• Korean Journal of Microbiology
• /
• v.36 no.2
• /
• pp.155-160
• /
• 2000

Pseudomnas sp. HJ-2 is capable of producing a rubber-elastic polyhydroxyalkanoate (PHA) consisting of 3- hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyheptanoate (3HHp) from heptanoic acid as the sole carbon source. The polyester produced was a blend of poly(3HB-co-3HV) and poly(3HHp). Although the mixing of poly(3HHp) fraction to poly(3HB-co-3HV) resulted in a decrease of modulus, the sole fraction of poly(3HB-co-3HV) with a high molar fraction of 3HV was shown to be an elastomer with the maximum percent strain of 740%. The biomass yield and the PHA synthesis were relatively high when the initial heptanoic acid concentration was 40 mM, and were significantly decreased when the substrate concentration exceeded 50 mM. The accumulation of PHA was stimulated by deficiency of nitrogen and phosphorus in the medium. The PHA contents and its monomeric compositions were greatly affected by pH and oxygen transfer rate. At pH 7.5, poly(3HB-~0.38% 3HV) was produced from heptanoic acid and a mixture of 95% 3HHp and 5% 3HV was produced at pH 8.0. Increased conten1 of 3HHp in the polyesters with lhe increasing oxygen transfer rate by agitation speed a1 a fixed aeration rate was observed.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.15 no.3
• /
• pp.201-209
• /
• 2015

The deformed degree of car body varies largely with the collision part from side collision of car-to-car. In case of deformation of car body caused by collision, the movement is different as speed energy changes to strain energy. Generally, in the analysis of traffic accident, the movement of car after the collision is analyzed by law of conservation of motion and the error of energy absorption rate along the deformation of car body can be calibrated by inputting coefficient of restitution, but it is current situation that coefficient of restitution applied by referring to the research results of forward collision and backward collision because the research results of side collision is rare. Vehicle model of finite element method applied by structure of car body and materials of each component was analyzed by explicit finite element method, and coefficient of restitution and collision detection time along contact part of side collision was drawn by analyzing the results. Analysis result acquired through the law of conservation momentum by applying finally-computed coefficient of restitution and crash detection time compared to collision result of actual vehicle. As a result, the reliability of analysis was higher than the existing analysis method were acquired when applying the drawn initial input value that used finite element method analysis model.

• Applied Biological Chemistry
• /
• v.38 no.6
• /
• pp.507-511
• /
• 1995

In an attempt to improve the productivity of ${\beta}-galactosidase$ from Bacillus sp. A1, which was isolated from soil and has remarkably higher transgalactosylation activity than lactose hydrolysis activity, a chemical mutation procedure using N-methyl-N'-nitro-N-nitrosoguanidine followed by selection was conducted. The final selection, designated as Bacillus sp. A4442, turned out to show a substantially increased enzyme productivity. Catabolite repression by glucose and lactose requirement as an inducer for the enzyme biosynthesis, which were shown in the parent strain, was markedly diminished; instead it was found out that galactose acts as another inducer. Because pH of medium, one of the most important factors for cell growth as well as enzyme production, is closely related with the sugar concentration during culture, it was kept in the optimum range of $6.5{\sim}7.5$; for this the initial glucose concentration was adjusted to be 0.5% which was thereafter maintained by the controlled pumping-in of lactose using the pH-stat technique. By doing so, we were able to increase the productivity of ${\beta}-galactosidase$ with high transgalactosylation activity up to $44\;unit/m{\ell}-broth$.

• Applied Biological Chemistry
• /
• v.51 no.1
• /
• pp.1-5
• /
• 2008

Salt accumulation in soils of greenhouse due to the massive application of nitrogen fertilizers causes salt stress on the various crops, a serious problem in domestic agriculture. Since the majority of the salinity is nitrate, the excess nitrate should be removed; therefore, a bacterial strain having high capacity of nitrate uptake and identified as Enterobacter amnigenus GG0461 was isolated from the soils of greenhouse. Optimum conditions for the bacterial growth and nitrate uptake were investigated. GG0461 was able to grow without nitrate; however, nitrate facilitated the growth. The rate of nitrate uptake increased at alkaline pH and both growth and nitrate uptake were maximal at pH 8-9. When the initial pH of culture medium was increased to pH 8 or 9, it was decreased to neutral upon bacterial growth and nitrate uptake. These results imply that the major factor mediating bacterial nitrate uptake is a nitrate/proton antiporter. The fact was supported by the effect of nitrate addition in the absence of nitrate, since the addition of nitrate greatly increased the nitrate uptake and rapidly decreased pH of media.

• Journal of Life Science
• /
• v.12 no.1
• /
• pp.77-86
• /
• 2002

Chitin, a $\beta$-1,4 polymer of N-acetyl-D-glucosamine, is one of the most abundant organic compounds in nature. Chitinase (EC 3.2.1.14) is an enzyme that degrades chitin to chito-oligosaccharides, diacetyl rhitobiose and N-acetyl-D-glucosamine. An extracellular chitinase-producing bacterial strain was isolated from soil and named to as Bacillus subtilis JK-56. Optimum culture condition of B. subtilis JK-56 for the production of chitinase was 1% chitin, 0.5% polypepton, 0.1% KCl, 0.05% MnS $O_4$.4$H_2O$, 37$^{\circ}C$, initial pH 7.0 and 40 hour culture time. When B. subtilis JK-56 was grown in the optimum medium, one major active band and two minor active bands were detected by native-PAGE and active staining of the gel. Among them, the major band was purified from the culture supernatant by 70% ammonium sulfate precipitation and native-PAGE with BIO-RAD Model 491 Prep-Cell and named as Chi-56A. Its molecular weight was estimated to be 53kDa monomer and the isoelectric point (pI) was pH 4.3. The pH and temperature for the optimum activity of Chi-56A were pH 6.0 and $65^{\circ}C$, respectively. Chi-56A was stable up to $65^{\circ}C$ and in alkaline region. Its $K_{m}$ value for colloidal chitin was 17.33g/L. HPLC analysis of the reaction products confirmed that Chi-56A was an exo type chitinase.e.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.8 no.1
• /
• pp.195-202
• /
• 2004

The shell due to the effect of initial normal pressures on the shell surface was based on the assumption that the directions of the pressures are always normal to the undeformed shell surface, and that the change in the surface area of the shell is negligible. But the fact that the pressure are always normal to the deforming surface leads "follower force". The follower effect in the analysis can significantly alter the solution for natural frequency and buckling load as compared to the case when the direction of the pressures are assumed to be normal to the uniform shell surface. The expression for the part of strain energy contribution from normal pressure due to the effect of follower force was derived and added to the element stiffness matrix of axisymmetric shell. In the case of increasing external pressure, the natural frequencies decrease until one of them reaches zero. Theoretically the smallest applied load that reduces the frequency of any mode to zero, will have same magnitude as that of the buckling load. In order to determine the bucking load of the shell a few sets of frequencies are computed and the results considering the follower effects are well with the exact solution while the case without that are quite different. But in case of hemispherical dome, there are little difference in buckling pressure between with and without the effect of follower force.

• Food Engineering Progress
• /
• v.14 no.1
• /
• pp.65-74
• /
• 2010

Culture conditions of L. plantarum TJ-LP-002, the garlic resistant strain isolated from pakimchi (green onion kimchi), were investigated for the use of feed additives. Acetic acid, citric acid, lactic acid, and tartaric acid were detected in the culture supernatant, and especially the concentrations of lactic acid and acetic acid significantly increased during cultivation. The antimicrobial activity of L. plantarum TJ-LP-002 was not affected by proteases, calatase or cellulase, which showed that the antimicrobial activity might be due to the production of acids rather than proteinaceous antimicrobial substances. L. plantarum TJ-LP-002 was resistant to neomycin sulfate, spectinomycin dihydrochloride, and lincomycin hydrochloride, sensitive to streptomycin sulfate, and intermediate resistant to ampicillin trihydrate, chloramphenicol, erythromycin, tetracycline hydrochloride, and kanamycin sulfate. The optimum initial pH of medium, fermentation temperature and time for the cell growth and antibacterial activity were pH 7.0, 30${^{\circ}C}$ and 24hr, respectively. The optimal composition of culture medium for the cell growth and antimicrobial activity was 3%(w/v) glucose as a carbon source, 3%(w/v) yeast extract as a nitrogen source, and manganese sulfate and ammonium citrate as inorganic salts. The combinatorial supplementation of these inorganic salts, rather than sole addition as an inorganic salt, resulted in better antibacterial activity.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.23 no.3
• /
• pp.143-149
• /
• 2019

In this study, a U-shape composite beam was developed to be effectively used for a steel parking lot which is 8m or lower in height. When the U-shape composite beam was applied to a steel parking lot, essential considerations were story-height and long-span. In addition, due to the mixed structural system with reinforced concrete and steel material, the U-shape composite beam needed to have a structural integrity and reliable performance over demand capacity. The main objective of this study was to investigate the performance of the structure consisting of the reinforced concrete (RC) slab and U-shape beam. A U-shape composite beam generally used at a parking lot served as a control specimen. Four specimens were tested under four-point bending. To calculate theoretical values, strain gauges were attached to rebar, steel plate, and concrete surface in the middle of the specimens. As the results, initial yielding strength of the control specimen occurred at the bottom of the U-shaped steel. After yielding, the specimen reached the maximum strength and the RC slab concrete was finally failed by concrete crush due to compressive stress. The structural performance such as flexural strength and ductility of the specimen with the increased beam depth was significantly improved in comparison with the control specimen. Furthermore, the design of the U-shape composite beam with the consideration of flexural strength and ductility was effective since the structural performance by a negative loading was relatively decreased but the ductile behavior was evidently improved.

• The Korean Journal of Mycology
• /
• v.47 no.1
• /
• pp.1-12
• /
• 2019

This study was conducted to investigate the optimum culture conditions, for mass production of Tremella fuciformis in M9 basic medium. The strain KMCC04674, used in this study was identified T. fuciformis by internal transcribed spacer (ITS). To define the optimum conditions for the mass production of T. fuciformis, we investigated the effects of different culture conditions and various nutrient sources on the fungal growth. The optimum initial pH and temperature for the fungal growth were 5.0 and $25^{\circ}C$, respectively. The optimal composition of the growth medium was 4.0% mannitol, 3.0% $NH_4H_2PO_4$, 1.0% malt extract, 1.0% glutamic acid, 5mM $CaCl_2$, and 0.5% glucanic acid.

• Korean Journal of Microbiology
• /
• v.54 no.4
• /
• pp.343-353
• /
• 2018

Our previous research demonstrated the essential role of the xenB gene in stress response to RDX by using Pseudomonas sp. HK-6 xenB knockout. We have extended this work to examine the cellular responses and altered proteomic profiles of the HK-6 xenA knockout mutant under RDX stress. The xenA mutant degraded RDX about 2-fold more slowly and its growth and survival rates were several-fold lower than the wild-type HK-6 strain. SEM revealed more severe morphological damages on the surface of the xenA mutant cells under RDX stress. The wild-type cells expressed proportionally-increased two stress shock proteins, DnaK and GroEL from the initial incubation time point or the relatively low RDX concentrations, but slightly less expressed at prolonged incubation period or higher RDX. However the xenA mutant did not produced DnaK and GroEL as RDX concentrations were gradually increased. The wild-type cells well maintained transcription levels of dnaA and groEL under increased RDX stress while those in the xenA mutant were decreased and eventually disappeared. The altered proteome profiles of xenA mutant cells under RDX stress also observed so that the 27 down-regulated plus the 3 up-regulated expression proteins were detected in 2-DE PAGE. These all results indicated that the intact xenA gene is necessary for maintaining cell integrity under the xenobiotic stress as well as performing an efficient RDX degradation process.