in 7 day group and the remaining root pulp tissue in 10 day group. As mentioned above, CGRP nerve fibers had a tendency to increase around the inflammatory zone, especially around the acute inflammation tissue, when compared with control group. It is suggested that CGRP nerve fibers maybe related to the control of inflammatory response of pulp tissue.

• Journal of Chest Surgery
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• 제44권1호
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• pp.44-50
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• 2011

Background: Cardiovascular complications are major causes of morbidity and mortality following non-cardiac thoracic operations. Recent studies have demonstrated that elevation of N-Terminal Pro-B-type natriuretic peptide (NT-proBNP) levels can predict cardiac complications following non-cardiac major surgery as well as cardiac surgery. However, there is little information on the correlation between lung resection surgery and NT-proBNP levels. We evaluated the role of NT-proBNP as a potential marker for the risk stratification of cardiac complications following lung resection surgery. Material and Methods: Prospectively collected data of 98 patients, who underwent elective lung resection from August 2007 to February 2008, were analyzed. Postoperative adverse cardiac events were categorized as myocardial injury, ECG evidence of ischemia or arrhythmia, heart failure, or cardiac death. Results: Postoperative cardiac complications were documented in 9 patients (9/98, 9.2%): Atrial fibrillation in 3, ECG-evidenced ischemia in 2 and heart failure in 4. Preoperative median NT-proBNP levels was significantly higher in patients who developed postoperative cardiac complications than in the rest (200.2 ng/L versus 45.0 ng/L, p=0.009). NT-proBNP levels predicted adverse cardiac events with an area under the receiver operating characteristic curve of 0.76 [95% confidence interval (CI) 0.545~0.988, p=0.01]. A preoperative NT-proBNP value of 160 ng/L was found to be the best cut-off value for detecting postoperative cardiac complication with a positive predictive value of 0.857 and a negative predictive value of 0.978. Other factors related to cardiac complications by univariate analysis were a higher American Society of Anesthesiologists grade, a higher NYHA functional class and a history of hypertension. In multivariate analysis, however, high preoperative NT-proBNP level (>160 ng/L) only remained significant. Conclusion: An elevated preoperative NT-proBNP level is identified as an independent predictor of cardiac complications following lung resection surgery.

• Journal of Trauma and Injury
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• 제20권1호
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• pp.52-56
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• 2007

Marjolin's ulcer is a rare and often-aggressive cutaneous malignancy that arises in previously traumatized or chronically inflamed skin, particularly after burns. We experienced two cases after burns. Case I involved a forty eight year-old man who had suffered from a flame burn at the parietal scalp area, where had been initially described three years earlier as a full-thickness wound including the pericranium. The man consulted us for a persistent ulcerative and infected wound on the burned lesion during the last 24 months, which turned out on the contrast-enhanced computed tomography (CT) and magnetic resonance imaging (MRI) to be the squamous cell carcinoma with involving the skull and the dura mater. Although the posterior auricular lymph node was enlarged on the ipsilateral side, recent positron emission tomography (PET) CT did not show any metastatic lesion. It was impossible for us to resect the intracranial involvement of the tumor radically, and the postoperative PET CT still showed a focal fluorodeoxyglucose (FDG) uptake around the wall of the superior sagittal sinus. We think that an aggressive combined approach is essential for treatment in early stages for a high success rate, before the intracranial structures are involved because there is no consensus on the treatment for advanced disease, and the results are generally poor. Case 1 also did not involve a radical resection because of the intracranial invasion to the wall of superior sagittal sinus and the possibility of damage to the major cortical veins. He received adjuvant radiotherapy and must be followed periodically. Case 2 involved an eighty six year-old women who suffered from a painful scalp ulcer lesion after flame burns three years earlier. Unlike case 1, neither tumor infiltration into the dura nor lymph node enlargement was observed on the contrast-enhanced computed tomography (CT), magnetic resonance imaging (MRI), or positron emission tomography (PET) CT. We did a radical resection of the tumor, including the involved bone, and a cranioplasty with bone cement.

• 한국유기농업학회지
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• 제7권1호
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• pp.35-48
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• 1998

고추 연작지에서의 녹비작물의 효과를 구명하기 위하여 자운영을 포함한 5개 녹비작물을 동계간에 재배한 결과 자운영은 월동한 개체가 정연없이 동계간에 모두 동사하였으며, 나머지 4종류 녹비작물의 건물중은 호밀> 밀> 보리> 이탈리안 라이그라스의 순이었는데, 호밀의 경우 ha당 건물중이 4.1통이었다. 녹비작물 재배는 고추의 연작장해, 특히 고추 연작지에서 가장 문제가 되는 고추 역병(Phytophthora capsis)의 발생을 경감시키는 효과가 있는 것으로 나타났으며, 果長과 果直徑이 증가하여 고품질의 고추생산이 가능하였다. 1년차로 퇴비만을 사용한 경우 고추의 생육과 수량은 화학비료를 사용한 구에 비하여 떨어지는 결과를 보였으나 퇴비 사용량이 증가할수록 그 차이가 줄어들었으며, 토양중의 유기물 함량이 높아져 퇴비의 지속적 肥效가 인정되었다. 고추영농의 경우 저투입지속농업(LISA)과 일반관행농업을 비교해 보면, 수익-비용 분석결과를 중심으로 한 주요 경제적요인과 기술적요인 양측 모두 뚜렷한 차이가 있었다. 따라서 LISA에 의한 고추영농을 발전시키려면 단순히 기술진보 뿐만 아니라 다양한 현실적 목표를 조화시킬 수 있는 LISA 고추영농에 필요한 다목표 의사결정모형을 개발할 필요가 있는 것으로 나타났다.

• Archives of Reconstructive Microsurgery
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• 제19권2호
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• pp.97-100
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• 2010

Purpose: Epidermolysis bullosa is a rare genetic disease, characterized by the presence of extremely fragile skin and formation of recurrent blister resulting from even a minor mechanical injury. Squamous cell carcinoma (SCC) is recognized as a complication of the chronic scarring associated with dystrophic epidermolysis bullosa (DEB). When a soft tissue defect happens in a patient with epidermolysis bullosa, it is difficult to cover it with a skin graft or a flap. We describe the successful use of a pedicled deep inferior epigastric perforator flap for the reconstruction of SCC associated with DEB in the groin. Methods: A 29-year-old man diagnosed with DEB at birth sustained an ulcer increasing in the right groin for the last 7 months. Under general anesthesia, the mass lesion and lymph nodes were removed and the resulting defect was covered with a pedicled deep inferior epigastric perforator flap. Results: The flap survived completely and his postoperative course was uneventful. Histopathological examination revealed a SCC in the right groin and malignant tumor cells in the removed lymph nodes as well. Additional positron emission tomogram showed a malignant lesion in the ileocecal area with regional lymph node metastasis. The patient was referred to an oncologist for chemotheraphy, but the patient refused to take it. During a 4-month follow-up period, there was no recurrence in the right groin. Conclusion: We suggest that perforator flaps can be considered as a reliable alternative for the reconstruction of soft tissue defects in a patient with DEB.

• 원예과학기술지
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• 제33권1호
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• pp.55-60
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• 2015

과피흑변은 '부유' 단감(Diospyros kaki, 'Fuyu') 과실의 저장 중에 발생하는 주요 생리적 장해의 하나이다. 부유 과실의 과피에서 페놀화합물의 함량, PPO와 POD의 활성, 지질 포화도를 분석하였다. 페놀화합물은 일광의 방향에 따라 과피에 불균등하게 분포하였는데, 일광 노출면에 축적량이 가장 많았고 그 반대면에서는 가장 적었다. 그러나 수관 내부의 음지 가지에서 수확한 과실의 경우 페놀화합물은 과피에 비교적 고르게 분포되어 있었다. PPO와 POD의 활성 또한 과피에서 고르지 않았으나, 이는 과피흑변이 발생한 과실에서만 그러하였으며, 흑변 부위는 PPO 활성은 가장 높고 POD 활성은 가장 낮았다. 수확 시에 일광면에 표식을 한 과실을 저온에 저장하였을 때, 저장 중 과피흑변의 증상인 운형 흑반은 정확히 표식 부위에 발생하였다. 게다가, 일광면은 높은 지질포화도를 보였는데, 이는 일광에 의해 야기된 고온에 대한 적응을 의미한다. 따라서 이러한 결과로 미루어 볼 때, 부유 과실의 과피흑변 장해는 일광에 노출된 과실의 고온 순화 과피 조직에서 나타나는 저온장해 증상의 일종인 것으로 간주할 수 있을 것이다.

• The Korean Journal of Physiology and Pharmacology
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• 제23권2호
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• pp.151-159
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• 2019

Pruritus (itching) is classically defined as an unpleasant cutaneous sensation that leads to scratching behavior. Although the scientific criteria of classification for pruritic diseases are not clear, it can be divided as acute or chronic by duration of symptoms. In this study, we investigated whether skin injury caused by chemical (contact hypersensitivity, CHS) or physical (skin-scratching stimulation, SSS) stimuli causes initial pruritus and analyzed gene expression profiles systemically to determine how changes in skin gene expression in the affected area are related to itching. In both CHS and SSS, we ranked the Gene Ontology Biological Process terms that are generally associated with changes. The factors associated with upregulation were keratinization, inflammatory response and neutrophil chemotaxis. The Kyoto Encyclopedia of Genes and Genomes pathway shows the difference of immune system, cell growth and death, signaling molecules and interactions, and signal transduction pathways. Il1a, Il1b and Il22 were upregulated in the CHS, and Tnf, Tnfrsf1b, Il1b, Il1r1 and Il6 were upregulated in the SSS. Trpc1 channel genes were observed in representative itching-related candidate genes. By comparing and analyzing RNA-sequencing data obtained from the skin tissue of each animal model in these characteristic stages, it is possible to find useful diagnostic markers for the treatment of itching, to diagnose itching causes and to apply customized treatment.

• 디지털융복합연구
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• 제17권2호
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• pp.217-230
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• 2019

본 연구는 기존 동반질환을 이용한 중증도 보정 방법의 제한점을 보완하기 위해 급성심근경색증 환자의 맞춤형 중증도 보정방법을 개발하고, 이의 타당성을 평가하기 위해 수행되었다. 이를 위하여 질병관리본부에서 2006년부터 2015년까지 10년간 수집한 퇴원손상심층조사 자료 중 주진단이 급성심근경색증인 한국표준질병사인분류(KCD-7) 코드 I20.0~I20.9의 대상자를 추출하였고, 동반질환 중증도 보정 도구로는 기존 활용되고 있는 CCI(Charlson comorbidity index), ECI(Elixhauser comorbidity index)와 새로이 제안하는 CCS(Clinical Classification Software)를 사용하였다. 이에 대한 중증도 보정 사망예측모형 개발을 위하여 머신러닝 기법인 로지스틱 회귀분석, 의사결정나무, 신경망, 서포트 벡터 머신기법을 활용하여 비교하였고 각각의 AUC(Area Under Curve)를 이용하여 개발된 모형을 평가하였다. 이를 평가한 결과 중증도 보정도구로는 CCS 가 가장 우수한 것으로 나타났으며, 머신러닝 기법 중에서는 서포트 벡터 머신을 이용한 모형의 예측력이 가장 우수한 것으로 확인되었다. 이에 향후 의료서비스 결과평가 등 중증도 보정을 위한 연구에서는 본 연구에서 제시한 맞춤형 중증도 보정방법과 머신러닝 기법을 활용하도록 하는 것을 제안한다.

• 한국초지조사료학회지
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• 제41권2호
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• pp.77-83
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• 2021

'중모2509'는 국내의 재배환경에 어울리고, 조사료용이지만 종자가 균일하여 식용으로의 활용이 가능한 국내 최초의 자식계 호밀 품종이다. 품종은 1999년도에 자가불화합성으로 타식성 품종인 '올호밀'을 모본으로 하고, 자가화합성으로 자식계인 'Synthetic II'을 부본으로 인공교배하여 만들었다. '중모2509'는 중간 크기의 녹색이고 다소 긴 잎, 황백색 줄기와 작고 황갈색인 종실을 가졌다. '중모2509'는 출수기가 4월 23일로 기존의 타가 수정 품종인 '곡우'의 4월 18일보다 5일 늦지만, 내한성·습해·병해는 대등하였으며, 도복은 강하였다. 건물수량은 ha당 7.60톤(생초수량 35.2톤)이고, 종실수량이 2.08톤으로 '곡우'보다 적었다. 그러나 자식에 의해 종자가 생산된 자식률은 56 %로, 자식에 의해 임실이 되지 않은 '곡우'와 대조되었다. '중모2509'는 조단백질 함량(9.4 %), 가소화영양소총량(55.7 %)이 표준품종인 '곡우'(8.0 %, 55.0 %) 보다 높았다. '중모2509'는 초형이 직립이고 내도복성이기 때문에 밀식 기계화 재배에 유리한 장점이 있다. 향후 이 품종은 순도 높고 종실수량이 많은 식용 및 조사료용 호밀 품종육성을 위한 중간모본으로 활용과 더불어 고품질 원료의 요구도가 높은 식가공 분야에 균일·안정성이 확보된 국내산 호밀 종자로 제공이 기대된다.