• 제목/요약/키워드: Initial concentration

검색결과 3,416건 처리시간 0.028초

Optimization of cell growth and TAPS production by Pichia ciferrii mutant in batch culture

  • 오대일;홍성갑;유연우
    • 한국생물공학회:학술대회논문집
    • /
    • 한국생물공학회 2003년도 생물공학의 동향(XIII)
    • /
    • pp.273-277
    • /
    • 2003
  • Batch culture of mutant derived from Pichia ciferrii ATCC 14091 was investigated for producing the intracellular tetraacetylphytosphingosine (TAPS). Composite experimental design was used to optimize the composition of the culture medium for maximizing the productivity of TAPS. In this experiment, various culture parameters were investigated that were the effects of temperature, the initial culture pH, the carbon-to-nitrogen ratio, the concentration of trace elements, and the concentration of cofactors. The optimal temperature for cell growth and TAPS synthesis appeared to be %25^{\circ}C$. An initial pH value of 7.5 gave the best results. Under the best condition, the maximum TAPS concentration indicated 7.2 g/L and its productivity was 0.06 g/L-hr in a 2.5 L jar

  • PDF

고농도 에탄올 내성균 Acetobacter sp. FM-10을 이용한 초산 발효조건 검토 (Investigation of the Condition of Acetic Acid Fermentation with High Concentration Ethanol Resistant Acetobacter sp. FM-10)

  • 박권삼;이명숙;목종수;장동석
    • 한국식품영양과학회지
    • /
    • 제23권5호
    • /
    • pp.845-848
    • /
    • 1994
  • The fermenting conditions for acetic acid production with Acetobacter sp. FM-10 which could grow in the medium containing 10% ehtanol were investigated. Initial concentration of acetic acid in broth medium affected greatly to the fermentation speed. For example , the acetic acid production increased proportionally by the increasing of initial concentration was higher that 1.0%. When the cultivation was started with broth medium containing 5% ethanol, the additional adding ethanol during the fermentation was not significantly increased the acidity of the medium. The acidity of the medium containing 10% ethanol was reached to 8.3% after shaking than static cultivation by about 10 days with 150 rpm shaking speed. Acetic acid production with shaking cultivation was faster the static cultivation by abot 10 days under the same condition except shaking. In acetic acid fermentation with the batch style fermentor , the optimum fermentation condition was 700 rpm of agitation speed and 5L/min air flow rate in 3L culture medium .

  • PDF

TiO2/UV공정을 이용한 수중 MTBE의 광분해 특성 (Characteristic of Photodegradation of MTBE Using TiO2/UV Process)

  • 류성필;김성수;오윤근
    • 한국환경과학회지
    • /
    • 제13권3호
    • /
    • pp.289-295
    • /
    • 2004
  • The objective of this study is to delineate removal efficiency of the MTBE in solution by $TiO_2$ photocatalytic degradation as a function of the following different experimental conditions: Initial concentration of MTBE, air flow rate in solution, $H_2O_2$ dosage and pH of the solution. Photodegradation rate was increased with decreasing initial concentration of MTBE. The removal efficiency was 82% after 180 min in the case of MTBE concentration of 100 mg/L but 100% after 180 min in the case of 20 mg/L. Removal efficiency was increased with increasing pH, $H_2O_2$ dosage and air flow rate in solution.

응집성 Saccharomyces cerevisiae CA-1에 의한 에탄올 연속발효 (Continuous Ethanol Fermentation in Air-lift Reactor by Flocculent Saccharomyces cerevisiae CA-1)

  • 이용범;심상국;한면수;정동효
    • 한국미생물·생명공학회지
    • /
    • 제23권6호
    • /
    • pp.717-722
    • /
    • 1995
  • Using a flocculating Saccharomyes cerevisiae CA-1, an air-lift reactor equipped with a modified settler was used for ethanol fermentation. The effects of conditions such as aeration rate, initial glucose concentration, and dilution rate were studied using the air-lift reactor. In batch fermentation, optimum aeration rate was 0.5 vvm. In continuous fermentation, aeration rate and initial pH were fixed 0.5 vvm and 4.5, substrate concentration and dillution rate were changed 10-15% and 0.1-1.3. The maximum ethanol productivity was shown to be 20.4 g/l$\cdot $h in 10% glucose and 0.7 h$^{-1}$ dilution rate., and optimum operation condition considering the ethanol productivity and glucose utilization ratio was 0.5 h$^{-1}$ dilution rate in 10% glucose concentration.

  • PDF

1-Amino-4-(3'-Acetaminoanilino) Anthraquinone-2-Sulfonic Acid 합성의 전화율에 미치는 반응조건의 영향 (The Effect of Reaction Conditions on the Conversion of Synthesis of 1-Amino-4-(3'-Acetaminoanilino) Anthraquinone-2-Sulfonic Acid)

  • 천재기;구수진
    • 한국인쇄학회지
    • /
    • 제16권3호
    • /
    • pp.133-146
    • /
    • 1998
  • The conversion of 1-Amino-4-(3'-Acetaminoanilino) Anthraquinone-2-Sulfonic Acid prepared by the Ullmann condensation have been investigated as a function of the reaction conditions such as catalysts, initial concentration of reactants, reaction temperature, pH, condensation agents and neutralizing agents. The experimental results showed that the Cu(I)-type catalysts had higher activity than the Cu(II)-type catalysts in this study. The conversion increased with increasing initial concentration of reactants but decreased at above critical concentration owing to agglomeration of reactants. It was also observed that the maximum conversion obtained at 7$0^{\circ}C$ and pH=7~8.

  • PDF

Cellulase Production in Fed-Batch Culture by Trichoderma reesei Rut C30

  • Yu, Xiao-Bin;Yun, Hyun-Shik;Koo, Yoon-Mo
    • Journal of Microbiology and Biotechnology
    • /
    • 제9권1호
    • /
    • pp.44-49
    • /
    • 1999
  • Cellulase production by fed-batch cultivation of Trichoderma reesei Rut C30 with various initial concentrations of Solka Floc in 1 % wheat bran-containing medium was investigated. The cellulase activity and productivity increased with initial Solka Floc concentration up to 5%. When a total Solka Floc concentration of 90 g/l was used for cellulase production, CMC (carboxymethyl cellulose) and FP (filter paper) activities, productivity, and yield were 359.7 U/ml, 30.61 U/ml, 161 FPU $L^{-1}$ $h^{-1}$, and 340 FPU $g^{-1}$, respectively. It was important to maintain a high cell concentration during cellulase production to obtain high cellulase activity and productivity. Cellulase powder was prepared by ammonium sulfate precipitation: FP activity was 396.7 U/g and CMC activity was 6481 U/g.

  • PDF

High Molecular Weight Poly(L-lactide) Synthesized in Supercritical Fluids

  • Kim, Soo-Hyun
    • 한국고분자학회:학술대회논문집
    • /
    • 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
    • /
    • pp.210-211
    • /
    • 2006
  • A series of L-LA polymerizations initiated by $Sn(Oct)_{2}\;([LA]_{0}/[Sn]_{0}=200)$ were carried out in scR22 at $130^{\circ}C$ and 300 bar, where $[LA]_{0}$ is the initial L-lactide concentration and $[Sn]_{0}$ is the initial $Sn(Oct)_{2}$ concentration. The reaction time dependences of monomer conversion and PLLA MW improved. The monomer conversion and PLLA MW increased with increasing reaction time. The effect of temperature on monomer conversion and PLLA MW was investigated in a series of polymerizations conducted at temperatures ranging from 90 to $150^{\circ}C$ and at a constant pressure of 200 bar. In all of these experiments, the ratio of monomer to R22 concentration was held constant at 12.4 wt.-%. Increasing the reaction temperature from 90 to $130^{\circ}C$ resulted in increased monomer conversion from 11.5 to 72.2 %.

  • PDF

Ferrate(VI)를 이용한 퍼클로로에틸렌의 분해특성 연구 (Degradation of perchloroethylene by ferrate(VI))

  • 김일규
    • 상하수도학회지
    • /
    • 제29권1호
    • /
    • pp.39-46
    • /
    • 2015
  • The degradation characteristics of perchloroethylene by ferrate(VI) oxidation have been studied. The degradation efficiency of perchloroethylene in aqueous solution was investigated at various values of ferrate(VI) dosage, pH, initial concentration of perchloroethylene and aqueous solution temperature. GC-ECD has been used to analyze the changes of perchloroethylene concentration. The optimum conditions of perchloroethylene degradation were obtained at pH 7.0 and $25^{\circ}C$ of aqueous solution temperature. Also, the experimental results showed that perchloroethylene removal efficiency increased with the decrease of initial concentration of perchloroethylene. Lastly intermediate products were identified by GC-MS techniques. Trichloroethylene and chloroform were identified as reaction intermediates.

활성탄에 의한 Tartrazine의 흡착 특성 (Adsorption Charateristics of Tartrazine by Activated Carbon)

  • 윤성욱;이종집
    • 한국물환경학회지
    • /
    • 제25권4호
    • /
    • pp.568-572
    • /
    • 2009
  • The adsorption characteristics of tartrazine by granular activated carbon were experimentaly investigated in the batch adsorber and the packed column. The adsorptivity of activated carbon for tartrazine was largely improved by pH control, and 98 percent of initial concentration could be removed at pH 3. It was estabilished that the adsorption equilibrium of tartrazine on granular activated carbon was sucessfully fitted by Freundlich isotherm equation in the concentration range from 50 mg/L to 1,000 mg/L. The characteristics of breakthrough curve of activated carbon packed column, which depend on the design variables such as initial concentration, bed height, and flow rate, were studied.

A Kinetic Study on the Photocatalytic Degradation of Gas-Phase VOCs Using TiO$_2$ photocatalyst

  • Kim, Sang-Bum;Jo, Young-Min;Hong, Sung-Chang
    • Journal of Korean Society for Atmospheric Environment
    • /
    • 제17권E3호
    • /
    • pp.117-124
    • /
    • 2001
  • The present paper examined the kinetics of photocatalytic degradation of volatile organic compounds (VOCs) including gaseous trichloroethylene (TCE) and acetone. In this study, we examined the effects of the initial concentration of VOCs and the light intensity of ultra-violet (UV). A batch photo-reactor was specifically designed for this work. The photocatalytic degradation rate increased with the initial concentration of VOCs but remained almost constant beyond a certain concentration. It matched well with the Langmuir-Hinshelwood (L-H) kinetic model. When the effect of light intensity was concerned, it was found that photocatalytic degradation occurs in two regimes with respect to light intensity.

  • PDF