• Parasites, Hosts and Diseases
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• 제37권4호
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• pp.265-270
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• 1999

The gene encoding Plasmodium vivax circumsporozoite protein (PvCSP) exhibits polymorphism in many geographical isolates. The present study was designed to investigate polymorphism in PvCSP gene of P. vivax isolates in Korea. Thirty isolates, obtained from indigenous cases in Yonchon-gun, Kyonggi-do in 1997, were subjected for sequencing and RFLP analysis of the repeat and post-repeat regions of PvCSP gene and two genotypes (SK-A and SK-B) were identified. The genotype of 19 isolates was SK-A and that of 11 isolates was SK-B. Although the number of 12-base repeats present in SK-A was three while two were found in a Chinese strain CH-5, the repeat sequence of SK-A was identical to that of CH-5 except for one base substitution. Compared with known data there was no identical isolates with SK-B, but the sequence of SK-B was similar to that of a North Korean (NK) isolate. These results indicate that two genotypes of PvCSP coexist in the present epidemic area of Korea and the present parasite may originate from East Asia. RFLP would be useful to classify genotypes of P. vivax population instead of gene sequencing.

• Journal of Microbiology and Biotechnology
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• 제17권10호
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• pp.1670-1674
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• 2007

Duffy binding protein (DBP) plays a critical role in Plasmodium vivax invasion of human red blood cells. We previously reported a single-chain antibody fragment (scFv) that was specific to P. vivax DBP (PvDBP). However, the stabilization and the half-life of scFvs have not been studied. Here, we investigated the effect of PEGylated scFvs on their biological activity and stability in vitro. SDS-PAGE analysis showed that three clones (SFDBII-12, -58, and -92) were formed as monomers (about 70 kDa) with PEGylation. Clone SFDBII-58 gave the highest yield of PEGylated scFv. Binding analysis using BIAcore between DBP and scFv showed that both SFDBII-12 and -58 were decreased approximately by two folds at the level of binding affinity to DBP after PEGylation. However, the SFDBII-92 clone still showed a relatively high level of binding affinity ($K_D=1.02{\times}10^{-7}\;M$). Binding inhibition assay showed that PEGylated scFv was still able to competitively bind the PvDBP and playa critical role in inhibiting the interactions between PvDBP protein expressed on the surface of Cos-7 cells and Duffy receptor on the surface of erythrocytes. When both scFvs and their PEGylated counterparts were exposed to trypsin, scFv was completely degraded only after 24 h, whereas 35% of PEGylated scFvs remained intact, maintaining their stability against the proteolytic attack of trypsin until 72 h. Taken together, these results suggest that the PEGylated scFvs retain their stability against proteolytic enzymes in vivo, with no significant loss in their binding affinity to target antigen, DBP.

• 한국환경생태학회지
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• 제7권1호
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• pp.29-34
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• 1993

국립공원 북한산 중 태고사 지역과 강원도 인제군 가마봉 일대의 야생조류상을 비교하였다. 북한산 태고사 일대에는 12종 35개체, 인제군 가마봉 일대에서는 29종 113개체가 관찰되어 가마봉이 북한산보다 야생조류 분포가 다양하였다. 북한산에서는 이용객에 의한 서식간섭이 나타났으며 사람의 간섭에 비교적 강한 까치가 우점종으로 관찰되었다. 가마봉에서는 멧비둘기가 우점종이었으며 천연기념물 황조롱이를 비롯한 희귀종 벙어리뻐꾸기, 검은등뻐꾸기, 파랑새 등이 관찰되었다. 가마봉 일대의 야생조류는 종이 다양하고 서식환경이 안정된 것으로 보이나 북한산은 비교적 야생조류의 종이 단순할 뿐만 아니라 서식 환경도 매우 불안정하다고 사료된다. 이는 북한산이 생태적으로 고립되어 있고 이용객이 허용능력 이상 많으며 대기오염에 의한 산성비로 조류의 먹이가 현저히 줄어든데 그 원인이 있다고 판단된다. 따라서 국립공원 북한산에서 조류가 더 이상 감소되는 것을 막고 북한산의 자연생태계를 회복시킬 수 있는 보다 적극적이고 철저한 정책을 조속히 시행해야 할 것이다.

• 농촌계획
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• 제23권1호
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• pp.147-156
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• 2017

This study suggests a plan to expand the rural experience program linked to natural disaster through the case analysis of the village of Garisan-ri, Inje-gun, Gangwon-do, Republic of Korea. This will help to establish the necessary improving and policies for Re-leap of rural villages in disaster areas and activation of rural experience villages. The local residents have created flood recovery stories, disaster response content, and disaster prevention camp sites, all of which have been hit by serious disaster damage. It has also contributed to the revitalization of the village by providing various experience programs such as disaster response training, crossing rapids, and evacuation experience. This is a way to get rid of the negative perception of disaster and utilize it. The conversion of idea through disaster experience content contributed to the activation of the area. By linking the disaster experience contents to the rural experience program in the future, it can be helpful to avoid the duplication of contents and limit the similar operation method, which is an existing problem of rural experience villages.

• 한국지형학회지
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• 제19권2호
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• pp.113-122
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• 2012

본 연구는 강원도 DMZ 지오파크의 지오사이트를 발굴하기 위하여, 인제군 내린천의 포트홀을 대상으로 지형 특성을 분석하였다. 내린천의 P1, P2 지역은 유수의 방향을 따라 고랑형 와지인 퍼로우형 포트홀이 주로 나타나고, P3 지역은 퍼로우형 포트홀이 밀집된 구역과 여러 유형의 포트홀이 산재된 구역이 구분되며, P4 지역은 여러 유형의 소규모 포트홀이 산재되어 있다. 총 44개 포트홀을 계측한 결과, 단면이 U자형인 포트홀, 유향과 일치하는 방향성을 가진 포트홀, 내부에 자갈이 퇴적되어 있는 포트홀 등이 길이, 폭, 깊이, 경사도에서 모두 큰 값을 보이는 것으로 나타났다. 내린천의 P1, P2 지역은 포트홀의 규모와 형태가 매우 특징적이며 경관이 아름답기 때문에 지오사이트로 개발하기 양호한 곳으로 판단된다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제35권4호
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• pp.236-242
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• 2013

Purpose: The objective of this retrospective study is to evaluate the factors affecting the spread of odontogenic infection. Furthermore, this study was performed to apply to future treatments. Methods: A total of 65 patients, who had received treatment for odontogenic infections from 2010 to 2012 for 3 years, were enrolled in this study. The causes of infection, presence of systemic disease, and complications, durations of treatment, treatment methods, and inflammation levels were compared with the data. Results: Patients over 70 years with systemic disease required immediate drainage, systemic antibiotic therapy and hospitalization. We can determine the direction of the early diagnosis and treatment through blood tests (white blood cells, neutrophil, C-reactive protein [CRP]) and computed tomography. Patients over 70 years with systemic disease had the highest percentage. In addition, these patients showed high levels of inflammation index, such as CRP average of 24.8 and needed for a long-term treatment period and a wide range of surgical incision & drainage several times. Systemic diseases, particularly diabetes mellitus and hypertension, accelerate the spread of infection and had a negative effect that delays healing. Eventually, five of the 65 patients showed serious systemic complications. Conclusion: When evaluating cervico-facial infected patients due to odontogenic infection, the most important thing is deciding the appropriate diagnosis and degree of disease. Considering the patient's systemic status and age, we need to decide the treatment plan. Especially, those patients over 70 years with systemic disease should be treated with rapid surgical approach, and the use of a wide range of antibiotics and intensive care. If proper treatment principle does not apply, severe life-threatening complications will result, such as necrotizing fascitis, acute airway obstruction, mediastinitis, and others.

• 대한정형외과스포츠의학회지
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• 제4권2호
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• pp.109-115
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• 2005

목적: 본 연구의 목적은 슬관절의 외발 뛰기, 기능성 수행 평가의 유용성을 살펴보고 향후 기능 평가의 기준이 될 수 있는 한국 성인 남성의 평균값을 얻는데 있다. 연구대상 및 방법: 정상 성인 남성 40명을 대상으로 외발 뛰기(한번 뛰기, 세번 뛰기)와 3종 기능성 수행 평가(shuttle run, Carioca, Co-contraction test)등 총 5개 항목의 검사를 시행하였다. 피검자의 연령은 평균 28.4세었으며 Tegner activity score는 평균 6.2점, Lysholm score는 평 균 98.2점이었다. 각 검사 결과의 평균과 표준 편차를 구하였으며, 키, 몸무게, 연령과의 상관 관계, 우세 다리 (dominant limb)와 비 우세 다리(non-dominant limb)에서의 결과 차이, 뛰기 검사(hop test)와 기능성 수행 평가와의 상관 관계 등에 대하여 분석하였다. 결과: 검사한 5개 항목 모두 통계적으로 의미 있는 정규 분포를 하였으며, 키, 몸무게, 연령과는 상관 관계를 보이지 않았다. 외발 뛰기 검사는 우세 다리와 비 우세 다리에서 유의한 차이를 보이지 않았으며, 기능성 수행 평가와 유의한 상관 관계를 보였다(P<0.05). 결론: 3종 기능성 수행 평가는 전방 십자 인대 손상 환자의 기능 평가로 유용하며 본 연구의 결과는 정상 한국 성인 남성의 정상 기준 값으로 이용될 수 있을 것으로 판단된다.

• 한국환경과학회지
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• 제16권8호
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• pp.995-999
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• 2007

This study assessed the characteristics of emission and cell toxicology of Methylethylketone(MEK) in ambient air of industrial area. MEK is produced by the oxidation of sec-butyl alcohol and used as the solvent for making ink, printing, coating of film, bonding material and drug extraction. The MEK concentrations in the ambient-air of industrial area in Gimhae City was detected in the range of $25.4{\sim}1,580{\mu}g/m^3$ with an average $297.4{\mu}g/m^3$. The concentration of MEK showed a descending tendency from April to August followed by its increased tendency since then. The effects of MEK on the human lung cancer A549 cells was examined by the generation of Reactive Oxygen Species(ROS) and cytotoxicity. The range of MEK concentration detected in the area induced ROS generation affecting the oxidation state with a little effects on the viability of the cells.

• 농촌의학ㆍ지역보건
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• 제20권1호
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• pp.15-23
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• 1995

As compared before, agricultural machines are used more commonly instead of animal or manpower in rural areas and the injuries due to those are common. This study was conducted by questionnaire method in order to find out the current status of injuries due to agricultural machines for farmers who was selected from three Gun's in Kyeongsangnam Province. The study subjects were 385 persons in all(210 male persons, 175 female reasons) and the study period was from July through September 1993. The results were as follows; 1. The injury rate due to agricultural machines was high in male(p<0.05), and it was higher in younger age group and higher educated group. 2. The injury occurred high in summer and autumn seasons(77.6%), in the afternoon(60.6%), and during harvest(35.2%). 3. The major injuries were contusion, fracture, amputation in order and the injured sites were arms, legs, and chest in order. 31.7% of the injured farmers had been admitted, and they were treated at hospitals, home, drugstores and health centers in order. 4. The casualty damage was highest by cultivators, and agricultural instruments, threshing machine were followed. Among traumatic injuries concerned with cultivators contusions were most common, and fractures, amputations were followed. In case of agricultural instruments bruises were most common, and incisions, contusion were followed. In case of threshing machines fractures were most common and contusion, bruise were followed.

• 한국안전학회지
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• 제31권1호
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• pp.105-110
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• 2016

Mountainous disasters such as landslides and debris flow are difficult to forecast. Debris flow in particular often flows along the valley until it reaches the road or residential area, causing casualties and huge damages. In this study, the researchers selected Seoraksan National Park area located at Inje County (Inje-gun), Gangwon Province-where many mountainous disasters occur due to localized torrential downpours-for the damage reduction and cause analysis of the area experiencing frequent mountainous disasters every year. Then, the researchers conducted the field study and constructed geospatial information data by GIS method to analyze the characteristics of the disaster-occurring area. Also, to extract more precise geographic parameters, the researchers scanned debris flow triggering area through terrestrial LiDAR and constructed 3D geographical data. LiDAR geographical data was then compared with the existing numerical map to evaluate its precision and made the comparative analysis with the geographic data before and after the disaster occurrence. In the future, it will be utilized as basic data for risk analysis of mountainous disaster or disaster reduction measures through a fine-grid topographical map.