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Distribution of Protease Inhibitors from Fish Eggs as Seafood Processing Byproducts (어류 알의 Protease Inhibitor 활성 분포)

  • Ji, Seong-Jun;Lee, Ji-Sun;Shin, Joon-Ho;Park, Kwon-Hyun;Kim, Jin-Soo;Kim, Kyoung-Sub;Heu, Min-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.1
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    • pp.8-17
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    • 2011
  • To identify and examine the distribution of proteolytic inhibitory activity in crude extracts from fish eggs, and to determine the applicability of these protease inhibitors as anti-degradation agents in surimi-based products and fish meat, we compared the inhibitory activities of various extracts from fish eggs to those of commercial proteases, such as trypsin and papain. We used the optimal conditions for the screening of trypsin activity: 30 ug/uL of 0.1% trypsin and 0.6 mM Na-benzoyl-L-arginine-p-nitroanilide (BAPNA) with a pH of 8.0 at $40^{\circ}C$ for 60 min. The activities of papain and four commercial proteases were investigated after mixing with 100 ug/uL enzymes and 0.3% casein with a pH of 8.0 at $40^{\circ}C$ for 60 min. We performed a screening assay to detect the inhibitory activity (%) of crude extracts from eight species of fish eggs against the target proteases trypsin and papain. The assay revealed a wide distribution of trypsin and papain inhibitors in fish eggs. The specific inhibitory activities (11.6.28.6 U/mg) of crude extracts from fish eggs against trypsin and BAPNA substrate were higher than that (0.64 U/mg) of egg whites, used as a commercial inhibitor. The inhibitory activities of crude extracts from fish eggs against trypsin, and of egg whites against casein substrate (1.94.4.51 U/mg), were higher than those of papain (0.24.1.57 U/mg) and commercial protease (0.04.0.32 U/mg). The extracts from fish eggs were rich in protease inhibitors that exhibited strong inhibitory activity against trypsin, a serine protease, and papain, a cysteine protease.

Quality of Surimi from Unmarketable Bastard Halibut as Affected by the Region where Cultured (양식지역의 차이에 따른 비규격 넙치 연육 (Surimi)의 품질 특성)

  • Heu, Min-Soo;Shin, Jun-Ho;Park, Kwon-Hyun;Lee, Ji-Sun;Noe, Yu-Ni;Jeon, You-Jin;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.6
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    • pp.598-605
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    • 2010
  • This study investigated the physicochemical and enzymatic properties of unmarketable bastard halibut (Paralichthys olivaceus) cultured in different regions (i.e., Jeju, Wando, and Geoje) as a potential source of surimi and surimi gel. The proximate composition of unmarketable bastard halibut cultured in different regions did not differ significantly at P<0.05. Compared to Alaska pollock muscle, all of the unmarketable bastard halibut muscle had a 4% higher crude protein content and 5% lower moisture content. The collagen content of bastard halibut muscle cultured in Jeju was 1.96 g/100 g, which was higher than in fish cultured in other regions. Regardless of the region where cultured or pH, the enzymatic activities of the crude extracts from unmarketable bastard halibut muscle ranged from 0.30.0.48 U/mg for casein and hemoglobin, 11.9.13.7 U/mg for LeuPNA, 5.6.6.7 U/mg for ArgPNA, 2.8.4.7 U/mg for SAAPFNA, and 0.1.0.2 U/mg for BAPNA. Regardless of region, no mercury or lead was found in any of the unmarketable bastard halibut muscle, except for lead in fish cultured in Geoje. The strength of surimi gels from unmarketable bastard halibut cultured in Jeju, Geoje, and Wando was 1059, 988, and 900 g${\times}$cm, respectively. The surimi gel from unmarketable bastard halibut cultured in Jeju was stronger than commercial Alaska pollock surimi, which was grade SA.

A Study on Worker Exposure Level and Variation to Asbestos in some Asbestos Industries (일부 석면취급사업장의 석면폭로 농도 및 작업환경관리 기준에 관한 연구)

  • Oh, Se Min;Shin, Yong Chul;Park, Doo Yong;Park, Dong Uk;Chung, Kyou Chull
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.3 no.1
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    • pp.100-109
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    • 1993
  • This study was performed to evaluate the asbestos exposure levels and variations in textile, brake lining manufacturing and slate manufacturing industries. For this study, fifteen plants of brake lining manufacturing industry, 7 plants of textile industry, and 2 plants of slate manufacturing industry were selected and surveyed. Geometric means (GMs) of airborne asbestos concentrations in textile, brake lining manufacturing, and slate manufacturing industries were 1.42 f/cc(0.07-6.1O f/cc), 0.19 f/cc(<0.01-2.67 f/cc) and 0.08 f/cc(0.02-0.67 f/cc), respectively. In textile industry overall GMs of airborne asbestos concentrations in plants with less than 50 workers and in plants with more than 50 workers were 1.60 f/cc and 0.3 f/cc, respectively. Therefore, the size of plant showed some difference in the airborne asbestos concentrations. Three out of 7(42.9%) exceed the Korean standard, 2 f/cc, and every plant exceed the USA standard, 0.2 f/cc of the OSHA-Permissible Exposure Level(OSHA-PEL). Especially, one plant showed the highest average concentration of 2.87 f/cc. In brake lining manufacturing industry, the plants with less than 50 workers showed 0.22 f/cc. The plants with more than 50 workers showed 0.18 f/cc. All plants showed the exposure level below the Korean standard. Five of 15 (33.3%) were above the OSHA-PEL. One plant showed the highest average concentraton of 0.84 f/cc. In slate manufacturing industry, the average exposure level was 0.08 f/cc, and all of the plants were below the Korean standard and the OSHA-PEL.

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Analysis of Machinery Part Industry Trends and its supporting scheme - Focused on Chung-buk Area (동력기반 기계부품 산업 현황 분석 및 지원방안 - 충북지역을 중심으로)

  • Lee, Hyoungwook;Bae, SungMin
    • Journal of Institute of Convergence Technology
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    • v.6 no.1
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    • pp.27-30
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    • 2016
  • In recent years, manufacturing industry takes a lot of significant change - such as Industry4.0, SMART factory, and IoT(Internet of things). To satisfy their requirements, many cutting-edge machineries are provided to enhance manufacturing industry's productivity. Also, IT technologies are applied to many machinery part and improve their functions. In Korea, Chung-buk plays an important role in machinery part industry. In this paper, we survey, analyze and summarize the current machinery part industry focused on Chunk-buk Area.

Effect of Adding Graphene/Carbon Nanotubes (FCN) on the Mechanical Properties of Polyamide-Nylon 6 (그래핀/탄소나노튜브(FCN) 첨가에 따른 Polyamide-Nylon 6의 기계적 특성에 미치는 영향)

  • Seung-Jun Yeo;Hae-Reum Shin;Woo-Seung Noh;Man-Tae Kim
    • Journal of the Korean Society of Industry Convergence
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    • v.26 no.6_3
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    • pp.1297-1303
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    • 2023
  • Research on enhancing the mechanical strength, lightweight properties, electrical conductivity, and thermal conductivity of composite materials by incorporating nano-materials is actively underway. Thermoplastic resins can change their form under heat, making them highly processable and recyclable. In this study, Polyamide-Nylon 6 (PA6), a thermoplastic resin, was utilized, and as reinforcing agents, fused carbon nano-materials (FCN) formed by structurally combining Carbon Nanotube(CNT) and Graphene were employed. Nano-materials often face challenges related to cohesion and dispersion. To address this issue, Silane functional groups were introduced to enhance the dispersion of FCN in PA6. The manufacturing conditions for the composite materials involved determining the use of a dispersant and varying FCN content at 0.05 wt%, 0.1 wt%, and 0.2 wt%. Tensile strength measurements were conducted, and FE-SEM analysis was performed on fracture surfaces. As a result of the tensile strength test, it was confirmed that compared to pure PA6, the strength of the polymer composite with a content of 0.05 wt% was improved by about 60%, for 0.1 wt%, about 65%, and for 0.2 wt%, the strength was improved by 50%. Also, when compared according to the content of FCN, the best strength value was shown when 0.1 wt% was added. The elastic modulus also showed an improvement of about 15% in the case of surface treatment compared to the case without surface treatment, and an improvement of about 70% compared to pure PA6. Through FE-SEM, it was confirmed that the matrix material and silane-modified nanomaterial improved the dispersibility and bonding strength of the interface, helping to support the load evenly and enabling effective stress transfer.

Characteristics Analysis of Burned tree by Terrestrial LiDAR in Forest Fired Area of Pinus densiflora (지상라이다를 활용한 소나무 산불피해지의 임목 피해특성 분석)

  • Kang, Jin-Taek;Ko, Chi-Ung;Yim, Jong-Su;Lee, Sun-Jeoung;Moon, Ga-Hyun;Lee, Seung-Hyun
    • Korean Journal of Remote Sensing
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    • v.36 no.6_1
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    • pp.1291-1302
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    • 2020
  • To verify the field-effectiveness of Terrestrial Laser Scanner (TLS), a terrestrial LiDAR was deployed to examine the damage properties of woods in forest fire area, then the data was compared with the results surveyed by a forestry expert. Four sample plots (30 m × 50 m, 0.15 ha) were set from the foot to the top of the mountain, and DBH, height, clear length, burned height, and crown length were investigated. Next, TLS collected information on damage characteristics found in the sample plots. This information was then compared with that amassed by the expert. The expert and the TLS survey results showed 30.8 cm and 29.9 cm for DBH, 15.8 m and 17.5 m for tree height, 8.4 m and 8.4 m for clear length, 4.0 m, 3.5 m for burned height, and 7.4 cm and 9.1 cm for crown length. With the exceptions of height and clear length, no notable discrepancy was observed between two methods. H/D ratio, CL/H ratio, and BH/CL ratio, all of which contribute to stability and decay rate of the stand, from the two methods were also compared. The human survey rated each ratio (H/D, CL/H, BH/CL in order) 51.3%, 47.1%, and 53.6%, while the TLS presented the results of 58.8%, 52.0%, and 38.7%.

Antibacterial Effect of PVP/PEG/Carrageenan/Silver Acetate Hydrogels by γ-ray (γ-ray를 이용하여 합성한 PVP/PEG/Carrageenan/Silver acetate 하이드로겔의 항균효과)

  • Lim, Youn-Mook;Youn, Young;Gwon, Hui-Jeong;Park, Jong-Seok;Nho, Young-Chang
    • Journal of Radiation Industry
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    • v.4 no.1
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    • pp.73-77
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    • 2010
  • In recent day, there is much interest in the biocidal activity of silver since silver is known to be safe and effective as disinifectant and biocidal material against coliforms and viruses. In this study, hydrogels containing silver acetate as antibacterial agent have been prepared using gamma rays irradiation. The hydrogels are composed of poly(vinyl pyrrolidone) (PVP), poly(ethylene glycol) (PEG), carrageenan and silver acetate. The concentration of solution was 9 wt%. The ratio of PVP : PEG : carrageenan was 6 : 1 : 2. The concentration of the silver acetate were 0, 0.01, 0.03, 0.05 and 0.07% and Gamma irradiation dose was 25 kGy. The Gamma irradiation dose in hydrogels with 0.01% silver acetate were 20 kGy, 35 kGy, 50 kGy, 65 kGy, and 80 kGy. The results showed that 0.01% silver acetate concentration of hydrogels by 25 kGy irradiation dose showed the highest antibacterial activity against E. coli and Staphylococcus aureus. Moreover, antibacterial activity of various Gamma irradiation dose in hydrogels treated 0.01% silver acetate showed highest 35 kGy irradiation dose against Staphylococcus aureus.

Net energy content of rice bran, corn germ meal, corn gluten feed, peanut meal, and sunflower meal in growing pigs

  • Li, Yakui;Li, Zhongchao;Liu, Hu;Noblet, Jean;Liu, Ling;Li, Defa;Wang, Fenglai;Lai, Changhua
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.9
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    • pp.1481-1490
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    • 2018
  • Objective: The objective of this experiment was to determine the net energy (NE) content of full-fat rice bran (FFRB), corn germ meal (CGM), corn gluten feed (CGF), solvent-extracted peanut meal (PNM), and dehulled sunflower meal (SFM) fed to growing pigs using indirect calorimetry or published prediction equations. Methods: Twelve growing barrows with an average initial body weight (BW) of $32.4{\pm}3.3kg$ were allotted to a replicated $3{\times}6$ Youden square design with 3 successive periods and 6 diets. During each period, pigs were individually housed in metabolism crates for 16 d, which included 7 days for adaptation. On d 8, the pigs were transferred to the respiration chambers and fed one of the 6 diets at 2.0 MJ metabolizable energy (ME)/$kg\;BW^{0.6}/d$. Total feces and urine were collected and daily heat production was measured from d 9 to d 13. On d 14 and d15, pigs were fed at their maintenance energy requirement level. On the last day pigs were fasted and fasting heat production was measured. Results: The NE of FFRB, CGM, CGF, PNM, and SFM measured by indirect calorimetry method was 12.33, 8.75, 7.51, 10.79, and 6.49 MJ/kg dry matter (DM), respectively. The NE/ME ratios ranged from 67.2% (SFM) to 78.5% (CGF). The NE values for the 5 ingredients calculated according to the prediction equations were 12.22, 8.55, 6.79, 10.51, and 6.17 MJ/kg DM, respectively. Conclusion: The NE values were the highest for FFRB and PNM and the lowest in the corn co-products and SFM. The average NE of the 5 ingredients measured by indirect calorimetry method in the current study was greater than values predicted from NE prediction equations (0.32 MJ/kg DM).

Effects of Plane of Nutrition on Growth Performance and Meat Quality Traits in Finishing Pigs

  • Ha, Duck-Min;Park, Byung-Chul;Park, Man Jong;Song, Young Min;Jin, Sang Keun;Park, Jae Hong;Lee, C. Young
    • Journal of Animal Science and Technology
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    • v.54 no.6
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    • pp.449-454
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    • 2012
  • The present study was performed to investigate the effects of the plane of nutrition on growth performance and meat quality traits, especially marbling and PSE (pale, soft, and exudative), in finishing pigs. One hundred and four barrows and 102 gilts born to Yorkshire ${\times}$ Landrace dams and Duroc sires were randomly allocated to 16 pens at approximately 80-kg body weight, with 13 animals housed per pen except for two pens. Each eight pens received a diet containing either 3.4 Mcal ME/kg and 0.98% total lysine (high plane) or 3.1 Mcal ME/kg and 0.81% total lysine (medium plane). All animals were slaughtered at approximately 112 kg BW on an average, after which the Longissimus muscle (LM) was analyzed physicochemically. The ADG, ADFI, gain:feed as well as marbling score did not differ between the two dietary groups, whereas backfat thickness was greater in the high-plane group than in the medium-plane group (24.1 vs. 22.5 mm P<0.05). No LM exhibited PSE, i.e. $L^*$ (lightness) > 50, pH < 6.0 and drip loss > 5.0%, nor were these PSE-related variables influenced by the dietary treatment. Contents of crude protein and fat in LM did not differ between the two dietary groups. In conclusion, the present results suggest that growth performance and the occurrence of the PSE or 'watery' carcass are not influenced significantly by increasing the plane of nutrition in finishing pigs when the finisher is adequate nutritionally for supporting maximal lean growth. However, increasing the plane of nutrition is likely to result in an increase in fat deposition.

Basic Study on the Regenerator of Stirling Engine (IV) - Heat Transfer and Flow Friction Characteristic of the Regenerator with Steel Wire Matrix - (스털링 기관용 재생기에 관한 기초 연구 (IV) - 철선을 축열재로 한 재생기의 전열 및 유동손실 특성 -)

  • Oh D. G.;Kim T. H.
    • Journal of Biosystems Engineering
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    • v.30 no.4 s.111
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    • pp.202-209
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    • 2005
  • The output of Stirling engine is influenced by the regenerator effectiveness. The regenerator effectiveness is influenced by heat transfer and flow friction loss of the regenerator matrix. In this paper, in order to provide a basic data for the design of regenerator matrix, characteristics of heat transfer and flow friction loss were investigated by a packed method of matrix in the oscillating flow as the same condition of operation in a Stirling engine. As matrices, 6 kinds of steel wires, 4 kinds of combined steel wires, 8 kinds of combined steel wires with screen meshes were used. The results are summarized as follows; Among 6 kinds of steel wires $({\phi}0.7\;mm,\;{\phi}0.9\;mm,\;{\phi}1.2\;mm,\;{\phi}\;1.6\;mm,\;{\phi}2.0\;mm,\;{\phi}2.7\;mm),$ the two steel wires $({\phi}0.7\;mm,\;{\phi}0.9\;mm)$ showed the highest in effectiveness. Among 4 kinds of combined steel wires $({\phi}l.6-{\phi}l.2\;mm,\;{\phi}1.2-{\phi}l.6\;mm,\;{\phi}0.9-{\phi}l.2\;mm,\;{\phi}l.2-{\phi}0.9\;mm),\;the\;{\phi}1.2-{\phi}0.9\;mm$ showed the highest in effectiveness. Among 8 kinds of combined steel wires with screen meshes $(150-{\phi}0.9\;mm,\;150-{\phi}l.2\;mm,\;{\phi}0.9\;mm-150,\;{\phi}1.2\;mm-150,\;150-{\phi}0.9\;mm-150,\;150-{\phi}1.2\;mm-150,\;150-{\phi}l.6\;mm-150,\;150-{\phi}2.0\;mm-150),\;the\;{\phi}l.2\;mm-150$ showed the highest in effectiveness.