• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.963-977
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• 2015

The well-characterized gram-positive bacterium Bacillus subtilis is an outstanding industrial candidate for protein expression owing to its single membrane and high capacity of secretion, simplifying the downstream processing of secretory proteins. During the last few years, there has been continuous progress in the illustration of secretion mechanisms and application of this robust host in various fields of life science, such as enzyme production, feed additives, and food and pharmaceutical industries. Here, we review the developments of Bacillus subtilis as a highly promising expression system illuminating strong chemical- and temperatureinducible and other types of promoters, strategies for ribosome-binding-site utilization, and the novel approach of signal peptide selection. Furthermore, we outline the main steps of the Sec pathway and the relevant elements as well as their interactions. In addition, we introduce the latest discoveries of Tat-related complex structures and functions and the countless applications of this full-folded protein secretion pathway. This review also lists some of the current understandings of ATP-binding cassette transporters. According to the extensive knowledge on the genetic modification strategies and molecular biology of Bacillus subtilis, we propose some suggestions and strategies for improving the yield of intended productions. We expect this to promote striking future developments in the optimization and application of this bacterium.

• Journal of the Korean Society of Safety
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• v.24 no.2
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• pp.89-93
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• 2009

The muscular-skeletal disorders that have become a major issue recently in Korean industrial safety area are mainly caused by manual material handling task. The objective of this study is to provide scientific data for the establishment of work safety standard for Korean workers through the experiments of lifting task under various conditions, in order to prevent the muscular-skeletal disorders in the industrial work site. This study used the psychophysical approach to determine the maximum acceptable weight(MAWL) for seven young male subjects, and used isometric and isoinertial strength variables as predictors to develop prediction models. Also, the oxygen consumption, heart rate, and RPE were measured or recorded while subjects were lifting their MAWL. Three different lifting frequencies(1, 3, 5lifts/min) with two lifting range from floor to knuckle height and knuckle to shoulder height for one hour's work shift using free style lifting technique were studied. These results may not only provide scientific data in establishing the safety standards for Korean workers' lifting tasks, but also contribute preventing the rapidly increasing muscular-skeletal disorders lately on the industrial site.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.43 no.3
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• pp.10-20
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• 2020

This paper presents a method to measure the similarity of assigned jobs in the gravure printing operation based on the chromaticity and color sequence, and order the jobs accordingly. The proposed dispatching rule can be used to fulfill diverse manufacturing site requirements because the parameters can be adjusted to prioritize chromaticity and color sequence. In general, dispatching rules either ignore the job-changing time or require that the time be clearly defined. However, in the gravure printing operation targeted in this study, it is difficult to apply the general dispatching rule because of the difficulties in quantifying the job-changing time. Therefore, we propose a method for generalizing assignment rules of the job planner, allocating relative similarity among assigned jobs, and determining the sequence of jobs accordingly. Chromaticity priority is determined by the arrangement of the color assignments in the printing operation; color sequence priority is determined by the addition, deletion, or change in a specific color sequence. Finally, the job similarity is determined by the dot product of the chromaticity and color sequence priorities. Implementation of the proposed dispatching rule at an actual manufacturing site showed the planner present the same job order as that obtained using the proposed rule. Therefore, this rule is expected to be useful in industrial sites where clear quantification of the job-changing time is not possible.

• The Korean Journal of Emergency Medical Services
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• v.26 no.3
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• pp.201-208
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• 2022

Purpose: The purpose of this study is to analyze the current status of disasters and initial first aid in the construction industry, and to suggest the deployment of emergency medical technicians and reinforcement of first aid training in the construction industry. Methods: Among industrial accidents in 2020, the accident characteristics and initial first aid that occurred at construction sites were analyzed, and the emergency dispatch of 119 paramedics was analyzed through the SPSS method. Results: The incidence of serious accidents was high at small and medium-sized construction sites with less than 50 workers, and the incidence of falls was high, and there were few first responders who could provide first aid in the beginning. Therefore, it is necessary to improve first aid response capabilities at small construction sites. Conclusion: In order to improve the ability to respond to first aid at construction sites, it is necessary to deploy first responders and strengthen first aid education.

• Journal of Engineering Education Research
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• v.20 no.5
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• pp.3-13
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• 2017

The purpose of this study was to investigate the effect that completing long-term on-site training with varying grades of success had on the job satisfaction of former university students after obtaining employment. Although the grades themselves achieved during long-term on-site training did not directly affect job satisfaction, positive effects on job satisfaction were shown due to the mediating effects of job match and organizational commitment. The results of this study are as follows: First, it was found that employees who participated in long-term on-site training showed different results in belonging and attachment to their organizations according to their achievement. The higher the grades during long-term on-site training, the higher the degree of reported job match and organizational commitment, suggesting a positive correlation with job satisfaction. Therefore, long-term on-site training should not be operated as simply a program for temporary experiential learning, but instead should be optimized by companies and schools to motivate their students to achieve high grades for the purpose of their future job satisfaction.

• Journal of the Korean Institute of Landscape Architecture
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• v.34 no.6 s.119
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• pp.111-129
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• 2007

Industrial heritage artifacts may include the industrial products, technologies and infrastructures that have contributed to modernization beginning with the Industrial Revolution. The history of our industrial heritage spans 50 to 150 years and can be characterized by taking into account the site and the technology. This paper analyzes 13 precedents in Japan, Canada, and the United States in terms of these concerns, with focus on the reuse of abandoned mines as industrial heritage. Field surveys and interviews about each abandoned mine were used to obtain historical records and material. The results describe progress in three phases (1) recognizing phase, (2) organizing phase, and (3) maintenance management phase. A proper methodology for reuse is necessary to ensure the authenticity of the abandoned mine, particularly in the face of poor tourism-oriented approaches. As a result of analyzing the 13 cases, we determined that the following principles should be considered during the reuse process. Firstly, reuse of abandoned mines should not be compulsory but should be a spontaneous process and especially, should be carried out by inhabitants. Secondly, education and real experiences in the abandoned mine should be used to ive visitors a feeling of authenticity. Thirdly, creative remodeling methods can be used to enhance the abandoned mine's facilities and the site. Finally, historic and new functions should be the focus of the revitalization. Because this paper mainly focused on 13 precedents, there are likely more diverse cases. However, the conclusions of this report have practical value for reuse of abandoned mines and can be used in establishing methods for reusing Korean abandoned mines as industrial heritage.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.12
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• pp.6550-6557
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• 2013

The public road construction projects awarded by the regional construction and management office, which is an affiliate of the Ministry of Land, Infrastructure and Transport, are managed by construction supervision officers. These officials frequently visit a large number of construction sites to conduct inspections and supervision tasks. Therefore, the site management efficiency is essential in terms of the time and money spent in travelling to the sites. The introduction of a site monitoring management system is considered necessary to minimize the number of site visits and enable remote monitoring of the construction progress to enhance the business efficiency of the construction supervision officers. In this study, a remote site monitoring system was constructed using web cameras for public road construction works. The trial applications were implemented by selecting ten constructions sites. The effectiveness of the system was analyzed to assess its applicability. In an assessment of the applicability of the verification results, remote site monitoring showed cost savings of approximately 35% compared to the existing site management. The guidelines for applying the site monitoring management system were provided, the introduction plan was investigated, and the improvement method was presented. The results showed that the system is likely to minimize the unnecessary site visits, remove the risk factors at vulnerable areas in the sites beforehand, and prevent a range of disasters and accidents. In addition, the quality of the infrastructures is likely to improve through the prevention of accidents and the elimination of substandard and faulty construction work.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.12
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• pp.1161-1168
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• 2009

This research was conducted on characteristic of offensive odors in Shihwa industrial complex. Result of blocks distribution of TVOC indicates that mechanic block, site D, was the highest concentration (74 ppb). Chemistry block, site A, was the second highest concentration (50 ppb). Also, mixed blocks, metal blocks and park etc. were measured almost similar concentration about 30 ppb, but mixed block, site F, was the place where concentrations were the smallest. Average of TVOC was shown about 35 ppb concentration. Aldehydes including acetaldehyde, butyraldehyde and hydrogen sulfide concentrations were prevalent among offensive odors in Shihwa industrial complex. Comparing the offensive odor intensity mostly about acetaldehyde, butyraldehyde and hydrogen sulfide which contain high offensive odor intensity showed results that sites A, B (chemistry block) and site D, I (mechanic block) site H (metal block) have showed the intensity over 1. In the case of acetaldehyde, relatively the high odor intensities over '2' were able to obtain in many cases. The correlation coefficient (r) for hydrogen sulfide was 0.91, so that high positive correlation exists between offensive odor intensity and the hydrogen sulfide element. Butyraldehyde also showed high positive correlation coefficient, as 0.82. Correlation coefficient of acetaldehyde that had the highest value as offensive odor substance was 0.62, had somewhat correlation with offensive odor intensity.

• Journal of Ecology and Environment
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• v.32 no.2
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• pp.115-121
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• 2009

In order to elucidate the mechanisms affecting plant distributions in the reclaimed dredging area in the Gwangyang steelworks, in the Gwangyang Bay, Korea, we examined soil characteristics and plant distributions in four study sites and a control site in the study area. Desalination occurring along a gradient with increasing elevation, resulting in decrease of soil pH, EC, P, K, Cl, Ca, Mg, and salt and an increase in soil T-N, silt, clay contents. From site 1 (the lowest-elevation site) to site 5 (the highest-elevation site), halophytes decreased in abundance and nonhalophytes increased. The dominant species in each site were: Phragmites communis, Limonium tetragonum, and 12 additional species at site 1, Carex pumila, Suaeda japonica, and 15 additional species at site 2, Spergularia marina, Scirpus planiculmis, and 22 additional species at site 3, Miscantus sinensis, Lespedeza bicolor, and 26 additional species at site 4 and Pinus thunberii, Rhododendron mucronulatum, and 39 additional species at site 5, which resembled a naturally-occurring P. thinbergii community. Cluster analysis of the vegetation data matrix grouped the 35 plots into 5 major groups, and cluster analysis using the soil environment data matrix revealed 4 major groups. CCA of the floristic and environmental data matrix showed a positive relationship of SAR, EC, Na, Cl, and Ca, which are related to salt, in the $1^{st}$ axis and $2^{nd}$ axis, but negative relationships for altitude, organic contents, silt, and clay contents. Notably, plant species in the reclaimed dredging area that were separated along the $1^{st}$ axis showed strong relationships with factors that related to salt. Long-term exposure to natural rainfall in the reclaimed dredging area changed the soil characteristics, such as salinity. This change in soil characteristics might alter the SAR, which affects plant survival strategies in a given habitat. These results strongly indicated that factors related to salt and elevation play important roles in determining the overall plant distribution in the reclaimed dredging area.

• Journal of Korea Society of Industrial Information Systems
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• v.25 no.2
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• pp.19-27
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• 2020

Recently, rapid and accurate 3D models creation is required in various applications using virtual reality and augmented reality technology. In this paper, we propose an on-site learning based shape deformation method which transforms the clothed 3D human model into the shape of an input point cloud. The proposed algorithm consists of two main parts: one is pre-learning and the other is on-site learning. Each learning consists of encoder, template transformation and decoder network. The proposed network is learned by unsupervised method, which uses the Chamfer distance between the input point cloud form and the template vertices as the loss function. By performing on-site learning on the input point clouds during the inference process, the high accuracy of the inference results can be obtained and presented through experiments.