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미더덕 추출물을 첨가한 조피볼락용 사료의 제조 (Preparation of Rockfish (Sebastes schlegelii) Feed Fortification with Mideodeok (Styela clava) Extracts)

  • ;윤보영;강석중;최영준;최병대
    • 한국수산과학회지
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    • 제41권6호
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    • pp.458-465
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    • 2008
  • The nutritional contribution of mideodeok extracts (ME) on rockfish (Sebastes schlegelii) feed and fish muscle was investigated. Different concentrations of the ME mixed with commercial diet were fed to mature rockfish for 8 weeks. The lipid and ash contents of the formulated diets were relatively similar to the control diet, while increasing the extract concentration increased the moisture content and decreased the protein contents. Major fatty acid components (C18:1n-9, 16:0, C20:5n-3, C22:6n-3) were of comparable quantity. High presence of C18:2n-6 was attributed to soybean oil incorporated in the diets, while the essential fatty acids were within limits (0.9-1.0%). The diet fortified with 6% ME produced the highest feed efficiency, with increased protein content in the muscle as well as lipid content for both muscle and liver. Hepato- and visceral-somatic index values were elevated with increasing ME concentration Muscle fatty acid contents were mostly C18:1n-9 and C16:0, with low absorption of C18:2n-6 in both the muscle and liver. Total highly unsaturated fatty acid content was significantly reduced in the fish muscle, but the values were higher for fish fed with a ME-fortified diet. An increasing trend for eicosapentaenoic acid and docosahexaenoic acid was also observed with increased ME fortification, with liver levels of these compounds remaining within range throughout the duration of the experiment.

시판 조미 멍게(Halocynthia roretzi)의 식품학적 품질 특성 (Food Quality and Characterization of Commercial Seasoned Sea Squirt Halocynthia roretzi)

  • 이정석;김민지;이지선;김지혜;김기현;김현정;허민수;김진수
    • 한국수산과학회지
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    • 제46권1호
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    • pp.10-17
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    • 2013
  • This study investigated the food biochemical characterization of commercial seasoned sea squirt Halocynthia roretzi (CSS). The proximate composition of CSS was 77.2-82.7% moisture, 7.1-9.1% crude protein, 0.3-2.6% crude lipid and 3.5-6.3% ash. Taste compound contents of CSS were 2.3-5.4% salinity (saltiness), 0.42-1.12 g/100 g total acidity (sourness) and 114.9-330.2 mg/100 g amino nitrogen (taste intensity). The Hunter color values of CSS were 23.79-32.50 for lightness, 9.97-20.45 for redness, 14.01-20.96 for yellowness and 64.50-76.63 for color difference. The odor intensity of CSS was 35.0-62.0. According to these results, there were large differences in proximate composition, taste compounds, Hunter color values and odor intensity of CSS. Viable cell counts ranged from 6.20 to 7.69 log (CFU/g), and most of the viable cells comprised of lactic acid-forming bacteria. CSS was not detected in the coliform group.

Effect of L-Carnitine and Source of Dietary Fat on Growth Performance and Serum Biochemical Parameters of Piglets Weaned at 35 Days of Age

  • Li, Defa;Qiao, Q.;Johnson, E.W.;Jiang, J.;Wang, F.;Blum, R.;Allee, G.
    • Asian-Australasian Journal of Animal Sciences
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    • 제12권8호
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    • pp.1263-1272
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    • 1999
  • The effects of carnitine in diets with or without added fat (5% lard or soybean oil) were evaluated in 72 Large White ${\times}$ Landrace ${\times}$ Duroc pigs weaned at 35 days of age. Pigs were fed a 1.30% lysine corn-soybean basal diet+15% dried whey+4% fish meal with carnitine at 0 or 50 mg/kg and either 0% added fat, 5% soybean oil or 5% lard for 6 weeks in a $2{\times}3$ factorial trial (6 treatments, 3 pens per treatment, 4 pigs per pen). Addition of carnitine increased average daily gain (ADG) and average daily feed intake (ADFI) in the second two weeks of the six-week trial and overall, but had no significant effect on feed per gain (F/G). Lard alone depressed ADG (p<0.05) in the last two weeks of the trial and overall, but the ADG for pigs fed lard+carnitine was similar to the control. Lard reduced feed intake in the first two weeks of the trial (p<0.05). Carnitine reduced the percentage of pigs with poor (ADG<375 g/d) growth (15 vs 40%; p<0.05). The greater uniformity of growth was most evident in low-weaning-weight pigs in the second period (16 vs 62%, p<0.005). Addition of fat did not produce any positive effect on uniformity and had no interaction with carnitine on uniformity. Carnitine addition increased serum total carnitione and short-chain acyl-carnitine levels (p<0.05), but did not modify free carnitine levels. Serum carnitine levels were lower at weaning than at 14, 28, or 39 days after weaning (p<0.05). Carnitine increased serum protein levels on day 14 (p<0.05). Addition of fat in the form of soybean oil or lard did not improve piglet growth performance. Addition of 50 mg/kg of carnitine to the diet of weanling pigs enhanced postweaning performance.

Oligosaccharides are a key factor in prediction of amino acid digestibility in soybean meal of different origins when fed to growing pigs

  • Li, Zhongchao;Wang, Qiuyun;Xie, Fei;Liu, Dewen;Li, Yakui;Lyu, Zhiqian;Lai, Changhua
    • Asian-Australasian Journal of Animal Sciences
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    • 제30권12호
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    • pp.1724-1732
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    • 2017
  • Objective: The objective of this experiment was to determine apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of crude protein (CP) and amino acid (AA) in 15 sources of soybean meal (SBM) produced from soybeans from different countries and subsequently to establish equations for predicting the AID and SID in SBM based on their chemical composition. Methods: Eighteen barrows ($57.9{\pm}6.1kg$) fitted with a simple T-cannula were allotted into three $6{\times}6$ Latin square designs. Each period comprised a 6-d adaption period followed by a 2-d collection of ileal digesta. The 15 test diets included SBM as a sole source of AA in the diet. Another nitrogen-free diet was used to measure basal endogenous losses of CP and AA. Chromic oxide (0.3%) was used as an inert marker in each diet. Results: The AID of lysine in SBM from China and USA tended to be greater than in SBM from Brazil (p<0.10). The SID of valine and proline in SBM from China was greater than in SBM from Brazil (p<0.05). The SID of lysine, threonine, cysteine and glycine in SBM from China tended to be greater than in SBM from Brazil (p<0.10). From a stepwise regression analysis, a series of AID and SID prediction equations were generated. The best fit equations for lysine in SBM were: AID lysine = 1.16 sucrose-1.81 raffinose+82.10 ($R^2=0.69$, p<0.01) and SID lysine = 1.14 sucrose-1.93 raffinose-0.99 ether extract (EE)+85.26 ($R^2=0.77$, p<0.01). Conclusion: It was concluded that under the conditions of this experiment, the oligosaccharides (such as sucrose and raffinose) can be used to predict the AID and SID of AA in SBM with reasonable accuracy.

Effects of Adding Super Dose Phytase to the Phosphorus-deficient Diets of Young Pigs on Growth Performance, Bone Quality, Minerals and Amino Acids Digestibilities

  • Zeng, Z.K.;Wang, D.;Piao, X.S.;Li, P.F.;Zhang, H.Y.;Shi, C.X.;Yu, S.K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권2호
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    • pp.237-246
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    • 2014
  • Two experiments were conducted to evaluate the efficacy of feeding an Escherichia coli (E. coli) derived phytase to pigs fed P deficient, corn-soybean meal diets. In Exp. 1, one hundred and twenty crossbred piglets ($9.53{\pm}0.84$ kg) were allocated to one of five treatments which consisted of four low P diets (0.61% Ca, 0.46% total P and 0.24% non-phytate P) supplemented with 0, 500, 1,000, or 20,000 FTU/kg E. coli phytase as well as a positive control formulated to be adequate in all nutrients (0.77% Ca, 0.62% total P and 0.42% non-phytate P). The treatments were applied to six pens with four pigs per pen for 28 days. In Exp. 2, ten crossbred pigs ($19.66{\pm}1.16$ kg) fitted with ileal T-cannula were used in a nutrient balance study. The pigs were assigned to treatments similar to those used in Exp. 1 in a doubly replicated $5{\pm}4$ incomplete Latin square design (5 diets with 4 periods). Each period consisted of a 5-d adjustment period followed by a 3-d total collection of feces and urine and then a 2-d collection of ileal digesta. Supplementation with phytase linearly increased (p<0.05) weight gain, feed intake, feed efficiency, bone breaking strength and fat-free dry and ash bone weight. There were linear increases (p<0.01) in the apparent ileal digestibility (AID) of DM, GE, CP, Ca, total P, inositol hexaphosphate ($IP_6$) and some AA with increasing dose of E. coli phytase. Pigs fed 20,000 FTU/kg had a greater (p<0.05) AID of IP6 (80% vs 59% or 64%, respectively) than pigs fed diets with 500 or 1,000 FTU/kg phytase. There were linear increases (p<0.05) in the total tract digestibility of Ca, total P, Na, K, Mg, and Zn as well as in the retention of Mg and Zn with increased phytase dose. The retention and utilization of Cu, and the total tract digestibility of CP and Cu quadratic increased (p<0.05) with increased phytase dose. In conclusion, supplementation of 500 FTU of phytase/kg and above effectively hydrolyzed phytate in low-P corn-soybean diets for pigs. In addition, a super dose of phytase (20,000 FTU/kg) hydrolyzed most of the IP6 and consequently further improved mineral use, protein utilization and performance.

Fatty Acid Compositions of Cultured Oyster (Crassostrea gigas) from Korean and Japanese Spats

  • Jeong Bo-Young;Moon Soo-Kyung;Jeong Woo-Geon
    • Fisheries and Aquatic Sciences
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    • 제2권2호
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    • pp.113-121
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    • 1999
  • Changes in lipid content, lipid class and fatty acid compositions of the cultured oysters in shallow-water, Bukman bay (Tongyeong, Korea), using both Korean and Japanese spats were investigated. The content of non-polar lipid (NL) comprised approximately $60- 80\%$ of total lipid (TL) in the cultured oysters. There was a positive correlation between NL content and meat weight, y=0.287lx-15.309 (r=0.834l, p<0.001). The prominent fatty acids of the oysters were 16:0, 20:5n-3 (eicosapentaenoic acid, EPA), 22:6n-3 (docosahexaenoic acid, DHA), 18:0, l8:1n-7, l8:1n-9, l6:1n-7, 14:0 and l6:4n-3. During the growth of the oysters, l6:4n-3 showed the highest coefficient of variation, accounting for $41.8\%$ for the Korean oyster and $32.3\%$ for the Japanese one, respectively. Both oysters showed low level of n-3 fatty acids such as DHA and EPA and high level of n-6 fatty acid, 20:4n-6, in the spawning period (August). During growth of the oysters, both EPA and DHA were the richest fatty acids in the harvest period (December, 314 mg/100g sample) and in the pre-spawning period (July, 237-247 mg/100g sample), respectively. Consequently, the cultured oyster with Japanese spat contained approximately two times more n-3 fatty acids per oyster individual than those with Korean one in the harvest season.

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어류 알로부터 Protease Inhibitors의 크로마토그래피법에 의한 분획 (Chromatographic Fractionation of Protease Inhibitors from Fish Eggs)

  • 김진수;김기현;김현정;김민지;박성환;이현지;허민수
    • 한국수산과학회지
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    • 제46권4호
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    • pp.351-358
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    • 2013
  • A protease inhibitor from fish eggs was fractionated using chromatographic methods. The fractionation efficiency was evaluated in terms of specific inhibitory activity (SIA, U/mg), purity (fold), total inhibitory activity (TIA, U), and recovery (%). The protease inhibitor (PI) from egg extracts of skipjack tuna (ST Katsuwonus pelamis), yellowfin tuna (YT Thunnus albacares) and Alaska pollock (AP Theragra chalcogramma) was fractionated using Sephadex G-50 gel filtration and DEAE-Sepharose CL-6B anion exchange chromatography based on protein size exclusion and net charge, respectively. Fractions exhibiting strong inhibitory activity were contained in the 30-50 kDa fraction on gel filtration and in the range of 0.4-0.7 M NaCl gradient fraction on anion exchange chromatography. The respective TIA and percent recovery of the fraction obtained with gel filtration toward trypsin and $N{\alpha}$-benzoyl-L-arginine-p-nitroanilide (BAPNA) were 2,758.7 U and 29.6% for ST, 1,005.5 U and 25.6% for YT, and 1,267.5 U and 26.0% for AP. Gel filtration chromatography was more effective at fractionating PI than using ion exchange chromatography. These results suggest that fish eggs act as serine protease inhibitors and might be useful for protease inhibition in foodstuffs.

미생물막 유동층 반응기를 이용한 산업폐수 처리에 관하여 (The Treatment of Industrial Wastewater by the Fluidized-Bed Biofilm Reactor)

  • 서명교;서정호;강준수
    • 한국식품과학회지
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    • 제25권1호
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    • pp.69-77
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    • 1993
  • 미생물막 유동층 반응기로 염료공장 폐수의 유기물 처리효율을 고찰하였다. 공장폐수를 2배, 3배 및 6배 희석하여 처리하였던 바, 각 희석배율 모두 F/M비가 0.2에서 0.3으로 증가할 때 처리효율이 급격히 낮아졌다. 한편, 같은 F/M비에서는 희석율이 높을수록 처리효율이 높았으며 수리학적 체류시간이 증가함에 따라서 처리효율도 증가하였다. 6배 희석시 F/M비가 0.2 이하에서 BOD 처리효율이 $90{\sim}97%$로 가장 좋았다. 또 반응기 설계를 위한 통역학적 계수 Y, $k_b$값 및 상관계수 r은 2배 희석시 $Y=0.3365\;k_d=0.03782\;day^{-1}\;r=0.997$, 3배 희석시 $Y=0.3341\;k_d=0.0275\;day^{-1}\;r=0.996$ 및 6배 희석시는 $Y=0.5460\;k_d=0.03434\;day^{-1}\;r=0.998$로 나타났다.

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전북지역(全北地域) 산업근로자(産業勤勞者)의 산업재해(産業災害)에 관(關)한 조사연구(調査硏究) (A Study on Industrial Accidents of Workers in Jeonbug Areas)

  • 황인담;박영수;서석관
    • Journal of Preventive Medicine and Public Health
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    • 제14권1호
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    • pp.89-96
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    • 1981
  • Of 2,740 industries in Jeonbug area which are covered by industrial insurance policy, 462 facilities which the accidents related to industry occured during the year of 1979 were studied. and the results are summarized as follows: 1. The accidents related to industry occured in 462 industries of the total 2,740 industrial facilities in Jeonbug area as of 1979. 2. The incidence rate of accident per 1,000 workers was 34.3 (49.2 in male workers and 12.8 in female workers), the frequency rate of the total industries in jeonbug area was 13.36, and severity rate was 1.3. 3. The frequency rates and severity rates by type of industry in study area were quite different to compare with those of national rates. 4. The incidence rate of construction industry was 223.6 per 1,000 workers, and that of transportation-communication industries were 78.6. 5. The proportion of industrial accidents of $20{\sim}24$ age group was 22.1 per cent of the total accidents, and the proportions decreased according to age increase. 6. The incidence rate in the industry having less than 49 workers was 20.6 per 1,000 workers, that of industry with $50{\sim]99$ workers was 26.7, that of industry with $100{\sim}199$ was 51.9, that of industry with $200{\sim}499$ was 80.2 and that of with more than 500 worker was 40.7. 7. The accidents which occured in the workers with experience of less than one year was 69.4 per cent of the total accidents, otherwise, the longer the workers have worked the less accident they have. 8. The most accidents occured in tile shift between 10 to 12 o'clock, and 16 to 18 hour 9. The primary causes of the industrial accidents were found to be collisions, machinery falling objects and falls. 10. The site of injury by type of industry were quite different, and the major site of injury was finger. 11. The laceration and open injuries of the accidents related to industry were 37.2 per cent of the total cases, and fractures or dislocations were 23.5 per cent, and contusions were 6.5 per cent. 12. Death rate of industrial accident was 5.0 per 10,000 workers, and those of industry were 47.6 in transportation, 42.8 in construction industry, 24.4 in mine industry, and 2.0 in manufacturing industry.

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Durian 종자 전분의 이화학적 특성 (Physicochemical Properties of the Durian Seed Starch)

  • 이성갑;김형수;손종연
    • 한국식품과학회지
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    • 제31권6호
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    • pp.1410-1414
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    • 1999
  • Durian 종자 전분의 입자크기범위는 약 $2.0-10.0\;{\mu}m$ 이었으며 구형과 다각형의 형태를 갖고 있었다. Durian종자, 옥수수, 고구마, 감자전분의 아밀로오스 함량은 28.3%, 27.5%, 20.3% 및 21.7%이었다. Durian 종자, 옥수수, 고구마, 감자전분의 blue value는 0.370, 0.368, 0.332 및 0.338이었으며, 알칼리도는 7.39, 9.02, 7.08 및 5.43이었다. Durian 종자 전분의 팽윤력과 용해도는 감자전분보다 낮았다. Durian 종자자전분의 X-선 회절도에 의한 결정성은 곡류전분과 같은 A형이었다. RVA로 측정한 durian 종자 전분의 호화개시온도 $(76.6^{circ}C)$는 옥수수$(73.0^{circ}C)$, 고구마$(72.3^{circ}C)$, 감자전분 $(70.2^{circ}C)$보다 높았으며 breakdown은 낮은 반면 setback은 높았다.

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