• The Korean Journal of Zoology
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• v.39 no.4
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• pp.378-386
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• 1996

We have developed a couple of new luciferase reporter plasmida with very low background reporter actlvltlea. One can be used to measure the promoter strength, after insertion of some promoter fragment into the reporter plasmid, and the other, with very low basal promoter actlvltlea, allis In studying eukaryotic transcriptional regulators. The latter reporter plasmid contains such cli elements as a 17 nucleotide long inftlator, Spl.blndIng sftes, GAL4 binding sltea, and bInding sitea for a certain Drosophila homeodomain proteins. In an attempt to construct an improved reporter plaimid by fadlltating transcriptional termination and minimizing any interference by cryptic promoters which may be preaent in the reporter pleamld DNA, we have inserted transsrlptional termination-related signals, a three tandem repeat of SV4O polyadenylatlon signal (AAA) and the putative transcrtptional termination signal (UMS) of the mouse c-mos gene, Into just upstream of the initIator, and the promoter actlvitiea were measured by a transIent expression assay employing the Drosophila Schneider line 2 cells. As expected, the basal promoter activitIes decreased maximally when both transcription termination related elements were inserted. Moreover, the reporter plasmld with the two elements allowed more sensitive measurement of transcriptional activation than the reporter piasmid without them. Theae reporter plasmids can be used for studying transcriptional regulators of higher organisms Including mammals as well as Droiophlla melanogaiter.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2007.11a
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• pp.252-255
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• 2007

Recently military industrial company, utilizing company funded R&D and goverment and industry contracts, has developed ACTS/DACS technology. This technology can be utilized to rapidly steer "smart" bullets, "smart" rounds, tactical missile, cruise missile and kill vehicles for both endo- and exoatmospheric applications. The ACTS/DACS typically consists of a Smart Bus Controller(SCB), a proprietary network firing bus, Smart Pyrotechnic Devices(SPD), rocket motors, and a structure. The SCB communicates with the SPDs over the propretary network firing bus. Each rocket motor contains an SPD which provides rocket motor ignition. Firing energy is stored locally in the SPD so surge currents do not occur in the system as rocket motors are fired. This approach allows multiple, truly simultaneous firings without the need for large, dedicated batteries. Each SPD also functions as a network tranceiver and high reliability fir set all in the space of a single-sided 10 millimeter diameter circuit. The present work develops a new means for igniting explosive materials. The volume of semiconductor bridge (SCB) is over 30 times smaller than a conventional hot wire. We believe that the present work has a potential for development of a new igniter such as smart pyrotechnic device.