• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1250-1257
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• 2005

Two groups of exopolysaccharides (designated as Fr-I EPS and Fr-II EPS) were isolated from the culture filtrate of new fungal strain Trichoderma erinaceum DG-312 by Sepharose CL-6B chromatography. The structures of the exopolysaccharides were investigated using gas chromatography (GC), Fourier transform-infrared (FT-IR) spectroscopy, GCMS analysis, and NMR. GC analysis indicated that Fr-I EPS was composed of mainly mannose ($78.9\%$) and galactose ($21.1\%$), whereas Fr-II EPS contained mannose ($68.4\%$), galactose ($26.2\%$), and glucose ($5.4\%$). In the anomeric region ($950-700cm_{-1}$) of the FT-IR spectrum, both EPSs exhibited obvious characteristic absorption of $810\;cm_{-1}$, indicating the existence of mannose. The spectra of $\alpha-and\;\beta$-configurations were assigned at 880 and $914\;cm_{-1}$, respectively. The results of GC-MS analyses confirmed that both EPSs were complex heteropolysaccharides with a ($1{\rightarrow}3$)-linked mannan backbone. The C-1 region that appeared in the $^{13}C-NMR$ spectra of these EPSs indicated a typical anomeric carbon signal. The Fr-I EPS showed two anomeric carbon signals at 102.6 and 99.6 ppm, whereas the Fr-II EPS displayed four anomeric carbon signals at 102.5, 99.6, 98.5, and 94.3 ppm. The molecular characteristics of the EPSs were further investigated using a size exclusion chromatography/multi-angle laser light scattering (SEC/MALLS) system. The SEC/MALLS system revealed that the average molar masses of the EPSs were $6.592{\times}10^{4}$ (Fr-I EPS) and $1.920{\times}10^{4}$ (Fr-II EPS) g/mol, and the molecular conformation of both EPSs in aqueous solution was random coils.

• Journal of Biosystems Engineering
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• 제32권5호
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• pp.339-347
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• 2007

A hexapod walking robot had been developed for gathering information in the field. The developed robot was $260{\times}260{\times}130$ ($W{\times}L{\times}H$, mm) in size and 14.7 N in weight. The legs had nineteen degrees of freedom. A leg has three rotational joints actuated by small servomotors. Two servomotors placed at ankle and knee played the roles of vertical joint for up and down motions of the leg and the other one placed at coxa played the role of horizontal joint for forward and backward motions. In addition, a servomotor placed at thorax between the front legs and the middle legs played the role of vertical joint for pumping the two front legs to climb stair or inclination. Walking motion of the robot was executed by tripod gait. The robot was controlled by manual remote-controller communicated by an infrared ray. Two controllers were equipped to control the walking of the robot. The sub-controller using PIC microcomputer (Microchips, PIC16F84A) received the 16 bit command signal from the manual remote controller, decoded it to 8bit and transmitted it to the main microcomputer (RENESAS, SH2/7045), which controlled the 19 servomotors using the PWM command signals. Walking speeds were controlled by adjusting the period of command cycle and the stride. Forward walking speed were within 100 cm/min to 300 cm/min. However, experimental walking speed had the error of 4-40 cm/min to compare with the theoretical one, because of slippage of the leg and the circular arc motion of servomotor of coxa.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume I
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• pp.90-93
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• 2006

The first Geostationary Ocean Color Imager (GOCI) onboard its Communication Ocean and Meteorological Satellite (COMS) is scheduled for launch in 2008. GOCI includes the eight visible-to-near-infrared (NIR) bands, 0.5km pixel resolution, and a coverage region of 2500 ${\times}$ 2500km centered at 36N and 130E. GOCI has had the scope of its objectives broadened to understand the role of the oceans and ocean productivity in the climate system, biogeochemical variables, geological and biological response to physical dynamics and to detect and monitor toxic algal blooms of notable extension through observations of ocean color. The special feature with GOCI is that like MODIS, MERIS and GLI, it will include the band triplets 660-680-745 for the measurements of sun-induced chlorophyll-a fluorescence signal from the ocean. The GOCI will provide SeaWiFS quality observations with frequencies of image acquisition 8 times during daytime and 2 times during nighttime. With all the above features, GOCI is considered to be a remote sensing tool with great potential to contribute to better understanding of coastal oceanic ecosystem dynamics and processes by addressing environmental features in a multidisciplinary way. To achieve the objectives of the GOCI mission, we develop the GOCI Data Processing System (GDPS) which integrates all necessary basic and advanced techniques to process the GOCI data and deliver the desired biological and geophysical products to its user community. Several useful ocean parameters estimated by in-water and other optical algorithms included in the GDPS will be used for monitoring the ocean environment of Korea and neighbouring countries and input into the models for climate change prediction.

• 대한인간공학회지
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• 제31권3호
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• pp.427-435
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• 2012

The aim of this study is to investigate facial temperature changes induced by facial expression and emotional state in order to recognize a persons emotion using facial thermal images. Background: Facial thermal images have two advantages compared to visual images. Firstly, facial temperature measured by thermal camera does not depend on skin color, darkness, and lighting condition. Secondly, facial thermal images are changed not only by facial expression but also emotional state. To our knowledge, there is no study to concurrently investigate these two sources of facial temperature changes. Method: 231 students participated in the experiment. Four kinds of stimuli inducing anger, fear, boredom, and neutral were presented to participants and the facial temperatures were measured by an infrared camera. Each stimulus consisted of baseline and emotion period. Baseline period lasted during 1min and emotion period 1~3min. In the data analysis, the temperature differences between the baseline and emotion state were analyzed. Eyes, mouth, and glabella were selected for facial expression features, and forehead, nose, cheeks were selected for emotional state features. Results: The temperatures of eyes, mouth, glanella, forehead, and nose area were significantly decreased during the emotional experience and the changes were significantly different by the kind of emotion. The result of linear discriminant analysis for emotion recognition showed that the correct classification percentage in four emotions was 62.7% when using both facial expression features and emotional state features. The accuracy was slightly but significantly decreased at 56.7% when using only facial expression features, and the accuracy was 40.2% when using only emotional state features. Conclusion: Facial expression features are essential in emotion recognition, but emotion state features are also important to classify the emotion. Application: The results of this study can be applied to human-computer interaction system in the work places or the automobiles.

• 한국지능시스템학회논문지
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• 제26권5호
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• pp.361-367
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• 2016

기존 디지털 출력 방식의 PIR 센서를 이용한 침입감지 시스템은 사람이 아닌 다른 물체에 대한 침입 탐지 오류가 많았다. 본 논문은 이를 극복하기 위하여 아날로그 출력 방식의 PIR 센서 기반 침입 감지 시스템을 제안한다. 아날로그 방식 PIR 센서는 임계값을 기준으로 이진 출력값 대신, 일정 범위 내의 다양한 전압 준위로 출력값을 내보낸다. 아날로그 PIR 센서를 이용하여 획득된 신호의 샘플링된 신호값으로부터 FFT(Fast Fourier Transform) 또는 MFCC(Mel-frequency cepstrum codfficents)을 이용하여 신호의 주파수 성분을 추출하여, 인공 신경회로망(Artificial Neural Network)의 특징벡터로 사용된다. 다양한 인간의 움직임과 애완동물의 움직임에 대한 신호 패턴들을 학습한 인공 신경회로망을 통해서 침입상황에서 침입한 객체가 사람인지 애완동물인지 판별하게 된다.

• 한국정보통신학회논문지
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• 제19권8호
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• pp.1825-1830
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• 2015

일부 보행자의 경우 보행자 신호등이 적색임에도 불구하고 무단횡단을 하거나 차도에 내려서서 보행 신호를 기다리기도 한다. 이럴 경우 자칫하면 교통사고로 이어질 수 있다. 이에 적외선 센서를 이용하여 보행자가 차도로 진입할 경우 경고 안내 방송을 해주는 방식으로 채택해왔으나, 이 경우 교차로의 곡선형 횡단보도에는 설치가 어렵다. 본 논문에서는 카메라의 영상 검지 방식을 사용하여 보행자를 검지하는 방법에 대해 제안한다. 보행철주 상단에 설치된 카메라를 이용하여 이미지를 획득하고, 획득한 이미지를 이용하여 보행자 검지구간을 설정한다. 또한, 획득한 이미지에 대해 소벨( Sobel) 영상 처리 방식을 이용하여 변환하고 변환한 이미지를 다른 이미지와 비교하여 보행자 검지방법을 선택하였다. 이 영상 검지 방식을 사용하면 곡선형 횡단보도에서 보행자를 검지하는데 효과적이다.

• Journal of Astronomy and Space Sciences
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• 제14권2호
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• pp.259-268
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• 1997

천문대에서 개발 중에 있는 CCD를 사용한 극미 광 영상장비의 시험모델 개발과 현재까지의 결과를 논의하였다. 고도의 저 잡음 도와 안정성이 요구되는 천문관측용 극미 광 영상장비는 자외선에서 가시 광 및 근 적외선 영역에 이르기까지 매우 넓은 파장영역에서 관측을 수행할 수 있는 장비로서 여러 분야에 널리 사용되고 있다. 그러나 현재가지 국내에서는 이 장비의 핵심부분인 controller의 자체설계 기술이 확보되어 있지 않은 실정이다. 이 연구에서 개발된 시스템의 전자 부는 한 개의 EPLD(Erasable Programable Logic Device)에 구현된 controller와, 그 외에 analog driver, video processor로 이루어져 있으며 CCD의 암잡음을 줄이기 위한 방법으로 액체질소 냉각방식을 사용하고 있다. 전체적인 제어와 자료처리는 범용성을 고려하여 개인용 컴퓨터(PC)를 이용하였다. 액체질소 냉각용 cryostat와 CCD 소자를 제외한 대부분이 자체 설계제작 되어진 이 시스템으로 실험실과 소백산천문대에서 시험영상을 얻는데 성공하였다.

• 한국감성과학회:학술대회논문집
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• 한국감성과학회 2000년도 추계학술대회 논문집
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• pp.91-97
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• 2000

The conventional model did not take momentum conservation into consideration when the electron absorbs and emits the photons. II-ray provides momentum conservations on any directions of the entering photons, and also the electrons have radial momentum conservations and fully elastic bouncing between two atoms, in the new atom model. Conventional atom model must be criticized on the following four points. (1) Natural motions between positive and negative entities are not circular motions but linear going and returning ones, for examples sexual motion, tidal motion, day and night etc. Because the radius of hydrogen atom's electron orbit is the order of 10$^{-11}$ m and the radia of the nucleons in the nucleus are the order of 10$^{-l4}$m and then the converging n-gamma rays to the nucleus have so great circular momentum, the electron can not have a circular motion. We can say without doubt that any elementary mass particle can have only linear motion because of the n-rays' hindrances, near the nucleus. (2) Potential energy generation was neglected when electron changes its orbit from outer one to inner one. The h v is the kinetic energy of the photo-electron. The total energy difference between orbits comprises kinetic and potential energies. (3) The structure of the space must be taken into consideration because the properties of the electron do not change during the transition from outer orbit to inner one even though it produces photon. (4) Total energy conservation law applies to the energy flow between mind and matter because we daily experiences a interconnection between mind and body. An understanding of the mechanisms responsible for the control of normal proliferation and differentiation of the various cell types which make up the human body will undoubtedly allow a greater insight into the abnormal growth of cells, A large body of biochemical evidence was eventually used to generate a receptor model with an external ligand binding domain linked through a single trans-membrane domain to the cytoplasmic tyrosine kinase and autophosphory-lation domains. The ligand induced conformational change in the external domain generates either a push-pull or rotational signal which is transduced from the outside to the inside of cell.l.ell.

• 대한원격탐사학회지
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• 제31권4호
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• pp.281-291
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• 2015

Sun glint correction methods of hyperspectral data that have been developed so far have not considered the various situations and are often adequate for only certain conditions. Also there is an inaccurate assumption that the signal in NIR wavelength is zero. Therefore, this study attempts to analyze the NIR spectral properties of sun glint effect in coastal waters. For the analysis, CASI-1500 airborne hyperspectral data, bathymetry data and in-situ data obtained at coastal area near Sin-Cheon, Jeju Island, South Korea were used. The spectral characteristics of radiance and reflectance at the five NIR wavelengths (744 nm, 758 nm, 772 nm, 786 nm, and 801 nm) are analyzed by using various statistics, spatial and spectral variation of sun-glinted area under conditions of the bottom types of benthos, barren rocks and sand with similar water depth. Through the quantitative analysis, we found that the relation of water depth or bottom type with sun glint is relatively less which is a similar result with the previous studies. However the sun glint are distributed similarly with the patterns of the direction of wave propagation. It is confirmed that the areas with changed direction of wave propagation were not affected by the sun glint. The spatial and spectral variations of radiance and reflectance are mainly caused by the effect of sun glint and waves. The radiance or reflectance of more sun-glinted areas are increased approximately 1.5 times and the standard deviations are also increased three times compared to the less sun glinted areas. Through this study, the further studies of sun glint correction method in coastal water using the patterns of wave propagation and diffraction will be placed.

• Journal of Ginseng Research
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• 제41권4호
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• pp.602-607
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• 2017

Background: Panax ginseng is a traditional herb used for medicinal purposes in eastern Asia. P. ginseng contains various ginsenosides with pharmacological effects. In this study, floralginsenoside A (FGA), ginsenoside Rd (GRD), and ginsenoside Re (GRE) were purified from P. ginseng berry. Methods: Chemical structures of FGA, GRD, and GRE were determined based on spectroscopic methods, including fast atom bombardment mass spectroscopy, ID-nuclear magnetic resonance, and infrared spectroscopy. Inhibitory activities of these compounds on melanogenesis were studied by measuring the expression of protein and melanin content in the melan-a cell line. This inhibitory activity was confirmed by observing pigmentation and tyrosinase activities of zebrafish. Results: GRD, GRE, and FGA were not cytotoxic at concentrations less than $20{\mu}M$, $80{\mu}M$, and $160{\mu}M$ in melan-a cells, respectively. GRD, GRE, and FGA inhibited melanin biosynthesis in melan-a cells by 15.2%, 22.9%, and 23.9% at $20{\mu}M$, $80{\mu}M$, and $160{\mu}M$, respectively. FGA was observed to display the most potent inhibitory effect. In addition, FGA decreased microphthalmia-associated transcription factor protein expression in a dose-dependent manner. Moreover, FGA induced extracellular signal-regulated kinase phosphorylation level in melan-a cells. In addition, melanin pigment content and tyrosinase activity in zebrafish treated with FGA at $160{\mu}M$ were reduced. Conclusion: FGA showed the most potent inhibition of melanogenesis in both in vitro and in vivo studies. This study suggests that FGA purified from P. ginseng may be an effective melanogenesis inhibitor.