• Mycobiology
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• 제44권3호
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• pp.171-179
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• 2016

In the screening of marine mangrove derived fungi for lovastatin productivity, endophytic Aspergillus luchuensis MERV10 exhibited the highest lovastatin productivity (9.5 mg/gds) in solid state fermentation (SSF) using rice bran. Aspergillus luchuensis MERV10 was used as the parental strain in which to induce genetic variabilities after application of different mixtures as well as doses of mutagens followed by three successive rounds of genome shuffling. Four potent mutants, UN6, UN28, NE11, and NE23, with lovastatin productivity equal to 2.0-, 2.11-, 1.95-, and 2.11-fold higher than the parental strain, respectively, were applied for three rounds of genome shuffling as the initial mutants. Four hereditarily stable recombinants (F3/3, F3/7, F3/9, and F3/13) were obtained with lovastatin productivity equal to 50.8, 57.0, 49.7, and 51.0 mg/gds, respectively. Recombinant strain F3/7 yielded 57.0 mg/gds of lovastatin, which is 6-fold and 2.85-fold higher, respectively, than the initial parental strain and the highest mutants UN28 and NE23. It was therefore selected for the optimization of lovastatin production through improvement of SSF parameters. Lovastatin productivity was increased 32-fold through strain improvement methods, including mutations and three successive rounds of genome shuffling followed by optimizing SSF factors.

• 대한전기학회논문지
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• 제38권12호
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• pp.995-1006
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• 1989

로보트 매니퓰레이터가 컨베이어 상의 파트를 추적 하면서 작업을 수행하는 것은 작업 효율의 증가에 크게 기여한다. 본 논문은 컨베이어 상의 파트를 추적하기 위한 로보트 매니퓰레이터의 동작계획 알고리즘을 제안한다. 로보트 매니퓰레이터의 추적 동작은 밸트의 속도, 추적 시작 시간에서의 파트와 로보트 핸드의 위체이 의하여 결정된다. 이때 토크 및 관절 속도, 관절 가속도, 관절 저크의 제한범위가 모두 고려되며, 최소의 정착시간을 갖는 추적 궤적이 생성된다. 추적 해는 linearr quadratic tracking문제로 접근하여 구한다. 로보트매니퓰레이터의 동적 방정식은 파라메터의 함수 성능 지수를 최소화 하는 동적 방정식의 해는 동적 계획법에 의하여 구한다. ${\mu}-VAX_@$에 의한 시뮬레이션 결과를 제시한다.

• Structural Monitoring and Maintenance
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• 제3권2호
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• pp.115-127
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• 2016

It is a challenging problem of assessing the location and extent of structural damages with vibration measurements. In this paper, an improved Extended Kalman filter (EKF) with Tikhonov regularization is proposed to identify structural damages. The state vector of EKF consists of the initial values of modal coordinates and damage parameters of structural elements, therefore the recursive formulas of EKF are simplified and modal truncation technique can be used to reduce the dimension of the state vector. Then Tikhonov regularization is introduced into EKF to restrain the effect of the measurement noise for improving the solution of ill-posed inverse problems. Numerical simulations of a seven-story shear-beam structure and a simply-supported beam show that the proposed method has good robustness and can identify the single or multiple damages accurately with the unknown initial structural state.

• 한국해양공학회지
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• 제22권6호
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• pp.52-57
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• 2008

The short-term high temperature creep rupture behavior of Ni-based Alloy718 steel was investigated at the elevated temperatures range of 550 to $700^{\circ}C$ under constant stress conditions. The creep rupture characteristics such as creep stress, rupture time, steady state creep rate, and initial strain were evaluated. Creep stress has a quantitative correlation between creep rupture tim and steady state creep rate. The stress exponents (n, m) of the experimental data at 550, 600, 650 and $700^{\circ}C$ were derived as 33.5, -24.9, 26.1, -21.2, 16.8, -12.8 and 10, -8.2, respectively. The stress exponent decreased with increasing creep temperature. The creep lift prediction was derived by the Larson-Miller parameter (LMP) method and the resultant equation was obtained as follows: T($logt_r$+20)=-0.00252 ${\sigma}^2$-1.377${\sigma}$+-22718.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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• pp.357-357
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• 2000

In this study, we consider a multi-input multi-output styrene polymerization reactor system for which the monomer conversion and the weight average molecular weight are controlled by manipulating the jacket inlet temperature and the feed flow rate. The reactor system is identified by using a linear subspace identification method and then the output feedback model predictive controller is constructed on the basis of the identified model. Here we use the Best Linear Unbiased Estimation (BLUE) filter as a stochastic estimator instead of the Kalman filter. The BLUE filter observes the state successfully without any a priori information of initial states. In contrast to the Kalman filter, the BLUE filter eliminates the offset by observing the state of the augmented system regardless of a priori information of the initial state for an integral white noise augmented system. A BLUE filter has a finite impulse response (FIR) structure which utilizes finite measurements and inputs on the most recent time interval [i-N, i] in order to avoid long processing times.

• 한국항공우주학회지
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• 제39권8호
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• pp.796-803
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• 2011

정지 상태에서 관성항법장치를 구성하는 가속도계 및 자이로 측정치를 이용하여 초기 자세를 구하는 것을 초기정렬 혹은 자가정렬이라 한다. 초기정렬의 정밀도는 관성항법장치에 탑재되는 관성센서의 성능에 의하여 결정되며 수평축 자세는 수평축 가속도계, 수직축 자세는 E축 자이로 성능에 의해 결정된다. 그러므로 관성센서에서 발생된 불확실한 오차는 초기정렬의 정밀도를 저하시키는 주요원인이 된다. 논 논문에서는 관성센서의 불확실한 오차 중에서 관성항법장치에 전원이 인가되어 온도가 안정화 되는 상태에서의 관성센서 오차가 초기정렬 성능에 어떠한 영향을 미치는 가를 이론적으로 분석하고 시뮬레이션을 통하여 검증한 결과를 제시한다.

• Journal of Applied Biological Chemistry
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• 제59권4호
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• pp.289-298
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• 2016

FK506 hyper-yielding mutant, called the TCM8594 strain, was made from Streptomyces tsukubaensis NRRL 18488 by mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine, ultraviolet irradiation, and FK506 sequential resistance selection. FK506 production by the TCM8594 strain improved 45.1-fold ($505.4{\mu}g/mL$) compared to that of S. tsukubaensis NRRL 18488 ($11.2{\mu}g/mL$). Among the five substrates, wheat bran was selected as the best solid substrate to produce optimum quantities of FK506 ($382.7{\mu}g/g$ substrate) under solid-state fermentation, and the process parameters affecting FK506 production were optimized. Maximum FK506 yield ($897.4{\mu}g/g$ substrate) was achieved by optimizing process parameters, such as wheat bran with 5 % (w/w) dextrin and yeast extract as additional nutrients, 70 % (v/w) initial solid substrate moisture content, initial medium pH of 7.2, $30^{\circ}C$ incubation temperature, inoculum level that was 10 % (v/w) of the cell mass equivalent, and a 10 day incubation. The results showed an overall 234 % increase in FK506 production after optimizing the process parameters.

• Nuclear Engineering and Technology
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• 제34권4호
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• pp.382-395
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• 2002

An experimental study on transient critical heat flux (CHF) under flow coastdown has been performed for the water flow in a non-uniformly heated vertical annulus under low flow and a wide range of pressure conditions. The objectives of this study are to systematically investigate the effect of the flow transient on the CHF and to compare the transient CHF with steady-state CHF The transient CHF experiments have been performed for three kinds of flow transient modes based on the coastdown data of a nuclear power plant reactor coolant pump. At the same inlet subcooling, system pressure and heat flux, the effect of the initial mass flux on the critical mass flux can be negligible. However, the effect of the initial mass flux on the time-to- CHF becomes large as the heat flux decreases. The critical mass flux has the largest value for slow flow reduction rate. There is a pressure effect on the ratio of the transient CHF data to steady-state CHF data. Except under low system pressure conditions, the flow transient CHF was revealed to be conservative compared with the steady-state CHF data. Bowling CHF correlation and thermal hydraulic system code MARS show promising results for the prediction of CHF occurrence .

• Structural Engineering and Mechanics
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• 제71권2호
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• pp.109-118
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• 2019

This paper presents the numerical simulation of membrane structure under impact load. Firstly, the numerical simulation model is validated by comparing with the test in Hao's research. Then, the effects of the shape of the projectile, the membrane prestress and the initial impact speed, are investigated for studying the dynamic response and failure mechanism, based on the membrane displacement, projectile acceleration and kinetic energy. Finally, the results show that the initial speed and the punch shape are related with the loss of kinetic energy of projectiles. Meanwhile, the membrane prestress is an important factor that affects the energy dissipation capacity and the impact resistance of membrane structures.

• 한국전기화학회:학술대회논문집
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• 한국전기화학회 2001년도 추계총회 및 학술발표회
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• pp.40-40
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• 2001