• 생명과학회지
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• 제9권4호
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• pp.453-459
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• 1999

Baculovirus(WSBV) was isolated from infected Penaeide was collected from shrimp farm at southern sea of Korea from 1993 to 1995. The Infectious virus was purified and used for diagnosis of infected shrimp. Anti-viral serum were used for immunological detection as enzyme linked immunoabsorbent assay(ELISA) and indirect fluorescent antibody technique(IFAT). In IFAT, stomach, lymphoid organ and antenae gland of infected shrimp showed fluorescent reaction. In ELISA, tissues of spontaneously infected shrimp appeared higher O.D. values than in artificial infected shrimp. Primer set was constructed from sequence of 420bp of cloned Baculovirus(WSBV) genome. Specific band for infected shrimp was detected in Polymerase chain reaction(PCR)

• 대한수의학회지
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• 제6권1호
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• pp.31-36
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• 1966

The free amino acid contents in several tissues of swine were analyzed qualitatively by means of two dimentional paper chromatography. The tissues used were liver, kidney and spleen that were obtained from normal, immunized and hog cholera infected swines. The results obtained are as follows: 1. Liver: 20 amino acids were detected in normal, 17 in immunized and 15 in infected swines. 2. Kidney: 16 amino acids were detected in normal, 13 in immunized and infected swines. 3. Spleen: 15 amino acids were detected in normal in immunized and 13 in infected swines. 4. Glutamic acid, leucine, serine and threonine were present in high concentration in all of the cases examined. 5. The free amino acids were appeard to be decreased in the infected tissues with hog cholera virus.

• 한국동물위생학회지
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• 제19권2호
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• pp.180-184
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• 1996

The present study was conducted to investigate for the hematological test and treatment effect of cattle infected with theileriosis in Cheju area. The results were summarized as fellows 1. Symptom of cattle infected with theileriosis were lose of appetite, depression, jaundise, anemia and fever. 2. A postmortem sing of cattle infected with theileriosis were not observed exept for jaundise of subcutaneous, expansion of gallbladder. 3. In the hematological test results of cattle infected with theileriosis supposed lese then killed so far as RBC number of 1, 000, 000/mm and if treated with RBC number of 2, 000, 000/mm cattle was able to recovery 4. In the treatment of cattle infected with theileriosis recovered after 90 days of treatment and the precaution against a shock of blood transfusion were able to the control of injection time and blood qantity.

• Journal of Chest Surgery
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• 제56권2호
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• pp.136-139
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• 2023

Although cardiac myxoma is one of the most common types of benign cardiac tumors, infected cardiac myxoma is very infrequent. The diagnosis of infected cardiac myxoma may be challenging because the presenting symptoms are non-specific and established management guidelines are lacking. This report describes a 39-year-old woman with a 5-month history of uncontrolled fever, chills, and myalgia who was diagnosed with myxoma and underwent mass excision. Although blood and urine cultures were negative for growing bacteria, a pathologic examination showed that the excised mass was a left atrial myxoma, with pan-bacterial polymerase chain reaction (PCR) of the surgical specimen revealing Haemophilus parainfluenzae at 99.87%, resulting in a diagnosis of infected cardiac myxoma. Laboratory tests, such as PCR, may supplement culture results in the diagnosis of infected cardiac myxoma.

• 대한수의학회지
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• 제40권3호
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• pp.575-585
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• 2000

This study was performed to observe the influence of host immune response on the chemotherapy of mice experimentally infected with Fasciola hepatica. Following immunosuppression with prednisolone or immunoenhancement with Freund's complete adjuvant(FCA), mice were experimentally infected with 3 Fasciola hepatica metacercariae and treated with closantel at 1 week post infection. In the group of mice infected with metacercariae alone, 2 mice of 10 were dead at 10 weeks post infection(20% mortality), and adult flukes were recovered from the liver and the peritoneal cavity of the remaining 8 mice(100% infectivity). In the group of mice treated with prednisolone and infected with metacercariae, 8 of 10 mice died before euthanasia with a mean time of death earlier than the control group (p<0.05). In the group of immunosuppressed mice infected with metacercariae and treated with closantel 20mg/kg, 4 of 10 mice died before sacrifice. In the group of mice infected and treated with closantel 20mg/kg, mortality and infectivity was 10% and 30%, respectively. Similar results were observed in mice infected and treated with closantel 5mg/kg which resulted in 10% and 50% mortality and infectivity, respectively. These results indicated that the efficacy of closantel treatment was decreased in immunosuppressed mice, while the pathogenicity was increased. In immunoenhanced mice infected with metacercariae, on the other hand, the efficacy of chemotherapy with both 5mg/kg or 20mg/kg closantel resulted in only 10% infectivity. The results shown in this study strongly suggest that a close interaction between chemotherapy against F hepatica with closantel and the host immune system exists. Considering that fascioliasis is a zoonosis, treatment regimen against the infection to immunosuppressed patients may require a concurrent prescription of an appro-priate immuno-enhancing adjuvant.

• 한국동물위생학회지
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• 제20권1호
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• pp.19-26
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• 1997

The present study was conducted to investigate the general epidemiological situations with 18-pullorum infected chickens from Kyongbuk provinces during the period from June 1995 to January 1996. On the Salmonella pullorum isolation tests by rectal swab culture method from infected chickens (386-samples), any Salmonella spp was not isolated from infected live-birds. But 2-S pullorum were isolated of 2-dead chickens(33.3% ) from 6-dead chickens which were positively reacted by serological tests. On the other hand, we could not isolated any Salmonella spp. in any parts of egg-contents ; egg-shell, egg-white and egg-yolks with 25-infected bird eggs. On the tests of antibiogram, 2-S pullorum strains were highly sensitive to GM, AM, SXT, CZ, K, FIM, ENR, C, AN, N, NN, LIN＋SP, CF, TE and PB, respectively and intermediate sensitive to the CB, CFP, CL, S, P and XNL. But 2-strains were resistant to CC, DP, E, L, OX, TLA and TyLO. In the serological tests, pullorum antibody titers of 18-infected birds was from 2.76 to 9.18 with average by the microplate test. During the 6-months, pullorum antibody average titers were not changed generally. To validate the effects of the antimicrobial agent treatments to the serological antibody titers, infected 6-chickens was medicated with 0.5%-futazolidone. The titer of premeditated birds was average 4.26 but after medication with furazolidone, the titers of treated 6-birds was average 4.08.

• Journal of Microbiology and Biotechnology
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• 제23권11호
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• pp.1627-1635
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• 2013

Mitochondria often play central roles in apoptotic pathways, and disruption of the mitochondrial transmembrane potential (${\Delta}{\psi}m$) has been observed in various cells undergoing apoptosis. Human cytomegalovirus (HCMV) infection induces apoptosis in permissive cells; however, investigations of mitochondria-targeted apoptosis in HCMV-infected human foreskin fibroblast (HFF) cells have been limited. Here, we investigated the mitochondrial apoptosis pathway in HCMV-infected HFF cells. Flow cytometry analysis using JC-1 revealed that HCMV infection induces disruption of ${\Delta}{\psi}m$ in HFF cells when administered 24 h post-infection (hpi), and this disruption was maximized at 48 hpi. Moreover, cytochrome c, normally a mitochondrial inner membrane protein, was detected in cytoplasmic extracts of HCMV-infected cells, but not mock-infected cells, by western blot analysis at 24 hpi. A caspase activity assay based on fluorescence spectrophotometry using a fluorogenic substrate revealed an increase in caspase-3 activity at 48 hpi in HCMV-infected cells. Caspase-8 activity was increased at 72 hpi in HCMV-infected cells. These results imply that HCMV infection induces mitochondria-mediated apoptosis in HFF cells.

• 대한수의학회지
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• 제33권2호
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• pp.301-308
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• 1993

Three or 7day old piglets were infected experimentally with different encephalomyocarditis virus isolates to detect the viral antigen by the immunoperoxidase technique and to observe strain difference in their pathogenecity in newborn pigs by comparing clinical signs and pathologic lesions. Clinical signs of the infected pigs were different depending on the virus strain, pig age and infection route. Encephalomyocarditis virus(EMCV) NVSL-PR isolate was more pathogenic than MN-25 and MN-30 isolate. Three day old piglets showed more severe illness than 7 day old piglets. Predominant clinical signs were sudden death without noticeable clinical signs and dyspnea manifested as heavy abdominal breathing. Contact-infection from infected piglets to controls was observed in the oro-nasally infected group but not the intramuscular group. Common necropsy findings of dead piglets in both age groups infected with MN-25 and NVSL-PR were accumulation of excessive fluid in the body cavities and mild to diffuse necrotic areas observed in the hearts and occasionally in the livers. Microscopically, myocarditis with inflammatory cell infiltration, necrosis of the myocardial muscle fibers and occasional mineralization were observed along with interstitial pneumonia and centrolobular necrosis in the liver. Using an immunoperoxidase technique, viral antigen was detected in myocardial muscle fibers of piglets infected with EMCV.

• Fisheries and Aquatic Sciences
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• 제15권3호
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• pp.233-239
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• 2012

Hematological analysis can provide crucial information for monitoring the health of fish. However, there is no current information available regarding hematological changes in olive flounder following infection by Vibrio scophthalmi. In this study, hematological and biochemical alterations were determined in olive flounder infected by the high virulence strain (HVS) and low virulence strain (LVS) of V. scophthalmi. Survival in serum, skin mucus, and macrophages of olive flounder was also compared between the HVS and LVS. The results demonstrated that the hematocrit value in infected fish declined from 23.4% at 0 h to 18.0% at 168 h post infection. The total protein concentration in fish infected with the HVS was significantly higher than in fish infected with the LVS and a non-infected control. Lysozyme activity was significantly different between infected and control fish. The HVS survived in serum and cell numbers increased substantially, while cell numbers of the LVS in serum decreased. These changes in hematological characteristics in fish infected by V. scophthalmi can be used as an effective and sensitive index to monitor the physiological and pathological conditions of fish. The survival and reproduction of V. scophthalmi in host serum, skin mucus, and macrophages play a major role in systemic infection and can serve as a virulence indicator for different strains.

• 한국산림과학회지
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• 제102권4호
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• pp.499-505
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• 2013

소나무재선충병(Bursaphelenchus xylophilus)은 한국, 일본, 그리고 중국과 같은 동아시아의 소나무류 산림에 심각한 피해를 유발시킨다. 재선충병에 의한 병해는 그 치료방법이 훈증, 소각외에는 거의 없기 때문에 감염목을 조기 탐지하여 그 피해가 주변 임목 및 임분에 확산되지 않도록 하는 것이 최선책이라 할 수 있다. 본 연구에서는 소나무재선충병 감염목과 비감염목의 분광반사율을 주기적으로 측정하여 감염목의 분광반사 특성을 구명하였다. 이를 위하여, 6월에서 10월까지 GER3700 spectroradiometer를 이용하여 감염목과 비감염목의 400 nm~2,500 nm 파장대의 분광반사값을 측정하였다. 측정된 값의 noise를 보정하기 위하여 cubic spline 보간법을 사용하였다. 그 결과, 대부분의 감염목들은 재선충 주입 후 2개월 이내에 적색(600 nm~700 nm) 그리고 중적외선(1,400 nm~1,500 nm) 파장대에서 분광반사값의 변화를 보였으나, 비감염목들의 분광반사값은 어느 파장대에서도 특별한 변화가 나타나지 않았다. 시기별로 감염목과 비감염목의 분광반사값 변화를 통계적으로 비교해 본 결과, 소나무재선충 주입 2달 후 적색 파장대역과 중적외선 파장대역에서 가장 빠르게 통계적으로 유의한 차이를 보였으나(p<0.05), 근적외선 파장대역에서는 통계적으로 유의한 차이가 나타나지 않았다(p>0.05). 따라서 적색과 중적외선 파장대역에서의 분광반사율 변화를 통해 소나무재선충병 감염목의 조기 탐지가 가능한 것으로 판단되었다.