• Parasites, Hosts and Diseases
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• v.37 no.4
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• pp.265-270
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• 1999

The gene encoding Plasmodium vivax circumsporozoite protein (PvCSP) exhibits polymorphism in many geographical isolates. The present study was designed to investigate polymorphism in PvCSP gene of P. vivax isolates in Korea. Thirty isolates, obtained from indigenous cases in Yonchon-gun, Kyonggi-do in 1997, were subjected for sequencing and RFLP analysis of the repeat and post-repeat regions of PvCSP gene and two genotypes (SK-A and SK-B) were identified. The genotype of 19 isolates was SK-A and that of 11 isolates was SK-B. Although the number of 12-base repeats present in SK-A was three while two were found in a Chinese strain CH-5, the repeat sequence of SK-A was identical to that of CH-5 except for one base substitution. Compared with known data there was no identical isolates with SK-B, but the sequence of SK-B was similar to that of a North Korean (NK) isolate. These results indicate that two genotypes of PvCSP coexist in the present epidemic area of Korea and the present parasite may originate from East Asia. RFLP would be useful to classify genotypes of P. vivax population instead of gene sequencing.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1670-1674
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• 2007

Duffy binding protein (DBP) plays a critical role in Plasmodium vivax invasion of human red blood cells. We previously reported a single-chain antibody fragment (scFv) that was specific to P. vivax DBP (PvDBP). However, the stabilization and the half-life of scFvs have not been studied. Here, we investigated the effect of PEGylated scFvs on their biological activity and stability in vitro. SDS-PAGE analysis showed that three clones (SFDBII-12, -58, and -92) were formed as monomers (about 70 kDa) with PEGylation. Clone SFDBII-58 gave the highest yield of PEGylated scFv. Binding analysis using BIAcore between DBP and scFv showed that both SFDBII-12 and -58 were decreased approximately by two folds at the level of binding affinity to DBP after PEGylation. However, the SFDBII-92 clone still showed a relatively high level of binding affinity ($K_D=1.02{\times}10^{-7}\;M$). Binding inhibition assay showed that PEGylated scFv was still able to competitively bind the PvDBP and playa critical role in inhibiting the interactions between PvDBP protein expressed on the surface of Cos-7 cells and Duffy receptor on the surface of erythrocytes. When both scFvs and their PEGylated counterparts were exposed to trypsin, scFv was completely degraded only after 24 h, whereas 35% of PEGylated scFvs remained intact, maintaining their stability against the proteolytic attack of trypsin until 72 h. Taken together, these results suggest that the PEGylated scFvs retain their stability against proteolytic enzymes in vivo, with no significant loss in their binding affinity to target antigen, DBP.

• Korean Journal of Environment and Ecology
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• v.7 no.1
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• pp.29-34
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• 1993

This study was conducted to investigate to compare avifauna at Taegosa area in Pukhansan with Kamapong area in Inje-gun, Korea. The observed birds at Taegosa in Pukhansan and Kamapong area were 35 individuals on species and 113 individuals on 29 species respectively. In Pukhansan area, there was severely avifauna diminishing in July in which users were concentrated. Pica pica sericea and Streptopelia orientalis orientalis were observed the dominant species in Pukhansan area and Kamapong area respectively. There were two species of national monument in these surveyed area, Otus scops stictonotus in Pukhansan area and Falco tinnunculus interstinctus in Kamapong area. Cuculus saturatus horsfields, Cuculus micropterus micropterus and Eurystomus orientalis calonyx were found out in Kamapong area to the rare bird. Species diversity and avi-ecosystems in Kamapong area were seemed to good, whereas in Pukhansan area, there was relatively poor avifauna caused by isolating with surrounding ecosystem, overusing by visitors beyond capacity and heavy acid rain. Therefore avifauna protection plans sould be made and carried out for Pukhansan National Park by thorought study with experts.

• Journal of Korean Society of Rural Planning
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• v.23 no.1
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• pp.147-156
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• 2017

This study suggests a plan to expand the rural experience program linked to natural disaster through the case analysis of the village of Garisan-ri, Inje-gun, Gangwon-do, Republic of Korea. This will help to establish the necessary improving and policies for Re-leap of rural villages in disaster areas and activation of rural experience villages. The local residents have created flood recovery stories, disaster response content, and disaster prevention camp sites, all of which have been hit by serious disaster damage. It has also contributed to the revitalization of the village by providing various experience programs such as disaster response training, crossing rapids, and evacuation experience. This is a way to get rid of the negative perception of disaster and utilize it. The conversion of idea through disaster experience content contributed to the activation of the area. By linking the disaster experience contents to the rural experience program in the future, it can be helpful to avoid the duplication of contents and limit the similar operation method, which is an existing problem of rural experience villages.

• Journal of The Geomorphological Association of Korea
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• v.19 no.2
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• pp.113-122
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• 2012

The geomorphic properties of potholes at Naerin River, Inje-gun are analyzed to discover the geosites of DMZ geopark in Gangwon-do. The furrow-type potholes along the river flow are mainly found in the P1 and P2 sites. The P3 site can be divided in to the zones where the furrow-type potholes densely distribute and various types of potholes are scattered, while the various types of small potholes are scattered in the P4 site. After measuring the 44 potholes, the potholes with a U-shaped cross-section, similar directivity with river flow and deposited gravels with in them show large values in length, width, depth and slope. Because the P1 and P2 sites are characteristic in the scales and forms of potholes and show beautiful scapes, it can be suggested that they are suitable for the geosite developments.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.35 no.4
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• pp.236-242
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• 2013

Purpose: The objective of this retrospective study is to evaluate the factors affecting the spread of odontogenic infection. Furthermore, this study was performed to apply to future treatments. Methods: A total of 65 patients, who had received treatment for odontogenic infections from 2010 to 2012 for 3 years, were enrolled in this study. The causes of infection, presence of systemic disease, and complications, durations of treatment, treatment methods, and inflammation levels were compared with the data. Results: Patients over 70 years with systemic disease required immediate drainage, systemic antibiotic therapy and hospitalization. We can determine the direction of the early diagnosis and treatment through blood tests (white blood cells, neutrophil, C-reactive protein [CRP]) and computed tomography. Patients over 70 years with systemic disease had the highest percentage. In addition, these patients showed high levels of inflammation index, such as CRP average of 24.8 and needed for a long-term treatment period and a wide range of surgical incision & drainage several times. Systemic diseases, particularly diabetes mellitus and hypertension, accelerate the spread of infection and had a negative effect that delays healing. Eventually, five of the 65 patients showed serious systemic complications. Conclusion: When evaluating cervico-facial infected patients due to odontogenic infection, the most important thing is deciding the appropriate diagnosis and degree of disease. Considering the patient's systemic status and age, we need to decide the treatment plan. Especially, those patients over 70 years with systemic disease should be treated with rapid surgical approach, and the use of a wide range of antibiotics and intensive care. If proper treatment principle does not apply, severe life-threatening complications will result, such as necrotizing fascitis, acute airway obstruction, mediastinitis, and others.

• Journal of Korean Orthopaedic Sports Medicine
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• v.4 no.2
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• pp.109-115
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• 2005

Purpose: The purpose of this study was to evaluate the effectiveness of three functional performance tests and one-legged hop test and to establish the reference data of the three functional performance tests of the active young Korean men. Materials and methods: We performed five tests on 40 normal subjects such as one-legged hop test (single hop, triple hop) and the three functional performance tests (carioca test, co-contraction test, shuttle run test). The mean age was 28.4 years. The mean score of Tegner activity scale was 6.2 and Lysholm score 98.2 Mean value and standard deviation were obtained. The correlation of age, height and weight with the results, the relationship between FPT and one-legged hop test and the mean difference between dominant and non-dominant limbs were statistically calculated. Results: All data obtained were normally distributed and there were no significant relationship between age, height and weight. The paired t-test of one legged hop test showed no statistical significance between dominant and non-dominant limbs. The results of one legged hop test had strong relationship with functional performance test. Conclusion; The three functional performance tests can be a useful functional test in ACL-insufficient athletes. Our results can be used as normal reference data of the normal active Korean men.

• Journal of Environmental Science International
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• v.16 no.8
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• pp.995-999
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• 2007

This study assessed the characteristics of emission and cell toxicology of Methylethylketone(MEK) in ambient air of industrial area. MEK is produced by the oxidation of sec-butyl alcohol and used as the solvent for making ink, printing, coating of film, bonding material and drug extraction. The MEK concentrations in the ambient-air of industrial area in Gimhae City was detected in the range of $25.4{\sim}1,580{\mu}g/m^3$ with an average $297.4{\mu}g/m^3$. The concentration of MEK showed a descending tendency from April to August followed by its increased tendency since then. The effects of MEK on the human lung cancer A549 cells was examined by the generation of Reactive Oxygen Species(ROS) and cytotoxicity. The range of MEK concentration detected in the area induced ROS generation affecting the oxidation state with a little effects on the viability of the cells.

• Journal of agricultural medicine and community health
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• v.20 no.1
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• pp.15-23
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• 1995

As compared before, agricultural machines are used more commonly instead of animal or manpower in rural areas and the injuries due to those are common. This study was conducted by questionnaire method in order to find out the current status of injuries due to agricultural machines for farmers who was selected from three Gun's in Kyeongsangnam Province. The study subjects were 385 persons in all(210 male persons, 175 female reasons) and the study period was from July through September 1993. The results were as follows; 1. The injury rate due to agricultural machines was high in male(p<0.05), and it was higher in younger age group and higher educated group. 2. The injury occurred high in summer and autumn seasons(77.6%), in the afternoon(60.6%), and during harvest(35.2%). 3. The major injuries were contusion, fracture, amputation in order and the injured sites were arms, legs, and chest in order. 31.7% of the injured farmers had been admitted, and they were treated at hospitals, home, drugstores and health centers in order. 4. The casualty damage was highest by cultivators, and agricultural instruments, threshing machine were followed. Among traumatic injuries concerned with cultivators contusions were most common, and fractures, amputations were followed. In case of agricultural instruments bruises were most common, and incisions, contusion were followed. In case of threshing machines fractures were most common and contusion, bruise were followed.

• Journal of the Korean Society of Safety
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• v.31 no.1
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• pp.105-110
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• 2016

Mountainous disasters such as landslides and debris flow are difficult to forecast. Debris flow in particular often flows along the valley until it reaches the road or residential area, causing casualties and huge damages. In this study, the researchers selected Seoraksan National Park area located at Inje County (Inje-gun), Gangwon Province-where many mountainous disasters occur due to localized torrential downpours-for the damage reduction and cause analysis of the area experiencing frequent mountainous disasters every year. Then, the researchers conducted the field study and constructed geospatial information data by GIS method to analyze the characteristics of the disaster-occurring area. Also, to extract more precise geographic parameters, the researchers scanned debris flow triggering area through terrestrial LiDAR and constructed 3D geographical data. LiDAR geographical data was then compared with the existing numerical map to evaluate its precision and made the comparative analysis with the geographic data before and after the disaster occurrence. In the future, it will be utilized as basic data for risk analysis of mountainous disaster or disaster reduction measures through a fine-grid topographical map.